Muscle DamageNCT01290458
The Effect of Antioxidant Vitamin Supplementation on Muscle Performance and Redox Status After Eccentric Training
A study testing Antioxidant vitamins in people with Muscle Damage and Oxidative Stress, run by University of Thessaly at 1 site in Greece.
Trial summary
Consumption of vitamin supplements is a common practice among athletes or people participating in health promoting exercise programs. The reason for this interest in vitamin supplements is primarily because of the observation that enhanced production of reactive oxygen and nitrogen species (RONS) influence fundamental… Read more about this study
- Who is it for?
- Ages 18 to 30 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Muscle Damage, Oxidative Stress, Hemolysis
- What is being tested?
- Antioxidant vitamins
- Type of treatment
- Pill or oral medicine
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 28 participants
- Where
- 1 siteGreece
- Study dates
- Jun 2009to Jun 2010
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 30 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Physiological body mass index (BMI)
- Physiological health profile
- Subject provides written informed consent
Who cannot join
- Smoker
- Professional athlete
- Consumed any nutritional supplement the last 3 months
- Performed pure eccentric exercise the last 6 months
- Non Caucasian
Trial locations and contacts
1 site in 1 country.
About this study
Consumption of vitamin supplements is a common practice among athletes or people participating in health promoting exercise programs. The reason for this interest in vitamin supplements is primarily because of the observation that enhanced production of reactive oxygen and nitrogen species (RONS) influence fundamental biological processes, such as gene expression, signal transduction and enzyme activity. In a muscle and exercise physiology context, a low level of RONS is required for normal force production, whereas marked increases in RONS can cause contractile dysfunction, resulting in muscle weakness and fatigue. On the other hand RONS are involved in signaling pathways and serve to up-regulate the expression of a number of genes and can exert favorable effects such as training adaptations.
The present study will employ a valid eccentric exercise model to examine the influence of combined vitamin C and E supplementation after acute and chronic eccentric exercise on muscle damage and performance, redox status, hemolysis and lipid and lipoprotein profile.
Read the detailed description
Historically there was a shift in the paradigm regarding the effects of antioxidant supplementation on muscle performance and redox status. In fact, back in the 80's and 90's most of the relevant studies reported "positive" effects of antioxidant supplementation on muscle performance, muscle damage and redox status. On the other hand, the last five years, an increasing number of well-received studies are appearing pointing towards the negative impact of antioxidant supplementation. Moreover, much more studies than the past are now specifically addressing the effects of antioxidant supplementation on the exercise adaptations that take place after chronic exercise. Regarding the latter, it has been reported recently that antioxidant supplementation greatly decreases training efficiency and prevents many cellular adaptations to chronic exercise. Nevertheless, the debate is still open and an equal number of recent studies have reported the reverse i.e. positive effects of antioxidant supplementation on exercise adaptations or virtually no effect of antioxidant supplementation on exercise adaptations.
Eccentric muscle work is an essential part of daily activities of humans, such as walking, and in particular, when walking downhill or descending stairs. The most notable and well-described effect of eccentric exercise is the muscle damage that peaks one to three days after exercise. Eccentric exercise can cause effects other than just muscle damage, from activation of several transcription factors to favorable changes on lipid and lipoproteins profile.
In a double-blinded fashion, men will receive either a daily oral supplementation of vitamin C (1g) and vitamin E (400IU) or placebo for eleven weeks. Following baseline tests, volunteers will have to perform an eccentric exercise session two times per week for four weeks. Before and after the chronic eccentric exercise volunteers will be subject to one session of acute eccentric exercise, and physiological measurements will be performed as well as blood samples and muscle biopsies will be collected.
The aims of the present research are to investigate:
- the effect of antioxidant vitamin supplementation for 4 weeks on muscle performance, redox status, hemolysis and lipids and lipoprotein profile,
- the effect of an acute bout of eccentric exercise on muscle performance, redox status, hemolysis and lipid and lipoproteins profile of untrained individuals supplemented with or without antioxidant vitamins,
- the effect of 4 weeks of eccentric training on muscle performance, redox status, hemolysis and lipid and lipoproteins profile of untrained individuals supplemented with or without antioxidant vitamins,
- the effect of an acute bout of eccentric exercise on muscle performance, redox status, hemolysis and lipid and lipoproteins profile of individuals trained for 4 weeks and supplemented with or without antioxidant vitamins.
- Conditions
- Muscle Damage, Oxidative Stress, Hemolysis
- Being tested
- Antioxidant vitamins
- Keywords
- Eccentric training, Free radicals, Antioxidant supplements, Training adaptations, Lipid profile
- Medical subject headings
- Hemolysis
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Antioxidant vitaminsDietary supplement
Also called For vitamin C ascorbic acid, For vitamin E d-alpha tocopherol
Oral supplementation with one tablet of 1 g of vitamin C (Lamberts Healthcare Ltd, UK) and one tablet of 400 IU of vitamin E (Lamberts Healthcare Ltd, UK)
| Study group | Role | What they receive |
|---|---|---|
| Vitamin | Experimental | Not described Receives: Dietary Supplement: Antioxidant vitamins |
| Control | Placebo comparator | Not described Receives: Dietary Supplement: Antioxidant vitamins |
What is being measured
The primary outcome is the main question the study is designed to answer.
Maximum isometric torque (torque)
An isokinetic dynamometer (Cybex, Ronkonkoma, NY) will be used for the measurement of isometric knee extensor peak torque at 90° knee flexion. The average of the three best maximal voluntary contractions with their dominant leg will be recorded. In order to ensure that the subjects provide their maximal effort, the measurements will be repeated if the difference between the lower and the higher torque value exceeded 10%. There will be two minutes rest between isometric efforts.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Range of motion, ROM (degrees)
The assessment of pain-free ROM will be performed manually using the the isokinetic dynamometer. The investigator will move the calf at a very low angular velocity from 0 knee extension to the position where the subject will feel any discomfort.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Delayed onset muscle soreness, DOMS (scale 1-10)
Each participant will assess delayed onset muscle soreness (DOMS) during squat movement (90o knee flexion) and perceived soreness will be rated on a scale ranging from 1 (normal) to 10 (very sore).
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Creatine kinase, CK (activity IU)
CK activity will be measured as a general indicator of muscle damage.It will be measured in a Cobas Integra Plus 400 chemistry analyzer. CK will be measured before the beginning of the supplementation (week 0), before and the week after the first bout of eccentric exercise (week 5), before and the week after the second bout of eccentric exercise (week 11).
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Secondary outcomes (15)
Reduced glutathione, GSH (μmol/g Hb)
GSH will be measured as a general index of oxidative stress. For GSH, 20 μL of erythrocyte lysate will be treated with 5% TCA mixed with 660 μL of 67 mM sodium potassium phosphate (pH 8.0) and 330 ΜL of 1 mM 5,5-dithiobis-2 nitrobenzoate. The samples will be incubated in the dark at room temperature for 45 min, and the absorbance will be read at 412 nm.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Oxidized glutathione, GSSG (μmol/g Hb)
GSSG will be measured as a general index of oxidative stress. It will be assayed by treating 50 μL of erythrocyte lysate with 5% TCA and neutralized up to pH 7.0-7.5. One microliter of 2-vinylpyridine will be added, and the samples will be incubated for 2 h. Sample will be treated with TCA and will be mixed with 600 μL of 143 mM sodium phosphate 100 ΜL of 3 mM NADPH, 100 ΜL of 10 mM 5,5-dithiobis-2-nitrobenzoate, and 194 μL of distilled water. After the addition of 1 μL of glutathione reductase, the change in absorbance at 412 nm will be read for 3 min.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Thiobarbituric acid-reactive substances, TBARS (μM)
TBARS will be measured as an index of lipid peroxidation. For TBARS determination, 100 μL of plasma will be mixed with 500 ΜL of 35% TCA and 500 μL of Tris-HCl (200 mM, pH 7.4) and will be incubated for 10 min at room temperature. One milliliter of 2 M Na2SO4 and 55 mM thiobarbituric acid solution will be added, and the samples will be incubated at 95 C for 45 min. The samples will be cooled on ice for 5 min and then will be vortexed after adding 1 mL of 70% TCA. The samples will be centrifuged at 15,000g for 3 min, and the absorbance of the supernatant will be read at 530 nm.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Protein carbonyls (nmol/mg pr.)
Carbonyls will be measured as an index of protein oxidation. Protein carbonyls will be determined adding 50 μL of 20% TCA to 50 μL of plasma. Samples will be incubated in the dark at room temperature for 1 h. The supernatant will be discarded, and 1 mL of 10% TCA will be added. The supernatant will be discarded, and 1 mL of ethanol-ethyl acetate will be added and centrifuged. The supernatant will be discarded, and 1 mL of 5 M urea will be added, vortexed, and incubated at 37C for 15 min. The samples will be centrifuged at 15,000g for 3 min at 4C, and the absorbance will be read at 375 nm.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Catalase (μmol/min/mg Hb)
Catalase will be measured as one of the main antioxidant enzyme of erythrocytes. Catalase activity will be determined adding 4 μL of erythrocyte lysate, 2955 μL of 67 mM sodium potassium phosphate (pH 7.4), and the samples will be incubated at 37C for 10 min. Five microliters of 30% hydrogen peroxide was added to the samples, and the change in absorbance will immediately read at 240 nm for 1.5 min.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Total antioxidant capacity, TAC (mm DPPH)
TAC will be determined adding 20 μL of plasma to 480 ΜL of 10 mM sodium potassium phosphate (pH 7.4) and 500 μL of 0.1 mM 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical, and the samples will be incubated in the dark for 30 min at room temperature. The samples will be centrifuged for 3 min at 20,000g, and the absorbance will be read at 520 nm.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Albumin (g/L)
Albumin will be measured as an antioxidant component of blood plasma. It will be determined spectrophotometrically based on the formation of a coloured complex with bromocresol green reagent.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Uric acid (μm)
Uric acid will be measured as the main antioxidant component of blood plasma. It will be measured in a Cobas Integra Plus 400 chemistry analyzer.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Vitamin C and E (mmol/L)
Vitamin C will be measured in plasma using a ferric reducing ascorbate assay kit using spectrophotometry. Vitamin E will be measured in plasma with the use of HPLC.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Plasma hemoglobin (g/dL)
Plasma hemoglobin will be assayed with a commercial kit using spectrophotometry as a hemolysis index.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Bilirubin (μM)
Bilirubin will be measured in a Cobas Integra Plus 400 chemistry analyzer as a hemolysis index.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Triacylglycerols, TG (μM)
TG will be measured in a Cobas Integra Plus 400 chemistry analyzer.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Total cholesterol (μM)
TC will be measured in a Cobas Integra Plus 400 chemistry analyzer.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
High-density lipoprotein cholesterol, HDLC (μM)
HDLC will be measured in a Cobas Integra Plus 400 chemistry analyzer.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Low-density lipoprotein cholesterol, LDLC (μM)
LDLC will be measured in a Cobas Integra Plus 400 chemistry analyzer.
Measured: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted7 Feb 2011 (estimated)
- Study startJun 2009
- Primary completionNov 2009
- Study completionJun 2010
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Publications (3)Papers the sponsor has linked to this study.
- Other trials for Muscle DamageStudies of the same condition, open and under way.
- More trials run by University of ThessalyThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Masking
- Double blind
- Who is masked
- Participant, Investigator
- Enrolment
- 28 participants (actual)
Sponsor and investigators
- Lead sponsor
- University of Thessaly
- Sponsor type
- University, hospital or other
- Collaborators
- European University Cyprus, Greek Ministry of Development, Democritus University of Thrace
- Investigators
- Athanasios Z Jamurtas, Dr, Study chair, University of Thessaly
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT01290458
- Sponsor's protocol ID
- UTH-2008
- Status last verified
- Jan 2011
- First submitted
- 4 Feb 2011
- Last updated
- 7 Feb 2011
- Results posted
- No
- Data monitoring committee
- Yes
Publications
Paschalis V, Nikolaidis MG, Theodorou AA, Panayiotou G, Fatouros IG, Koutedakis Y, Jamurtas AZ. A weekly bout of eccentric exercise is sufficient to induce health-promoting effects. Med Sci Sports Exerc. 2011 Jan;43(1):64-73. doi: 10.1249/MSS.0b013e3181e91d90.
PubMed 20508540Paschalis V, Nikolaidis MG, Giakas G, Theodorou AA, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ. Beneficial changes in energy expenditure and lipid profile after eccentric exercise in overweight and lean women. Scand J Med Sci Sports. 2010 Feb;20(1):e103-11. doi: 10.1111/j.1600-0838.2009.00920.x. Epub 2009 Apr 14.
PubMed 19422638Nikolaidis MG, Paschalis V, Giakas G, Fatouros IG, Sakellariou GK, Theodorou AA, Koutedakis Y, Jamurtas AZ. Favorable and prolonged changes in blood lipid profile after muscle-damaging exercise. Med Sci Sports Exerc. 2008 Aug;40(8):1483-9. doi: 10.1249/MSS.0b013e31817356f2.
PubMed 18614942
Study at a glance
From the registry record, last updated by the sponsor on 7 Feb 2011. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Effect of Antioxidant Vitamin Supplementation on Muscle Performance and Redox Status After Eccentric Training
- ClinicalTrials.gov ID
- NCT01290458
- Acronym / sponsor ID
- UTH-2008
- Conditions
- Muscle Damage, Oxidative Stress, Hemolysis
- Keywords
- Eccentric training, Free radicals, Antioxidant supplements, Training adaptations, Lipid profile
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 28 participants (actual)
- Lead sponsor
- University of ThessalyUniversity, hospital or other
- Collaborators
- European University Cyprus, Greek Ministry of Development, Democritus University of Thrace
- Study start
- Jun 2009
- Primary completion
- Nov 2009 (actual)
- Study completion
- Jun 2010 (actual)
- First posted
- 7 Feb 2011 (estimated)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Consumption of vitamin supplements is a common practice among athletes or people participating in health promoting exercise programs. The reason for this interest in vitamin supplements is primarily because of the observation that enhanced production of reactive oxygen and nitrogen species (RONS) influence fundamental biological processes, such as gene expression, signal transduction and enzyme activity. In a muscle and exercise physiology context, a low level of RONS is required for normal force production, whereas marked increases in RONS can cause contractile dysfunction, resulting in muscle weakness and fatigue. On the other hand RONS are involved in signaling pathways and serve to up-regulate the expression of a number of genes and can exert favorable effects such as training adaptations.
The present study will employ a valid eccentric exercise model to examine the influence of combined vitamin C and E supplementation after acute and chronic eccentric exercise on muscle damage and performance, redox status, hemolysis and lipid and lipoprotein profile.
Detailed description
Historically there was a shift in the paradigm regarding the effects of antioxidant supplementation on muscle performance and redox status. In fact, back in the 80's and 90's most of the relevant studies reported "positive" effects of antioxidant supplementation on muscle performance, muscle damage and redox status. On the other hand, the last five years, an increasing number of well-received studies are appearing pointing towards the negative impact of antioxidant supplementation. Moreover, much more studies than the past are now specifically addressing the effects of antioxidant supplementation on the exercise adaptations that take place after chronic exercise. Regarding the latter, it has been reported recently that antioxidant supplementation greatly decreases training efficiency and prevents many cellular adaptations to chronic exercise. Nevertheless, the debate is still open and an equal number of recent studies have reported the reverse i.e. positive effects of antioxidant supplementation on exercise adaptations or virtually no effect of antioxidant supplementation on exercise adaptations.
Eccentric muscle work is an essential part of daily activities of humans, such as walking, and in particular, when walking downhill or descending stairs. The most notable and well-described effect of eccentric exercise is the muscle damage that peaks one to three days after exercise. Eccentric exercise can cause effects other than just muscle damage, from activation of several transcription factors to favorable changes on lipid and lipoproteins profile.
In a double-blinded fashion, men will receive either a daily oral supplementation of vitamin C (1g) and vitamin E (400IU) or placebo for eleven weeks. Following baseline tests, volunteers will have to perform an eccentric exercise session two times per week for four weeks. Before and after the chronic eccentric exercise volunteers will be subject to one session of acute eccentric exercise, and physiological measurements will be performed as well as blood samples and muscle biopsies will be collected.
The aims of the present research are to investigate:
- the effect of antioxidant vitamin supplementation for 4 weeks on muscle performance, redox status, hemolysis and lipids and lipoprotein profile,
- the effect of an acute bout of eccentric exercise on muscle performance, redox status, hemolysis and lipid and lipoproteins profile of untrained individuals supplemented with or without antioxidant vitamins,
- the effect of 4 weeks of eccentric training on muscle performance, redox status, hemolysis and lipid and lipoproteins profile of untrained individuals supplemented with or without antioxidant vitamins,
- the effect of an acute bout of eccentric exercise on muscle performance, redox status, hemolysis and lipid and lipoproteins profile of individuals trained for 4 weeks and supplemented with or without antioxidant vitamins.
Study design
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Masking
- Double blind (participant, investigator)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| Vitamin | Experimental | Dietary Supplement: Antioxidant vitamins |
| Control | Placebo comparator | Dietary Supplement: Antioxidant vitamins |
Who may qualify
- Ages
- 18 to 30 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (3 listed)
- Physiological body mass index (BMI)
- Physiological health profile
- Subject provides written informed consent
Key exclusion criteria (5 listed)
- Smoker
- Professional athlete
- Consumed any nutritional supplement the last 3 months
- Performed pure eccentric exercise the last 6 months
- Non Caucasian
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Antioxidant vitaminsAlso: For vitamin C ascorbic acid, For vitamin E d-alpha tocopherolOral supplementation with one tablet of 1 g of vitamin C (Lamberts Healthcare Ltd, UK) and one tablet of 400 IU of vitamin E (Lamberts Healthcare Ltd, UK) | Dietary supplement | Control, Vitamin |
Primary outcome
Maximum isometric torque (torque)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
An isokinetic dynamometer (Cybex, Ronkonkoma, NY) will be used for the measurement of isometric knee extensor peak torque at 90° knee flexion. The average of the three best maximal voluntary contractions with their dominant leg will be recorded. In order to ensure that the subjects provide their maximal effort, the measurements will be repeated if the difference between the lower and the higher torque value exceeded 10%. There will be two minutes rest between isometric efforts.
Range of motion, ROM (degrees)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
The assessment of pain-free ROM will be performed manually using the the isokinetic dynamometer. The investigator will move the calf at a very low angular velocity from 0 knee extension to the position where the subject will feel any discomfort.
Delayed onset muscle soreness, DOMS (scale 1-10)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Each participant will assess delayed onset muscle soreness (DOMS) during squat movement (90o knee flexion) and perceived soreness will be rated on a scale ranging from 1 (normal) to 10 (very sore).
Creatine kinase, CK (activity IU)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
CK activity will be measured as a general indicator of muscle damage.It will be measured in a Cobas Integra Plus 400 chemistry analyzer. CK will be measured before the beginning of the supplementation (week 0), before and the week after the first bout of eccentric exercise (week 5), before and the week after the second bout of eccentric exercise (week 11).
15 secondary outcomes
Reduced glutathione, GSH (μmol/g Hb)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Oxidized glutathione, GSSG (μmol/g Hb)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Thiobarbituric acid-reactive substances, TBARS (μM)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Protein carbonyls (nmol/mg pr.)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Catalase (μmol/min/mg Hb)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Total antioxidant capacity, TAC (mm DPPH)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Albumin (g/L)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Uric acid (μm)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Vitamin C and E (mmol/L)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Plasma hemoglobin (g/dL)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Bilirubin (μM)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Triacylglycerols, TG (μM)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Total cholesterol (μM)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
High-density lipoprotein cholesterol, HDLC (μM)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Low-density lipoprotein cholesterol, LDLC (μM)
Time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 7 Feb 2011.
- Background: PubMed 20508540Paschalis V, Nikolaidis MG, Theodorou AA, Panayiotou G, Fatouros IG, Koutedakis Y, Jamurtas AZ. A weekly bout of eccentric exercise is sufficient to induce health-promoting effects. Med Sci Sports Exerc. 2011 Jan;43(1):64-73. doi: 10.1249/MSS.0b013e3181e91d90.
- Background: PubMed 19422638Paschalis V, Nikolaidis MG, Giakas G, Theodorou AA, Sakellariou GK, Fatouros IG, Koutedakis Y, Jamurtas AZ. Beneficial changes in energy expenditure and lipid profile after eccentric exercise in overweight and lean women. Scand J Med Sci Sports. 2010 Feb;20(1):e103-11. doi: 10.1111/j.1600-0838.2009.00920.x. Epub 2009 Apr 14.
- Background: PubMed 18614942Nikolaidis MG, Paschalis V, Giakas G, Fatouros IG, Sakellariou GK, Theodorou AA, Koutedakis Y, Jamurtas AZ. Favorable and prolonged changes in blood lipid profile after muscle-damaging exercise. Med Sci Sports Exerc. 2008 Aug;40(8):1483-9. doi: 10.1249/MSS.0b013e31817356f2.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Athanasios Z Jamurtas, Dr study chair
University of Thessaly
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Antioxidant vitamins in people with Muscle Damage and Oxidative Stress, run by University of Thessaly at 1 site in Greece.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 3 inclusion and 5 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 7 Feb 2011. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Greece. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Maximum isometric torque (torque) (time frame: Before the beginning of the supplementation (week 0), the week after the first bout of eccentric exercise (week 5), and the week after the second bout of eccentric exercise (week 11)). The study lists 4 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
On the ClinicalTrials.gov record for NCT01290458, which also lists 3 linked publications.
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 7 Feb 2011; AzTrials last refreshed its copy on 8 Nov 2026.