Gut Microbial Transplantation in Pediatric Inflammatory Bowel Diseases

Participant Inclusion Criteria:

1. Children ages >7 and ≤21 years of age with established diagnosis of Inflammatory Bowel Disease (IBD) colitis (patient with ulcerative colitis (UC) or patients with crohn's disease (CD) with colonic involvement only)
2. Have clinical disease (mild to moderate disease: 10≤ Pediatric Ulcerative Colitis Activity Index (PUCAI) <65)
3. Have stable disease activity and therapy for two months prior to Gut Microbial Transplantation (GMT) procedure.

Participant Exclusion Criteria:

1. Fulminant colitis
2. Indication or scheduled for surgery
3. Pregnancy
4. Use of probiotic supplements during the study period (subjects who have stopped use of probiotic supplements will be eligible as long as they stop taking it 2 weeks prior to Day 1 of GMT)
5. anemia (hemoglobin < 6.0 g/dL) in last one month
6. Graft versus host disease (GVHD)
7. Severely immunocompromised - defined as
8. History of opportunistic infection (tuberculosis, Pneumocystis jirovecii or systemic fungal infections) in last one year or
9. Neutropenia: Absolute neutrophil count (ANC) <500
10. Major intra-abdominal surgery within 90 days prior to Day 1 of GMT
11. Administration of any investigational drug within 30 days prior to Day 1 of GMT.
12. Have received infliximab or other tumor necrosis factor (TNF) inhibitors within 2 months prior to Day 1 of GMT or are expected to receive such therapy within 1 month post final GMT.

Donor Inclusion Criteria

1. Based on patient's and parents'/guardian's decision
2. Will be chosen from immediate adult (≥18 years) family members or close friends
3. Should have negative or normal results on screening tests (as explained in donor exclusion criteria below)

Donor Exclusion Criteria (adopted from guidelines provided by American Association of Blood Bank Donor History Questionnaire and American Gastroenterological Association)

1. Positive or abnormal screening test to include:
2. Hepatitis A Immunoglobulin M (IgM) antibody
3. Hepatitis B antibody panel suggestive of infection (HBc IgM, HBs Ag, HBs Ab)
4. Hepatitis C antibody Level
5. Cytomegalovirus (CMV) IgM antibody
6. Ebstein Barr Virus (EBV) viral capsid antigen (VCA) IgM antibody
7. Syphilis Immunoglobulin G (IgG) Ab Screen
8. Human Immune Deficiency Virus (HIV) I \& II enzyme-linked immunosorbent assay (ELISA) screen
9. Stool i) Fungal smear ii) Stool cultures to exclude Salmonella, Shigella, Escherichia Coli, Campylobacter, Yersinia, Vibrio, Listeria iii) Clostridium difficile toxin assay iv) Ova and parasite screen for Giardia and Cryptosporidium
10. History of metabolic syndrome or gastric bypass surgery
11. History of GI conditions: IBD, irritable bowel syndrome, chronic abdominal pain, GI Malignancy, blood in stool or diarrhea in the last 4 weeks before enrollment
12. Antibiotic or probiotic use within 3 months before enrollment and for the duration of the donation
13. Positive response on screening questionnaire to any of the following:
14. History of active malignancy or any cancer within the last 5 years (excluding basal cell carcinoma of the skin)
15. Family history of Creutzfeld-Jacob disease
16. Corneal or dural transplant
17. History of high risk sexual behavior (e.g. sexual contact with HIV/acquired immune deficiency syndrome (AIDS) positive, hepatitis positive partner, men who have sex with men, sex for drugs or money)
18. History of signs of sexually transmitted infections such as genital ulcerations, anogenital herpes, anogenital warts, cancroids or syphilitic lesions
19. Use of illicit drugs
20. Travel within the last 6 months to areas of the world where diarrheal illnesses are endemic
21. History or signs of immunosuppression i) History of any immunosuppressant medication(s) within the last 6 months or history of opportunistic infection(s) in past 1 year ii) Signs of immunosuppression such as: <!-- -->
22. Oral thrush
23. Disseminated lymphadenopathy