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AtherosclerosisPhase 1NCT02058641

Effect of Darapladib on Cantharidin-Induced Inflammatory Blisters in Subjects With Type 2 Diabetes Mellitus (T2DM)

A Phase 1 study testing Darapladib in people with Atherosclerosis, run by GlaxoSmithKline at 1 site in United Kingdom.

Completed

Finished. The study has ended and is not enrolling. Results may be posted on the official record.

Official record
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Trial summary

This will be an exploratory, open-label, single sequence, two part study (Part A and an optional Part B). The aim of this study will be to assess whether systemic inhibition of Lipoprotein associated phospholipase A2 (Lp-PLA2) in humans, effected by 11 days of once daily dosing to steady state with 160 milligrams (mg)… Read more about this study

Who is it for?
Ages 18 to 60 years
Conditions the study is for
Atherosclerosis
What is being tested?
Darapladib
Type of treatment
Pill or oral medicine
Could I receive a placebo?
No, everyone receives an active treatment
How many people
9 participants
Where
1 siteUnited Kingdom
Study dates
26 Feb 2014to 18 Aug 2014
Phase
Phase 1
Are there results?
None posted in the registry

Key requirements

Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.

  • Ages 18 to 60 years
  • All sexes
  • No healthy volunteers
  • Adult

Who may be able to join

  • Male or female between 18 and 60 years of age inclusive, at the time of signing the informed consent.
  • A female subject is eligible to participate if she is of: non-childbearing potential defined as pre-menopausal females with a documented tubal ligation or hysterectomy; or postmenopausal defined as 12 months of spontaneous amenorrhea [in questionable cases a blood sample with simultaneous follicle stimulating hormone (FSH) > 40 milli-international units (MIU)/milliliter (mL) and estradiol < 40 picograms (pg)/mL (<147 picomoles per liter [pmol/L]) is confirmatory]. [Females on hormone replacement therapy (HRT) and whose menopausal status is in doubt will be required to use one of the contraception methods listed in the protocol if they wish to continue their HRT during the study. Otherwise, they must discontinue HRT to allow confirmation of post-menopausal status prior to study enrollment. For most forms of HRT, at least 2-4 weeks should elapse between the cessation of therapy and the blood draw; this interval depends on the type and dosage of HRT. Following confirmation of their post-menopausal status, they can resume use of HRT during the study without use of a contraceptive method]; Child-bearing potential and is abstinent (abstinence from penile-vaginal intercourse must be consistent with the preferred and usual lifestyle of the subject) or agrees to use one of the contraception methods listed in the protocol for an appropriate period of time (as determined by the product label or investigator) prior to the start of dosing to sufficiently minimize the risk of pregnancy at that point. Female subjects must agree to use contraception until 42 days after the last dose of study medication.
  • Body mass index (BMI) within the range of 19.0-35.0 kilograms per meter square (kg/ m^2) (inclusive).
  • QTc interval Fridericia correction (QTcF) <480 milliseconds (msec) in all subjects, including those with bundle branch block at screening electrocardiogram (ECG). Note that if the initial QTc value is prolonged, the ECG should be repeated two more times (with at least 5 minutes between ECG readings) and the average of the 3 QTc values used to determine eligibility.
  • Additional Criteria for Diabetic Subjects
  • A diagnosis of T2DM as determined by a responsible physician based on a medical evaluation including medical history, physical examination, and laboratory tests, with onset at least 6 months prior to screening and on stable treatment for 3 months prior to screening.
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  • Subjects have no recent changes or anticipation of future changes in anti-hyperglycaemic therapies during the 3-month period before and during the study respectively.
  • Subjects will have good peripheral pulses and no peripheral sensory loss as determined by physical examination.
  • Glycosylated hemoglobin (HbA1c) levels <= 8.0% at screening. Additional Criteria for Healthy Subjects (if recruited in Part B)
  • Healthy as determined by a responsible and experienced physician, based on a medical evaluation including medical history, physical examination, vital signs, complete blood count and clinical chemistry. A subject with a clinical abnormality or laboratory parameters outside the reference range for the population being studied may be included only if the Investigator and the GSK Medical Monitor agree that the finding is unlikely to introduce additional risk factors and will not interfere with the study procedures.

Who cannot join

  • A positive pre-study Hepatitis B surface antigen or positive Hepatitis C antibody result within 3 months of screening
  • Current or chronic history of liver disease or known hepatic or biliary abnormalities (with the exception of Gilbert's syndrome or asymptomatic gallstones). Abnormal liver function tests at screening. For healthy subjects: aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase and bilirubin >=1.5xupper limit of normal (ULN) (isolated bilirubin >1.5xULN is acceptable if bilirubin is fractionated and direct bilirubin <35%) at screening.
  • A positive pre-study drug/alcohol screen.
  • A positive test for HIV antibody.
  • History of regular alcohol consumption within 6 months of the study defined as: For United Kingdom (UK) sites: an average weekly intake of >21 units for males or >14 units for females. One unit is equivalent to 8 grams (g) of alcohol: a half-pint (approximately 240 mL) of beer, 1 glass (125 mL) of wine or 1 (25 mL) measure of spirits.
  • The subject has participated in a clinical trial and has received an investigational product within the following time period prior to the first dosing day in the current study: 90 days, 5 half-lives or twice the duration of the biological effect of the investigational product (whichever is longer).
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  • Exposure to more than four new chemical entities within 12 months prior to the first dosing day.
  • Unable to refrain from the use of prescription drugs taken on an intermittent (as needed) basis or non-prescription drugs; these include vitamins, herbal and dietary supplements (including St John's Wort) within 7 days (or 14 days if the drug is a potential enzyme inducer) or 5 half-lives (whichever is longer) prior to Day 1 of session 1 and continuing until the final follow up visit (with the exception of paracetamol).
  • History of sensitivity to any of the study medications, or components thereof or a history of drug or other allergy that, in the opinion of the Investigator or GSK Medical Monitor, contraindicates their participation.
  • History of anaphylaxis, and /or anaphylactoid (resembling anaphylaxis) reactions, or severe allergic responses.
  • Where participation in the study would result in donation of blood or blood products in excess of 500 mL within a 56 day period.
  • Currently in a study of an investigational device.
  • Pregnant females (as determined by positive serum beta human chorionic gonadotropin test at screening and on Day1 of Session 1, and pre-dose on day 1 of session 2) or lactating females.
  • Unwillingness or inability to follow the procedures outlined in the protocol.
  • Subject is mentally or legally incapacitated.
  • Unable to refrain from consumption grapefruit juice from 7 days prior to the first dose of study medication and until collection of the final sample in each session.
  • Subjects with both parents of Japanese, Chinese, or Korean ancestry.
  • Current smoker or former regular smoker within 6 months before the screening visit.
  • Previous exposure to darapladib (SB-480848).
  • Presence on either forearm of tattoos, naevi, scars, keloids, hyper- or hypo- pigmentation. Subjects with very fair skin, very dark skin, excessive hair or any skin abnormalities that may, in the opinion of the Investigator, interfere with study assessments.
  • Subjects with a history of keloids, skin allergy, hypersensitivity or contact dermatitis, including previous reactions to dressings to be used in the study, or any chronic skin disorder excepting isolated lesions (e.g. warts) remote from intended site of application of cantharidin.
  • Other issues which in the opinion of the investigator would preclude participation in the study.
  • Additional Criteria for Diabetic Subjects:
  • Abnormal liver function tests at screening. For T2DM subjects: ALT or AST >=2.5 x ULN or alkaline phosphatase or total bilirubin >=1.5 x ULN at screening. Current regular use of or anticipated requirement for anti-inflammatory medications (e.g. Non-steroidal anti-inflammatory drugs [NSAIDs] including low dose aspirin, glucocorticoids) and immune-modulatory therapies during the study.
  • Unable to refrain from the use of non-prescription drugs; these include vitamins, herbal and dietary supplements (including St John's Wort) within 7 days (or 14 days if the drug is a potential enzyme inducer) or 5 half-lives (whichever is longer) prior to Day 1 of session 1 and continuing until the final follow up visit. Anticipated addition of new therapies, or a change in the use of chronically administered prescription drugs, with the following exceptions: dose-adjustments for anti-diabetic medications, antihypertensive medications, or other changes that in the opinion of the Investigator and GSK Medical Monitor will not interfere with the study procedures, outcome or compromise subject safety.
  • Poorly controlled hypertension despite lifestyle modifications and pharmacotherapy, or on high dose statin therapy (e.g. equivalent to atorvastatin >40 mg/daily), and/or have a change in the type or dose of anti-hypertensive or statin medications during the 3-month period before and anticipated during the study. Poorly controlled hypertension refers to either systolic blood pressure >160 millimeters of mercury (mmHg) or diastolic blood pressure >110mmHg (mean of 3 measurements according to protocol-specified conditions). Patients may enter the study if the adjustment to blood pressure medications results in the improved control of hypertension at the Baseline visit).
  • Current or planned administration of strong oral or injectable cytochrome P-450 isoenzyme 3A4 (CYP3A4) inhibitors.
  • Severe renal impairment (e.g., patients with an estimated glomerular filtration rate <30 mL/minute/1.73 meter square or receiving chronic dialysis) or history of nephrectomy or kidney transplant (regardless of renal function).
  • Current severe heart failure (New York Heart Association class III or IV).
  • Subjects currently suffering from asthma (on the basis of excluding use of corticosteroids, even inhaled).
  • Co-morbid with chronic inflammatory conditions that could influence measurements of inflammation (including, but not limited to rheumatological disorders, e.g. systemic lupus erythematosus, and inflammatory bowel disease).
  • History of peripheral arterial disease and/or current use of warfarin or other anti-coagulants/anti-platelet agents (low dose aspirin / NSAIDs are not permitted) which, in the opinion of the Investigator and/or the GSK Medical Monitor, will interfere with the study procedures, outcome or compromise subject safety.
  • Subjects with a history of lymphangitis and/or lymphoedema or any subject who is considered to be at risk of these conditions e.g. has undergone surgery resulting in loss of tissue associated with normal lymphoid drainage (e.g. axillary lymph node dissection / excision related to breast surgery).

Trial locations and contacts

1 site in 1 country.

United Kingdom1 site

  • GSK Investigational Site

    Cambridge, CB2 0GG

Other trials in United Kingdom

About this study

Official title: An Open Label Study on the Effects of a Short Course of SB480848 (Darapladib) on Contents of Cantharidin-Induced Inflammatory Blisters in Subjects With Type 2 Diabetes Mellitus

This will be an exploratory, open-label, single sequence, two part study (Part A and an optional Part B). The aim of this study will be to assess whether systemic inhibition of Lipoprotein associated phospholipase A2 (Lp-PLA2) in humans, effected by 11 days of once daily dosing to steady state with 160 milligrams (mg) of enteric coated (EC) darapladib, will specifically reduce the number of macrophages and/or result in a higher proportion of M2 macrophages in skin blisters induced by cantharidin (a chemical agent that causes blisters). In Part A of the study, a cohort of 8 subjects with type 2 diabetes mellitus will be recruited. In Part B of the study, a cohort of 8 additional healthy subjects with matching age (+/- 24 months) and gender to Part A may be recruited.

Conditions
Atherosclerosis
Being tested
Darapladib
Keywords
cellular accumulation, macrophage phenotype, cantharidin blister, M1/M2, inflammatory mediators, Type 2 diabetes mellitus, darapladib, cytokines
Medical subject headings
Atherosclerosis, Diabetes Mellitus, Type 2

What participants receive

What participants receive. In a randomised study each person is assigned to one group by chance.

  • DarapladibDrug

    EC tablet with a unit dose strength of 160 mg administered once daily orally for 11 days.

Study groups in NCT02058641
Study groupRoleWhat they receive
Part AExperimentalPart A will consist of 2 sessions. In Session 1, 3 blisters will be induced by a challenging agent (cantharidin solution 0.2 %, with 5 microliter administered topically) in T2DM subjects on Day 1. Blisters will be harvested 48 (+/-2) hour (hr) post induction. In Session 2, the same subjects will be administered darapladib EC tablet 160 mg orally, once daily for 11 days. On Day 10, 3 blisters will be induced by cantharidin. Blisters will be harvested 48 (+/-2) hr post induction.
Receives: Drug: Darapladib
Part BExperimentalIn Part B, healthy subjects will be enrolled and will follow the same dosing procedure as in Part A. The decision to initiate Part B will be made by the GSK study team based on an evaluation of data from Part A.
Receives: Drug: Darapladib

What is being measured

The primary outcome is the main question the study is designed to answer.

  1. Macrophage cell count and surface expression of markers of M1 and M2 polarization in blister fluid

    To assess the effect of an 11 day course of once daily darapladib EC 160 mg on the number and phenotype (M1/M2 polarization) of macrophages isolated from blisters on subjects with type 2 diabetes mellitus (blisters induced by exposure to cantharidin for 48 hours). Biomarkers may include, but not be limited to: Cluster of differentiation (CD)11b, CD14, CD16, CD33, CD40, CD64, CD68, CD86, CD163, CD206, C-C chemokine receptor type 2 (CCR2), CX3 chemokine receptor 1 (CX3CR1)

    Measured: Up to 6 weeks

Secondary outcomes (5)

  1. Number of monocytes and surface expression of markers of M1 or M2 polarization in peripheral blood

    To assess the effect of an 11 day course of once daily darapladib EC 160 mg on the number and phenotype of monocytes (M1/M2 ratio) isolated from peripheral blood of subjects with type 2 diabetes mellitus. Biomarkers may include, but not be limited to: CD11b, CD14, CD16, CD40, CD64, CD68, CD163, CD206

    Measured: Up to 6 weeks

  2. Enzymatic activity of Lp-PLA2 in blister fluid

    Blood samples will be taken for assessments of enzymatic activity of Lp-PLA2 (measured by hydrolysis of radio-labeled platelet-activating factor [PAF])

    Measured: Up to 6 weeks

  3. Total cell count, lymphocyte count, neutrophil count and macrophage count in blister fluid

    Blister fluids will be collected to assess the differences in blister phenotype (volume, appearance, cellularity) between subjects

    Measured: Up to 6 weeks

  4. Concentrations of soluble mediators of macrophage polarization

    Soluble mediators will be measured in blister fluid assessed by a multiplex platform (Meso scale delivery or Luminex) and may include but not be restricted to matrix metalloproteinase 9 (MMP9), interleukin (IL)-12, IL-23, IL-10, interferon-gamma, tumor necrosis factor-alpha, IL-1 beta, IL-6, IL-8, IL-4, IL-13, transforming growth factor-beta, monocyte chemotactic protein-1 and C-reactive protein

    Measured: Up to 6 weeks

  5. Time to healing of cantharidin-induced blisters

    Blisters will be considered to be healed when the blister cover has fallen off and the blister area becomes dry

    Measured: Up to 8 weeks

Timeline

From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.

  1. First posted10 Feb 2014 (estimated)
  2. Study start26 Feb 2014
  3. Primary completion18 Aug 2014
  4. Study completion18 Aug 2014

Resources

More details

For doctors and researchers: how the study is designed, who runs it, and its registry IDs.

Study design
Study type
Interventional
Phase
Phase 1
Primary purpose
Basic science
Allocation
Non-randomised
Intervention model
Single group
Masking
None (open label)
Enrolment
9 participants (actual)
IDs, dates and publications
ClinicalTrials.gov ID
NCT02058641
Sponsor's protocol ID
200934
Status last verified
May 2017
First submitted
6 Feb 2014
Last updated
15 May 2017
Results posted
No
Data monitoring committee
No

Questions about this trial

What is this clinical trial studying?

A Phase 1 study testing Darapladib in people with Atherosclerosis, run by GlaxoSmithKline at 1 site in United Kingdom.

The full registered description is in the About this study section and the official record.

Who may be eligible to take part?

The sponsor lists 10 inclusion and 33 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.

Is this clinical trial currently recruiting?

The record says: finished, as of the sponsor's last update on 15 May 2017. Status can change between updates, so confirm with the study contact before making plans.

Where is the study taking place?

The record lists 1 site in United Kingdom. Whether a site is taking part right now should be confirmed with that site. See every site.

What are the possible benefits and risks?

They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.

What does this phase mean?

Phase 1 studies test a treatment in a small group of people for the first time or in a new way, mainly to check safety and find the right dose.

Not every study follows the same path, and some studies (observational studies, device studies) have no phase.

Are results available for this study?

The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.

How can a doctor refer a patient or contact the research team?

The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.

What does the primary outcome measure?

Macrophage cell count and surface expression of markers of M1 and M2 polarization in blister fluid (time frame: Up to 6 weeks). It is what the study was designed to measure, not a finding.

Where can I read the original study information?

On the ClinicalTrials.gov record for NCT02058641.

Does a listing mean the treatment is approved or proven to work?

No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.

How often is this information updated?

AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 15 May 2017.

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