Healthy ParticipantsNCT02882386
Amino Acid Kinetics in Blood After Consuming Different Milk Protein Supplements
A study testing Strength Training, Milk 1% and Whey protein concentrate 80 (WPC-80) in people with Healthy Participants, run by Norwegian School of Sport Sciences.
Trial summary
The aim of this study is to investigate the amino acid kinetics in blood after a bout of strength training and ingestion of different milk protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk) The investigators hypothesize that native whey will give a faster… Read more about this study
- Who is it for?
- Ages 18 to 45 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Healthy Participants
- What is being tested?
- Strength Training, Milk 1% and Whey protein concentrate 80 (WPC-80)
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 13 participants
- Where
- No sites listed yet
- Study dates
- Sep 2012to Feb 2013
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 45 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Healthy in the sense that they can conduct training and testing
- Able to understand Norwegian language written and oral
Who cannot join
- Diseases or injuries contraindicating participation
- Use of dietary supplements (e.g. proteins, vitamins and creatine)
- Lactose intolerance
- Allergy to milk
- Allergy towards local anesthetics (xylocain)
About this study
Official title: Does Native Whey Induce Greater Blood Leucine Concentrations Than Other Whey Protein Supplements and Milk: A Randomized Controlled Trial?
The aim of this study is to investigate the amino acid kinetics in blood after a bout of strength training and ingestion of different milk protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk) The investigators hypothesize that native whey will give a faster and higher rise in blood concentrations of leucine compared to the other milk protein supplements.
Read the detailed description
Increasing or maintaining muscle mass is of great importance for populations ranging from athletes to patients and elderly. Resistance exercise and protein ingestion are two of the most potent stimulators of muscle protein synthesis. Both the physical characteristic of proteins (e.g. different digestion rates of whey and casein) and the amino acid composition, affects the potential of a certain protein to stimulate muscle protein synthesis. Given its superior ability to rapidly increase blood leucine concentrations to high levels, whey is often considered the most potent protein source to stimulate muscle protein synthesis. Native whey protein is produced by filtration of unprocessed milk. Consequently, native whey has different characteristics than WPC-80, which is exposed to heating and acidification. Because of the direct filtration of unprocessed milk, native whey is a more intact protein compared with WPC-80. Of special interest is the higher amounts of leucine in native whey.
The aim of this double-blinded randomized 5-arm cross-over study is to compare amino acid kinetics in blood after a bout of strength training and ingestion of 20 grams of high quality, but distinct, dairy protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk). Furthermore, the investigators investigate whether differences in amino acid kinetics affect acute blood glucose and urea response, as well as recovery of muscle function after a bout of strength training.
The investigators hypothesize that native whey will give a faster and higher rise in blood concentrations of leucine compared to the other protein supplements.
- Conditions
- Healthy Participants
- Being tested
- Strength Training, Milk 1%, Whey protein concentrate 80 (WPC-80), Microparticulated whey, Hydrolyzed whey
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Strength TrainingOther
Milk 1%Dietary supplement
Also called Protein supplementation
Whey protein concentrate 80 (WPC-80)Dietary supplement
Also called Protein supplementation
Microparticulated wheyDietary supplement
Also called Protein supplementation
Hydrolyzed wheyDietary supplement
Also called Protein supplementation
Native wheyDietary supplement
Also called Protein supplementation
| Study group | Role | What they receive |
|---|---|---|
| Milk 1% | Placebo comparator | Participants performed a bout of strength training and consumed 636 ml of a protein supplement Receives: Other: Strength Training; Dietary Supplement: Milk 1% |
| Whey protein concentrate 80 (WPC-80) | Experimental | Participants performed a bout of strength training and consumed 636 ml of a protein supplement Receives: Other: Strength Training; Dietary Supplement: Whey protein concentrate 80 (WPC-80) |
| Microparticulated whey | Experimental | Participants performed a bout of strength training and consumed 636 ml of a protein supplement Receives: Other: Strength Training; Dietary Supplement: Microparticulated whey |
| Hydrolyzed whey | Experimental | Participants performed a bout of strength training and consumed 636 ml of a protein supplement Receives: Other: Strength Training; Dietary Supplement: Hydrolyzed whey |
| Native whey | Experimental | Participants performed a bout of strength training and consumed 636 ml of a protein supplement Receives: Other: Strength Training; Dietary Supplement: Native whey |
What is being measured
The primary outcome is the main question the study is designed to answer.
Plasma amino acid concentration change from baseline
Measured: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements
Secondary outcomes (6)
Serum glucose change from baseline
Measured: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements
Serum urea change from baseline
Measured: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements
Muscle force generating capacity change from baseline
Measured as unilateral isometric knee extension force (Nm) with 90° in the hip and knee joints.
Measured: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Jump height change from baseline
Measured as counter movement jump on a force plate
Measured: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Serum creatine kinase change from baseline
Measured: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements
Muscle soreness change from baseline
Measured for m. quadriceps and m. pectoralis major on a visual analog pain scale
Measured: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted29 Aug 2016 (estimated)
- Study startSep 2012
- Primary completionOct 2012
- Study completionFeb 2013
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Healthy ParticipantsStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Quadruple blind
- Who is masked
- Participant, Care provider, Investigator, Outcomes assessor
- Enrolment
- 13 participants (actual)
Sponsor and investigators
- Lead sponsor
- Norwegian School of Sport Sciences
- Sponsor type
- University, hospital or other
- Collaborators
- Arkansas Children's Hospital Research Institute, Tine
- Responsible party
- Principal investigator, Havard Hamarsland, Norwegian School of Sport Sciences
- Investigators
- Truls Raastad, PhD, Principal investigator, Norwegian School of Sport Sciences
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT02882386
- Sponsor's protocol ID
- NorwegianSSS
- Status last verified
- Aug 2016
- First submitted
- 16 Aug 2016
- Last updated
- 29 Aug 2016
- Results posted
- No
- Data monitoring committee
- No
- Individual data sharing
- Undecided
Study at a glance
From the registry record, last updated by the sponsor on 29 Aug 2016. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Does Native Whey Induce Greater Blood Leucine Concentrations Than Other Whey Protein Supplements and Milk: A Randomized Controlled Trial?
- ClinicalTrials.gov ID
- NCT02882386
- Acronym / sponsor ID
- NorwegianSSS
- Conditions
- Healthy Participants
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 13 participants (actual)
- Lead sponsor
- Norwegian School of Sport SciencesUniversity, hospital or other
- Collaborators
- Arkansas Children's Hospital Research Institute, Tine
- Study start
- Sep 2012
- Primary completion
- Oct 2012 (actual)
- Study completion
- Feb 2013 (actual)
- First posted
- 29 Aug 2016 (estimated)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The aim of this study is to investigate the amino acid kinetics in blood after a bout of strength training and ingestion of different milk protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk) The investigators hypothesize that native whey will give a faster and higher rise in blood concentrations of leucine compared to the other milk protein supplements.
Detailed description
Increasing or maintaining muscle mass is of great importance for populations ranging from athletes to patients and elderly. Resistance exercise and protein ingestion are two of the most potent stimulators of muscle protein synthesis. Both the physical characteristic of proteins (e.g. different digestion rates of whey and casein) and the amino acid composition, affects the potential of a certain protein to stimulate muscle protein synthesis. Given its superior ability to rapidly increase blood leucine concentrations to high levels, whey is often considered the most potent protein source to stimulate muscle protein synthesis. Native whey protein is produced by filtration of unprocessed milk. Consequently, native whey has different characteristics than WPC-80, which is exposed to heating and acidification. Because of the direct filtration of unprocessed milk, native whey is a more intact protein compared with WPC-80. Of special interest is the higher amounts of leucine in native whey.
The aim of this double-blinded randomized 5-arm cross-over study is to compare amino acid kinetics in blood after a bout of strength training and ingestion of 20 grams of high quality, but distinct, dairy protein supplements (native whey, whey protein concentrate 80, hydrolysed whey, microparticulated whey and milk). Furthermore, the investigators investigate whether differences in amino acid kinetics affect acute blood glucose and urea response, as well as recovery of muscle function after a bout of strength training.
The investigators hypothesize that native whey will give a faster and higher rise in blood concentrations of leucine compared to the other protein supplements.
Study design
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Quadruple blind (participant, care provider, investigator, outcomes assessor)
- Number of arms
- 5
| Arm | Type | Receives |
|---|---|---|
| Milk 1% | Placebo comparator | Other: Strength Training, Dietary Supplement: Milk 1%Participants performed a bout of strength training and consumed 636 ml of a protein supplement |
| Whey protein concentrate 80 (WPC-80) | Experimental | Other: Strength Training, Dietary Supplement: Whey protein concentrate 80 (WPC-80)Participants performed a bout of strength training and consumed 636 ml of a protein supplement |
| Microparticulated whey | Experimental | Other: Strength Training, Dietary Supplement: Microparticulated wheyParticipants performed a bout of strength training and consumed 636 ml of a protein supplement |
| Hydrolyzed whey | Experimental | Other: Strength Training, Dietary Supplement: Hydrolyzed wheyParticipants performed a bout of strength training and consumed 636 ml of a protein supplement |
| Native whey | Experimental | Other: Strength Training, Dietary Supplement: Native wheyParticipants performed a bout of strength training and consumed 636 ml of a protein supplement |
Who may qualify
- Ages
- 18 to 45 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (2 listed)
- Healthy in the sense that they can conduct training and testing
- Able to understand Norwegian language written and oral
Key exclusion criteria (5 listed)
- Diseases or injuries contraindicating participation
- Use of dietary supplements (e.g. proteins, vitamins and creatine)
- Lactose intolerance
- Allergy to milk
- Allergy towards local anesthetics (xylocain)
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Strength Training | Other | Hydrolyzed whey, Microparticulated whey, Milk 1%, Native whey, Whey protein concentrate 80 (WPC-80) |
| Milk 1%Also: Protein supplementation | Dietary supplement | Milk 1% |
| Whey protein concentrate 80 (WPC-80)Also: Protein supplementation | Dietary supplement | Whey protein concentrate 80 (WPC-80) |
| Microparticulated wheyAlso: Protein supplementation | Dietary supplement | Microparticulated whey |
| Hydrolyzed wheyAlso: Protein supplementation | Dietary supplement | Hydrolyzed whey |
| Native wheyAlso: Protein supplementation | Dietary supplement | Native whey |
Primary outcome
Plasma amino acid concentration change from baseline
Time frame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements
6 secondary outcomes
Serum glucose change from baseline
Time frame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements
Serum urea change from baseline
Time frame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements
Muscle force generating capacity change from baseline
Time frame: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Jump height change from baseline
Time frame: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Serum creatine kinase change from baseline
Time frame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements. For milk and native whey blood was collected at two additional time points: 22 and 30 hours after consumption of protein supplements
Muscle soreness change from baseline
Time frame: Measured before and at 0, 6, 22 and 30 hours after exercise. Only measured after milk and native whey
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 29 Aug 2016.
- Individual participant dataSharing planned: Undecided
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Truls Raastad, PhD principal investigator
Norwegian School of Sport Sciences
Questions about this trial
What is this clinical trial studying?
A study testing Strength Training, Milk 1% and Whey protein concentrate 80 (WPC-80) in people with Healthy Participants, run by Norwegian School of Sport Sciences.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 2 inclusion and 5 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 29 Aug 2016. Status can change between updates, so confirm with the study contact before making plans.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Plasma amino acid concentration change from baseline (time frame: Blood collected at 0, 30, 45, 60, 90 and 120 min after consumption of protein supplements). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 29 Aug 2016; AzTrials last refreshed its copy on 7 Nov 2026.