Biology, Identity & Opportunity Study

The investigators will assess student emotional wellbeing, specifically student self-esteem using the Rosenberg Self-esteem Scale (Rosenberg, 1979; Phinney et al., 1997; Umaña-Taylor \& Updegraff, 2007). The scale includes 10 items on a 1 (strongly disagree) through 5 (strongly agree) Likert-type scale. Five negatively worded items are reversed scored so that higher scores indicate higher levels of self-esteem. The mean score is computed for the scale. Therefore the minimum score is 1, and maximum is 5.

The investigators will assess student emotional wellbeing, specifically student symptoms of depression using the Center for Epidemiological Studies Depression Scale (CES-D; Hughes et al., 2004; Radloff, 1977). The CES-D includes 20 items on a 1 (rarely or none of the time) to 4 (most of the time) Likert-type scale. The CES-D consists of four subscales (Somatic Symptoms, Negative Affect, Positive Affect, Interpersonal Problems). Negatively worded items are reverse scored for the overall score so that higher scores indicate higher levels of depressive symptoms, but are not reverse scored for the positive affect subscale. A mean of the 20 items is calculated to obtain the total depressive symptoms score, therefore the minimum score is 1, and maximum is 4.

The investigators will assess student emotional wellbeing, specifically student symptoms of depression (somatic symptoms) using the Center for Epidemiological Studies Depression Scale (CES-D; Hughes et al., 2004; Radloff, 1977). The CES-D includes 20 items on a 1 (rarely or none of the time) to 4 (most of the time) Likert-type scale. 7 items compose the Somatic Symptoms subscale. Negatively worded items are reverse scored for the somatic symptoms subscale score, so that higher scores indicate higher levels of somatic symptoms. A mean of the 7 somatic symptoms items is calculated to obtain the Somatic Symptoms subscale score, therefore the minimum score is 1, and maximum is 4.

The investigators will assess student emotional wellbeing, specifically student symptoms of depression (negative affect) using the Center for Epidemiological Studies Depression Scale (CES-D; Hughes et al., 2004; Radloff, 1977). The CES-D includes 20 items on a 1 (rarely or none of the time) to 4 (most of the time) Likert-type scale. 7 items compose the Negative Affect subscale. Negatively worded items are reverse scored for the Negative Affect subscale, so that higher scores indicate higher levels of negative affect. A mean of the 7 negative affect symptoms items is calculated to obtain the Negative Affect subscale score, therefore the minimum score is 1, and maximum is 4.

The investigators will assess student emotional wellbeing, specifically student symptoms of depression (positive affect) using the Center for Epidemiological Studies Depression Scale (CES-D; Hughes et al., 2004; Radloff, 1977). The CES-D includes 20 items on a 1 (rarely or none of the time) to 4 (most of the time) Likert-type scale. 4 items compose the Positive Affect subscale. Negatively worded items are NOT reverse scored for the Positive Affect subscale, so that higher scores indicate higher levels of positive affect. A mean of the 4 positive affect symptoms items is calculated to obtain the Positive Affect subscale score, therefore the minimum score is 1, and maximum is 4.

The investigators will assess student emotional wellbeing, specifically student symptoms of depression (interpersonal problems) using the Center for Epidemiological Studies Depression Scale (CES-D; Hughes et al., 2004; Radloff, 1977). The CES-D includes 20 items on a 1 (rarely or none of the time) to 4 (most of the time) Likert-type scale. 2 items compose the Interpersonal Problems subscale. Negatively worded items are reverse scored for the overall score so that higher scores indicate higher levels of interpersonal problems. A mean of the 2 interpersonal problems items is calculated to obtain the Interpersonal Problems subscale score, therefore the minimum score is 1, and maximum is 4.

The investigators will assess student emotional wellbeing, specifically adolescent internalizing and externalizing symptoms using Child Behavior Check List, Youth Self Report 11-18 (CBCL YSR; Achenbach, 1991). The YSR includes 112 problem items on a 1 (not true) to 3 (very true or often true) Likert-type scale. The total problem scale is divided into 9 syndrome subscales (i.e., withdrawn, somatic complaints, anxious/depressed, social problems, thought problems, attention problems, delinquent behavior, aggressive behavior, and self-destructive/identity problems). The items are summed for a raw score, which is converted to a T-score and corresponding percentile. A T-Score of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and is indicative of clinically significant adolescent problems (both internalizing and externalizing). Higher scores indicate worse symptoms.

The investigators will assess student emotional wellbeing, specifically adolescent externalizing symptoms using Child Behavior Check List, Youth Self Report 11-18 (CBCL YSR; Achenbach, 1991). The YSR includes 112 problem items on a 0 (not true) to 2 (very true or often true) Likert-type scale. The externalizing problem scale can be divided into 6 syndrome subscales (i.e., social problems, thought problems, attention problems, delinquent behavior, aggressive behavior, and self-destructive/identity problems). The externalizing problems from the 6 subscales are summed for a raw score, which is converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant externalizing problems. Higher scores are indicative of worse symptoms.

The investigators will assess student emotional wellbeing, specifically adolescent internalizing symptoms using Child Behavior Check List, Youth Self Report 11-18 (CBCL YSR; Achenbach, 1991). The YSR includes 112 problem items on a 1 (not true) to 3 (very true or often true) Likert-type scale. The internalizing problem scale can be divided into 3 syndrome subscales (i.e., withdrawn, somatic complaints, and anxious/depressed). The internalizing problems from the 3 subscales are summed for a raw score, which is converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant internalizing problems. Higher scores are indicative of worse symptoms.

The investigators will assess academic engagement using the Academic Engagement Scale (Skinner, Kindermann, \& Furrer, 2008). The scale contains 10 items on a 1 (never) to 5 (all of the time) Likert-type scale. No items need to be reverse scored. Higher values indicate more engagement with school. The mean score for the 10 items is used to calculate the overall academic engagement score, with a minimum score of 1, and a maximum of 5.

The investigators will assess a component of academic engagement, namely, behavioral engagement, from the Behavioral Engagement subscale of the Academic Engagement Scale (Skinner, Kindermann, \& Furrer, 2008). The scale contains 10 items on a 1 (never) to 5 (all of the time) Likert-type scale, and 6 items comprise the Behavioral Engagement subscale. No items need to be reverse scored. Higher values indicate more behavioral engagement with school. The mean score for the 6 items is used to calculate the behavioral engagement score, with a minimum score of 1, and a maximum of 5.

The investigators will assess school belonging, from the School Belonging Scale (McNeely, Nonnemaker, \& Blum, 2002; Smalls, 2010; Smalls \& Cooper, 2012). The scale contains 5 items on a 1 (strongly disagree) to 5 (strongly agree) Likert-type scale. There are no subscales in this measure. No items need to be reverse scored. Higher values reflect greater connectedness. The mean score for the 5 items is used to calculate the school belonging score, with a minimum score of 1, and a maximum of 5.

The investigators will assess academic motivation, from the Academic Motivation Scale (Plunkett \& Bámaca-Gómez, 2003). The scale contains 5 items on a 1 (strongly disagree) to 4 (strongly agree) Likert-type scale. There are no subscales in this measure. No items need to be reverse scored. Higher values reflect more academic motivation. The mean score for the 5 items is used to calculate the academic motivation score, with a minimum score of 1, and a maximum of 4.

The investigators will assess academic efficacy, from the Academic Efficacy Scale (Alfaro et al., 2006; Alfaro et al., 2009; Midgley et al., 2000). The scale contains 5 items on a 1 (not at all true) to 5 (very true) Likert-type scale. There are no subscales in this measure. No items need to be reverse scored. Higher values reflect more academic efficacy. The mean score for the 5 items is used to calculate the academic efficacy score, with a minimum score of 1, and a maximum of 5.

The investigators will assess achievement and attainment by collecting standardized achievement tests scores, including the STAR reading and math scores.

The investigators will assess achievement and attainment by collecting student report and administrative reports on grades and collecting end of year GPA scores.

The investigators will assess self-reported executive functioning using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The overall score for all 80 items creates the Global Executive Composite to assess an adolescent's view of his or her cognitive, emotional, and behavioral functions. The 80 items are summed for a raw score, which is converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning. Higher scores are indicative of worse executive functioning.

The investigators will assess self-reported executive functioning using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The Behavioral Regulation Index is comprised of 3 scales (i.e., inhibit, shift, emotional control). The items from these 3 scales are summed for a raw score, which is converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning, specially related to behavioral regulation. Higher scores are indicative of worse executive functioning.

The investigators will assess self-reported executive functioning using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The Metacognition Index is comprised of 5 scales (i.e., initiate, working memory, plan/organize, organization of materials, and monitor). The items from these 5 scales are summed for a raw score, which is converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning, specially related to metacognition. Higher scores are indicative of worse executive functioning, namely metacognition.

The investigators will assess self-reported executive functioning (inhibition) using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The Inhibit Scale is one of three scales that make up the Behavioral Regulation Index. The items from this scale are summed for a raw score, which are converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning, specially related to inhibition. Higher scores are indicative of worse executive functioning, namely poorer ability to control impulses (inhibitory control) and to stop engaging in a behavior.

The investigators will assess self-reported executive functioning (Shifting) using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The Shift Scale is one of three scales that make up the Behavioral Regulation Index. The items from this scale are summed for a raw score, which are converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning, specially related to shifting. Higher scores are indicative of worse executive functioning, namely poorer ability to move freely from one activity or situation to another; to tolerate change; to switch or alternate attention.

The investigators will assess self-reported executive functioning (Emotional Control) using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The Emotional Control Scale is one of three scales that make up the Behavioral Regulation Index. The items from this scale are summed for a raw score, which are converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning, specially related to emotional control. Higher scores are indicative of worse executive functioning, namely poorer ability to regulate emotional responses appropriately.

The investigators will assess self-reported executive functioning (Initiation) using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The Initiate Scale is one of five scales that make up the Metacognition Index. The items from this scale are summed for a raw score, which are converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning, specially related to initiation. Higher scores are indicative of worse executive functioning, namely poorer ability to begin an activity and to independently generate ideas or problem-solving strategies.

The investigators will assess self-reported executive functioning (Working Memory) using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The Working Memory Scale is one of five scales that make up the Metacognition Index. The items from this scale are summed for a raw score, which are converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning, specially related to working memory. Higher scores are indicative of worse executive functioning, namely poorer ability to hold information when completing a task, when encoding information, or when generating goals/plans in a sequential manner.

The investigators will assess self-reported executive functioning (Planning/Organization) using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The Plan/Organize Scale is one of five scales that make up the Metacognition Index. The items from this scale are summed for a raw score, which are converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning, specially related to planning/organization. Higher scores are indicative of worse executive functioning, namely poorer ability to anticipate future events; to set goals; to develop steps; to grasp main ideas; to organize and understand the main points in written or verbal presentations.

The investigators will assess self-reported executive functioning (Organization of Materials) using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The Organization of Materials Scale is one of five scales that make up the Metacognition Index. The items from this scale are summed for a raw score, which are converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning, specially related to organization of materials. Higher scores are indicative of worse executive functioning, namely poorer ability to put order in work, play and storage spaces (e.g., desks, lockers, backpacks, and bedrooms).

The investigators will assess self-reported executive functioning (Self-Monitoring) using the Behavior Rating Inventory of executive function Self-Report Version (Guy, Gioia, \& Isquith, 2004). The BRIEF contains 80 items on a 1 (never a problem) to 3 (often a problem) Likert-type scale. The Monitor Scale is one of five scales that make up the Metacognition Index. The items from this scale are summed for a raw score, which are converted to a T-score and corresponding percentile. T of 65 or greater represents 1.5 standard deviations (or greater) above the mean, and indicative of clinically significant deficits in executive functioning, specially related to self-monitoring. Higher scores are indicative of worse executive functioning, namely poorer ability to check work and to assess one's own performance; ability to keep track of the effect of one's own behavior on other people.

A brief paper and pencil measure of executive functioning, the Trail Making Test Part A (TMT-A) and B (TMT-B), will be administered (Tombaugh, 2004). TMT-B provides a measure of cognitive flexibility and set switching. The total raw score time for TMT-B is used in combination with demographic information (e.g., age, gender, education, race/ethnicity), to look up corresponding T-Scores in Heaton norms. The normal bell curve is used to evaluate T-scores (e.g., T-scores between 43 and 57 falling in the average range), with lower scores indicative of poorer executive functioning.

A brief paper and pencil measure of executive functioning, the Trail Making Test Part A (TMT-A) and B (TMT-B), will be administered (Tombaugh, 2004). TMT-B provides a measure of cognitive flexibility and set switching. The total number of errors (i.e., set-shifting and sequencing errors) for TMT-B are summed, with higher scores indicative of poorer executive functioning. The range is dependent on the number of errors made.

A brief paper and pencil measure of executive functioning, the Trail Making Test Part A (TMT-A) and B (TMT-B), will be administered (Tombaugh, 2004). TMT-B provides a measure of cognitive flexibility and set switching. The total number of set-shifting errors (i.e., not correcting shifting between letters and numbers) for TMT-B are summed, with higher scores indicative of poorer executive functioning. The range is dependent on the number of errors made.

A brief paper and pencil measure of executive functioning, the Trail Making Test Part A (TMT-A) and B (TMT-B), will be administered (Tombaugh, 2004). TMT-B provides a measure of cognitive flexibility and set switching. The total number of sequencing errors for TMT-B are summed, with higher scores indicative of poorer executive functioning. The range is dependent on the number of errors made.

A brief paper and pencil measure of executive functioning, the Trail Making Test Part A (TMT-A) and B (TMT-B), will be administered (Tombaugh, 2004). TMT-B provides a measure of cognitive flexibility and set switching. The total raw score time for TMT-A is subtracted from TMT-B to obtain a difference score, to remove the effects of pure processing speed from the TMT-B executive functioning scores. Higher scores are indicative of more time to complete the TMT-B task (while removing baseline processing speed), and suggestive of worse executive functioning. There are no recommended cut-offs, and this score is rather a comparative score.

A brief paper and pencil measure of executive functioning, the Trail Making Test Part A (TMT-A) and B (TMT-B), will be administered (Tombaugh, 2004). TMT-A provides a measure of speed of processing. The total raw score time for TMT-A is used in combination with demographic information (e.g., age, gender, education, race/ethnicity), to look up corresponding T-Scores in Heaton norms. The normal bell curve is used to evaluate T-scores (e.g., T-scores between 43 and 57 falling in the average range), with lower scores indicative of poorer executive functioning, namely related to processing speed.

The NIH Toolbox Dimensional Change Card Sort Test (Gershon et al., 2013; Zelazo, \& Bauer, 2013) measures set shifting, or the capacity for switching among multiple aspects of a strategy or task. Scoring is based on a combination of accuracy and reaction time. A 2-vector scoring method is employed that uses accuracy and reaction time, where each of these "vectors" ranges in value between 0 and 5, and the computed score, combining each vector score, ranges in value from 0-10. For any given individual, accuracy is considered first. If accuracy levels for the participant are less than or equal to 80%, the final "total" computed score is equal to the accuracy score. If accuracy levels for the participant reach more than 80%, the reaction time score and accuracy score are combined.

The NIH Toolbox Flanker Inhibitory Control and Attention Test (Gershon et al., 2013; Zelazo, \& Bauer, 2013) measures the allocation of one's limited capacities to deal with an abundance of environmental stimulation. Scoring is based on a combination of accuracy and reaction time and is identical for both the Flanker and DCCS measures (described below). A 2-vector scoring method is employed that uses accuracy and reaction time, where each of these "vectors" ranges in value between 0 and 5, and the computed score, combining each vector score, ranges in value from 0-10. For any given individual, accuracy is considered first. If accuracy levels for the participant are less than or equal to 80%, the final "total" computed score is equal to the accuracy score. If accuracy levels for the participant reach more than 80%, the reaction time score and accuracy score are combined.

The NIH Toolbox Picture Sequence Memory Test (Gershon et al., 2013; Zelazo, \& Bauer, 2013) measures the acquisition, storage and retrieval of new information. It involves conscious recollection of information learned within a context. The PSMT is scored using IRT methodology. The number of adjacent pairs placed correctly for each of trials 1 and 2 is converted to a theta score, which provides a representation of the given participant's estimated ability in this episodic memory task. All normative standard scores are provided. Scores are converted to age-corrected Standard Scores and fully-corrected (including gender and educational level) T-Scores. The present study will use fully-corrected T-scores, which are evaluated on the normal bell curve.

The NIH Toolbox Pattern Comparison Processing Speed Test (Gershon et al., 2013; Zelazo, \& Bauer, 2013) measures the amount of time it takes to mentally process a set amount of information, or the amount of information that can be processed within a certain unit of time. It is a measure that reflects mental efficiency. The participant's raw score is the number of items answered correctly in 85 seconds of response time, with a range of 0-130. This score is then converted to the NIH Toolbox normative standard scores. Scores are converted to age-corrected Standard Scores and fully-corrected (including gender and educational level) T-Scores. The present study will use fully-corrected T-scores, which are evaluated on the normal bell curve.

The NIH Toolbox List Sorting Working Memory Test (Gershon et al., 2013; Zelazo, \& Bauer, 2013) measures the capacity of an individual to hold information in a short-term buffer and manipulate the information. List Sorting is scored by summing the total number of items correctly recalled and sequenced on 1-List and 2-List, which can range from 0-26. This score is then converted to the nationally normed standard scores. Scores are converted to age-corrected Standard Scores and fully-corrected (including gender and educational level) T-Scores. The present study will use fully-corrected T-scores, which are evaluated on the normal bell curve.

The NIH Toolbox computes a Fluid Intelligence composite score (Gershon et al., 2013; Zelazo, \& Bauer, 2013). This composite includes the Flanker, Dimensional Change Card Sort, Picture Sequence Memory, List Sorting and Pattern Comparison. The composite score is derived by averaging the standard scores of each of the measures, and then deriving standard scores based on this new distribution. An Age-Corrected Standard Score, Fully Corrected T-Score, Uncorrected Standard Score and associated Percentiles are computed. Higher scores indicate higher levels of functioning. An uncorrected or age-corrected standard score at or near 100 indicates ability that is average compared with others nationally. A Fully Corrected T-Score at or near 50 indicates ability that is average compared with others nationally and with similar demographic characteristics, and one below 40 suggests the possibility of health-related, acquired cognitive impairment.

An objective measure of hours of sleep will be determined by a research-grade actigraph, a device word on the wrist that provides an ongoing activity record that can be scored for intervals of sleeping and waking, and provide an objective estimate of total numbers of hours spent sleeping. The Sadeh scoring algorithm will be used, and hours of sleep will be examined on a scale of number of hours.

An objective measure of sleep quality will be determined by actigraphy, a wristwatch-like device that provides an ongoing activity record that can be scored to provide estimates of sleep efficiency -- the proportion of time spent sleeping during the time between the individual attempted to fall asleep and their final morning awakening. Higher sleep efficiency is a reflection of better sleep quality, and is measured as a proportion or percent (on a scale from 0 to 100).

An objective measure of sleep latency will be determined by actigraphy, a wristwatch-like device that provides an ongoing activity record that can be scored to provide estimates of sleep latency, which is the number of minutes it take from the time the individual goes to bed and when they fall asleep. Measured in number of minutes, with more minutes representing a longer sleep latency.

Salivary cortisol samples self-collected by participants four times per day for four weekdays (Monday through Thursday) on the same week as the diary and sleep measurement, at waking, 30 minutes after waking, immediately after school, and at bedtime. The diurnal cortisol slope is rate of change (usually decline) in cortisol from waking to bedtime, measured in micrograms per deciliter per hour. A steeper rate of decline in cortisol from waking to bedtime is considered an indicator of positive cortisol functioning; the researchers expect a stronger decline in cortisol in the experimental as compared to the active comparison condition. The researchers expect this to be the strongest cortisol finding, with larger effect sizes than for the next two cortisol outcome variables.

Salivary cortisol samples self-collected by participants four times per day for four weekdays (Monday through Thursday) on the same week as the diary and sleep measurement, at waking, 30 minutes after waking, immediately after school, and at bedtime. The cortisol awakening response is the difference the 30 minutes post-awakening sample and the waking sample (30 minute sample - waking sample), averaged across days. The researchers expect to see a smaller cortisol awakening response among individuals in the experimental ERI intervention condition as compared to the control (active comparator) condition.

Salivary cortisol samples self-collected by participants four times per day for four weekdays (Monday through Thursday) on the same week as the diary and sleep measurement, at waking, 30 minutes after waking, immediately after school, and at bedtime. The average cortisol level is the area under the curve of the cortisol data points each day, divided by the total time awake that day, and averaged across the four days. This outcome will be standardized for ease of interpretation. The researchers do not expect to find significant intervention vs. control differences for this variable.