Immunogenicity of Fractional One-fifth and One-half Doses of Yellow Fever Vaccine Compared to Full Dose in Children 9-23 Months Old

Yellow fever (YF) is a potentially fatal disease causing some 29,000 - 60,000 deaths in Africa alone in 2013. Vaccination with the YF vaccine is the most effective way of preventing YF and a single full dose of vaccine provides life-long protection. Indeed, over half a billion doses of the YF vaccine have been successfully used worldwide since its introduction over 80 years ago in the 1930s, including in children <2 years of age. The World Health Organization (WHO) recommends that YF vaccine be administered to all people ≥ 9 months of age. It should be noted that four YF vaccines are prequalified for use by WHO. Between 2007 and 2016, more than 105 million people have been vaccinated against YF in 14 African countries, including millions of children 9-24 months of age. Each year 6 million doses of YF vaccine are placed into an emergency stockpile to assist in the rapid response and control of outbreaks. During 2016, large outbreaks of YF in Angola and the Democratic Republic of Congo (DRC) depleted the stockpile multiple times and led to critical shortages in YF vaccine. Given the acute vaccine shortage, the World Health Organization (WHO) reviewed available data and determined that the use of fractional dose YF vaccination was a safe and effective option for mass vaccination to control outbreaks. Fractional 1/5th dose of YF vaccine manufactured by BioManguinhos, 17DD, was then used in a campaign targeting 8 million people in DRC's capital city of Kinshasa. However, only persons aged 2 years of age and older were given the fractional dose as there were no data regarding its use in children. To optimize vaccination coverage and vaccine use in outbreak responses, studies of fractional dose YF vaccine effectiveness in children less than 2 years of age were assessed as a research priority by the Strategic Advisory Group of Experts on Immunization (SAGE).

To address this critical information need, the investigators will conduct a randomized clinical trial comparing fractional dose 17DD YF vaccination to the full dose among children aged 9-23 months in Uganda. Exclusion criteria will include children who have previously received YF vaccine or who have contraindications or precautions to YF vaccination.

Enrolled participants will be randomized to one of three arms:

1. One-fifth fractional dose (0.1 ml) administered subcutaneously B. One-half fractional dose (0.25 ml) administered subcutaneously C. Full dose (0.5 ml) administered subcutaneously

The one-fifth and one-half doses of YF vaccine were both recommended for further research by WHO and YF vaccine experts.

Three large public health centers that provide vaccination through the Ministry of Health of Uganda's Expanded Programme on Immunization will be selected in Kampala and nearby districts as study sites. Blood samples (3 ml) will be collected from participants before vaccine administration as well as at follow-up visits 4 weeks and 12 months after vaccination. Presence of YF virus-specific neutralizing antibodies in the collected specimens will be determined using the plaque reduction neutralization test (PRNT). Seroconversion will be defined as seronegative participants (<1:10 titers) at enrollment who become seropositive (≥1:10) at 4 week follow-up. An immune response will be defined as either participants that seroconvert or participants who demonstrate a four-fold or greater change in titers between the baseline and 4 week specimens, e.g. a change from 1:20 to 1:80. The proportion of children seroconverting in each of the fractional dose arms will be compared to the full dose arm using a non-inferiority test. The safety profile also will be described for both solicited and unsolicited adverse events following immunization (AEFIs).

The investigators will collect data on all illnesses encountered by study participants during the first 28 days post-vaccination to assess potential AEFI as recommended by the WHO. Since almost all AEFI occur during the first 2 weeks post-vaccination, parents/guardians will be interviewed at 2 weeks to determine if there are any signs and symptoms that could be consistent with a serious AEFI known to be associated with YF vaccination. In addition, parents will be asked to take their child's temperature twice during the day and complete a structured diary card to document all of their child's signs and symptoms for pre-specified and un-specified adverse events during the first 2 weeks. This will be reviewed by study staff at the 2 week visit as described above. At the 4 week visit, parents/guardians will be asked about illnesses during the interval between their 2 week and 4 week visits as described above. In addition, parents will be reminded and instructed at all interactions during the first 28 days after vaccination to call study staff anytime on the 72-hour study phone line and bring their child directly to the study hospital if they develop any condition(s) requiring medical attention during the first 28 days after vaccination. Any adverse events observed will be reported to the national reporting system.