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ExerciseHIIRTNCT04321772

Muscle Response to High Intensity Interval Resistance Training (HIIRT)

A study testing High Intensity Interval Resistance Training and Traditional Resistance Training in people with Exercise and Resistance Training, run by University of Padova at 1 site in Italy.

Completed

Finished. The study has ended and is not enrolling. Results may be posted on the official record.

Official record
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Trial summary

Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. Read more about this study

Who is it for?
Ages 20 to 30 yearsHealthy volunteers can take part
Conditions the study is for
Exercise, Resistance Training
What is being tested?
High Intensity Interval Resistance Training and Traditional Resistance Training
Could I receive a placebo?
No, everyone receives an active treatment
How many people
9 participants
Where
1 siteItaly
Study dates
1 Feb 2012to 10 Feb 2013
Phase
Interventional
Are there results?
None posted in the registry

Key requirements

Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.

  • Ages 20 to 30 years
  • All sexes
  • Healthy volunteers accepted
  • Adult

Who may be able to join

  • moderate active
  • experience with resistance training

Who cannot join

  • history of recent myocardial infarction,
  • severe cardiac arrhythmia,
  • unstable angina,
  • poorly controlled hypertension,
  • poorly controlled diabetes mellitus,
  • frequent or complex ventricular ectopy which might interfere with one's ability to adhere to exercise protocols

Trial locations and contacts

1 site in 1 country.

Italy1 site

  • Nutrition and Exercise Lab, DSB, University of Padova

    Padova, 35131

Other trials in Italy

About this study

Official title: Effects of Exercise Intensity and Rest Intervals on Intracellular Signals and Anabolic Response of Skeletal Muscle to Resistance Training

Resistance training (RT) is one of the most important stimuli for muscle hypertrophy and it plays an important role on weight loss and fatty acid oxidation increase. RT affects anabolic pathways but the differences among various training techniques has been till now were poorly investigated. The aim of this study was to compare the effect of two different intensity of training, high-intensity interval resistance training (HIIRT) and traditional resistance training (TRT), on muscle signalling pathway.

Read the detailed description

Nine young healthy subjects performed HIIRT and TRT protocol in two different moments and with different legs. HIIRT technique consisted of three sets of: 6 repetitions at 6RM (6 repetitions maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets. Biopsies from the vastus lateralis were taken at baseline (pre), immediately (0h) at the end of training, 6 hours (6h) and 24 hours (24h) after training. Western blot and RT-PCR mRNA (Reverse transcriptase-polymerase chain reaction) analysis were performed to assess muscle signalling pathway activation

Being tested
High Intensity Interval Resistance Training, Traditional Resistance Training
Keywords
resistance training, recovery, muscle hypertrophy, muscle signalling
Medical subject headings
Motor Activity

What participants receive

What participants receive. In a randomised study each person is assigned to one group by chance.

  • High Intensity Interval Resistance TrainingOther

    Subjects trained one leg with HIIRT

  • Traditional Resistance TrainingOther

    Subjects trained the other leg with HIIRT

Study groups in NCT04321772
Study groupRoleWhat they receive
High Intensity Interval Resistance Training (HIIRT)ExperimentalHIIRT technique consisted of three sets of: 6 repetitions at 80% 1RM (1 repetition maximum) and then 20 seconds of rest and 2/3 repetitions (until exhaustion) repeated for 3 times with 2'30" rest between sets; while TRT consisted of 3 sets of 15 reps with 75 sec of rest between sets.
Receives: Other: High Intensity Interval Resistance Training
Traditional Resistance Training (TRT)Active comparatorTRT protocol performed 3 series of 15 repetitions at 60% 1RM with 75" of rest between sets.
Receives: Other: Traditional Resistance Training

What is being measured

The primary outcome is the main question the study is designed to answer.

  1. pAMPKThr172 in skeletal muscle tissue measured by immunoblot

    measurement of pAMPKThr172 in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  2. pERK 1/2Thr202/Tyr204 in skeletal muscle tissue measured by immunoblot

    measurement of pERK 1/2Thr202/Tyr204 in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  3. pS6Ser235/236 in skeletal muscle tissue measured by immunoblot

    measurement of pS6Ser235/236 in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  4. p4EBP1Thr37/46 in skeletal muscle tissue measured by immunoblot

    measurement of p4EBP1Thr37/46 in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  5. ACC (acetyl-coenzyme A carboxylase) phosphorylation in skeletal muscle tissue measured by immunoblot

    ACC phosphorylation in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  6. IGF-1 mRNA (messenger ribonucleic acid) in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    IGF-1 mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  7. IGF-1ea (insulin like growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    IGF-1ea mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hoursn

  8. MGF (mechanical growth factor) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    MGF mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  9. STARS mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    STARS mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hoursn

  10. Myogenin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Myogenin mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  11. IL-6 (interleukin 6) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    IL-6 mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  12. PGC1a4 (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha 4) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    PGC1a4 mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  13. PGC1a (Peroxisome proliferator-activated receptor gamma coactivator 1-alpha) mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    PGC1a mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  14. Atrogin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Atrogin mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  15. Beclin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Beclin mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

  16. Myostatin mRNA in skeletal muscle tissue measured by Quantitative polymerase chain reaction (qPCR)

    Myostatin mRNA in skeletal muscle tissue taken by biopsy

    Measured: 24 hours

Timeline

From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.

  1. First posted25 Mar 2020
  2. Study start1 Feb 2012
  3. Primary completion15 May 2012
  4. Study completion10 Feb 2013

Resources

More details

For doctors and researchers: how the study is designed, who runs it, and its registry IDs.

Study design
Study type
Interventional
Phase
Phase N/A
Primary purpose
Basic science
Allocation
Randomised
Intervention model
Crossover
Model description
Same subjects serve as both the test and control arms which allows for within-subject comparison. Using a within-person design, participants trained each lower limb on separate days under two different training stimulus (HIIRT) and traditional training (TRT)
Masking
Single blind
Who is masked
Outcomes assessor
Enrolment
9 participants (actual)
IDs, dates and publications
ClinicalTrials.gov ID
NCT04321772
Sponsor's protocol ID
HIIRT12
Acronym
HIIRT
Status last verified
Mar 2020
First submitted
19 Mar 2020
Last updated
25 Mar 2020
Results posted
No
Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
Individual data sharing
No

Publications

  • Paoli A. Resistance training: the multifaceted side of exercise. Am J Physiol Endocrinol Metab. 2012 Feb 1;302(3):E387. doi: 10.1152/ajpendo.00541.2011. No abstract available.

    PubMed 22275762

  • Paoli A, Moro T, Marcolin G, Neri M, Bianco A, Palma A, Grimaldi K. High-Intensity Interval Resistance Training (HIRT) influences resting energy expenditure and respiratory ratio in non-dieting individuals. J Transl Med. 2012 Nov 24;10:237. doi: 10.1186/1479-5876-10-237.

    PubMed 23176325

  • Paoli A, Pacelli QF, Cancellara P, Toniolo L, Moro T, Canato M, Miotti D, Reggiani C. Myosin isoforms and contractile properties of single fibers of human Latissimus Dorsi muscle. Biomed Res Int. 2013;2013:249398. doi: 10.1155/2013/249398. Epub 2013 Jul 22.

    PubMed 23971027

Questions about this trial

What is this clinical trial studying?

A study testing High Intensity Interval Resistance Training and Traditional Resistance Training in people with Exercise and Resistance Training, run by University of Padova at 1 site in Italy.

The full registered description is in the About this study section and the official record.

Who may be eligible to take part?

The sponsor lists 2 inclusion and 6 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.

Is this clinical trial currently recruiting?

The record says: finished, as of the sponsor's last update on 25 Mar 2020. Status can change between updates, so confirm with the study contact before making plans.

Where is the study taking place?

The record lists 1 site in Italy. Whether a site is taking part right now should be confirmed with that site. See every site.

What are the possible benefits and risks?

They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.

Are results available for this study?

The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.

How can a doctor refer a patient or contact the research team?

The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.

What does the primary outcome measure?

pAMPKThr172 in skeletal muscle tissue measured by immunoblot (time frame: 24 hours). The study lists 16 primary outcomes in all. It is what the study was designed to measure, not a finding.

Where can I read the original study information?

On the ClinicalTrials.gov record for NCT04321772, which also lists 3 linked publications.

Does a listing mean the treatment is approved or proven to work?

No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.

How often is this information updated?

AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 25 Mar 2020.

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