Metabolism in Adipose TissueNCT04566354
Interleukin-6 Secretion in Adipose Tissue Following Sprint Exercise
A study testing Repeated 30-s sprint exercise in people with Metabolism in Adipose Tissue, run by Karolinska Institutet at 1 site in Sweden.
Trial summary
Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Read more about this study
- Who is it for?
- Ages 20 to 40 yearsHealthy volunteers can take part
- Conditions the study is for
- Metabolism in Adipose Tissue
- What is being tested?
- Repeated 30-s sprint exercise
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 18 participants
- Where
- 1 siteSweden
- Study dates
- Sep 2015to Sep 2017
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 20 to 40 years
- All sexes
- Healthy volunteers accepted
- Adult
Who may be able to join
- Physical active
- Healthy
Who cannot join
- Smokers
- Pregnant
- On medication
- No infection
- No asthma
Trial locations and contacts
1 site in 1 country.
Sweden1 site
Karolinska Institutet/Karolinska University Hospital
Stockholm, 141 86
About this study
Official title: Metabolism in Adipose Tissue Followed Following Sprint Exercise
Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.
Read the detailed description
PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators examined acute short-term effects on adipose tissue IL-6 exchange.
METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20 min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery and a superficial subcutaneous vein on the anterior abdominal wall providing access to the venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min and 120 min after the last sprint. Blood flow will be determined by administration of Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be registrated continuously during the total investigation.
SUPPORT: This study is supported by grants from the Swedish National Center for Research in Sports
- Conditions
- Metabolism in Adipose Tissue
- Being tested
- Repeated 30-s sprint exercise
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Repeated 30-s sprint exerciseOther
Analyze of Interleukin-6 concentration before and after repeated 30-s sprint exercise
| Study group | Role | What they receive |
|---|---|---|
| Sprint exercise | Other | Three bouts of 30-s sprint exercise with 20 min rest in between. Three fat biopsies obtained ar rest before first sprint, 15 min after and 120 min after third sprint Blood samples from stomach vein and arteria during the whole experiment Receives: Other: Repeated 30-s sprint exercise |
What is being measured
The primary outcome is the main question the study is designed to answer.
Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise
Stomach fat biopsies and arterial and stomach vein blood are obtained
Measured: Before 30-s repeated sprint exercise
Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise
Stomach fat biopsies and arterial and stomach vein blood are obtained
Measured: After 30-s repeated sprint exercise
Secondary outcomes (4)
Changes in blood flow in adipose tissue
Xenon-133 is administered by a subcutaneous injection into the abdominal adipose tissue of approximately 1-2 MBq of Xenon-133 in the form of gas, at rest before work.
Measured: Before 30-s repeated sprint exercise
Changes in blood flow in adipose tissue
The rinsing rate of Xenon-133 from the tissue depot is continuously recorded during the study period by a non-invasive method using a portable scintillation detector system (Mediscint) up to 60 minutes after the last sprint work.
Measured: After 30-s repeated sprint exercise
Changes in different metabolites
Analysed for lactate, ammonia and IL-6
Measured: Before 30-s repeated sprint exercise
Changes in different metabolites
Analysed for lactate, ammonia and IL-6
Measured: After 30-s repeated sprint exercise
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted28 Sep 2020
- Study startSep 2015
- Primary completionSep 2016
- Study completionSep 2017
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Metabolism in Adipose TissueStudies of the same condition, open and under way.
- More trials run by Karolinska InstitutetThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Enrolment
- 18 participants (actual)
Sponsor and investigators
- Lead sponsor
- Karolinska Institutet
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Mona Esbjörnsson, Karolinska Institutet
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT04566354
- Sponsor's protocol ID
- 2015/1054-32
- Status last verified
- Sep 2020
- First submitted
- 22 Sep 2015
- Last updated
- 28 Sep 2020
- Results posted
- No
- Data monitoring committee
- No
Study at a glance
From the registry record, last updated by the sponsor on 28 Sep 2020. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Metabolism in Adipose Tissue Followed Following Sprint Exercise
- ClinicalTrials.gov ID
- NCT04566354
- Acronym / sponsor ID
- 2015/1054-32
- Conditions
- Metabolism in Adipose Tissue
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 18 participants (actual)
- Lead sponsor
- Karolinska InstitutetUniversity, hospital or other
- Study start
- Sep 2015
- Primary completion
- Sep 2016 (actual)
- Study completion
- Sep 2017 (actual)
- First posted
- 28 Sep 2020 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators will examine acute short-term exercise effects on adipose tissue IL-6 exchange.
Detailed description
PURPOSE: Low volume sprint exercise training has been shown to reduce body fat despite small total energy expenditure (for ref see Gillen and Gibala 2014). IL-6 has been shown to increase adipose tissue lipolysis in vitro. Therefore, in an initial study on effects of sprint exercise on adipose tissue metabolism the investigators examined acute short-term effects on adipose tissue IL-6 exchange.
METHODS: Four female and four male subjects perform repeated sprint exercise (3 x 30s with 20 min rest between; Wingate-test). Blood samples are repeatedly obtained, up to 120 min after the last sprint (9 min post ex), from catheters inserted percutaneously into brachial artery and a superficial subcutaneous vein on the anterior abdominal wall providing access to the venous drainage from the subcutaneous adipose tissue and analyzed for IL-6 by ELISA and lactate by spectrophotometry. Fat biopsies from the stomach will be obtained before, 15 min and 120 min after the last sprint. Blood flow will be determined by administration of Xenon-133 in the adipose tissue of the stomach. The release rate of Xenon-133 will be registrated continuously during the total investigation.
SUPPORT: This study is supported by grants from the Swedish National Center for Research in Sports
Study design
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Number of arms
- 1
| Arm | Type | Receives |
|---|---|---|
| Sprint exercise | Other | Other: Repeated 30-s sprint exerciseThree bouts of 30-s sprint exercise with 20 min rest in between. Three fat biopsies obtained ar rest before first sprint, 15 min after and 120 min after third sprint Blood samples from stomach vein and arteria during the whole experiment |
Who may qualify
- Ages
- 20 to 40 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (2 listed)
- Physical active
- Healthy
Key exclusion criteria (5 listed)
- Smokers
- Pregnant
- On medication
- No infection
- No asthma
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Repeated 30-s sprint exerciseAnalyze of Interleukin-6 concentration before and after repeated 30-s sprint exercise | Other | Sprint exercise |
Primary outcome
Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise
Time frame: Before 30-s repeated sprint exercise
Stomach fat biopsies and arterial and stomach vein blood are obtained
Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise
Time frame: After 30-s repeated sprint exercise
Stomach fat biopsies and arterial and stomach vein blood are obtained
4 secondary outcomes
Changes in blood flow in adipose tissue
Time frame: Before 30-s repeated sprint exercise
Changes in blood flow in adipose tissue
Time frame: After 30-s repeated sprint exercise
Changes in different metabolites
Time frame: Before 30-s repeated sprint exercise
Changes in different metabolites
Time frame: After 30-s repeated sprint exercise
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 28 Sep 2020.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Repeated 30-s sprint exercise in people with Metabolism in Adipose Tissue, run by Karolinska Institutet at 1 site in Sweden.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 2 inclusion and 5 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 28 Sep 2020. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Sweden. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Il-6 secretion in adipose tissue before and after 30-s repeated sprint exercise (time frame: Before 30-s repeated sprint exercise). The study lists 2 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 28 Sep 2020.