Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities3XNCT05365334
Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities
A study testing Low intensity exercise session, Moderate intensity exercise session and High intensity exercise session in people with Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities and Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile, run by University of Michigan at 1 site in United States.
Trial summary
The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3)… Read more about this study
- Who is it for?
- Ages 18 to 40 yearsHealthy volunteers can take part
- Conditions the study is for
- Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities, Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile, Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities
- What is being tested?
- Low intensity exercise session, Moderate intensity exercise session and High intensity exercise session
- Type of treatment
- Lifestyle or therapy programme
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 45 participants
- Where
- 1 siteUnited States
- Study dates
- 13 Dec 2021to 23 Jun 2023
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 40 years
- All sexes
- Healthy volunteers accepted
- Adult
Who may be able to join
- Age:18-40
- Body Mass Index: 20-30 kg/m2
- Must engage in regularly planned aerobic-type exercise at moderate-vigorous intensity, 3 times per week for at least 30-60 minutes/session
- Women must have regularly occurring menses and must be premenopausal
- Subject willingness to opt-in for the Michigan Genomics Initiative (MGI) Repository
Who cannot join
- Evidence/history of cardiovascular or metabolic disease.
- Taking anti-coagulate medications or medications known to affect metabolism or inflammation
- Weight instability ≥ ±3kg in the last 6 months
- Tobacco or e-cigarette users
- Women must not be pregnant or actively lactating
Trial locations and contacts
1 site in 1 country.
About this study
The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.
- Conditions
- Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities, Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile, Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities
- Being tested
- Low intensity exercise session, Moderate intensity exercise session, High intensity exercise session
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Low intensity exercise sessionBehavioural
subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes
Moderate intensity exercise sessionBehavioural
subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes
High intensity exercise sessionBehavioural
subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between
| Study group | Role | What they receive |
|---|---|---|
| low-intensity continuous exercise | Experimental | subjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes Receives: Behavioral: Low intensity exercise session |
| moderate-intensity continuous exercise | Experimental | subjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes Receives: Behavioral: Moderate intensity exercise session |
| high-intensity interval exercise | Experimental | subjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between Receives: Behavioral: High intensity exercise session |
What is being measured
The primary outcome is the main question the study is designed to answer.
post-translational modifications of skeletal muscle oxidative enzymes
Western blot
Measured: 30 minutes before exercise
post-translational modifications of skeletal muscle oxidative enzymes
Western blot
Measured: immediately after exercise
post-translational modifications of skeletal muscle oxidative enzymes
Western blot
Measured: 60 minutes after exercise
post-translational modifications of Adipose tissue metabolic proteins
Western blot
Measured: 15 minutes before exercise
post-translational modifications of Adipose tissue metabolic proteins
Western blot
Measured: 75 minutes after exercise
mRNA expression of skeletal muscle oxidative enzymes
qPCR
Measured: 30 minutes before exercise
mRNA expression of skeletal muscle oxidative enzymes
qPCR
Measured: immediately after exercise
mRNA expression of skeletal muscle oxidative enzymes
qPCR
Measured: 60 minutes after exercise
mRNA expression of adipose tissue metabolic enzymes
qPCR
Measured: 15 minutes before exercise
mRNA expression of adipose tissue metabolic enzymes
qPCR
Measured: 75 minutes after exercise
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted9 May 2022
- Study start13 Dec 2021
- Primary completion23 Jun 2023
- Study completion23 Jun 2023
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Publications (1)Papers the sponsor has linked to this study.
- Other trials for Cellular Responses Within Skeletal Muscle in Response to Exercise of Different IntensitiesStudies of the same condition, open and under way.
- More trials run by University of MichiganThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Enrolment
- 45 participants (actual)
Sponsor and investigators
- Lead sponsor
- University of Michigan
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Jeffrey F Horowitz, University of Michigan
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT05365334
- Sponsor's protocol ID
- HUM00204857
- Acronym
- 3X
- Status last verified
- Jul 2024
- First submitted
- 8 Apr 2022
- Last updated
- 26 Jul 2024
- Results posted
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Publications
Ahn C, Zhang T, Rode T, Yang G, Chugh OK, Ellis S, Ghayur S, Mehta S, Salzman R, Jiang H, Parker SCJ, Burant CF, Horowitz JF. Molecular responses in abdominal subcutaneous adipose tissue after a session of endurance exercise: effects of exercise intensity. J Physiol. 2025 Nov;603(22):6895-6919. doi: 10.1113/JP289339. Epub 2025 Oct 12.
PubMed 41076654
Study at a glance
From the registry record, last updated by the sponsor on 26 Jul 2024. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Skeletal Muscle and Adipose Tissue Responses After Acute Exercise: Comparing Effects of Three Different Intensities
- ClinicalTrials.gov ID
- NCT05365334
- Acronym / sponsor ID
- 3X / HUM00204857
- Conditions
- Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities, Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile, Changes Within Adipose Tissue in Response to Exercise at the Three Different Intensities
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 45 participants (actual)
- Lead sponsor
- University of MichiganUniversity, hospital or other
- Study start
- 13 Dec 2021 (actual)
- Primary completion
- 23 Jun 2023 (actual)
- Study completion
- 23 Jun 2023 (actual)
- First posted
- 9 May 2022 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The study will assess the exercise-induced changes in chromatin accessibility and gene expression in a cell type-specific manner in skeletal muscle from healthy adults in response to exercise at three different intensities - 1) low-intensity continuous exercise, 2) moderate-intensity continuous exercise, and 3) high-intensity interval exercise.
Study design
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Number of arms
- 3
| Arm | Type | Receives |
|---|---|---|
| low-intensity continuous exercise | Experimental | Behavioral: Low intensity exercise sessionsubjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes |
| moderate-intensity continuous exercise | Experimental | Behavioral: Moderate intensity exercise sessionsubjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes |
| high-intensity interval exercise | Experimental | Behavioral: High intensity exercise sessionsubjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between |
Who may qualify
- Ages
- 18 to 40 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (5 listed)
- Age:18-40
- Body Mass Index: 20-30 kg/m2
- Must engage in regularly planned aerobic-type exercise at moderate-vigorous intensity, 3 times per week for at least 30-60 minutes/session
- Women must have regularly occurring menses and must be premenopausal
- Subject willingness to opt-in for the Michigan Genomics Initiative (MGI) Repository
Key exclusion criteria (5 listed)
- Evidence/history of cardiovascular or metabolic disease.
- Taking anti-coagulate medications or medications known to affect metabolism or inflammation
- Weight instability ≥ ±3kg in the last 6 months
- Tobacco or e-cigarette users
- Women must not be pregnant or actively lactating
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Low intensity exercise sessionsubjects will exercise at 30% maximal oxygen consumption (VO2max) for 45-60 minutes | Behavioural | low-intensity continuous exercise |
| Moderate intensity exercise sessionsubjects will exercise at 65% maximal oxygen consumption (VO2max) for 30-45 minutes | Behavioural | moderate-intensity continuous exercise |
| High intensity exercise sessionsubjects will completed 10x1minute exercise intervals at maximal oxygen consumption (VO2max) with 1 minute active recovery between | Behavioural | high-intensity interval exercise |
Primary outcome
post-translational modifications of skeletal muscle oxidative enzymes
Time frame: 30 minutes before exercise
Western blot
post-translational modifications of skeletal muscle oxidative enzymes
Time frame: immediately after exercise
Western blot
post-translational modifications of skeletal muscle oxidative enzymes
Time frame: 60 minutes after exercise
Western blot
post-translational modifications of Adipose tissue metabolic proteins
Time frame: 15 minutes before exercise
Western blot
post-translational modifications of Adipose tissue metabolic proteins
Time frame: 75 minutes after exercise
Western blot
mRNA expression of skeletal muscle oxidative enzymes
Time frame: 30 minutes before exercise
qPCR
mRNA expression of skeletal muscle oxidative enzymes
Time frame: immediately after exercise
qPCR
mRNA expression of skeletal muscle oxidative enzymes
Time frame: 60 minutes after exercise
qPCR
mRNA expression of adipose tissue metabolic enzymes
Time frame: 15 minutes before exercise
qPCR
mRNA expression of adipose tissue metabolic enzymes
Time frame: 75 minutes after exercise
qPCR
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 26 Jul 2024.
- Derived from this study: PubMed 41076654Ahn C, Zhang T, Rode T, Yang G, Chugh OK, Ellis S, Ghayur S, Mehta S, Salzman R, Jiang H, Parker SCJ, Burant CF, Horowitz JF. Molecular responses in abdominal subcutaneous adipose tissue after a session of endurance exercise: effects of exercise intensity. J Physiol. 2025 Nov;603(22):6895-6919. doi: 10.1113/JP289339. Epub 2025 Oct 12.
Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Low intensity exercise session, Moderate intensity exercise session and High intensity exercise session in people with Cellular Responses Within Skeletal Muscle in Response to Exercise of Different Intensities and Relationship Between the Cellular Responses in Skeletal Muscle and the Changes in the Metabolomic Profile, run by University of Michigan at 1 site in United States.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 5 inclusion and 5 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 26 Jul 2024. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in United States. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
post-translational modifications of skeletal muscle oxidative enzymes (time frame: 30 minutes before exercise). The study lists 10 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
On the ClinicalTrials.gov record for NCT05365334, which also lists 1 linked publication.
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 26 Jul 2024; AzTrials last refreshed its copy on 6 Nov 2026.