HypertrophyNCT05544955
Protein Ingestion Timing on Body Composition and Biochemical Markers in Resistance-trained Males
A study testing isolate whey protein prior to and immediately after resistance training and isolate whey protein 3 hours prior to and 3 hours after resistance training in people with Hypertrophy and Physical Performance, run by Marymount University at 1 site in United States.
Trial summary
Protein ingestion timing is an important component for muscle accretion. The investigators hypothesized that protein ingestion timing could affect muscular adaptations and performance in resistance-trained men. Read more about this study
- Who is it for?
- Ages 18 to 35 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Hypertrophy, Physical Performance
- What is being tested?
- isolate whey protein prior to and immediately after resistance training and isolate whey protein 3 hours prior to and 3 hours after resistance training
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 40 participants
- Where
- 1 siteUnited States
- Study dates
- 17 Sep 2022to 31 Dec 2022
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 35 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- performing resistance training at least three times a week for 1 year prior to the start of the study
- not taking any steroids or any illegal agents known to increase muscle size for at least 1 year prior to the start of the study
- no known medical issues
- sleeping for at least seven to eight hours during the 24-hour day
Who cannot join
- non-willingness to continue protein ingestion or exercise protocols
- participation in exercise other than the prescribed resistance training program
- consumption of dietary supplement (other than the protein intervention) during the study period
- Missing more than one resistance training session or protein ingestion throughout the study period
Trial locations and contacts
1 site in 1 country.
About this study
Official title: Comparison of Protein Ingestion Timing on Body Composition, Physical Performance, and Biochemical Markers in Resistance-trained Males
Protein ingestion timing is an important component for muscle accretion. The investigators hypothesized that protein ingestion timing could affect muscular adaptations and performance in resistance-trained men.
- Conditions
- Hypertrophy, Physical Performance
- Being tested
- isolate whey protein prior to and immediately after resistance training, isolate whey protein 3 hours prior to and 3 hours after resistance training
- Medical subject headings
- Hypertrophy
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
isolate whey protein prior to and immediately after resistance trainingDietary supplement
This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.
isolate whey protein 3 hours prior to and 3 hours after resistance trainingDietary supplement
This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing.
| Study group | Role | What they receive |
|---|---|---|
| Protein ingestion immediately prior to and immediately after resistance training | Experimental | This arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing. Receives: Dietary Supplement: isolate whey protein prior to and immediately after resistance training |
| Protein ingestion 3 hours prior to and 3 hours after resistance training | Experimental | This arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing. Receives: Dietary Supplement: isolate whey protein 3 hours prior to and 3 hours after resistance training |
What is being measured
The primary outcome is the main question the study is designed to answer.
Body Composition
Total Lean mass, fat mass, and estimated VAT (g) will be assessed using whole-body DXA
Measured: 12 weeks
Secondary outcomes (7)
Muscular Strength
Using 1 repetition maximum
Measured: 12 weeks
Muscular Endurance
Using leg- and chest press exercises at 75% of the 1-RM
Measured: 12 weeks
Jump testing
Using the vertical and broad jump tests
Measured: 12 weeks
Pull-ups
Using the pull-up test
Measured: 12 weeks
Liver enzymes (ALT, AST, and GGT)
Using Fasting blood samples
Measured: 12 weeks
Creatinine and Urea Nitrogen
Urine samples will be collected using standard procedures
Measured: 12 weeks
Cholesterol Levels
Using Fasting blood samples
Measured: 12 weeks
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted19 Sep 2022
- Study start17 Sep 2022
- Primary completion31 Dec 2022
- Study completion31 Dec 2022
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for HypertrophyStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Triple blind
- Who is masked
- Participant, Investigator, Outcomes assessor
- Enrolment
- 40 participants (actual)
Sponsor and investigators
- Lead sponsor
- Marymount University
- Sponsor type
- University, hospital or other
- Collaborators
- Shahid Beheshti University of Medical Sciences
- Responsible party
- Principal investigator, Alexei Wong, Marymount University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT05544955
- Sponsor's protocol ID
- IR.SSRC.REC.1403.059
- Status last verified
- Jun 2025
- First submitted
- 12 Sep 2022
- Last updated
- 11 Jun 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Study at a glance
From the registry record, last updated by the sponsor on 11 Jun 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Comparison of Protein Ingestion Timing on Body Composition, Physical Performance, and Biochemical Markers in Resistance-trained Males
- ClinicalTrials.gov ID
- NCT05544955
- Acronym / sponsor ID
- IR.SSRC.REC.1403.059
- Conditions
- Hypertrophy, Physical Performance
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 40 participants (actual)
- Lead sponsor
- Marymount UniversityUniversity, hospital or other
- Collaborators
- Shahid Beheshti University of Medical Sciences
- Study start
- 17 Sep 2022 (actual)
- Primary completion
- 31 Dec 2022 (actual)
- Study completion
- 31 Dec 2022 (actual)
- First posted
- 19 Sep 2022 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Protein ingestion timing is an important component for muscle accretion. The investigators hypothesized that protein ingestion timing could affect muscular adaptations and performance in resistance-trained men.
Study design
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Triple blind (participant, investigator, outcomes assessor)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| Protein ingestion immediately prior to and immediately after resistance training | Experimental | Dietary Supplement: isolate whey protein prior to and immediately after resistance trainingThis arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing. |
| Protein ingestion 3 hours prior to and 3 hours after resistance training | Experimental | Dietary Supplement: isolate whey protein 3 hours prior to and 3 hours after resistance trainingThis arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing. |
Who may qualify
- Ages
- 18 to 35 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (4 listed)
- performing resistance training at least three times a week for 1 year prior to the start of the study
- not taking any steroids or any illegal agents known to increase muscle size for at least 1 year prior to the start of the study
- no known medical issues
- sleeping for at least seven to eight hours during the 24-hour day
Key exclusion criteria (4 listed)
- non-willingness to continue protein ingestion or exercise protocols
- participation in exercise other than the prescribed resistance training program
- consumption of dietary supplement (other than the protein intervention) during the study period
- Missing more than one resistance training session or protein ingestion throughout the study period
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| isolate whey protein prior to and immediately after resistance trainingThis arm involved protein ingestion immediately prior to and immediately after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing. | Dietary supplement | Protein ingestion immediately prior to and immediately after resistance training |
| isolate whey protein 3 hours prior to and 3 hours after resistance trainingThis arm involved protein ingestion 3 hours prior to and 3 hours after resistance training for 12 weeks. Participants will consume 40 g of isolate whey protein at their recommended timing. | Dietary supplement | Protein ingestion 3 hours prior to and 3 hours after resistance training |
Primary outcome
Body Composition
Time frame: 12 weeks
Total Lean mass, fat mass, and estimated VAT (g) will be assessed using whole-body DXA
7 secondary outcomes
Muscular Strength
Time frame: 12 weeks
Muscular Endurance
Time frame: 12 weeks
Jump testing
Time frame: 12 weeks
Pull-ups
Time frame: 12 weeks
Liver enzymes (ALT, AST, and GGT)
Time frame: 12 weeks
Creatinine and Urea Nitrogen
Time frame: 12 weeks
Cholesterol Levels
Time frame: 12 weeks
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 11 Jun 2025.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing isolate whey protein prior to and immediately after resistance training and isolate whey protein 3 hours prior to and 3 hours after resistance training in people with Hypertrophy and Physical Performance, run by Marymount University at 1 site in United States.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 4 inclusion and 4 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 11 Jun 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in United States. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Body Composition (time frame: 12 weeks). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 11 Jun 2025.