Sedentary BehaviourNCT06106464
Cardiometabolic Effects of Aerobic Exercise Plus Breaking Sitting
A study testing Aerobic exercise+6.5 h of prolonged sitting, Aerobic exercise+2 minutes of walking breaks every 30 minutes and Aerobic exercise+4 minutes of walking breaks every 1-h in people with Sedentary Behaviour and Sedentary Time, run by Yasin Ak at 1 site in Turkey (Türkiye).
Trial summary
The goal of this randomized crossover clinical trial is to investigate the effects of interrupting prolonged sitting with intermittent walks of equal volume but at different frequencies, in addition to moderate-intensity aerobic exercise, on postprandial serum lipid profile in young adults between the ages of 18-30. Read more about this study
- Who is it for?
- Ages 18 to 30 yearsHealthy volunteers can take part
- Conditions the study is for
- Sedentary Behaviour, Sedentary Time
- What is being tested?
- Aerobic exercise+6.5 h of prolonged sitting, Aerobic exercise+2 minutes of walking breaks every 30 minutes and Aerobic exercise+4 minutes of walking breaks every 1-h
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 14 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 12 Jun 2023to 1 Feb 2024
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 30 years
- All sexes
- Healthy volunteers accepted
- Adult
Who may be able to join
- Being between the ages of 18-30
- Being able to participate in physical activity
- More than 7 hours of daily sitting time measured subjectively
- Not exercising regularly in the last 3 months
Who cannot join
- Hypertension
- Coronary heart disease
- Heart failure
- Diabetes mellitus
- Metabolic syndrome
- Obesity
Show all 22Show fewer
- Hypothyroidism
- Non-alcoholic fatty liver disease
- Chronic inflammatory joint disease
- Chronic renal failure
- Cushing's syndrome
- HIV infection
- Being on antihypertensive medication
- Being on lipid-lowering medication
- Being on antidiabetic medication
- Being on systemic glucocorticoid medication
- LDL-cholesterol≥130 mg.dL-1
- HDL-cholesterol <40 mg.dL-1 for men; <50 mg.dL-1 for women
- Prediabetes
- Alcohol addiction
- Smoking
- Pregnancy
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Kastamonu University, Çatalzeytin Vocational School
Kastamonu, 37
About this study
Official title: Acute Cardiometabolic Effects of Moderate-Intensity Aerobic Exercise Plus Interrupting Sitting With Different Frequencies of Low-Intensity Physical Activity-A Randomized Crossover Trial
The goal of this randomized crossover clinical trial is to investigate the effects of interrupting prolonged sitting with intermittent walks of equal volume but at different frequencies, in addition to moderate-intensity aerobic exercise, on postprandial serum lipid profile in young adults between the ages of 18-30. The main questions it aims to answer are:
- Do interrupting prolonged sitting with intermittent walks of equal volume but at different frequencies sustain positive cardiometabolic effects after moderate-intensity aerobic exercise?
- If "yes", which frequency of breaks is more effective to provide cardiometabolic benefits?
Participants will complete four conditions on separate days: 1) 6.5 hours of prolonged sitting after 30 minutes of moderate intensity aerobic exercise 2) breaking prolonged sitting with 2 minutes of walking every 30 minutes after 30 minutes of moderate intensity exercise 3) breaking prolonged sitting with 4 minutes of walking every 1-h after 30 minutes of moderate intensity exercise 4) breaking prolonged sitting with 8 minutes of walking every 2-h after 30 minutes of moderate intensity aerobic exercise.
- Conditions
- Sedentary Behaviour, Sedentary Time
- Being tested
- Aerobic exercise+6.5 h of prolonged sitting, Aerobic exercise+2 minutes of walking breaks every 30 minutes, Aerobic exercise+4 minutes of walking breaks every 1-h, Aerobic exercise+8 minutes of walking breaks every 2-h
- Keywords
- sedentary break, prolonged sitting, blood lipids, blood pressure, aerobic exercise, walking
- Medical subject headings
- Sedentary Behavior
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Aerobic exercise+6.5 h of prolonged sittingOther
Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then sit in the chair for 6.5 hours. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.
Aerobic exercise+2 minutes of walking breaks every 30 minutesOther
Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 2-minute walks at 35-40% of their heart rate reserve every 30 minutes for 6.5 hours after moderate-intensity aerobic exercise. They will make a twelve interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.
Aerobic exercise+4 minutes of walking breaks every 1-hOther
Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 4-minute walks at 35-40% of their heart rate reserve every 1 hour for 6.5 hours after moderate-intensity aerobic exercise. They will make six interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.
Aerobic exercise+8 minutes of walking breaks every 2-hOther
Volunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 8-minute walks at 35-40% of their heart rate reserve every 2 hour for 6.5 hours after moderate-intensity aerobic exercise. They will make six interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured.
| Study group | Role | What they receive |
|---|---|---|
| Aerobic exercise+6.5 h of prolonged sitting | Experimental | Participants will sit 6.5 hours after 30 minutes of moderate intensity aerobic exercise. Receives: Other: Aerobic exercise+6.5 h of prolonged sitting |
| Aerobic exercise+2 minutes of walking breaks every 30 minutes | Experimental | Participants will break prolonged sitting with 2 minutes of walking every 30 minutes after 30 minutes of moderate intensity aerobic exercise. Receives: Other: Aerobic exercise+2 minutes of walking breaks every 30 minutes |
| Aerobic exercise+4 minutes of walking breaks every 1-h | Experimental | Participants will break prolonged sitting with 4 minutes of walking every 1-h after 30 minutes of moderate intensity aerobic exercise. Receives: Other: Aerobic exercise+4 minutes of walking breaks every 1-h |
| Aerobic exercise+8 minutes of walking breaks every 2-h | Experimental | Participants will break prolonged sitting with 8 minutes of walking every 2-h after 30 minutes of moderate intensity aerobic exercise. Receives: Other: Aerobic exercise+8 minutes of walking breaks every 2-h |
What is being measured
The primary outcome is the main question the study is designed to answer.
Capillary glucose area under the curve
Fasting and subsequent capillary blood glucose measurements will be carried out. Blood samples will be taken by pricking the tip of the distal phalanx of the right or left middle finger with a lancet. The first drop of blood will be wiped with cotton and the second drop of blood will be analyzed with a glucometer (GL 44, Beurer GmbH, Germany). Two consecutive measurements will be made and the average of both measurements will be recorded. For each 7.5-hour intervention, the total area under the curve (AUC) will be calculated for capillary glucose using the trapezoidal method. Incremental AUC will then be calculated by subtracting the area of baseline level from the total AUC.
Measured: During the four 7.5-hour intervention sessions samples will be collected at the 30th, 60th, 90th, 120th and 210th minutes after breakfast, and at the 30th, 60th, 90th, 120th and 210th minutes after lunch.
Serum Low-Density Lipoprotein cholesterol area under the curve
Fasting venous blood samples for serum LDL cholesterol will be collected by inserting a cannula into the appropriate vein. The intravenous cannula will be fixed tightly with patch tape. Then fasting blood samples will be drawn. During each 7.5-hour intervention, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch from the intravenous cannula with a syringe. The blood samples taken into the syringe will be transferred to a yellow capped gel tube and the blood samples will be sent to the biochemistry laboratory for analysis.For each 7.5-hour intervention, the total AUC will be calculated for serum LDL cholesterol using the trapezoidal method. Incremental AUC will then be calculated by subtracting the area of baseline level from the total AUC.
Measured: During the four 7.5-hour intervention sessions, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch
Serum High-Density Lipoprotein cholesterol area under the curve
Fasting venous blood samples for serum HDL cholesterol will be collected by inserting a cannula into the appropriate vein. The intravenous cannula will be fixed tightly with patch tape. Then fasting blood samples will be drawn. During each 7.5-hour intervention, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch from the intravenous cannula with a syringe. The blood samples taken into the syringe will be transferred to a yellow capped gel tube and the blood samples will be sent to the biochemistry laboratory for analysis.For each 7.5-hour intervention, the total AUC will be calculated for serum HDL cholesterol using the trapezoidal method. Incremental AUC will then be calculated by subtracting the area of baseline level from the total AUC.
Measured: During the four 7.5-hour intervention sessions, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch
Serum total cholesterol area under the curve
Fasting venous blood samples for serum total cholesterol will be collected by inserting a cannula into the appropriate vein. The intravenous cannula will be fixed tightly with patch tape. Then fasting blood samples will be drawn. During each 7.5-hour intervention, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch from the intravenous cannula with a syringe. The blood samples taken into the syringe will be transferred to a yellow capped gel tube and the blood samples will be sent to the biochemistry laboratory for analysis.For each 7.5-hour intervention, the total AUC will be calculated for serum total cholesterol using the trapezoidal method. Incremental AUC will then be calculated by subtracting the area of baseline level from the total AUC.
Measured: During the four 7.5-hour intervention sessions, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch
Serum triglycerides area under the curve
Fasting venous blood samples for serum triglycerides will be collected by inserting a cannula into the appropriate vein. The intravenous cannula will be fixed tightly with patch tape. Then fasting blood samples will be drawn. During each 7.5-hour intervention, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch from the intravenous cannula with a syringe. The blood samples taken into the syringe will be transferred to a yellow capped gel tube and the blood samples will be sent to the biochemistry laboratory for analysis.For each 7.5-hour intervention, the total AUC will be calculated for serum triglycerides using the trapezoidal method. Incremental AUC will then be calculated by subtracting the area of baseline level from the total AUC.
Measured: During the four 7.5-hour intervention sessions, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch
Secondary outcomes (2)
Systolic blood pressure
Systolic blood pressures will be measured in the arm in the morning before each intervention and at regular intervals during each intervention. Measurements will be taken in an upright position just before blood samples are taken and after a rest period of at least 10 minutes from the last exercise bout. Blood pressure measurements will be taken with a digital blood pressure monitor (BM 40, Beurer GmbH, Germany) in the arm. The first measurement will be taken at approximately 08:30 a.m and the next 7 measurements will be carried out approximately at the 20th minute of each hour (10:20, 11:20, 12:20, 13:20, 14:20, 15:20, 16:20). Three measurements will be made at one-minute intervals and the average value of the three measurements will be recorded.
Measured: During the four 7.5-hour intervention sessions, measurements will be carried out approximately at the 20th minute of each hour (10:20, 11:20, 12:20, 13:20, 14:20, 15:20, 16:20)
Diastolic blood pressure
Diastolic blood pressures will be measured in the arm in the morning before each intervention and at regular intervals during each intervention. Measurements will be taken in an upright position just before blood samples are taken and after a rest period of at least 10 minutes from the last exercise bout. Blood pressure measurements will be taken with a digital blood pressure monitor (BM 40, Beurer GmbH, Germany) in the arm. The first measurement will be taken at approximately 08:30 a.m and the next 7 measurements will be carried out approximately at the 20th minute of each hour (10:20, 11:20, 12:20, 13:20, 14:20, 15:20, 16:20). Three measurements will be made at one-minute intervals and the average value of the three measurements will be recorded.
Measured: During the four 7.5-hour intervention sessions, measurements will be carried out approximately at the 20th minute of each hour (10:20, 11:20, 12:20, 13:20, 14:20, 15:20, 16:20)
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted30 Oct 2023
- Study start12 Jun 2023
- Primary completion1 Jan 2024
- Study completion1 Feb 2024
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Publications (1)Papers the sponsor has linked to this study.
- Other trials for Sedentary BehaviourStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Single blind
- Who is masked
- Outcomes assessor
- Enrolment
- 14 participants (actual)
Sponsor and investigators
- Lead sponsor
- Yasin Ak
- Sponsor type
- University, hospital or other
- Responsible party
- Sponsor-investigator, Yasin Ak, Kastamonu University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT06106464
- Sponsor's protocol ID
- LIPA Break
- Status last verified
- Feb 2024
- First submitted
- 24 Oct 2023
- Last updated
- 13 Feb 2024
- Results posted
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Publications
Ak Y, Guzel Y, Akyel S, Tatar OD, Karabulut E, Karaca A. Hourly 4-minute walking breaks from sitting following aerobic exercise reduce postprandial non-HDL cholesterol in healthy young adults: A randomized crossover trial. J Clin Lipidol. 2025 May-Jun;19(3):638-648. doi: 10.1016/j.jacl.2025.03.007. Epub 2025 Mar 21.
PubMed 40246606
Study at a glance
From the registry record, last updated by the sponsor on 13 Feb 2024. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Acute Cardiometabolic Effects of Moderate-Intensity Aerobic Exercise Plus Interrupting Sitting With Different Frequencies of Low-Intensity Physical Activity-A Randomized Crossover Trial
- ClinicalTrials.gov ID
- NCT06106464
- Acronym / sponsor ID
- LIPA Break
- Conditions
- Sedentary Behaviour, Sedentary Time
- Keywords
- sedentary break, prolonged sitting, blood lipids, blood pressure, aerobic exercise, walking
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 14 participants (actual)
- Lead sponsor
- Yasin AkUniversity, hospital or other
- Study start
- 12 Jun 2023 (actual)
- Primary completion
- 1 Jan 2024 (actual)
- Study completion
- 1 Feb 2024 (actual)
- First posted
- 30 Oct 2023 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The goal of this randomized crossover clinical trial is to investigate the effects of interrupting prolonged sitting with intermittent walks of equal volume but at different frequencies, in addition to moderate-intensity aerobic exercise, on postprandial serum lipid profile in young adults between the ages of 18-30. The main questions it aims to answer are:
- Do interrupting prolonged sitting with intermittent walks of equal volume but at different frequencies sustain positive cardiometabolic effects after moderate-intensity aerobic exercise?
- If "yes", which frequency of breaks is more effective to provide cardiometabolic benefits?
Participants will complete four conditions on separate days: 1) 6.5 hours of prolonged sitting after 30 minutes of moderate intensity aerobic exercise 2) breaking prolonged sitting with 2 minutes of walking every 30 minutes after 30 minutes of moderate intensity exercise 3) breaking prolonged sitting with 4 minutes of walking every 1-h after 30 minutes of moderate intensity exercise 4) breaking prolonged sitting with 8 minutes of walking every 2-h after 30 minutes of moderate intensity aerobic exercise.
Study design
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Single blind (outcomes assessor)
- Number of arms
- 4
| Arm | Type | Receives |
|---|---|---|
| Aerobic exercise+6.5 h of prolonged sitting | Experimental | Other: Aerobic exercise+6.5 h of prolonged sittingParticipants will sit 6.5 hours after 30 minutes of moderate intensity aerobic exercise. |
| Aerobic exercise+2 minutes of walking breaks every 30 minutes | Experimental | Other: Aerobic exercise+2 minutes of walking breaks every 30 minutesParticipants will break prolonged sitting with 2 minutes of walking every 30 minutes after 30 minutes of moderate intensity aerobic exercise. |
| Aerobic exercise+4 minutes of walking breaks every 1-h | Experimental | Other: Aerobic exercise+4 minutes of walking breaks every 1-hParticipants will break prolonged sitting with 4 minutes of walking every 1-h after 30 minutes of moderate intensity aerobic exercise. |
| Aerobic exercise+8 minutes of walking breaks every 2-h | Experimental | Other: Aerobic exercise+8 minutes of walking breaks every 2-hParticipants will break prolonged sitting with 8 minutes of walking every 2-h after 30 minutes of moderate intensity aerobic exercise. |
Who may qualify
- Ages
- 18 to 30 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (4 listed)
- Being between the ages of 18-30
- Being able to participate in physical activity
- More than 7 hours of daily sitting time measured subjectively
- Not exercising regularly in the last 3 months
Key exclusion criteria (22 listed)
- Hypertension
- Coronary heart disease
- Heart failure
- Diabetes mellitus
- Metabolic syndrome
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Aerobic exercise+6.5 h of prolonged sittingVolunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then sit in the chair for 6.5 hours. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured. | Other | Aerobic exercise+6.5 h of prolonged sitting |
| Aerobic exercise+2 minutes of walking breaks every 30 minutesVolunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 2-minute walks at 35-40% of their heart rate reserve every 30 minutes for 6.5 hours after moderate-intensity aerobic exercise. They will make a twelve interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured. | Other | Aerobic exercise+2 minutes of walking breaks every 30 minutes |
| Aerobic exercise+4 minutes of walking breaks every 1-hVolunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 4-minute walks at 35-40% of their heart rate reserve every 1 hour for 6.5 hours after moderate-intensity aerobic exercise. They will make six interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured. | Other | Aerobic exercise+4 minutes of walking breaks every 1-h |
| Aerobic exercise+8 minutes of walking breaks every 2-hVolunteers, whose fasting blood samples are drawn and who eat a standard breakfast, will perform a 30-minute walking exercise on a treadmill at 40-45% of their heart rate reserve. They will then interrupt sitting with 8-minute walks at 35-40% of their heart rate reserve every 2 hour for 6.5 hours after moderate-intensity aerobic exercise. They will make six interruptions throughout 6.5 hours period and will have a total of 24 minutes of interruption throughout the intervention. While seated, volunteers will be asked not to leave their seats except to use the toilet. Volunteers will be able to read and use computers and phones while sitting. Venous and capillary blood samples will be drawn at regular intervals and their blood pressures will be measured. | Other | Aerobic exercise+8 minutes of walking breaks every 2-h |
Primary outcome
Capillary glucose area under the curve
Time frame: During the four 7.5-hour intervention sessions samples will be collected at the 30th, 60th, 90th, 120th and 210th minutes after breakfast, and at the 30th, 60th, 90th, 120th and 210th minutes after lunch.
Fasting and subsequent capillary blood glucose measurements will be carried out. Blood samples will be taken by pricking the tip of the distal phalanx of the right or left middle finger with a lancet. The first drop of blood will be wiped with cotton and the second drop of blood will be analyzed with a glucometer (GL 44, Beurer GmbH, Germany). Two consecutive measurements will be made and the average of both measurements will be recorded. For each 7.5-hour intervention, the total area under the curve (AUC) will be calculated for capillary glucose using the trapezoidal method. Incremental AUC will then be calculated by subtracting the area of baseline level from the total AUC.
Serum Low-Density Lipoprotein cholesterol area under the curve
Time frame: During the four 7.5-hour intervention sessions, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch
Fasting venous blood samples for serum LDL cholesterol will be collected by inserting a cannula into the appropriate vein. The intravenous cannula will be fixed tightly with patch tape. Then fasting blood samples will be drawn. During each 7.5-hour intervention, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch from the intravenous cannula with a syringe. The blood samples taken into the syringe will be transferred to a yellow capped gel tube and the blood samples will be sent to the biochemistry laboratory for analysis.For each 7.5-hour intervention, the total AUC will be calculated for serum LDL cholesterol using the trapezoidal method. Incremental AUC will then be calculated by subtracting the area of baseline level from the total AUC.
Serum High-Density Lipoprotein cholesterol area under the curve
Time frame: During the four 7.5-hour intervention sessions, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch
Fasting venous blood samples for serum HDL cholesterol will be collected by inserting a cannula into the appropriate vein. The intravenous cannula will be fixed tightly with patch tape. Then fasting blood samples will be drawn. During each 7.5-hour intervention, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch from the intravenous cannula with a syringe. The blood samples taken into the syringe will be transferred to a yellow capped gel tube and the blood samples will be sent to the biochemistry laboratory for analysis.For each 7.5-hour intervention, the total AUC will be calculated for serum HDL cholesterol using the trapezoidal method. Incremental AUC will then be calculated by subtracting the area of baseline level from the total AUC.
Serum total cholesterol area under the curve
Time frame: During the four 7.5-hour intervention sessions, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch
Fasting venous blood samples for serum total cholesterol will be collected by inserting a cannula into the appropriate vein. The intravenous cannula will be fixed tightly with patch tape. Then fasting blood samples will be drawn. During each 7.5-hour intervention, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch from the intravenous cannula with a syringe. The blood samples taken into the syringe will be transferred to a yellow capped gel tube and the blood samples will be sent to the biochemistry laboratory for analysis.For each 7.5-hour intervention, the total AUC will be calculated for serum total cholesterol using the trapezoidal method. Incremental AUC will then be calculated by subtracting the area of baseline level from the total AUC.
Serum triglycerides area under the curve
Time frame: During the four 7.5-hour intervention sessions, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch
Fasting venous blood samples for serum triglycerides will be collected by inserting a cannula into the appropriate vein. The intravenous cannula will be fixed tightly with patch tape. Then fasting blood samples will be drawn. During each 7.5-hour intervention, 5 mL blood samples will be drawn at the 60th, 120th and 210th minutes after breakfast and at the 60th, 120th and 210th minutes after lunch from the intravenous cannula with a syringe. The blood samples taken into the syringe will be transferred to a yellow capped gel tube and the blood samples will be sent to the biochemistry laboratory for analysis.For each 7.5-hour intervention, the total AUC will be calculated for serum triglycerides using the trapezoidal method. Incremental AUC will then be calculated by subtracting the area of baseline level from the total AUC.
2 secondary outcomes
Systolic blood pressure
Time frame: During the four 7.5-hour intervention sessions, measurements will be carried out approximately at the 20th minute of each hour (10:20, 11:20, 12:20, 13:20, 14:20, 15:20, 16:20)
Diastolic blood pressure
Time frame: During the four 7.5-hour intervention sessions, measurements will be carried out approximately at the 20th minute of each hour (10:20, 11:20, 12:20, 13:20, 14:20, 15:20, 16:20)
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 13 Feb 2024.
- Derived from this study: PubMed 40246606Ak Y, Guzel Y, Akyel S, Tatar OD, Karabulut E, Karaca A. Hourly 4-minute walking breaks from sitting following aerobic exercise reduce postprandial non-HDL cholesterol in healthy young adults: A randomized crossover trial. J Clin Lipidol. 2025 May-Jun;19(3):638-648. doi: 10.1016/j.jacl.2025.03.007. Epub 2025 Mar 21.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Aerobic exercise+6.5 h of prolonged sitting, Aerobic exercise+2 minutes of walking breaks every 30 minutes and Aerobic exercise+4 minutes of walking breaks every 1-h in people with Sedentary Behaviour and Sedentary Time, run by Yasin Ak at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 4 inclusion and 22 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 13 Feb 2024. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Capillary glucose area under the curve (time frame: During the four 7.5-hour intervention sessions samples will be collected at the 30th, 60th, 90th, 120th and 210th minutes after breakfast, and at the 30th, 60th, 90th, 120th and 210th minutes after lunch.). The study lists 5 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
On the ClinicalTrials.gov record for NCT06106464, which also lists 1 linked publication.
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 13 Feb 2024; AzTrials last refreshed its copy on 6 Nov 2026.