AgeingYOMANCT06332651
Metabolic Availability in Older and Younger Men
A study testing Black beans, Milk and Sorghum in people with Ageing, run by Wageningen University at 1 site in Netherlands.
Trial summary
The main goal of this study is to determine the metabolic availability of methionine in black beans, lysine in sorghum and lysine in milk using the indicator amino acid oxidation method both in older and in younger men. Read more about this study
- Who is it for?
- Ages 20 to 80 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Ageing
- What is being tested?
- Black beans, Milk and Sorghum
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 28 participants
- Where
- 1 siteNetherlands
- Study dates
- 19 Feb 2024to 15 Jul 2025
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 20 to 80 years
- Men only
- Healthy volunteers accepted
- Adult, Older adult
Who may be able to join
- Gender: Male Age: 20-35 years or 65-80 years Body Mass Index (BMI): 18.5 - 30.0 kg/m2 Healthy as assessed with a questionnaire Regular and normal eating habits as assessed with a Food Frequency Questionnaire (FFQ) Willing to comply with study procedures and use of data Having given written informed consent
Who cannot join
- Chronic disease, for example:
- Diabetes mellitus / being treated for high blood glucose
- Severe cardiovascular disease (exception: hypertension)
- Hepatic disease (e.g. hepatitis)
- Renal disease
- Cancer
Show all 32Show fewer
- Bowel disease (e.g. inflammatory bowel disease, ulcers, bleeding)
- Pancreatitis
- History of medical or surgical events that may affect GI function, and the study outcomes or disease risk for participants, for example:
- Bariatric surgery
- Gastrointestinal tract surgery
- Digestive tract disorder
- Chewing problems
- History of deep venous thrombosis without anti-coagulation medication
- Medicine use that interferes with, protein metabolism, GI function and the study outcomes, for example:
- Glucose lowering drugs
- Proton pump inhibitors
- Laxatives
- Habits that interfere with the study outcomes:
- Protein supplement use (current use or less than 2 weeks past use)
- Smoking
- Drug use
- Alcohol consumption >21 units/week and/or >4/day
- Following a weight-loss diet, medically prescribed diet or other diet with a low calorie intake or an unbalanced nutrient intake like a vegan or very low carbohydrate diet
- Moderate to high intense physical activity for more than 5 hours a week
- Other:
- Self-reported allergy or intolerance to the tested products
- Weight loss of more than 3 kg in the 3 months prior to study screening
- Current participation in other research and <2 months prior participation in other research
- Not having a general practitioner
- Not willing to accept information-transfer concerning participation in the study , or information regarding his health to his general practitioner
- Working or doing an MSc- thesis at the department of Human Nutrition and Health at Wageningen University \& Research
Trial locations and contacts
1 site in 1 country.
Netherlands1 site
Wageningen University & Research
Wageningen, Gelderland, 6708PB
About this study
Official title: Metabolic Availability of Three Protein Sources in Older and Younger Men as Measured With the Indicator Amino Acid Oxidation Method
The main goal of this study is to determine the metabolic availability of methionine in black beans, lysine in sorghum and lysine in milk using the indicator amino acid oxidation method both in older and in younger men.
- Conditions
- Ageing
- Being tested
- Black beans, Milk, Sorghum
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Black beansOther
Also called Nutrition
Black beans cooked and mashed and amino acid mixture as liquid drink to make methionine the limiting amino acid in the food consumed
MilkOther
Also called Nutrition
Skimmed milk as liquid drink and amino acid mixture as liquid drink to make lysine the limiting amino acid in the food consumed
SorghumOther
Also called Nutrition
Sorghum cooked and mashed and amino acid mixture as liquid drink to make lysine the limiting amino acid in the food consumed
| Study group | Role | What they receive |
|---|---|---|
| Methionine in black beans in older men | Experimental | This group of 7 participants will have 3 test days where they consume black beans with 3 different levels of methionine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of methionine. Receives: Other: Black beans |
| Methionine in black beans in younger men | Experimental | This group of 7 participants will have 3 test days where they consume black beans with 3 different levels of methionine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of methionine. Receives: Other: Black beans |
| Lysine in milk or sorghum in older men | Experimental | This group of 7 participants will have 3 test days where they consume milk with 3 different levels of lysine, they will have 3 test days where they consume sorghum with 3 different levels of lysine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of lysine. Receives: Other: Milk; Other: Sorghum |
| Lysine milk or sorghum in younger men | Experimental | This group of 7 participants will have 3 test days where they consume milk with 3 different levels of lysine, they will have 3 test days where they consume sorghum with 3 different levels of lysine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of lysine. Receives: Other: Milk; Other: Sorghum |
What is being measured
The primary outcome is the main question the study is designed to answer.
Metabolic availability of methionine in black beans and lysine in milk and sorghum measured with the indicator amino acid oxidation method.
Indicator amino acid oxidation method is used to measure metabolic availability of the limiting amino acid by measuring the oxidation of the 13C-labelled indicator amino acid in expired breath as 13CO2 when giving different levels of the limiting amino acid. Methionine metabolic availability will be assessed in black beans and lysine metabolic availability in milk and sorghum.
Measured: 1 measurement to be calculated from repeated measurements on several test days separated by 1 week
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted27 Mar 2024
- Study start19 Feb 2024
- Primary completion30 Jun 2025
- Study completion15 Jul 2025
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for AgeingStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Prevention
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Model description
- It is a parallel intervention study. The intervention does not involve a drug or biologic product, however does involve an adaptation diet and a diet with measured amino acids dissolved in water, during the test day. We investigate the amino acid metabolic availability using the IAAO method.
- Masking
- None (open label)
- Enrolment
- 28 participants (actual)
Sponsor and investigators
- Lead sponsor
- Wageningen University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Marco Mensink, Wageningen University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT06332651
- Sponsor's protocol ID
- NL84684.091.23
- Acronym
- YOMA
- Status last verified
- Jul 2025
- First submitted
- 19 Dec 2023
- Last updated
- 7 May 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Study at a glance
From the registry record, last updated by the sponsor on 7 May 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Metabolic Availability of Three Protein Sources in Older and Younger Men as Measured With the Indicator Amino Acid Oxidation Method
- ClinicalTrials.gov ID
- NCT06332651
- Acronym / sponsor ID
- YOMA / NL84684.091.23
- Conditions
- Ageing
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 28 participants (actual)
- Lead sponsor
- Wageningen UniversityUniversity, hospital or other
- Study start
- 19 Feb 2024 (actual)
- Primary completion
- 30 Jun 2025 (actual)
- Study completion
- 15 Jul 2025 (actual)
- First posted
- 27 Mar 2024 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The main goal of this study is to determine the metabolic availability of methionine in black beans, lysine in sorghum and lysine in milk using the indicator amino acid oxidation method both in older and in younger men.
Study design
- Primary purpose
- Prevention
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Number of arms
- 4
- Model description
- It is a parallel intervention study. The intervention does not involve a drug or biologic product, however does involve an adaptation diet and a diet with measured amino acids dissolved in water, during the test day. We investigate the amino acid metabolic availability using the IAAO method.
| Arm | Type | Receives |
|---|---|---|
| Methionine in black beans in older men | Experimental | Other: Black beansThis group of 7 participants will have 3 test days where they consume black beans with 3 different levels of methionine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of methionine. |
| Methionine in black beans in younger men | Experimental | Other: Black beansThis group of 7 participants will have 3 test days where they consume black beans with 3 different levels of methionine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of methionine. |
| Lysine in milk or sorghum in older men | Experimental | Other: Milk, Other: SorghumThis group of 7 participants will have 3 test days where they consume milk with 3 different levels of lysine, they will have 3 test days where they consume sorghum with 3 different levels of lysine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of lysine. |
| Lysine milk or sorghum in younger men | Experimental | Other: Milk, Other: SorghumThis group of 7 participants will have 3 test days where they consume milk with 3 different levels of lysine, they will have 3 test days where they consume sorghum with 3 different levels of lysine and they will have 4 test days where they consume the reference amino acid mixture with 4 different levels of lysine. |
Who may qualify
- Ages
- 20 to 80 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult, Older adult
Key inclusion criteria (1 listed)
- Gender: Male Age: 20-35 years or 65-80 years Body Mass Index (BMI): 18.5 - 30.0 kg/m2 Healthy as assessed with a questionnaire Regular and normal eating habits as assessed with a Food Frequency Questionnaire (FFQ) Willing to comply with study procedures and use of data Having given written informed consent
Key exclusion criteria (32 listed)
- Diabetes mellitus / being treated for high blood glucose
- Severe cardiovascular disease (exception: hypertension)
- Hepatic disease (e.g. hepatitis)
- Renal disease
- Cancer
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Black beansAlso: NutritionBlack beans cooked and mashed and amino acid mixture as liquid drink to make methionine the limiting amino acid in the food consumed | Other | Methionine in black beans in older men, Methionine in black beans in younger men |
| MilkAlso: NutritionSkimmed milk as liquid drink and amino acid mixture as liquid drink to make lysine the limiting amino acid in the food consumed | Other | Lysine in milk or sorghum in older men, Lysine milk or sorghum in younger men |
| SorghumAlso: NutritionSorghum cooked and mashed and amino acid mixture as liquid drink to make lysine the limiting amino acid in the food consumed | Other | Lysine in milk or sorghum in older men, Lysine milk or sorghum in younger men |
Primary outcome
Metabolic availability of methionine in black beans and lysine in milk and sorghum measured with the indicator amino acid oxidation method.
Time frame: 1 measurement to be calculated from repeated measurements on several test days separated by 1 week
Indicator amino acid oxidation method is used to measure metabolic availability of the limiting amino acid by measuring the oxidation of the 13C-labelled indicator amino acid in expired breath as 13CO2 when giving different levels of the limiting amino acid. Methionine metabolic availability will be assessed in black beans and lysine metabolic availability in milk and sorghum.
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 7 May 2026.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Black beans, Milk and Sorghum in people with Ageing, run by Wageningen University at 1 site in Netherlands.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 1 inclusion and 32 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 7 May 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Netherlands. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Metabolic availability of methionine in black beans and lysine in milk and sorghum measured with the indicator amino acid oxidation method. (time frame: 1 measurement to be calculated from repeated measurements on several test days separated by 1 week). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 7 May 2026; AzTrials last refreshed its copy on 6 Nov 2026.