Exercise PerformanceNCT06349018
Effects of Isotonic Beetroot Supplementation on Sprint Interval Exercise Performance
A study testing Dietary Supplement: High nitrate isotonic drink and Dietary Supplement: No nitrate isotonic drink in people with Exercise Performance, run by Nanyang Technological University at 1 site in Singapore.
Trial summary
This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9… Read more about this study
- Who is it for?
- Ages 21 to 40 yearsHealthy volunteers can take part
- Conditions the study is for
- Exercise Performance
- What is being tested?
- Dietary Supplement: High nitrate isotonic drink and Dietary Supplement: No nitrate isotonic drink
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 24 participants
- Where
- 1 siteSingapore
- Study dates
- 20 Feb 2024to 31 May 2024
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 21 to 40 years
- All sexes
- Healthy volunteers accepted
- Adult
Who may be able to join
- Age: 21 - 40 years
- Healthy and injury-free (e.g., no cardiovascular disease, diabetes mellitus, orthopaedic impairment that interferes with moderate-to-vigorous exercise)
- Blood pressure < 130/90 mmHg
- Be physically active: Engaging in a minimum of 150 minutes of moderate-intensity exercise or 75 minutes of vigorous-intensity physical activity each week.
- Recreational cyclist (Minimum 1 hour cycling per week).
- Body mass index: 18.5-25 kg/m2
- Non-smoker
- Asian
Who cannot join
- Any allergies to beetroot or isotonic drink.
- Asthma
- Any diagnosed form of cardiometabolic disease (heart disease, stroke, peripheral vascular disease, diabetes, metabolic syndrome, high blood pressure).
- Any symptoms contraindicating exercise testing (e.g. chest pains).
- Any balance or dizziness problems.
- Any chronic medical conditions (whether medicated or not).
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- Any bone joint problems.
- Any Physician diagnosed contraindications to exercise.
- Any other health problems that may be a cause for concern or contraindication to exercise.
- Any recent Covid vaccination (within last 2 weeks).
Trial locations and contacts
1 site in 1 country.
Singapore1 site
Human Bioenergetics Laboratory, National Institute of Education, Nanyang Technological University
Singapore, 637616
About this study
Official title: The Effects of Isotonic Beetroot Supplementation on Sprint Interval Exercise Performance.
This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9 mmol) for 5 days or (ii) an isotonic sports drink (no nitrate) for 5 days. On Day 5 of each trial, participants will come to the laboratory and 2 hours after consuming their allocated drink will complete a 6 bouts of sprint interval exercise. Power output during cycling will be measured as the primary outcome measure. Salivary and plasma measures of nitrate and nitrite will be taken over the course of the morning on Day 5. After a 5-day washout period participants will crossover and begin their second trial.
Read the detailed description
This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9 mmol) for 5 days or (ii) an isotonic sports drink (no nitrate) for 5 days. Drinks will be equivalent in isotonicity and colored the same so that participants and investigators will be blinded to each trial. Randomization and drink allocation will be completed by a third person not associated with the study team. On Day 5 of each trial, participants will come to the laboratory and 2 hours after consuming their allocated drink will complete a 6 bouts of sprint interval exercise (10 second all-out maximal sprints interspersed with 50 seconds rest). Power output (peak and mean) during cycling sprints will be measured as the primary outcome measure. Salivary measures of nitrate/nitrite will be taken at three time points over the course of the morning on Day 5 - (i) pre-drink consumption; (ii) 2 hours post-drink consumption; and (iii) post-exercise. Plasma measures of nitrate and nitrite will be taken at two time points over the course of the morning on Day 5 - (i) pre-drink consumption; and (ii) 2 hours post-drink consumption. Near infra-red spectroscopy (NIRS) will be used to measure muscle (vastus lateralis) oxygenation during cycling bouts. A Stroop test to measure inhibition (executive function) will be taken pre- and post-exercise. Sensory perception of the drink will be measured immediately post-consumption on Day 5. After a 5-day washout period participants will crossover and begin their second trial.
- Conditions
- Exercise Performance
- Being tested
- Dietary Supplement: High nitrate isotonic drink, Dietary Supplement: No nitrate isotonic drink
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Dietary Supplement: High nitrate isotonic drinkOther
High nitrate (12.9 mmol) drink with carbohydrate and minerals
Dietary Supplement: No nitrate isotonic drinkOther
Carbohydrate and mineral isotonic drink
| Study group | Role | What they receive |
|---|---|---|
| High nitrate | Experimental | High nitrate isotonic drink Receives: Other: Dietary Supplement: High nitrate isotonic drink |
| No nitrate | Active comparator | No nitrate isotonic drink Receives: Other: Dietary Supplement: No nitrate isotonic drink |
What is being measured
The primary outcome is the main question the study is designed to answer.
Power output during maximal sprint cycling
Peak and mean power output
Measured: 2 hours after drink ingestion
Secondary outcomes (5)
Salivary nitrate
Salivary nitrate/nitrite concentration
Measured: Pre-drink, 2 hours post-drink and immediately post-exercise (2.5 hours post-drink)
Plasma nitrate and nitrite
Plasma nitrate and nitrite
Measured: Pre-drink and 2 hours post-drink
Muscle oxygenation
Near infra-red spectroscopy
Measured: During exercise (2-2.5 hours post drink)
Sensory evaluation (Acceptance and Preference Test Evaluation by Meilgaard et al 2007)
Drink taste and perception (7-point scale - 1 extremely dislike to 7 extremely like)
Measured: Immediately post-drink (1 minute post-drink)
Stroop test
Reaction time and correct answers
Measured: Immediately pre- (2 hours post-drink) and immediately post-exercise (2.5 hours post drink)
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted5 Apr 2024
- Study start20 Feb 2024
- Primary completion31 May 2024
- Study completion31 May 2024
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Exercise PerformanceStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Double blind
- Who is masked
- Participant, Investigator
- Enrolment
- 24 participants (actual)
Sponsor and investigators
- Lead sponsor
- Nanyang Technological University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Stephen Burns, Nanyang Technological University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT06349018
- Sponsor's protocol ID
- IRB-2023-972
- Status last verified
- Apr 2025
- First submitted
- 20 Feb 2024
- Last updated
- 13 Apr 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- Yes: All published data will be placed into the Data Repository of Nanyang Technological University National Institute of Education for public access upon publication.
Study at a glance
From the registry record, last updated by the sponsor on 13 Apr 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Effects of Isotonic Beetroot Supplementation on Sprint Interval Exercise Performance.
- ClinicalTrials.gov ID
- NCT06349018
- Acronym / sponsor ID
- IRB-2023-972
- Conditions
- Exercise Performance
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 24 participants (actual)
- Lead sponsor
- Nanyang Technological UniversityUniversity, hospital or other
- Study start
- 20 Feb 2024 (actual)
- Primary completion
- 31 May 2024 (actual)
- Study completion
- 31 May 2024 (actual)
- First posted
- 5 Apr 2024 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9 mmol) for 5 days or (ii) an isotonic sports drink (no nitrate) for 5 days. On Day 5 of each trial, participants will come to the laboratory and 2 hours after consuming their allocated drink will complete a 6 bouts of sprint interval exercise. Power output during cycling will be measured as the primary outcome measure. Salivary and plasma measures of nitrate and nitrite will be taken over the course of the morning on Day 5. After a 5-day washout period participants will crossover and begin their second trial.
Detailed description
This study aims to examine the effects of dietary nitrate supplementation via beetroot juice on sprint interval cycling exercise performance. Twenty-four participants (male or female) will undertake a randomized, crossover trial where they consume either (i) an isotonic beetroot juice drink containing nitrate (12.9 mmol) for 5 days or (ii) an isotonic sports drink (no nitrate) for 5 days. Drinks will be equivalent in isotonicity and colored the same so that participants and investigators will be blinded to each trial. Randomization and drink allocation will be completed by a third person not associated with the study team. On Day 5 of each trial, participants will come to the laboratory and 2 hours after consuming their allocated drink will complete a 6 bouts of sprint interval exercise (10 second all-out maximal sprints interspersed with 50 seconds rest). Power output (peak and mean) during cycling sprints will be measured as the primary outcome measure. Salivary measures of nitrate/nitrite will be taken at three time points over the course of the morning on Day 5 - (i) pre-drink consumption; (ii) 2 hours post-drink consumption; and (iii) post-exercise. Plasma measures of nitrate and nitrite will be taken at two time points over the course of the morning on Day 5 - (i) pre-drink consumption; and (ii) 2 hours post-drink consumption. Near infra-red spectroscopy (NIRS) will be used to measure muscle (vastus lateralis) oxygenation during cycling bouts. A Stroop test to measure inhibition (executive function) will be taken pre- and post-exercise. Sensory perception of the drink will be measured immediately post-consumption on Day 5. After a 5-day washout period participants will crossover and begin their second trial.
Study design
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Double blind (participant, investigator)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| High nitrate | Experimental | Other: Dietary Supplement: High nitrate isotonic drinkHigh nitrate isotonic drink |
| No nitrate | Active comparator | Other: Dietary Supplement: No nitrate isotonic drinkNo nitrate isotonic drink |
Who may qualify
- Ages
- 21 to 40 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (8 listed)
- Age: 21 - 40 years
- Healthy and injury-free (e.g., no cardiovascular disease, diabetes mellitus, orthopaedic impairment that interferes with moderate-to-vigorous exercise)
- Blood pressure < 130/90 mmHg
- Be physically active: Engaging in a minimum of 150 minutes of moderate-intensity exercise or 75 minutes of vigorous-intensity physical activity each week.
- Recreational cyclist (Minimum 1 hour cycling per week).
Key exclusion criteria (10 listed)
- Any allergies to beetroot or isotonic drink.
- Asthma
- Any diagnosed form of cardiometabolic disease (heart disease, stroke, peripheral vascular disease, diabetes, metabolic syndrome, high blood pressure).
- Any symptoms contraindicating exercise testing (e.g. chest pains).
- Any balance or dizziness problems.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Dietary Supplement: High nitrate isotonic drinkHigh nitrate (12.9 mmol) drink with carbohydrate and minerals | Other | High nitrate |
| Dietary Supplement: No nitrate isotonic drinkCarbohydrate and mineral isotonic drink | Other | No nitrate |
Primary outcome
Power output during maximal sprint cycling
Time frame: 2 hours after drink ingestion
Peak and mean power output
5 secondary outcomes
Salivary nitrate
Time frame: Pre-drink, 2 hours post-drink and immediately post-exercise (2.5 hours post-drink)
Plasma nitrate and nitrite
Time frame: Pre-drink and 2 hours post-drink
Muscle oxygenation
Time frame: During exercise (2-2.5 hours post drink)
Sensory evaluation (Acceptance and Preference Test Evaluation by Meilgaard et al 2007)
Time frame: Immediately post-drink (1 minute post-drink)
Stroop test
Time frame: Immediately pre- (2 hours post-drink) and immediately post-exercise (2.5 hours post drink)
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 13 Apr 2025.
- Individual participant dataSharing planned: Yes. All published data will be placed into the Data Repository of Nanyang Technological University National Institute of Education for public access upon publication.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Dietary Supplement: High nitrate isotonic drink and Dietary Supplement: No nitrate isotonic drink in people with Exercise Performance, run by Nanyang Technological University at 1 site in Singapore.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 8 inclusion and 10 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 13 Apr 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Singapore. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Power output during maximal sprint cycling (time frame: 2 hours after drink ingestion). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 13 Apr 2025.