Endothenial Dysfunction6000mNCT06890741
Inflamation, Gut Leakage, Iron Status After 6000m Ergometer Test in Elite Rowers.
An observational study of Endothenial Dysfunction and Iron Status, run by Poznan University of Physical Education at 1 site in Poland.
Trial summary
The influence of a 6000m ergometer test on the markers of gut injury, endotoxemia, iron status, stress and inflammation in elite rowers. Read more about this study
- Who is it for?
- Ages 18 to 24 yearsHealthy volunteers can take part
- Conditions the study is for
- Endothenial Dysfunction, Iron Status, Oxidative Stress
- What is being tested?
- exercise test
- How many people
- 21 participants
- Where
- 1 sitePoland
- Study dates
- 1 Nov 2023to 10 Nov 2023
- Phase
- Observational
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 24 years
- All sexes
- Healthy volunteers accepted
- Adult
Study population: Male rowers age 18 - 24 years.
Who may be able to join
- minimum five years of training,
- five training sessions per week minimum,
- total training time minimum of 240 minutes,
- membership in the Polish Rowing Team,
- finishing the 6000-meter ergometer test.
Who cannot join
- antibiotic therapy within the last three months,
- probiotics and prebiotics within the last three months,
- dietary regime,
- gastrointestinal diseases.
Trial locations and contacts
1 site in 1 country.
Poland1 site
Poznan University of Physical Education
Gorzów Wielkopolski, Lubusz Voivodeship, 66-400
About this study
Official title: Influance of 6000m Ergometer Test on Inflammatory, Permeability Markers and Iron Status.
The influence of a 6000m ergometer test on the markers of gut injury, endotoxemia, iron status, stress and inflammation in elite rowers.
Read the detailed description
The principal objective of our study was to assess gut permeability after completing a 6000-meter rowing test. The study was performed at the beginning of a competitive training phase. Eighteen elite Polish Rowing Team rowers participated in the study after applying the inclusion/exclusion criteria. The participants performed a 6000-meter ergometer test. Blood samples were taken before the test, after exercise, and after 1 hour of restitution. Parameters, such as I-FABP (intestinal fatty acid binding protein), LPS (lipopolysaccharide), LBP (lipopolysaccharide binding protein), claudin3, ferritin, transferrin, hepcidin, IL-6 (Interleukin-6), HO-1 (Heme Oxygenase 1), and ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) were determined using appropriate biochemical tests.
- Conditions
- Endothenial Dysfunction, Iron Status, Oxidative Stress
- Being tested
- exercise test
- Keywords
- endotoxin, gut permeability, gastrointestinal function, iron status, oxidative stress
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
exercise testOther
Participant perforemed 6000m ergometer test.
| Study group | Role | What they receive |
|---|---|---|
| elite rowers | Not described Receives: Other: exercise test |
What is being measured
The primary outcome is the main question the study is designed to answer.
I-FABP (intestinal fatty acid binding protein)to measure epithelial wall injury
I-FABP concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays
Measured: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LBP (lipopolysaccharide binding protein) to measure endotoxin
LBP concentration measured in blood [ug/ml] using commercially available enzyme-linked immunosorbent assays
Measured: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Claudin 3 to measure tight junction leakage
claudin 3 concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays
Measured: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
LPS (lipopolysaccharide) to measure endotoxin
LPS concentration measured in blood [EU/Ll] using commercially available enzyme-linked immunosorbent assays
Measured: Blood samples was taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period
Lactic acid to measure fatigue after the race
Lactate concentration measured in capillary blood from earlobe [mmol/L] using a commercially available kit (Diaglobal, Berlin, Germany)
Measured: Blood samples was taken from earlobe at two-time points: before each stress test (after overnight fasting), one minute after the end of the test.
Ferritin to measure iron status
Ferritin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays
Measured: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
Transferrin to measure iron status
Transferrin concentration measured in blood [mg/ml] using commercially available enzyme-linked immunosorbent assays
Measured: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
Hepcidin to measure iron status
Hepcidin concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays
Measured: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
IL-6 (Interleukin-6) to measure inflammation
IL-6 concentration measured in blood [ng/l] using commercially available enzyme-linked immunosorbent assays.
Measured: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
HO-1 (Heme Oxygenase 1) to measure oxidative stress
HO-1 concentration measured in blood [ng/ml] using commercially available enzyme-linked immunosorbent assays
Measured: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
ACSL4 (Long-chain-fatty-acid-CoA ligase 4 ) to measure oxidative stress
ACSL4 concentration measured in blood [pg/ml] using commercially available enzyme-linked immunosorbent assays.
Measured: Blood samples will be taken from the cubital vein at three-time points: before each stress test (after overnight fasting), one minute after the end of the test, and after a 1-hour recovery period.
Secondary outcomes (11)
energy
energy [kcal ]of food intake measured by 24h dietary recall
Measured: day before test, in the morning before the test
protein
protein [g ]of food intake measured by 24h dietary recall
Measured: day before test, in the morning before the test
carbohydrate
carbohydrate [g ]of food intake measured by 24h dietary recall
Measured: day before test, in the morning before the test
fat
fat [g ]of food intake measured by 24h dietary recall
Measured: day before test, in the morning before the test
fiber
fiber [g ]of food intake measured by 24h dietary recall
Measured: day before test, in the morning before the test
body mass
Body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Measured: in the morning before the test
Body composition - water
% of body water measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Measured: in the morning before the test
Body composition - fat
% of body fat measured by electronic scale (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Measured: in the morning before the test
Lean body mass
Lean body mass [kg] measured by electronic scale to the nearest 0,05 kg (Tanita BC 418 MA Tanita Corporation, Tokyo, Japan).
Measured: in the morning before the test
Height
Prior to the exercise test, we measured the anthropometric parameters, including height (Seca 213 Hamburg, Deutschland) [cm]
Measured: in the morning before the test
Gastrointestinal sypthomps self reported scale
10 point scale were 10 is a outcome of the worst severity
Measured: up to 1 hours after the test
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted24 Mar 2025
- Study start1 Nov 2023
- Primary completion10 Nov 2023
- Study completion10 Nov 2023
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Endothenial DysfunctionStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Observational
- Observational model
- Cohort
- Time perspective
- Retrospective
- Enrolment
- 21 participants (actual)
Sponsor and investigators
- Lead sponsor
- Poznan University of Physical Education
- Sponsor type
- University, hospital or other
- Responsible party
- Sponsor
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT06890741
- Sponsor's protocol ID
- Rowers6000m 2023
- Acronym
- 6000m
- Status last verified
- Nov 2023
- First submitted
- 17 Mar 2025
- Last updated
- 27 Mar 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No