Water TurnoverNCT07028021
Study on Water Turnover of the Chinese Population Based on Deuterium Dilution Method
An observational study of Water Turnover and Population Water Requirements, run by Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences at 1 site in China.
Trial summary
Water is essential for sustaining life and maintaining health. The human body relies on water to transport nutrients and waste products, lubricate joints, regulate body temperature, and preserve tissue structure. Read more about this study
- Who is it for?
- Ages 18 years and olderHealthy volunteers can take part
- Conditions the study is for
- Water Turnover, Population Water Requirements, Deuterium Dilution
- How many people
- 300 participants
- Where
- 1 siteChina
- Study dates
- 6 May 2025to 30 Oct 2026
- Phase
- Observational
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 years and older
- All sexes
- Healthy volunteers accepted
- Adult, Older adult
Study population: The study population consisted of healthy individuals with stable body weight over the past year and the ability to engage in normal physical activities. Participants had no history of chronic diseases, were not taking diuretics or other medications that affect urine output, and female participants were neither pregnant nor breastfeeding.
Who may be able to join
- Stable body weight over the past year
- Ability to perform normal physical activities
Who cannot join
- History of chronic diseases such as diabetes, liver disease, kidney disease, or any other conditions that may affect fluid metabolism
- Use of diuretics or other medications that influence urine output
- Pregnancy or breastfeeding
Trial locations and contacts
1 site in 1 country. Contact the study team or a site near you to ask about taking part.
China1 site, 1 recruiting
- Recruiting
Beijing
Beijing, Beijing Municipality
Yukun Liu
About this study
Water is essential for sustaining life and maintaining health. The human body relies on water to transport nutrients and waste products, lubricate joints, regulate body temperature, and preserve tissue structure. International research has shown that losing just 2% of body weight through water loss can impair physiological functions and reduce response capacity. Adequate water intake plays a crucial protective role in health: it helps regulate body weight, lowers the risk of chronic diseases such as cardiovascular and cerebrovascular conditions, and supports cognitive function.
However, the common recommendation to drink eight cups of water per day lacks strong scientific evidence. Most national hydration guidelines are based on average population intake, and personalized water intake recommendations have yet to be fully developed.
Read more
In this study, the investigators will use the deuterium dilution technique to measure water turnover in volunteers. Body composition will be assessed using the TANITA MC-780, while a seven-day fluid intake diary will track daily water consumption. Physical activity levels will be monitored using GT3 devices, and environmental data-including altitude, temperature, and humidity-will be collected based on participants' locations.
The goal of this study is to collect comprehensive data on the physiological traits, environmental influences, and hydration needs of the Chinese population. This will allow for refinement and optimization of current prediction equations for water requirements. Ultimately, the investigators aim to develop a new, individualized water intake algorithm tailored to the Chinese population by quantifying the proportion of water intake relative to total water requirements.
Read the detailed description
The following assessments will be conducted with the volunteers:
Height Measurement: Height will be measured using a Seca 217 stable stadiometer. Participants will be asked to remove their shoes for the measurement.
Body Composition Measurement: Body composition will be assessed using the TANITA MC-780 device. This provides individual segmental weight measurements for the right arm, left arm, trunk, right leg, and left leg. A comprehensive report will also be generated, including body weight (BW), body fat percentage (fat%), fat mass (FM), body mass index (BMI), and fat-free mass (FFM).
Body Water Measurement: Total body water will be measured using the deuterium dilution technique. Participants will arrive at the laboratory after an overnight fast and provide a baseline urine sample. They will then ingest a dose of deuterated water calculated based on their body weight. Additional urine samples will be collected three and four hours after dosing. The increase in deuterium concentration compared to baseline will be used to calculate total body water.
Physical Activity Measurement: Physical activity will be monitored using a GT3 accelerometer, worn near the hip for seven consecutive days. The device should be removed during bathing or swimming. The first day of data will be excluded, as well as any day with less than 12 hours of wear time. Valid data must include at least two weekdays and two weekend days.
Water Intake Measurement: Water intake will be tracked using a seven-day liquid intake diary. Participants will be provided with a form to record the type, time, and amount of liquid consumed across eight specific time intervals each day. Detailed instructions will be provided. Continuous recording for the full seven-day period is required.
Environmental Data Collection: Environmental temperature and relative humidity will be recorded using an iButton device. The average altitude of each participant's city of residence will be determined using GPS-based altitude software.
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
| Study group | Role | What they receive |
|---|---|---|
| Volunteers from Beijing | A total of 50 local volunteers will be recruited in Beijing to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). | |
| Volunteers from Shenzhen | A total of 50 local volunteers will be recruited in Shenzhen to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). | |
| Volunteers from Shenyang | A total of 50 local volunteers will be recruited in Shenyang to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). | |
| Volunteers from Yantai | A total of 50 local volunteers will be recruited in Yantai to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). | |
| Volunteers from Xining | A total of 50 local volunteers will be recruited in Xining to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). | |
| Volunteers from Wenzhou | A total of 50 local volunteers will be recruited in Wenzhou to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). |
What is being measured
The primary outcome is the main question the study is designed to answer.
Height
Height in meters (m) will be measured using the SECA 217 stable stadiometer. Subjects will be measured without shoes.
Measured: Height measurement will be conducted on the first day of the study for each volunteer and will take approximately 2 minutes.
Body weight
Body weight in kilograms (kg) will be measured using the TANITA MC-780 device. Participants will be barefoot and dressed in light clothing during the measurement.
Measured: Body weight will be conducted on the first day of the study for each volunteer and will take approximately 5 minutes.
Body composition
The TANITA (TANITA MC-780) device will be used to measure fat mass (in kilograms, kg) as a primary indicator of body composition. Participants will be asked to remove all metal accessories and wear light clothing during the measurement.
Measured: Body composition ( fat mass )will be conducted on the first day of the study for each volunteer and will take approximately 5 minutes.
Body water
Body water in kilograms will be measured by deuterium dilution. Subjects will attend the lab after an overnight fast and provide a baseline urine sample. They will then drink a dose of deuterated water based on their body weight. Urine samples will be collected at 4 hours after dosing, and 7 days after dosing, to measure the level of deuterium isotope in the body relative to the baseline. The increase in deuterium concentration allows for the calculation of total body water based on the dilution principle.
Measured: Deuterium dilution technology will be initiated at the start of the volunteer's research visit, with each volunteer undergoing a one-week data collection period .
Physical Activity
Physical activity (in vector magnitude counts) of the participants will be recorded using GT3X accelerometer worn near the hip for a consecutive period of 7 days. The monitor should not be worn while bathing or swimming. The first day is discarded along with any day where the wear time is less than 12 hours. For a valid measure the goal is to get 2 weekday and 2 weekend days.
Measured: Physical activity will be initiated at the start of the volunteer's research visit, with each volunteer undergoing a one-week data collection period.，and will be used in subsequent data analyses.
Water Intake Measurement
Water intake will be recorded using a seven-day liquid intake diary. A standardized form will be provided to the volunteers, where they will record the type, time, and amount (in milliliters, mL) of liquid consumed across approximately four specific time periods each day. Detailed recording standards and instructions will be included in the form.
Measured: Water intake will be initiated at the start of the volunteer's research visit, with each volunteer undergoing a one-week data collection period, and will be used in subsequent data analyses.
Environmental temperature
The iButton (DS1921G) will be used to assess the subject's exposure to temperature (in Degrees Celsius) by measuring both indoor and outdoor temperatures. The devices will be placed on the subject's hand bag or clothes, the indoor wall of the home and workplace, and the exterior building surface.
Measured: Environmental temperature exposure will be monitored for one week beginning at the start of the volunteer's research visit
Average residential altitude of participant
The average altitude (in meters) of the participant's residential city will be determined using GPS-based altitude software.
Measured: The average altitude will be recorded at the start of the volunteer's participation in the study and will take approximately 2 minutes.
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted19 Jun 2025
- Study start6 May 2025
- Primary completion30 Jun 2026 (estimated)
- Study completion30 Oct 2026 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Water TurnoverStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Observational
- Observational model
- Cohort
- Time perspective
- Cross-sectional
- Enrolment
- 300 participants (estimated)
Sponsor and investigators
- Lead sponsor
- Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Xueying Zhang, Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07028021
- Sponsor's protocol ID
- SIAT-IRB-241115-H0925
- Status last verified
- Jun 2025
- First submitted
- 3 Jun 2025
- Last updated
- 19 Jun 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Study at a glance
From the registry record, last updated by the sponsor on 19 Jun 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Study on Water Turnover of the Chinese Population Based on Deuterium Dilution Method
- ClinicalTrials.gov ID
- NCT07028021
- Acronym / sponsor ID
- SIAT-IRB-241115-H0925
- Study type
- Observational
- Recruitment status
- Recruiting
- Enrolment
- 300 participants (estimated)
- Lead sponsor
- Shenzhen Institutes of Advanced Technology ,Chinese Academy of SciencesUniversity, hospital or other
- Study start
- 6 May 2025 (actual)
- Primary completion
- 30 Jun 2026 (estimated)
- Study completion
- 30 Oct 2026 (estimated)
- First posted
- 19 Jun 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Water is essential for sustaining life and maintaining health. The human body relies on water to transport nutrients and waste products, lubricate joints, regulate body temperature, and preserve tissue structure. International research has shown that losing just 2% of body weight through water loss can impair physiological functions and reduce response capacity. Adequate water intake plays a crucial protective role in health: it helps regulate body weight, lowers the risk of chronic diseases such as cardiovascular and cerebrovascular conditions, and supports cognitive function.
However, the common recommendation to drink eight cups of water per day lacks strong scientific evidence. Most national hydration guidelines are based on average population intake, and personalized water intake recommendations have yet to be fully developed.
In this study, the investigators will use the deuterium dilution technique to measure water turnover in volunteers. Body composition will be assessed using the TANITA MC-780, while a seven-day fluid intake diary will track daily water consumption. Physical activity levels will be monitored using GT3 devices, and environmental data-including altitude, temperature, and humidity-will be collected based on participants' locations.
The goal of this study is to collect comprehensive data on the physiological traits, environmental influences, and hydration needs of the Chinese population. This will allow for refinement and optimization of current prediction equations for water requirements. Ultimately, the investigators aim to develop a new, individualized water intake algorithm tailored to the Chinese population by quantifying the proportion of water intake relative to total water requirements.
Detailed description
The following assessments will be conducted with the volunteers:
Height Measurement: Height will be measured using a Seca 217 stable stadiometer. Participants will be asked to remove their shoes for the measurement.
Body Composition Measurement: Body composition will be assessed using the TANITA MC-780 device. This provides individual segmental weight measurements for the right arm, left arm, trunk, right leg, and left leg. A comprehensive report will also be generated, including body weight (BW), body fat percentage (fat%), fat mass (FM), body mass index (BMI), and fat-free mass (FFM).
Body Water Measurement: Total body water will be measured using the deuterium dilution technique. Participants will arrive at the laboratory after an overnight fast and provide a baseline urine sample. They will then ingest a dose of deuterated water calculated based on their body weight. Additional urine samples will be collected three and four hours after dosing. The increase in deuterium concentration compared to baseline will be used to calculate total body water.
Physical Activity Measurement: Physical activity will be monitored using a GT3 accelerometer, worn near the hip for seven consecutive days. The device should be removed during bathing or swimming. The first day of data will be excluded, as well as any day with less than 12 hours of wear time. Valid data must include at least two weekdays and two weekend days.
Water Intake Measurement: Water intake will be tracked using a seven-day liquid intake diary. Participants will be provided with a form to record the type, time, and amount of liquid consumed across eight specific time intervals each day. Detailed instructions will be provided. Continuous recording for the full seven-day period is required.
Environmental Data Collection: Environmental temperature and relative humidity will be recorded using an iButton device. The average altitude of each participant's city of residence will be determined using GPS-based altitude software.
Study design
- Observational model
- Cohort
- Time perspective
- Cross-sectional
- Number of arms
- 6
| Arm | Type | Receives |
|---|---|---|
| Volunteers from Beijing | – | –A total of 50 local volunteers will be recruited in Beijing to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). |
| Volunteers from Shenzhen | – | –A total of 50 local volunteers will be recruited in Shenzhen to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). |
| Volunteers from Shenyang | – | –A total of 50 local volunteers will be recruited in Shenyang to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). |
| Volunteers from Yantai | – | –A total of 50 local volunteers will be recruited in Yantai to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). |
| Volunteers from Xining | – | –A total of 50 local volunteers will be recruited in Xining to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). |
| Volunteers from Wenzhou | – | –A total of 50 local volunteers will be recruited in Wenzhou to participate in the study. The study procedures will include height measurement, body composition analysis, body water turnover assessment, water intake documentation, physical activity level monitoring, and evaluation of environmental factors (temperature, humidity, and altitude). |
Who may qualify
- Ages
- 18 years and older
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult, Older adult
- Study population
- The study population consisted of healthy individuals with stable body weight over the past year and the ability to engage in normal physical activities. Participants had no history of chronic diseases, were not taking diuretics or other medications that affect urine output, and female participants were neither pregnant nor breastfeeding.
Key inclusion criteria (2 listed)
- Stable body weight over the past year
- Ability to perform normal physical activities
Key exclusion criteria (3 listed)
- History of chronic diseases such as diabetes, liver disease, kidney disease, or any other conditions that may affect fluid metabolism
- Use of diuretics or other medications that influence urine output
- Pregnancy or breastfeeding
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Primary outcome
Height
Time frame: Height measurement will be conducted on the first day of the study for each volunteer and will take approximately 2 minutes.
Height in meters (m) will be measured using the SECA 217 stable stadiometer. Subjects will be measured without shoes.
Body weight
Time frame: Body weight will be conducted on the first day of the study for each volunteer and will take approximately 5 minutes.
Body weight in kilograms (kg) will be measured using the TANITA MC-780 device. Participants will be barefoot and dressed in light clothing during the measurement.
Body composition
Time frame: Body composition ( fat mass )will be conducted on the first day of the study for each volunteer and will take approximately 5 minutes.
The TANITA (TANITA MC-780) device will be used to measure fat mass (in kilograms, kg) as a primary indicator of body composition. Participants will be asked to remove all metal accessories and wear light clothing during the measurement.
Body water
Time frame: Deuterium dilution technology will be initiated at the start of the volunteer's research visit, with each volunteer undergoing a one-week data collection period .
Body water in kilograms will be measured by deuterium dilution. Subjects will attend the lab after an overnight fast and provide a baseline urine sample. They will then drink a dose of deuterated water based on their body weight. Urine samples will be collected at 4 hours after dosing, and 7 days after dosing, to measure the level of deuterium isotope in the body relative to the baseline. The increase in deuterium concentration allows for the calculation of total body water based on the dilution principle.
Physical Activity
Time frame: Physical activity will be initiated at the start of the volunteer's research visit, with each volunteer undergoing a one-week data collection period.，and will be used in subsequent data analyses.
Physical activity (in vector magnitude counts) of the participants will be recorded using GT3X accelerometer worn near the hip for a consecutive period of 7 days. The monitor should not be worn while bathing or swimming. The first day is discarded along with any day where the wear time is less than 12 hours. For a valid measure the goal is to get 2 weekday and 2 weekend days.
Water Intake Measurement
Time frame: Water intake will be initiated at the start of the volunteer's research visit, with each volunteer undergoing a one-week data collection period, and will be used in subsequent data analyses.
Water intake will be recorded using a seven-day liquid intake diary. A standardized form will be provided to the volunteers, where they will record the type, time, and amount (in milliliters, mL) of liquid consumed across approximately four specific time periods each day. Detailed recording standards and instructions will be included in the form.
Environmental temperature
Time frame: Environmental temperature exposure will be monitored for one week beginning at the start of the volunteer's research visit
The iButton (DS1921G) will be used to assess the subject's exposure to temperature (in Degrees Celsius) by measuring both indoor and outdoor temperatures. The devices will be placed on the subject's hand bag or clothes, the indoor wall of the home and workplace, and the exterior building surface.
Average residential altitude of participant
Time frame: The average altitude will be recorded at the start of the volunteer's participation in the study and will take approximately 2 minutes.
The average altitude (in meters) of the participant's residential city will be determined using GPS-based altitude software.
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 19 Jun 2025.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
An observational study of Water Turnover and Population Water Requirements, run by Shenzhen Institutes of Advanced Technology ,Chinese Academy of Sciences at 1 site in China.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 2 inclusion and 3 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: looking for participants now, as of the sponsor's last update on 19 Jun 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in China. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
Contact Xueying Zhang (+8618201296155, zhangxy@siat.ac.cn), or a site near the patient. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Height (time frame: Height measurement will be conducted on the first day of the study for each volunteer and will take approximately 2 minutes.). The study lists 8 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 19 Jun 2025.