HealthyNCT07104734
Evaluation of the Ergogenic Properties of Hesperetin on Exercise-induced Fatigue
A study testing Hesperetin (Hesperetin trial) in people with Healthy and Athletes, run by China Medical University Hospital at 1 site in Taiwan.
Trial summary
Twenty young, healthy, physically active men and women (12~30 years) did a pre-post trial randomized into placebo or HST trials (500 mg/d for two weeks). HST supplementation enhanced endurance judo-specific performance and effectively attenuated exercise-induced fatigue, oxidative stress, inflammatory response, and… Read more about this study
- Who is it for?
- Ages 12 to 30 yearsHealthy volunteers can take part
- What is being tested?
- Hesperetin (Hesperetin trial)
- Type of treatment
- Pill or oral medicine
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 20 participants
- Where
- 1 siteTaiwan
- Study dates
- 3 Sep 2024to 30 Jun 2025
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 12 to 30 years
- All sexes
- Healthy volunteers accepted
- Child, Adult
Who may be able to join
- Judo athletes aged 12 to 30 years with at least 5 years of judo training experience.
Who cannot join
- All participants must be free from chronic conditions such as heart disease and hypertension, and must not have experienced any serious lower limb musculoskeletal injuries within the past six months.
Trial locations and contacts
1 site in 1 country.
About this study
Official title: Evaluation of the Ergogenic Properties of Citrus Flavonoid Hesperetin: Investigating Exercise-induced Fatigue, Oxidative Stress, Inflammation, Muscle Damage Mechanisms, and Judo-specific Performance
Twenty young, healthy, physically active men and women (12~30 years) did a pre-post trial randomized into placebo or HST trials (500 mg/d for two weeks). HST supplementation enhanced endurance judo-specific performance and effectively attenuated exercise-induced fatigue, oxidative stress, inflammatory response, and muscle damage.
Read the detailed description
Participants in this study will be assigned identification numbers and randomly allocated to either the hesperetin (Hst) trial or the placebo trial. Seven days before the formal exercise performance test, all participants will undergo a maximal oxygen uptake (VO₂max) test using a cycle ergometer. The judo simulation consists of four consecutive 4-minute matches, each separated by a 30-minute rest. Participants will supplement with either placebo or Hst (500 mg/day) for 2 weeks. They are required to fast for 12 hours before the test day. On the experimental day, participants consume a light meal (300 kcal) and intake of either the placebo or Hst capsule, then rest for 1 hour. Afterward, participants perform a 15-minute judo-specific warm-up. All participants are required to complete all three matches. To standardize the judo simulation, opponents will be matched so that weight differences do not exceed 10%. Between the 30-minute rest time, blood samples will be collected and judo-specific physical performance will be evaluated. Immediately following the recovery period of the fourth match, the Special Judo Fitness Test (SJFT) will be evaluated. Judo-specific physical performance assessments will include grip strength, Counter Movement Jump (CMJ), and 10-second Wingate test.
- Being tested
- Placebo (Placebo trial), Hesperetin (Hesperetin trial)
- Keywords
- Hesperetin, judo, exercise performance
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Placebo (Placebo trial)Other
The Placebo capsule supplement was taken for 2 weeks (2 capsules per day)
Hesperetin (Hesperetin trial)Other
The Hesperetin capsule supplement was taken for 2 weeks, with a daily dosage of 500 mg of Hesperetin (equivalent to 2 capsules per day, each containing 250 mg of Hesperetin and 50 mg Pycnogenol®).
| Study group | Role | What they receive |
|---|---|---|
| Placebo | Placebo comparator | Containing edible yellow No. 4, edible yellow No. 5, sucrose, silica, talc, oxidized starch, gelatin, magnesium stearate, and palm wax. Receives: Other: Placebo (Placebo trial); Other: Hesperetin (Hesperetin trial) |
| Hesperetin | Experimental | Each capsule containing 250 mg of Hesperetin Receives: Other: Placebo (Placebo trial); Other: Hesperetin (Hesperetin trial) |
What is being measured
The primary outcome is the main question the study is designed to answer.
Special Judo Fitness Test (SJFT)
The Special Judo Fitness Test (SJFT) was developed by Sterkowicz (1995) and has been validated through multiple studies demonstrating its objectivity, reliability, and validity. The SJFT consists of a task in which the athlete must throw a partner as many times as possible using maximum effort. The test is divided into three intervals: 15 seconds, 30 seconds, and 30 seconds, with 10-second rest periods in between. During each interval, the subject is verbally encouraged to perform as many throws as possible using the ippon-seoi-nage technique, with the throwing partners positioned 6 meters apart. After the test, heart rate (HR) is recorded immediately and again one minute post-exercise. The SJFT index is calculated based on the total number of throws and heart rate data using the following formula: SJFT Index = (Final HR [bpm] + HR at 1 minute post-test [bpm] / Total Number of Throws.
Measured: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Upper Body Grip Strength Test
Grip strength will be measured using a handgrip dynamometer (SHM-18) to assess upper limb muscle strength. First, the examiner instructs the participant to let both arms hang naturally by the sides of the body. The grip span of the dynamometer is then adjusted so that the second knuckle of the index finger forms a right angle when gripping the handle. Once the correct posture is confirmed, the test begins.Grip strength will be measured on both the dominant and non-dominant hands. After completing one side and recording the value, the device is reset to zero, and the participant rests for 30 seconds before testing the other hand. During the test, the dynamometer must not come into contact with the body.Each hand is tested twice, and the maximum value (measured in kilograms) from the two trials on each side is recorded as the reference score.
Measured: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Lower Limb Explosive Power - Countermovement Jump (CMJ)
The CMJ is used to assess lower limb maximal strength, explosive power, and speed. The CMJ test will be conducted using a sports performance measurement system to evaluate participants' lower limb explosive power. Under the guidance of the tester, participants will stand upright with knees fully extended, feet approximately shoulder-width apart, and hands placed on their waist to restrict arm swing, isolating lower limb performance.Participants will perform two maximal-effort jumps, with a 1-minute rest between attempts. The best jump height will be recorded as the test result. During the test, upon the tester's "start" command, participants will perform a rapid downward (countermovement) action to a self-selected depth, immediately followed by a maximal vertical jump.
Measured: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
10-Second Anaerobic Power Test
The test will be conducted using a stationary Monark 894E cycle ergometer. During the recovery period after each simulated judo match, participants will begin pedaling at a cadence of 60 rpm. The tester will then count down "3, 2, 1," and upon the "Go" command, participants will pedal at maximal effort and speed for 10 seconds. After completing the 10-second sprint, participants will continue pedaling at 60 rpm for a cooldown period as instructed by the tester to prevent dizziness caused by sudden cessation of exercise.Each participant will complete one 10-second anaerobic power test during the recovery period of each judo match. Anaerobic power indicators include peak power (occurring within the first 3 to 5 seconds of the test), total power output (W), and relative power output normalized to body weight (W/kg). The resistance load for the Wingate anaerobic power test is set at body weight (kg) × 0.75.
Measured: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Assessment of the profile of mood state (POMS)
POMS brief was chosen for this study to evaluate the mood of individuals. After the TTE, participants completed the form, and their responses were analyzed using simple statistics. The results were shown in six dimensions, one positive: (1) vigor; and five negatives: (2) tension, (3) depression, (4) anger, (5) fatigue and (6) confusion.
Measured: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Clinical Biochemistry of muscle damage biomarkers
Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1~3), and immediately (E) after the Special Judo Fitness Test. The concentrations of muscle damage markers creatine kinase (CK), myoglobin, and high-sensitivity C-reactive protein (hs-CRP) were measured using commercial assay kits (San Diego, CA, USA) and enzyme-linked immunosorbent assay (ELISA) with a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 450 nm, 505 nm, and 572 nm, respectively. The concentrations of CK, myoglobin, and hs-CRP in serum samples were determined based on standard curves.
Measured: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Clinical Biochemistry of oxidative stress
Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1~3), and immediately (E) after the Special Judo Fitness Test. The oxidative stress markers, including total antioxidant capacity (TAC), malondialdehyde (MDA), and thiobarbituric acid reactive substances (TBARS), were measured using commercial assay kits (Sigma, St.) and enzyme-linked immunosorbent assay (ELISA) with a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 532 nm and 570 nm, and the concentrations of TAC, MDA, and TBARS in serum samples were determined based on standard curves.
Measured: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Clinical Biochemistry of metabolic markers and safety assessment
Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1~3), and immediately (E) after the Special Judo Fitness Test. The metabolic markers glucose, non-esterified fatty acid (NEFA), and triglycerides (TG) were analyzed using commercial assay kits (WAKO NEFA, Germany) and measured with a fully automated biochemical analyzer (7020, Hitachi Science Systems, Ltd., Ibaraki, Japan). For safety assessment, GOP and GPT levels were determined using enzymatic analysis with commercial assay kits (Sigma, St.) and enzyme-linked immunosorbent assay (ELISA) on a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 595 nm, and the concentrations of GOP and GPT in serum samples were calculated based on standard curves.
Measured: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Clinical Biochemistry of cytokines
Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1~3), and immediately (E) after the Special Judo Fitness Test. The cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were measured using commercial assay kits (Bio-Legend Inc., No. 430207, San Diego, CA, USA) and enzyme-linked immunosorbent assay (ELISA) with a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was read at wavelengths of 450 nm and 570 nm, and the concentrations of IL-6 and TNF-α in serum samples were determined based on standard curves.
Measured: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Clinical Biochemistry of stress markers
Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1~3), and immediately (E) after the Special Judo Fitness Test. The stress markers catecholamine, cortisol, and testosterone were analyzed using commercial assay kits (San Diego, CA, USA) and measured with an enzyme-linked immunosorbent assay (ELISA) reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 450 nm, 505 nm, and 572 nm, respectively. The concentrations of catecholamine, cortisol, and testosterone in serum samples were determined based on standard curves.
Measured: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted5 Aug 2025
- Study start3 Sep 2024
- Primary completion30 Jun 2025
- Study completion30 Jun 2025
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for HealthyStudies of the same condition, open and under way.
- More trials run by China Medical University HospitalThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Model description
- This study recruited 20 healthy male and female participants aged 12 to 30 years with at least 5 years of judo training experience to participate in a single-blind, pre-post test trial. Participants will be randomly assigned to either the hesperetin (Hst) group or the placebo group. All participants must be free from chronic conditions such as heart disease and hypertension, and must not have experienced any serious lower limb musculoskeletal injuries within the past six months. Throughout the study period, participants will be required to maintain their regular daily routines and will be prohibited from consuming any other nutritional supplements or alcohol.
- Masking
- Quadruple blind
- Who is masked
- Participant, Care provider, Investigator, Outcomes assessor
- Enrolment
- 20 participants (actual)
Sponsor and investigators
- Lead sponsor
- China Medical University Hospital
- Sponsor type
- University, hospital or other
- Collaborators
- China Medical University, Taiwan
- Responsible party
- Sponsor
- Investigators
- Jung-Piao Tsao, Study chair, China Medical University, China
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07104734
- Sponsor's protocol ID
- CMUH113-REC2-012
- Status last verified
- Jul 2025
- First submitted
- 29 Jul 2025
- Last updated
- 5 Aug 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Study at a glance
From the registry record, last updated by the sponsor on 5 Aug 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Evaluation of the Ergogenic Properties of Citrus Flavonoid Hesperetin: Investigating Exercise-induced Fatigue, Oxidative Stress, Inflammation, Muscle Damage Mechanisms, and Judo-specific Performance
- ClinicalTrials.gov ID
- NCT07104734
- Acronym / sponsor ID
- CMUH113-REC2-012
- Keywords
- Hesperetin, judo, exercise performance
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 20 participants (actual)
- Lead sponsor
- China Medical University HospitalUniversity, hospital or other
- Collaborators
- China Medical University, Taiwan
- Study start
- 3 Sep 2024 (actual)
- Primary completion
- 30 Jun 2025 (actual)
- Study completion
- 30 Jun 2025 (actual)
- First posted
- 5 Aug 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Twenty young, healthy, physically active men and women (12~30 years) did a pre-post trial randomized into placebo or HST trials (500 mg/d for two weeks). HST supplementation enhanced endurance judo-specific performance and effectively attenuated exercise-induced fatigue, oxidative stress, inflammatory response, and muscle damage.
Detailed description
Participants in this study will be assigned identification numbers and randomly allocated to either the hesperetin (Hst) trial or the placebo trial. Seven days before the formal exercise performance test, all participants will undergo a maximal oxygen uptake (VO₂max) test using a cycle ergometer. The judo simulation consists of four consecutive 4-minute matches, each separated by a 30-minute rest. Participants will supplement with either placebo or Hst (500 mg/day) for 2 weeks. They are required to fast for 12 hours before the test day. On the experimental day, participants consume a light meal (300 kcal) and intake of either the placebo or Hst capsule, then rest for 1 hour. Afterward, participants perform a 15-minute judo-specific warm-up. All participants are required to complete all three matches. To standardize the judo simulation, opponents will be matched so that weight differences do not exceed 10%. Between the 30-minute rest time, blood samples will be collected and judo-specific physical performance will be evaluated. Immediately following the recovery period of the fourth match, the Special Judo Fitness Test (SJFT) will be evaluated. Judo-specific physical performance assessments will include grip strength, Counter Movement Jump (CMJ), and 10-second Wingate test.
Study design
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Quadruple blind (participant, care provider, investigator, outcomes assessor)
- Number of arms
- 2
- Model description
- This study recruited 20 healthy male and female participants aged 12 to 30 years with at least 5 years of judo training experience to participate in a single-blind, pre-post test trial. Participants will be randomly assigned to either the hesperetin (Hst) group or the placebo group. All participants must be free from chronic conditions such as heart disease and hypertension, and must not have experienced any serious lower limb musculoskeletal injuries within the past six months. Throughout the study period, participants will be required to maintain their regular daily routines and will be prohibited from consuming any other nutritional supplements or alcohol.
| Arm | Type | Receives |
|---|---|---|
| Placebo | Placebo comparator | Other: Placebo (Placebo trial), Other: Hesperetin (Hesperetin trial)Containing edible yellow No. 4, edible yellow No. 5, sucrose, silica, talc, oxidized starch, gelatin, magnesium stearate, and palm wax. |
| Hesperetin | Experimental | Other: Placebo (Placebo trial), Other: Hesperetin (Hesperetin trial)Each capsule containing 250 mg of Hesperetin |
Who may qualify
- Ages
- 12 to 30 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Child, Adult
Key inclusion criteria (1 listed)
- Judo athletes aged 12 to 30 years with at least 5 years of judo training experience.
Key exclusion criteria (1 listed)
- All participants must be free from chronic conditions such as heart disease and hypertension, and must not have experienced any serious lower limb musculoskeletal injuries within the past six months.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Placebo (Placebo trial)The Placebo capsule supplement was taken for 2 weeks (2 capsules per day) | Other | Hesperetin, Placebo |
| Hesperetin (Hesperetin trial)The Hesperetin capsule supplement was taken for 2 weeks, with a daily dosage of 500 mg of Hesperetin (equivalent to 2 capsules per day, each containing 250 mg of Hesperetin and 50 mg Pycnogenol®). | Other | Hesperetin, Placebo |
Primary outcome
Special Judo Fitness Test (SJFT)
Time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
The Special Judo Fitness Test (SJFT) was developed by Sterkowicz (1995) and has been validated through multiple studies demonstrating its objectivity, reliability, and validity. The SJFT consists of a task in which the athlete must throw a partner as many times as possible using maximum effort. The test is divided into three intervals: 15 seconds, 30 seconds, and 30 seconds, with 10-second rest periods in between. During each interval, the subject is verbally encouraged to perform as many throws as possible using the ippon-seoi-nage technique, with the throwing partners positioned 6 meters apart. After the test, heart rate (HR) is recorded immediately and again one minute post-exercise. The SJFT index is calculated based on the total number of throws and heart rate data using the following formula: SJFT Index = (Final HR [bpm] + HR at 1 minute post-test [bpm] / Total Number of Throws.
Upper Body Grip Strength Test
Time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Grip strength will be measured using a handgrip dynamometer (SHM-18) to assess upper limb muscle strength. First, the examiner instructs the participant to let both arms hang naturally by the sides of the body. The grip span of the dynamometer is then adjusted so that the second knuckle of the index finger forms a right angle when gripping the handle. Once the correct posture is confirmed, the test begins.Grip strength will be measured on both the dominant and non-dominant hands. After completing one side and recording the value, the device is reset to zero, and the participant rests for 30 seconds before testing the other hand. During the test, the dynamometer must not come into contact with the body.Each hand is tested twice, and the maximum value (measured in kilograms) from the two trials on each side is recorded as the reference score.
Lower Limb Explosive Power - Countermovement Jump (CMJ)
Time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
The CMJ is used to assess lower limb maximal strength, explosive power, and speed. The CMJ test will be conducted using a sports performance measurement system to evaluate participants' lower limb explosive power. Under the guidance of the tester, participants will stand upright with knees fully extended, feet approximately shoulder-width apart, and hands placed on their waist to restrict arm swing, isolating lower limb performance.Participants will perform two maximal-effort jumps, with a 1-minute rest between attempts. The best jump height will be recorded as the test result. During the test, upon the tester's "start" command, participants will perform a rapid downward (countermovement) action to a self-selected depth, immediately followed by a maximal vertical jump.
10-Second Anaerobic Power Test
Time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
The test will be conducted using a stationary Monark 894E cycle ergometer. During the recovery period after each simulated judo match, participants will begin pedaling at a cadence of 60 rpm. The tester will then count down "3, 2, 1," and upon the "Go" command, participants will pedal at maximal effort and speed for 10 seconds. After completing the 10-second sprint, participants will continue pedaling at 60 rpm for a cooldown period as instructed by the tester to prevent dizziness caused by sudden cessation of exercise.Each participant will complete one 10-second anaerobic power test during the recovery period of each judo match. Anaerobic power indicators include peak power (occurring within the first 3 to 5 seconds of the test), total power output (W), and relative power output normalized to body weight (W/kg). The resistance load for the Wingate anaerobic power test is set at body weight (kg) × 0.75.
Assessment of the profile of mood state (POMS)
Time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
POMS brief was chosen for this study to evaluate the mood of individuals. After the TTE, participants completed the form, and their responses were analyzed using simple statistics. The results were shown in six dimensions, one positive: (1) vigor; and five negatives: (2) tension, (3) depression, (4) anger, (5) fatigue and (6) confusion.
Clinical Biochemistry of muscle damage biomarkers
Time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1~3), and immediately (E) after the Special Judo Fitness Test. The concentrations of muscle damage markers creatine kinase (CK), myoglobin, and high-sensitivity C-reactive protein (hs-CRP) were measured using commercial assay kits (San Diego, CA, USA) and enzyme-linked immunosorbent assay (ELISA) with a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 450 nm, 505 nm, and 572 nm, respectively. The concentrations of CK, myoglobin, and hs-CRP in serum samples were determined based on standard curves.
Clinical Biochemistry of oxidative stress
Time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1~3), and immediately (E) after the Special Judo Fitness Test. The oxidative stress markers, including total antioxidant capacity (TAC), malondialdehyde (MDA), and thiobarbituric acid reactive substances (TBARS), were measured using commercial assay kits (Sigma, St.) and enzyme-linked immunosorbent assay (ELISA) with a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 532 nm and 570 nm, and the concentrations of TAC, MDA, and TBARS in serum samples were determined based on standard curves.
Clinical Biochemistry of metabolic markers and safety assessment
Time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1~3), and immediately (E) after the Special Judo Fitness Test. The metabolic markers glucose, non-esterified fatty acid (NEFA), and triglycerides (TG) were analyzed using commercial assay kits (WAKO NEFA, Germany) and measured with a fully automated biochemical analyzer (7020, Hitachi Science Systems, Ltd., Ibaraki, Japan). For safety assessment, GOP and GPT levels were determined using enzymatic analysis with commercial assay kits (Sigma, St.) and enzyme-linked immunosorbent assay (ELISA) on a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 595 nm, and the concentrations of GOP and GPT in serum samples were calculated based on standard curves.
Clinical Biochemistry of cytokines
Time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1~3), and immediately (E) after the Special Judo Fitness Test. The cytokines interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were measured using commercial assay kits (Bio-Legend Inc., No. 430207, San Diego, CA, USA) and enzyme-linked immunosorbent assay (ELISA) with a microplate reader (Tecan GENios, A-5082, Austria). Absorbance was read at wavelengths of 450 nm and 570 nm, and the concentrations of IL-6 and TNF-α in serum samples were determined based on standard curves.
Clinical Biochemistry of stress markers
Time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).
Blood samples were collected at five different time points (15 min before the beginning (S), after each match (Match 1~3), and immediately (E) after the Special Judo Fitness Test. The stress markers catecholamine, cortisol, and testosterone were analyzed using commercial assay kits (San Diego, CA, USA) and measured with an enzyme-linked immunosorbent assay (ELISA) reader (Tecan GENios, A-5082, Austria). Absorbance was measured at 450 nm, 505 nm, and 572 nm, respectively. The concentrations of catecholamine, cortisol, and testosterone in serum samples were determined based on standard curves.
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 5 Aug 2025.
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Jung-Piao Tsao study chair
China Medical University, China
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Hesperetin (Hesperetin trial) in people with Healthy and Athletes, run by China Medical University Hospital at 1 site in Taiwan.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 1 inclusion and 1 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 5 Aug 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Taiwan. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Special Judo Fitness Test (SJFT) (time frame: Including for 2 days in both pre-post test trials (which lasts around 2 weeks).). The study lists 10 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 5 Aug 2025.