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Athletic PerformanceNCT07106489

Effect of Beetroot Nitrates on Different Physical Skills in Colombian Rugby Athletes

A study testing 40 meters sprint, Fitness test by bronco test and Maximal strength in people with Athletic Performance, run by Universidad Nacional de Colombia at 1 site in Colombia.

Completed

Finished. The study has ended and is not enrolling. Results may be posted on the official record.

Official record
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Trial summary

Beetroot is a source of nitrates, which have demonstrated various positive effects in sports, with little evidence in intermittent sports such as ball sports (including rugby). Therefore, the study's hypothesis is that it may also have an effect in these sports. Read more about this study

Who is it for?
Ages 18 to 40 years
Conditions the study is for
Athletic Performance
What is being tested?
40 meters sprint, Fitness test by bronco test and Maximal strength
Could I receive a placebo?
Yes, some participants may receive a placebo
How many people
10 participants
Where
1 siteColombia
Study dates
1 Feb 2025to 13 Mar 2025
Phase
Interventional
Are there results?
Yes, posted in the registrySummary in the doctors' view

Key requirements

Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.

  • Ages 18 to 40 years
  • All sexes
  • No healthy volunteers
  • Adult

Who may be able to join

  • Belong to one of the two Rugby Clubs invited to participate
  • Legal age
  • Have trained/played rugby for more than 6 months.

Who cannot join

  • Have had a sports injury in the last 6 months or during the study
  • Use of nutritional supplements during the study.
  • Use of mouthwash during the study.
  • Have health conditions that require specific medical treatment.

Trial locations and contacts

1 site in 1 country.

Colombia1 site
  • Universidad Nacional de Colombia

    Bogotá

Other trials in Colombia

About this study

Official title: Effect of Beetroot Extract Supplementation on the Athletic Performance of Trained Rugby Athletes

Beetroot is a source of nitrates, which have demonstrated various positive effects in sports, with little evidence in intermittent sports such as ball sports (including rugby). Therefore, the study's hypothesis is that it may also have an effect in these sports. For this purpose, a beetroot extract was used, a product approved by the Colombian institution in charge of product approval and registration in Colombia, the National Institute for Food and Drug Surveillance (INVIMA). This was done to determine whether it has any effect on various physical abilities required for rugby, such as speed, acceleration, strength, and fatigue perception.

Read the detailed description

Currently, nitrates (from beet juices or extracts) have gained relevance due to their vasodilatory effect, which has contributed to improving athletic performance. Their use has been studied mainly in continuous sports such as swimming, cycling, athletics, etc. However, research in intermittent sports (such as ball or combat sports) is limited and inconclusive, so further research is needed on their use and effect in these sports. Therefore, the study seeks to investigate the effect of administering beet extract for three consecutive days in rugby athletes, evaluating its effect on various physical capacities required for the specific sport, such as acceleration, speed, strength, and fitness. Due to the small number of participants, a randomization process is not possible. A representative sample of 44 is required, but only 10 are interested in participating. Therefore, the sample will be selected based on the number of people willing to participate (convenience sampling) and meeting the defined inclusion and exclusion criteria. Each athlete is subsequently characterized and evaluated by the nutritionist, who provides instructions on how to avoid consuming foods or substances that could affect the study. They will be provided with the necessary substrates three days before the physical tests, with one group receiving the beetroot substrate and the other a placebo. The following week, the groups will be double-blinded. The physical tests will be performed by a professional physiotherapist, who will apply the test protocol and use a Borg scale to assess perceived exertion. Finally, a descriptive and exploratory analysis will be performed on the collected data, using univariate tests for the mean (Student's t-test) or Wilcoxon tests, depending on the type of variable (continuous or ordinal), with a 95% confidence level. To measure the magnitude of the treatment effect on each measured variable, Cohen's d statistic will be used for those variables that required a t-test.

Being tested
beetroot juice - Placebo, 40 meters sprint, Fitness test by bronco test, Maximal strength, Borg scale
Keywords
Beetroot, Nitrates, Sports, Sports Nutrition Science

What participants receive

What participants receive. In a randomised study each person is assigned to one group by chance.

  • beetroot juice - PlaceboOther

    Participants receive the beetroot and placebo in the form of waffles to ensure the study is blinded, as the 6 mmol of nitrates used require a high concentration of beetroot, which is masked by the waffle ingredients and food coloring.

  • 40 meters sprintOther

    The participant performs a race at maximum speed with two attempts, times are taken at 10, 30 and 40 meters, to evaluate acceleration and speed, the attempt with the shortest times is the one taken for the research.

  • Fitness test by bronco testOther

    The participant runs a distance of 20, 40, and 60 meters, back and forth, 5 times at maximum speed. The variable to be measured is the total time taken to complete the test.

  • Maximal strengthOther

    Each participant performs squats and bench press to evaluate lower-body and upper-body maximal strength, thus identifying their maximum strength taken per kilogram lifted.

  • Borg scaleOther

    At the end of the tests, each participant is asked to evaluate their perception of fatigue by giving a number between 1 and 10, with 1 being the lowest level of fatigue and 10 being the highest.

Study groups in NCT07106489
Study groupRoleWhat they receive
Placebo-BeetrootOtherThe group receives the placebo the first week and the following week they receive the beetroot
Receives: Other: beetroot juice - Placebo; Other: 40 meters sprint; Other: Fitness test by bronco test; Other: Maximal strength; Other: Borg scale
Beetroot-PlaceboOtherThe group receives the beetroot the first week and the following week receives the placebo.
Receives: Other: beetroot juice - Placebo; Other: 40 meters sprint; Other: Fitness test by bronco test; Other: Maximal strength; Other: Borg scale

What is being measured

The primary outcome is the main question the study is designed to answer.

  1. Results Obtained in the Physical Tests to Evaluate Impact on Acceleration

    Physical tests are performed over two different weeks. Initially, the 40-meter sprint test is performed with two attempts, with the attempt with the shortest execution time being considered. Distances from 0 to 10 meters are used to evaluate acceleration in meters per second squared (m/s^2)

    Measured: First and second physical tests weeks

  2. Speed Results Over Distance From 0 to 30 Meters in 40 Meters Sprint Test

    In the 40-meter speed test, the time taken to travel from the start to the 30 meters is measured in meters per second (m/s)

    Measured: First and second physical tests weeks

  3. Maximum Speed Results With the Distance of 30 to 40 Meters in the 40-meter Sprint Test

    In the 40-meter sprint test, the time to cover from a distance of 30 meters to 40 meters is measured in meters per second (m/s)

    Measured: First and second physical tests weeks

  4. Speed Given by Distance From 0 to 40 Meters Sprint

    Time to cover the distance from 0 to 40 meters sprint at maximum speed in meters per second (m/s)

    Measured: First and second physical tests weeks

  5. Fitness Results From the Bronco Test

    Fitness is assessed by the time it takes to cover the 1.2 km Bronco test segmented into distances of 20, 40, and 60 meters, which are covered 5 times.

    Measured: First and second physical tests weeks

  6. Maximum Strength in the Lower-body Given by Squat

    Squats are performed technically until reaching 1 repetition maximum (1RM) meeting technical requirements.

    Measured: First and second physical tests weeks

  7. Maximum Upper-body Strength Given by Bench Press Test

    Maximum weight lifted in 1 maximum repetition in a technically correct manner in the bench press exercise

    Measured: First and second physical tests weeks

  8. Perception of Fatigue Given by the Borg Scale

    According to a score of 1 to 10, with 1 being the lowest and 10 being the highest level of fatigue, participants evaluated their perception at the end of the physical tests.

    Measured: First and second weeks at the end of the physical tests

Timeline

From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.

  1. First posted6 Aug 2025
  2. Study start1 Feb 2025
  3. Primary completion5 Mar 2025
  4. Study completion13 Mar 2025

Resources

More details

For doctors and researchers: how the study is designed, who runs it, and its registry IDs.

Study design
Study type
Interventional
Phase
Phase N/A
Primary purpose
Other
Allocation
Non-randomised
Intervention model
Crossover
Model description
The research was a quasi-experimental study using a non-randomized controlled crossover trial, minimizing the effects associated with individual athlete patterns, with each participant acting as their own control. Participants were double-blinded, dividing them into two groups. The researcher prepared placebo and beetroot waffles.
Masking
Triple blind
Who is masked
Participant, Care provider, Investigator
Enrolment
10 participants (actual)
IDs, dates and publications
ClinicalTrials.gov ID
NCT07106489
Sponsor's protocol ID
65891
Status last verified
Jun 2025
First submitted
22 Jul 2025
Last updated
12 Dec 2025
Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
Individual data sharing
Yes: The study is expected to be written and submitted to peer-reviewed, indexed journals in areas such as nutrition, exercise physiology, and public health.

Publications

  • Thompson C, Vanhatalo A, Jell H, Fulford J, Carter J, Nyman L, Bailey SJ, Jones AM. Dietary nitrate supplementation improves sprint and high-intensity intermittent running performance. Nitric Oxide. 2016 Dec 30;61:55-61. doi: 10.1016/j.niox.2016.10.006. Epub 2016 Oct 21.

    PubMed 27777094
  • Tan R, Pennell A, Karl ST, Cass JK, Go K, Clifford T, Bailey SJ, Perkins Storm C. Effects of Dietary Nitrate Supplementation on Back Squat and Bench Press Performance: A Systematic Review and Meta-Analysis. Nutrients. 2023 May 27;15(11):2493. doi: 10.3390/nu15112493.

    PubMed 37299456
  • Tan R, Cano L, Lago-Rodriguez A, Dominguez R. The Effects of Dietary Nitrate Supplementation on Explosive Exercise Performance: A Systematic Review. Int J Environ Res Public Health. 2022 Jan 11;19(2):762. doi: 10.3390/ijerph19020762.

    PubMed 35055584
  • Senefeld JW, Wiggins CC, Regimbal RJ, Dominelli PB, Baker SE, Joyner MJ. Ergogenic Effect of Nitrate Supplementation: A Systematic Review and Meta-analysis. Med Sci Sports Exerc. 2020 Oct;52(10):2250-2261. doi: 10.1249/MSS.0000000000002363.

    PubMed 32936597
  • Salem A, Ammar A, Kerkeni M, Boujelbane MA, Uyar AM, Kobel LM, Selvaraj S, Zare R, Heinrich KM, Jahrami H, Tounsi S, Zmijewski P, Schollhorn WI, Trabelsi K, Chtourou H. Short-Term Beetroot Juice Supplementation Enhances Strength, Reduces Fatigue, and Promotes Recovery in Physically Active Individuals: A Randomized, Double-Blind, Crossover Trial. Nutrients. 2025 May 19;17(10):1720. doi: 10.3390/nu17101720.

    PubMed 40431460
  • Murphy MJ, Rushing BR, Sumner SJ, Hackney AC. Dietary Supplements for Athletic Performance in Women: Beta-Alanine, Caffeine, and Nitrate. Int J Sport Nutr Exerc Metab. 2022 Feb 23;32(4):311-323. doi: 10.1123/ijsnem.2021-0176. Print 2022 Jul 1.

    PubMed 35196646
  • Miraftabi H, Avazpoor Z, Berjisian E, Sarshin A, Rezaei S, Dominguez R, Reale R, Franchini E, Samanipour MH, Koozehchian MS, Willems MET, Rafiei R, Naderi A. Effects of Beetroot Juice Supplementation on Cognitive Function, Aerobic and Anaerobic Performances of Trained Male Taekwondo Athletes: A Pilot Study. Int J Environ Res Public Health. 2021 Sep 28;18(19):10202. doi: 10.3390/ijerph181910202.

    PubMed 34639501
  • Lopez-Samanes A, Ramos-Alvarez JJ, Miguel-Tobal F, Gaos S, Jodra P, Arranz-Munoz R, Dominguez R, Montoya JJ. Influence of Beetroot Juice Ingestion on Neuromuscular Performance on Semi-Professional Female Rugby Players: A Randomized, Double-Blind, Placebo-Controlled Study. Foods. 2022 Nov 12;11(22):3614. doi: 10.3390/foods11223614.

    PubMed 36429210
  • Jones AM, Vanhatalo A, Seals DR, Rossman MJ, Piknova B, Jonvik KL. Dietary Nitrate and Nitric Oxide Metabolism: Mouth, Circulation, Skeletal Muscle, and Exercise Performance. Med Sci Sports Exerc. 2021 Feb 1;53(2):280-294. doi: 10.1249/MSS.0000000000002470.

    PubMed 32735111
  • Esen O, Karayigit R, Peart DJ. Acute beetroot juice supplementation did not enhance intermittent running performance in trained rugby players. Eur J Sport Sci. 2023 Dec;23(12):2321-2328. doi: 10.1080/17461391.2023.2230942. Epub 2023 Jul 13.

    PubMed 37394944
  • d'Unienville NMA, Blake HT, Coates AM, Hill AM, Nelson MJ, Buckley JD. Effect of food sources of nitrate, polyphenols, L-arginine and L-citrulline on endurance exercise performance: a systematic review and meta-analysis of randomised controlled trials. J Int Soc Sports Nutr. 2021 Dec 29;18(1):76. doi: 10.1186/s12970-021-00472-y.

    PubMed 34965876
  • Dominguez R, Mate-Munoz JL, Cuenca E, Garcia-Fernandez P, Mata-Ordonez F, Lozano-Estevan MC, Veiga-Herreros P, da Silva SF, Garnacho-Castano MV. Effects of beetroot juice supplementation on intermittent high-intensity exercise efforts. J Int Soc Sports Nutr. 2018 Jan 5;15:2. doi: 10.1186/s12970-017-0204-9. eCollection 2018.

    PubMed 29311764
  • Delleli S, Ouergui I, Messaoudi H, Trabelsi K, Glenn JM, Ammar A, Chtourou H. Does Beetroot Supplementation Improve Performance in Combat Sports Athletes? A Systematic Review of Randomized Controlled Trials. Nutrients. 2023 Jan 12;15(2):398. doi: 10.3390/nu15020398.

    PubMed 36678270

Questions about this trial

What is this clinical trial studying?

A study testing 40 meters sprint, Fitness test by bronco test and Maximal strength in people with Athletic Performance, run by Universidad Nacional de Colombia at 1 site in Colombia.

The full registered description is in the About this study section and the official record.

Who may be eligible to take part?

The sponsor lists 3 inclusion and 4 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.

Is this clinical trial currently recruiting?

The record says: finished, as of the sponsor's last update on 12 Dec 2025. Status can change between updates, so confirm with the study contact before making plans.

Where is the study taking place?

The record lists 1 site in Colombia. Whether a site is taking part right now should be confirmed with that site. See every site.

What are the possible benefits and risks?

They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.

Are results available for this study?

Yes. Results are posted in the registry (first posted 12 Dec 2025). A summary is in the For doctors view, and the full results are on ClinicalTrials.gov.

How can a doctor refer a patient or contact the research team?

The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.

What does the primary outcome measure?

Results Obtained in the Physical Tests to Evaluate Impact on Acceleration (time frame: First and second physical tests weeks). The study lists 8 primary outcomes in all. It is what the study was designed to measure, not a finding.

Where can I read the original study information?

On the ClinicalTrials.gov record for NCT07106489, which also lists 13 linked publications.

Does a listing mean the treatment is approved or proven to work?

No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.

How often is this information updated?

AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 12 Dec 2025.

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