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Healthy Subjects (HS)NCT07114146

Physiological and Mood Changes After Walking in Green Spaces. A Comparison of Environments.

A study testing WALK THROUGH ENVIRONMENT A, WALK THROUGH ENVIRONMENT B and WALK THROUGH ENVIRONMENT C in people with Healthy Subjects (HS) and Forest Therapy, run by University of Vigo at 1 site in Spain.

Completed

Finished. The study has ended and is not enrolling. Results may be posted on the official record.

Official record
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Trial summary

Natural environments, and more specifically forest environments, have shown in the last decade a growing interest as a protection against the frenetic pace of life of a large part of the urban population in developed countries, where symptoms related to stress and anxiety are associated with an increasingly frequent… Read more about this study

Who is it for?
Ages 50 years and olderHealthy volunteers can take part
What is being tested?
WALK THROUGH ENVIRONMENT A, WALK THROUGH ENVIRONMENT B and WALK THROUGH ENVIRONMENT C
Could I receive a placebo?
No, everyone receives an active treatment
How many people
16 participants
Where
1 siteSpain
Study dates
27 Jul 2025to 30 Oct 2025
Phase
Interventional
Are there results?
None posted in the registry

Key requirements

Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.

  • Ages 50 years and older
  • All sexes
  • Healthy volunteers accepted
  • Adult, Older adult

Who may be able to join

  • Those who have the ability and physical strength to walk 3 km in a calm manner without presenting or consciously suffering any type of adverse symptom.

Who cannot join

  • Pregnancy
  • History of severe physical or mental illness
  • Drug addiction (including narcotic drugs and non-narcotics) habitual.
  • High risk of fall
  • Severe outdoor allergies

Trial locations and contacts

1 site in 1 country.

Spain1 site
  • Universidad de Vigo. Campus Pontevedra

    Pontevedra, Pontevedra, 36005

Other trials in Spain

About this study

Official title: Changes in Heart Rate Variability, Heart Rate, Blood Pressure, and Mood After Walking in Green Spaces. A Comparison of Environments With Healthy Adults.

Natural environments, and more specifically forest environments, have shown in the last decade a growing interest as a protection against the frenetic pace of life of a large part of the urban population in developed countries, where symptoms related to stress and anxiety are associated with an increasingly frequent state of sub-health.

The investigators propose to perform a randomized crossover study to analyze the effects of a walk in the forest on physiological and psychological markers related to autonomic nervous system activity in healthy adult. In addition, the investigators intend to observe whether the characteristics and perceptions of the forest environment could affect the results obtained in these markers using a scale created for this purpose.

Read more

Participants will be healthy adults with no history of serious physical or psychological illnesses, who will be exposed to three forest environments.

Physiological markers will be measured, including heart rate variability, which will be measured with a heart rate sensor that will monitor data throughout the exposure period, and heart rate and systolic and diastolic blood pressure, which will be measured with an automated device before and after each exposure. Before and after the walk through each of the forest environments, mood will be measured using the POMS scale, and our scale will be used to measure the perceived environmental quality after exposure to each environment.

In addition to the walk through the three forest environments, a control environment on an indoor treadmill will be used as a comparator, where distance, slope and walking speed will be adjusted to replicate the intensity and physical demand of the three outdoor routes.

The four interventions will be organized to create three different exposure sequences. Each exposure sequence will be randomly assigned to a different group. In turn, each participant will be randomly assigned to one of the three groups, so that all participants will undergo all four interventions in a repeated measures design.

Our hypothesis supports the idea that exposure to forest environments causes changes in physiological parameters and mood associated with positive regulation for health and for the autonomic nervous system in healthy adults compared to a neutral non-forest environment. As a second hypothesis, environmental characteristics as well as the perceived quality of the forest environment would be related to the magnitude of such changes associated with autonomic nervous system regulation and mood in healthy adults.

Being tested
WALK THROUGH ENVIRONMENT A, WALK THROUGH ENVIRONMENT B, WALK THROUGH ENVIRONMENT C, WALK THROUGH ENVIRONMENT D
Keywords
Forest therapy, Autonomic nervous system, Mood, forest

What participants receive

What participants receive. In a randomised study each person is assigned to one group by chance.

  • WALK THROUGH ENVIRONMENT AOther

    A 3 km solo hike at a leisurely walking pace on a path that is easy to walk and has very little elevation gain. Located in Pontevedra Province.

  • WALK THROUGH ENVIRONMENT BOther

    A 3 km solo hike at a leisurely pace on an easy walking trail with very little elevation gain. Located in the province of Pontevedra.

  • WALK THROUGH ENVIRONMENT COther

    3 km solo hike at a leisurely pace on an easy trail with little elevation gain. Located in the province of Pontevedra.

  • WALK THROUGH ENVIRONMENT DOther

    Individual 3 km treadmill walk in a closed room facing a wall without pictures or windows at a leisurely walking pace with no gradient. This room will be located on the Pontevedra University Campus.

Study groups in NCT07114146
Study groupRoleWhat they receive
EXPOSURE IN SEQUENCE OF A-B-C-D ENVIRONMENTSExperimentalNot described
Receives: Other: WALK THROUGH ENVIRONMENT A; Other: WALK THROUGH ENVIRONMENT B; Other: WALK THROUGH ENVIRONMENT C; Other: WALK THROUGH ENVIRONMENT D
EXPOSURE IN SEQUENCE OF B-C-A-D ENVIRONMENTSExperimentalNot described
Receives: Other: WALK THROUGH ENVIRONMENT A; Other: WALK THROUGH ENVIRONMENT B; Other: WALK THROUGH ENVIRONMENT C; Other: WALK THROUGH ENVIRONMENT D
EXPOSURE IN SEQUENCE OF C-A-B-D ENVIRONMENTSExperimentalNot described
Receives: Other: WALK THROUGH ENVIRONMENT A; Other: WALK THROUGH ENVIRONMENT B; Other: WALK THROUGH ENVIRONMENT C; Other: WALK THROUGH ENVIRONMENT D

What is being measured

The primary outcome is the main question the study is designed to answer.

  1. CHANGES IN RR INTERVAL DURING FOREST EXPOSURE

    We will measure this variable continuously from 5 minutes before the start of each walk to 5 minutes after the end of each walk.

    The RR interval is a parameter related to Heart Rate Variability, its analysis is commonly used to evaluate the functioning of cardiac autonomic regulation.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places. From 5 minutes before the start of the 3 km walk until 5 minutes after the end of the walk.

  2. CHANGES IN Root Mean Square of Successive Difference Root of RR Interval (RMSSD) DURING FOREST EXPOSURE

    We will measure this variable continuously from 5 minutes before the start of each walk to 5 minutes after the end of each walk.

    The RMSSD is a parameter related to Heart Rate Variability, its analysis is commonly used to evaluate the functioning of cardiac autonomic regulation.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places. From 5 minutes before the start of the 3 km walk until 5 minutes after the end of the walk.

  3. CHANGES IN standard deviation of RR intervals (SDNN) DURING FOREST EXPOSURE

    We will measure this variable continuously from 5 minutes before the start of each walk to 5 minutes after the end of each walk.

    The SDNN is a parameter related to Heart Rate Variability, its analysis is commonly used to evaluate the functioning of cardiac autonomic regulation.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places. From 5 minutes before the start of the 3 km walk until 5 minutes after the end of the walk.

  4. CHANGES IN High frequency component (HF) DURING FOREST EXPOSURE

    We will measure this variable continuously from 5 minutes before the start of each walk to 5 minutes after the end of each walk.

    The HF component is a parameter related to Heart Rate Variability, its analysis is commonly used to evaluate the functioning of cardiac autonomic regulation.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places. From 5 minutes before the start of the 3 km walk until 5 minutes after the end of the walk.

  5. CHANGES IN Low frequency component (LF) DURING FOREST EXPOSURE

    We will measure this variable continuously from 5 minutes before the start of each walk to 5 minutes after the end of each walk.

    The LF component is a parameter related to Heart Rate Variability, its analysis is commonly used to evaluate the functioning of cardiac autonomic regulation.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places. From 5 minutes before the start of the 3 km walk until 5 minutes after the end of the walk.

  6. CHANGES IN Sympathovagal balance (LF/HF) DURING FOREST EXPOSURE

    We will measure this variable continuously from 5 minutes before the start of each walk to 5 minutes after the end of each walk.

    The sympathovagal balance is a parameter related to Heart Rate Variability, its analysis is commonly used to evaluate the functioning of cardiac autonomic regulation.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places. From 5 minutes before the start of the 3 km walk until 5 minutes after the end of the walk.

  7. CHANGES IN HEART RATE

    We will measure this variable punctually on four occasions each of the 4 days of intervention. This data will be taken with an Automated Blood Pressure Device placed on the left arm with participants resting in a seated position. Each measurement is taken three times. Participants rest for 5 minutes before the measurements.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places: Baseline (min 0), 5 minutes pre walk, 5 minutes post walk and after the period of time corresponding to the vehicle journey.

  8. CHANGES IN SYSTOLIC BLOOD PRESSURE

    We will measure this variable punctually on four occasions each of the 4 days of intervention. This data will be taken with an Automated Blood Pressure Device placed on the left arm with participants resting in a seated position. Each measurement is taken three times. Participants rest for 5 minutes before the measurements.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places: Baseline (min 0), 5 minutes pre walk, 5 minutes post walk and after the period of time corresponding to the vehicle journey.

  9. CHANGES IN DIASTOLIC BLOOD PRESSURE

    We will measure this variable punctually on four occasions each of the 4 days of intervention. This data will be taken with an Automated Blood Pressure Device placed on the left arm with participants resting in a seated position. Each measurement is taken three times. Participants rest for 5 minutes before the measurements.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places: Baseline (min 0), 5 minutes pre walk, 5 minutes post walk and after the period of time corresponding to the vehicle journey.

  10. CHANGES IN THE PROFILE OF MOOD STATES. SHORT SPANISH VERSION

    We will measure this variable punctually on two occasions each of the 4 days of intervention. The POMS is a questionnaire for the measurement of mood. POMS is a reliable and valid psychometric instrument that includes 30 adjectives in this Spanish short versions, rated on a scale of 0-4 (0 = none; 4 = very strong), which can be integrated into six factors, four of these factors are negative: Anger, Fatigue, Tension and Depressed State; and two are positive: Vigour and Friendship.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places: Baseline (min 0) and post walk immediately after finishing physiological measurements

  11. CHANGES IN TOTAL MOOD DISTURBANCE

    We will measure this variable punctually on two occasions each of the 4 days of intervention. Total Mood Disturbance TMD It is a score that measures a person's emotional distress, psychological distress or subjective well-being. It can be obtained from POMS results; it is calculated from the sum of all the values obtained for each factor, with the exception of the two positive factors, which are subtracted.

    Measured: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places: Baseline (min 0) and post walk immediately after finishing physiological measurements

  12. SCALE OF PERCEIVED ENVIRONMENTAL QUALITY OF THE FOREST ENVIRONMENT

    We will measure this variable once each of the 4 days of intervention. We will use the "Scale of perceived environmental quality of the forest environment", a 41-question recording method to determine the quality perceived by the respondent of a given forest environment.

    It is configured by means of a series of questions in which the person quantitatively rates certain characteristics of the tree stands according to his or her own criteria. 39 of the questions use a 5-point Likert scale in which each question is rated from 1 to 5, with 1 = Not at all, and 5 = Very much. Questions 40 and 41 are called criterion questions where on a scale of 1 to 10 the person responds according to his or her criteria 1 = very low/very little and 10 = outstanding.

    Measured: This measurement is performed on each of the three days when subjects take a walk in each of the three outdoor study environments: Post walk, immediately after completing the physiological measurements.

Timeline

From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.

  1. First posted11 Aug 2025
  2. Study start27 Jul 2025
  3. Primary completion22 Sep 2025
  4. Study completion30 Oct 2025

Resources

More details

For doctors and researchers: how the study is designed, who runs it, and its registry IDs.

Study design
Study type
Interventional
Phase
Phase N/A
Primary purpose
Treatment
Allocation
Randomised
Intervention model
Crossover
Masking
Single blind
Who is masked
Care provider
Enrolment
16 participants (actual)
IDs, dates and publications
ClinicalTrials.gov ID
NCT07114146
Sponsor's protocol ID
ETICA-ULE-087-2025
Status last verified
Apr 2026
First submitted
28 Jul 2025
Last updated
23 Apr 2026
Results posted
No
Data monitoring committee
No
FDA-regulated drug
No
FDA-regulated device
No
Individual data sharing
Undecided

Questions about this trial

What is this clinical trial studying?

A study testing WALK THROUGH ENVIRONMENT A, WALK THROUGH ENVIRONMENT B and WALK THROUGH ENVIRONMENT C in people with Healthy Subjects (HS) and Forest Therapy, run by University of Vigo at 1 site in Spain.

The full registered description is in the About this study section and the official record.

Who may be eligible to take part?

The sponsor lists 1 inclusion and 5 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.

Is this clinical trial currently recruiting?

The record says: finished, as of the sponsor's last update on 23 Apr 2026. Status can change between updates, so confirm with the study contact before making plans.

Where is the study taking place?

The record lists 1 site in Spain. Whether a site is taking part right now should be confirmed with that site. See every site.

What are the possible benefits and risks?

They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.

Are results available for this study?

The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.

How can a doctor refer a patient or contact the research team?

The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.

What does the primary outcome measure?

CHANGES IN RR INTERVAL DURING FOREST EXPOSURE (time frame: This measurement is carried out each of the four days in which the subjects take a walk in each of the four study places. From 5 minutes before the start of the 3 km walk until 5 minutes after the end of the walk.). The study lists 12 primary outcomes in all. It is what the study was designed to measure, not a finding.

Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?

No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.

How often is this information updated?

AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 23 Apr 2026.

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