Dietary SupplementMORISINGFEMALENCT07129863
Caffeine Mouth Rinsing at Different Doses in Female Team-Sport Athletes: A Randomized, Double-Blind, Placebo-Controlled Trial
A study testing Experimental: Low mouth rinse caffeine concentration (1%), Moderate caffeine concentration (2% caffeine solution weight/volume). and High caffeine concentration (3% caffeine solution weight/volume). in people with Dietary Supplement and Sports Performance, run by Universidad Pontificia Comillas at 1 site in Turkey (Türkiye).
Trial summary
Objective: To evaluate the effects of different caffeine mouth rinse concentrations (1%, 2%, and 3%) on Running Anaerobic Sprint Test (RAST) performance in female team-sport athletes. Main Questions: 1. Do caffeine mouth rinses improve RAST performance in female team-sport athletes? 2. Read more about this study
- Who is it for?
- Ages 18 to 35 years, women onlyHealthy volunteers can take part
- Conditions the study is for
- Dietary Supplement, Sports Performance
- What is being tested?
- Experimental: Low mouth rinse caffeine concentration (1%), Moderate caffeine concentration (2% caffeine solution weight/volume). and High caffeine concentration (3% caffeine solution weight/volume).
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 13 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 24 Sep 2023to 22 Dec 2024
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 35 years
- Women only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Female team sports athletes aged between 18 and 35 years.
- Currently engaged in team sports.
- Non-smoker.
Who cannot join
- Participants were excluded if they met any of the following conditions.
- Presence of a musculoskeletal injury within the six months prior to the study.
- Current smoker.
- Use of nutritional supplements or ergogenic aids that could influence sprint running performance (e.g., beta-alanine, creatine).
- Use of oral contraceptives.
- Known allergy to caffeine.
- Failure to meet the training requirement of at least six hours of team-based practice, a minimum of three days per week, over the past year.
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Eastern Mediterranean University
Mersin, SBF C 116
About this study
Official title: Caffeine Mouth Rinsing at Different Doses Does Not Enhance Anaerobic Sprint Performance in Female Team-Sport Athletes: A Randomized, Double-Blind, Placebo-Controlled Trial
Objective:
To evaluate the effects of different caffeine mouth rinse concentrations (1%, 2%, and 3%) on Running Anaerobic Sprint Test (RAST) performance in female team-sport athletes.
Main Questions:
- Do caffeine mouth rinses improve RAST performance in female team-sport athletes?
- Do different caffeine mouth rinse concentrations affect ratings of perceived exertion (RPE)?
Method:
Thirteen trained female football and handball players (23.0 ± 4.5 years) completed four randomized, double-blind, crossover conditions (1%, 2%, and 3% caffeine mouth rinses, and placebo). Each trial involved six 35 m sprints separated by 10 s of rest. RPE was recorded immediately after each session.
Read the detailed description
Objective:
To evaluate the acute effects of different caffeine mouth rinse concentrations (1%, 2%, and 3%) on anaerobic sprint performance and perceived exertion in trained female team-sport athletes. The study sought to determine whether this non-ingestive ergogenic strategy could enhance high-intensity running performance and reduce effort perception.
Main Questions:
- Do different caffeine mouth rinse concentrations improve Running Anaerobic Sprint Test (RAST) performance in trained female team-sport athletes?
- Do caffeine mouth rinses at varying concentrations influence ratings of perceived exertion (RPE) compared with placebo?
Is there an optimal caffeine concentration that balances potential performance benefits with reduced perceived exertion?
Method:
Thirteen trained female athletes (mean age 23.0 ± 4.5 years) competing in football and handball participated in a randomized, double-blind, placebo-controlled, crossover trial. Each athlete completed four experimental conditions: placebo, 1% caffeine mouth rinse, 2% caffeine mouth rinse, and 3% caffeine mouth rinse. In each session, participants performed a Running Anaerobic Sprint Test (RAST) consisting of six maximal 35 m sprints separated by 10 seconds of passive recovery. The RPE was assessed immediately after each test using a 1-10 scale.
- Conditions
- Dietary Supplement, Sports Performance
- Being tested
- Placebo: Mouth rinse with plain water, Experimental: Low mouth rinse caffeine concentration (1%), Moderate caffeine concentration (2% caffeine solution weight/volume)., High caffeine concentration (3% caffeine solution weight/volume).
- Keywords
- carbohydrate, nutrition, mouth rinse, placebo
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Placebo: Mouth rinse with plain waterDietary supplement
Placebo: Mouth rinse with plain water, containing no caffeine, serving as the control condition.
Experimental: Low mouth rinse caffeine concentration (1%)Dietary supplement
Arm Description: Low caffeine concentration: Mouth rinse containing 1% caffeine solution (based on weight/volume).
Moderate caffeine concentration (2% caffeine solution weight/volume).Dietary supplement
Moderate caffeine concentration: Mouth rinse containing 2% caffeine solution (weight/volume).
High caffeine concentration (3% caffeine solution weight/volume).Dietary supplement
High caffeine concentration: Mouth rinse containing 3% caffeine solution (weight/volume).
| Study group | Role | What they receive |
|---|---|---|
| Placebo (water) | Placebo comparator | Placebo: Mouth rinse with plain water, containing no caffeine, serving as the control condition. Receives: Dietary Supplement: Placebo: Mouth rinse with plain water |
| Low mouth rinse caffeine concentration | Experimental | Low caffeine concentration: Mouth rinse containing 1% caffeine solution (based on weight/volume). Receives: Dietary Supplement: Experimental: Low mouth rinse caffeine concentration (1%) |
| Moderate mouth rinse caffeine concentration | Experimental | Moderate caffeine concentration: Mouth rinse containing 2% caffeine solution (based on weight/volume). Receives: Dietary Supplement: Moderate caffeine concentration (2% caffeine solution weight/volume). |
| High mouth rinse caffeine concentration | Experimental | Moderate caffeine concentration: Mouth rinse containing 3% caffeine solution (based on weight/volume). Receives: Dietary Supplement: High caffeine concentration (3% caffeine solution weight/volume). |
What is being measured
The primary outcome is the main question the study is designed to answer.
Running-based anaerobic sprint test (RAST protocol)
The RAST was comprised of six 35 m sprints at maximum effort, each of which was followed by a 10 s passive rest period (including deceleration phase). Participants rinsed the solution in the final 5 s of the rest period accompanied by the counting of the tester, and then sprint back to the first timing gate with "GO" command. This was repeated until six sprints had been accomplished. Participants began to sprint running for a maximum of one step before the timing gate and were instructed not to decelerate before passing through the timing gate. Using a system of photocells (Newtest Powertimer 300-series, Newtest Oy, Tyrnävä, Finland), which was placed at the beginning and the end of the 35 m, the duration of each 35 m sprint running time was recorded. Using time of each sprint attempt, 1-) fastest sprint time, 2-) mean sprint time, and 3-) fatigue index were recorded
Measured: 2-week
Secondary outcomes (1)
Rating of perceived exertion (RPE)
Perceived exertion during each trial was assessed immediately after the Running Anaerobic Sprint Test (RAST) using a 1-10 numerical scale points, where:
1 = Very, very light effort (minimal exertion, easy to continue)
2-3 = Light effort (comfortable, mild strain)
4-5 = Moderate effort (noticeable breathing and muscle activation)
6-7 = Hard effort (challenging but sustainable for a short time)
8-9 = Very hard effort (significant discomfort, near maximal effort)
10 = Maximal effort (unable to continue beyond current level, exhaustion)
This scale provided a subjective measure of the athletes' internal load, complementing the objective performance metrics from the sprint test.
Measured: 2-weeks
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted19 Aug 2025
- Study start24 Sep 2023
- Primary completion1 Dec 2024
- Study completion22 Dec 2024
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Dietary SupplementStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Triple blind
- Who is masked
- Participant, Care provider, Investigator
- Enrolment
- 13 participants (actual)
Sponsor and investigators
- Lead sponsor
- Universidad Pontificia Comillas
- Sponsor type
- University, hospital or other
- Collaborators
- Eastern Mediterranean University, Northumbria University, Universidad Francisco de Vitoria, Ankara University
- Responsible party
- Sponsor
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07129863
- Sponsor's protocol ID
- UPC-Comillas
- Acronym
- MORISINGFEMALE
- Status last verified
- Aug 2025
- First submitted
- 11 Aug 2025
- Last updated
- 19 Aug 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No: IPD will be shared if previously is required
Study at a glance
From the registry record, last updated by the sponsor on 19 Aug 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Caffeine Mouth Rinsing at Different Doses Does Not Enhance Anaerobic Sprint Performance in Female Team-Sport Athletes: A Randomized, Double-Blind, Placebo-Controlled Trial
- ClinicalTrials.gov ID
- NCT07129863
- Acronym / sponsor ID
- MORISINGFEMALE / UPC-Comillas
- Conditions
- Dietary Supplement, Sports Performance
- Keywords
- carbohydrate, nutrition, mouth rinse, placebo
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 13 participants (actual)
- Lead sponsor
- Universidad Pontificia ComillasUniversity, hospital or other
- Collaborators
- Eastern Mediterranean University, Northumbria University, Universidad Francisco de Vitoria, Ankara University
- Study start
- 24 Sep 2023 (actual)
- Primary completion
- 1 Dec 2024 (actual)
- Study completion
- 22 Dec 2024 (actual)
- First posted
- 19 Aug 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Objective:
To evaluate the effects of different caffeine mouth rinse concentrations (1%, 2%, and 3%) on Running Anaerobic Sprint Test (RAST) performance in female team-sport athletes.
Main Questions:
- Do caffeine mouth rinses improve RAST performance in female team-sport athletes?
- Do different caffeine mouth rinse concentrations affect ratings of perceived exertion (RPE)?
Method:
Thirteen trained female football and handball players (23.0 ± 4.5 years) completed four randomized, double-blind, crossover conditions (1%, 2%, and 3% caffeine mouth rinses, and placebo). Each trial involved six 35 m sprints separated by 10 s of rest. RPE was recorded immediately after each session.
Detailed description
Objective:
To evaluate the acute effects of different caffeine mouth rinse concentrations (1%, 2%, and 3%) on anaerobic sprint performance and perceived exertion in trained female team-sport athletes. The study sought to determine whether this non-ingestive ergogenic strategy could enhance high-intensity running performance and reduce effort perception.
Main Questions:
- Do different caffeine mouth rinse concentrations improve Running Anaerobic Sprint Test (RAST) performance in trained female team-sport athletes?
- Do caffeine mouth rinses at varying concentrations influence ratings of perceived exertion (RPE) compared with placebo?
Is there an optimal caffeine concentration that balances potential performance benefits with reduced perceived exertion?
Method:
Thirteen trained female athletes (mean age 23.0 ± 4.5 years) competing in football and handball participated in a randomized, double-blind, placebo-controlled, crossover trial. Each athlete completed four experimental conditions: placebo, 1% caffeine mouth rinse, 2% caffeine mouth rinse, and 3% caffeine mouth rinse. In each session, participants performed a Running Anaerobic Sprint Test (RAST) consisting of six maximal 35 m sprints separated by 10 seconds of passive recovery. The RPE was assessed immediately after each test using a 1-10 scale.
Study design
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Triple blind (participant, care provider, investigator)
- Number of arms
- 4
| Arm | Type | Receives |
|---|---|---|
| Placebo (water) | Placebo comparator | Dietary Supplement: Placebo: Mouth rinse with plain waterPlacebo: Mouth rinse with plain water, containing no caffeine, serving as the control condition. |
| Low mouth rinse caffeine concentration | Experimental | Dietary Supplement: Experimental: Low mouth rinse caffeine concentration (1%)Low caffeine concentration: Mouth rinse containing 1% caffeine solution (based on weight/volume). |
| Moderate mouth rinse caffeine concentration | Experimental | Dietary Supplement: Moderate caffeine concentration (2% caffeine solution weight/volume).Moderate caffeine concentration: Mouth rinse containing 2% caffeine solution (based on weight/volume). |
| High mouth rinse caffeine concentration | Experimental | Dietary Supplement: High caffeine concentration (3% caffeine solution weight/volume).Moderate caffeine concentration: Mouth rinse containing 3% caffeine solution (based on weight/volume). |
Who may qualify
- Ages
- 18 to 35 years
- Sex
- Female only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (3 listed)
- Female team sports athletes aged between 18 and 35 years.
- Currently engaged in team sports.
- Non-smoker.
Key exclusion criteria (7 listed)
- Participants were excluded if they met any of the following conditions.
- Presence of a musculoskeletal injury within the six months prior to the study.
- Current smoker.
- Use of nutritional supplements or ergogenic aids that could influence sprint running performance (e.g., beta-alanine, creatine).
- Use of oral contraceptives.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Placebo: Mouth rinse with plain waterPlacebo: Mouth rinse with plain water, containing no caffeine, serving as the control condition. | Dietary supplement | Placebo (water) |
| Experimental: Low mouth rinse caffeine concentration (1%)Arm Description: Low caffeine concentration: Mouth rinse containing 1% caffeine solution (based on weight/volume). | Dietary supplement | Low mouth rinse caffeine concentration |
| Moderate caffeine concentration (2% caffeine solution weight/volume).Moderate caffeine concentration: Mouth rinse containing 2% caffeine solution (weight/volume). | Dietary supplement | Moderate mouth rinse caffeine concentration |
| High caffeine concentration (3% caffeine solution weight/volume).High caffeine concentration: Mouth rinse containing 3% caffeine solution (weight/volume). | Dietary supplement | High mouth rinse caffeine concentration |
Primary outcome
Running-based anaerobic sprint test (RAST protocol)
Time frame: 2-week
The RAST was comprised of six 35 m sprints at maximum effort, each of which was followed by a 10 s passive rest period (including deceleration phase). Participants rinsed the solution in the final 5 s of the rest period accompanied by the counting of the tester, and then sprint back to the first timing gate with "GO" command. This was repeated until six sprints had been accomplished. Participants began to sprint running for a maximum of one step before the timing gate and were instructed not to decelerate before passing through the timing gate. Using a system of photocells (Newtest Powertimer 300-series, Newtest Oy, Tyrnävä, Finland), which was placed at the beginning and the end of the 35 m, the duration of each 35 m sprint running time was recorded. Using time of each sprint attempt, 1-) fastest sprint time, 2-) mean sprint time, and 3-) fatigue index were recorded
1 secondary outcome
Rating of perceived exertion (RPE)
Time frame: 2-weeks
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 19 Aug 2025.
- Individual participant dataSharing planned: No. IPD will be shared if previously is required
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Experimental: Low mouth rinse caffeine concentration (1%), Moderate caffeine concentration (2% caffeine solution weight/volume). and High caffeine concentration (3% caffeine solution weight/volume). in people with Dietary Supplement and Sports Performance, run by Universidad Pontificia Comillas at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 3 inclusion and 7 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 19 Aug 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Running-based anaerobic sprint test (RAST protocol) (time frame: 2-week). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 19 Aug 2025.