Protein MetabolismYPQNCT07148908
Animal and Microbial-based Dietary Protein Efficiency in Adults
A study testing Yeast Protein Supplement, Whey Protein Isolate Supplement and Collagen Hydrolysate Supplement in people with Protein Metabolism, run by Daniel Moore at 1 site in Canada.
Trial summary
Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. Read more about this study
- Who is it for?
- Ages 18 to 45 yearsHealthy volunteers can take part
- Conditions the study is for
- Protein Metabolism
- What is being tested?
- Yeast Protein Supplement, Whey Protein Isolate Supplement and Collagen Hydrolysate Supplement
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 13 participants
- Where
- 1 siteCanada
- Study dates
- 1 Jul 2025to 31 Dec 2025
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 45 years
- All sexes
- Healthy volunteers accepted
- Adult
Who may be able to join
- Healthy, male and female, recreationally-active participants.
- Healthy will be defined as screened by the PAR-Q+ (Appendix B) and The Physical Activity Readiness Questionnaire (Appendix C).
- Participants will be aged 18-45 years old.
- Participants are willing to abide by the compliance rules of this study.
- Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
- Use of monophasic combined oral contraceptives (COC) containing 21 active pills (e.g., Yaz, Marvelon, Cyclen, etc.; the brand of COC will be recorded) (female participants).
Who cannot join
- Inability to adhere to any of the compliance rules judged by principal investigator (e.g. dairy protein or yeast allergy).
- Self-reported regular tobacco use.
- Self-reported illicit drug use (e.g., growth hormone, testosterone, etc.).
- Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
- Use of multiphasic COCs due to the difficulty of controlling for hormonal fluctuations in such formulations (female participants).
- Use of COCs containing >21 active pills per cycle due to potential differences in their effect on metabolism (female participants).
- Use of other forms of hormonal contraception (e.g., progestin-only pill, hormonal IUD, intravaginal ring) (female participants).
- Not currently or previously (in the past 6 months) on a vegan diet
Trial locations and contacts
1 site in 1 country. Contact the study team or a site near you to ask about taking part.
Canada1 site, 1 recruiting
- Recruiting
Kinesiology & Physical Education
Toronto, Ontario, M5S 2C9
Sabrina T. Barsky, Ph.D., 647-540-0115, sabrina.barsky@utoronto.ca
About this study
Official title: The Effects of Animal- and Microbial-based Protein Sources on Whole-body Protein Metabolism in Healthy Adults
Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. The overall objective of this investigation is to examine the impact of different animal and microbial-based protein sources to support whole body protein synthesis in adults. To do this, investigators will employ a 'breath test' method developed in our laboratory as well as urine sampling. The results of this study will allow us to better understand the impact of dietary protein quality for maintaining health and body protein mass in adults.
- Conditions
- Protein Metabolism
- Being tested
- Yeast Protein Supplement, Whey Protein Isolate Supplement, Collagen Hydrolysate Supplement
- Keywords
- Protein quality, protein metabolism, stable isotopes, protein oxidation, indicator amino acid oxidation, protein synthesis
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Yeast Protein SupplementDietary supplement
Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks
Whey Protein Isolate SupplementDietary supplement
Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks
Collagen Hydrolysate SupplementDietary supplement
Each protein will be consumed at 0.9g/kg/d in n=8 hourly drinks
| Study group | Role | What they receive |
|---|---|---|
| Yeast Protein Supplement | Experimental | Participants will be consuming test beverage composed of yeast protein and their whole-body protein metabolism will be determined over the subsequent 8 hours. Receives: Dietary Supplement: Yeast Protein Supplement |
| Whey Protein Isolate Supplement | Active comparator | Participants will be consuming test beverage composed of whey protein isolate and their whole-body protein metabolism will be determined over the subsequent 8 hours. Receives: Dietary Supplement: Whey Protein Isolate Supplement |
| Collagen Hydrolysate Supplement | Active comparator | Participants will be consuming test beverage composed of collagen protein and their whole-body protein metabolism will be determined over the subsequent 8 hours. Receives: Dietary Supplement: Collagen Hydrolysate Supplement |
What is being measured
The primary outcome is the main question the study is designed to answer.
1. Phenylalanine excretion (umol/kg/h)
Whole body phenylalanine excretion determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2).
Measured: 8 hours
Phenylalanine oxidation (umol/kg/h)
Whole-body phenylalanine oxidation determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment determined from urine enrichment over the 8 hour measurement period.
Measured: 8 hours
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted29 Aug 2025
- Study start1 Jul 2025
- Primary completion31 Oct 2025 (estimated)
- Study completion31 Dec 2025 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Protein MetabolismStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Crossover
- Model description
- Single-blind randomized, counterbalanced design Thirteen healthy young (18-45 years of age, at least 6 females and 6 males) will be subjected to a non-invasive 13CO2 breath-test based on the indicator amino acid oxidation method and urine collections following hourly ingestion of protein test beverages (whey, yeast, and collagen randomized by trials).
- Masking
- Single blind
- Who is masked
- Participant
- Enrolment
- 13 participants (estimated)
Sponsor and investigators
- Lead sponsor
- Daniel Moore
- Sponsor type
- University, hospital or other
- Collaborators
- Lesaffre International
- Responsible party
- Sponsor-investigator, Daniel Moore, University of Toronto
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07148908
- Sponsor's protocol ID
- REB00048239
- Acronym
- YPQ
- Status last verified
- Aug 2025
- First submitted
- 22 Aug 2025
- Last updated
- 29 Aug 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Study at a glance
From the registry record, last updated by the sponsor on 29 Aug 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Effects of Animal- and Microbial-based Protein Sources on Whole-body Protein Metabolism in Healthy Adults
- ClinicalTrials.gov ID
- NCT07148908
- Acronym / sponsor ID
- YPQ / REB00048239
- Conditions
- Protein Metabolism
- Keywords
- Protein quality, protein metabolism, stable isotopes, protein oxidation, indicator amino acid oxidation, protein synthesis
- Study type
- Interventional
- Recruitment status
- Recruiting
- Enrolment
- 13 participants (estimated)
- Lead sponsor
- Daniel MooreUniversity, hospital or other
- Collaborators
- Lesaffre International
- Study start
- 1 Jul 2025 (actual)
- Primary completion
- 31 Oct 2025 (estimated)
- Study completion
- 31 Dec 2025 (estimated)
- First posted
- 29 Aug 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Given the relatively high carbon footprint and sustainability of animal-based proteins, there is a growing interest in determining the nutritional quality non-animal-based protein sources. The overall objective of this investigation is to examine the impact of different animal and microbial-based protein sources to support whole body protein synthesis in adults. To do this, investigators will employ a 'breath test' method developed in our laboratory as well as urine sampling. The results of this study will allow us to better understand the impact of dietary protein quality for maintaining health and body protein mass in adults.
Study design
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Single blind (participant)
- Number of arms
- 3
- Model description
- Single-blind randomized, counterbalanced design Thirteen healthy young (18-45 years of age, at least 6 females and 6 males) will be subjected to a non-invasive 13CO2 breath-test based on the indicator amino acid oxidation method and urine collections following hourly ingestion of protein test beverages (whey, yeast, and collagen randomized by trials).
| Arm | Type | Receives |
|---|---|---|
| Yeast Protein Supplement | Experimental | Dietary Supplement: Yeast Protein SupplementParticipants will be consuming test beverage composed of yeast protein and their whole-body protein metabolism will be determined over the subsequent 8 hours. |
| Whey Protein Isolate Supplement | Active comparator | Dietary Supplement: Whey Protein Isolate SupplementParticipants will be consuming test beverage composed of whey protein isolate and their whole-body protein metabolism will be determined over the subsequent 8 hours. |
| Collagen Hydrolysate Supplement | Active comparator | Dietary Supplement: Collagen Hydrolysate SupplementParticipants will be consuming test beverage composed of collagen protein and their whole-body protein metabolism will be determined over the subsequent 8 hours. |
Who may qualify
- Ages
- 18 to 45 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (6 listed)
- Healthy, male and female, recreationally-active participants.
- Healthy will be defined as screened by the PAR-Q+ (Appendix B) and The Physical Activity Readiness Questionnaire (Appendix C).
- Participants will be aged 18-45 years old.
- Participants are willing to abide by the compliance rules of this study.
- Self-reported regular menstrual cycle (25-35d) within the last 3 months (female participants).
Key exclusion criteria (8 listed)
- Inability to adhere to any of the compliance rules judged by principal investigator (e.g. dairy protein or yeast allergy).
- Self-reported regular tobacco use.
- Self-reported illicit drug use (e.g., growth hormone, testosterone, etc.).
- Individuals who have participated in studies within the past year involving any of the stable isotopes in the study.
- Use of multiphasic COCs due to the difficulty of controlling for hormonal fluctuations in such formulations (female participants).
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Yeast Protein SupplementEach protein will be consumed at 0.9g/kg/d in n=8 hourly drinks | Dietary supplement | Yeast Protein Supplement |
| Whey Protein Isolate SupplementEach protein will be consumed at 0.9g/kg/d in n=8 hourly drinks | Dietary supplement | Whey Protein Isolate Supplement |
| Collagen Hydrolysate SupplementEach protein will be consumed at 0.9g/kg/d in n=8 hourly drinks | Dietary supplement | Collagen Hydrolysate Supplement |
Primary outcome
1. Phenylalanine excretion (umol/kg/h)
Time frame: 8 hours
Whole body phenylalanine excretion determined from the product of breath 13CO2 enrichment and carbon dioxide production rate (VCO2).
Phenylalanine oxidation (umol/kg/h)
Time frame: 8 hours
Whole-body phenylalanine oxidation determined from the correction of phenylalanine excretion by the phenylalanine precursor enrichment determined from urine enrichment over the 8 hour measurement period.
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 29 Aug 2025.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Yeast Protein Supplement, Whey Protein Isolate Supplement and Collagen Hydrolysate Supplement in people with Protein Metabolism, run by Daniel Moore at 1 site in Canada.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 6 inclusion and 8 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: looking for participants now, as of the sponsor's last update on 29 Aug 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Canada. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
Contact Daniel R. Moore, Ph.D. (416-946-4088, drmoore@utoronto.ca), or a site near the patient. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
1. Phenylalanine excretion (umol/kg/h) (time frame: 8 hours). The study lists 2 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 29 Aug 2025.