Muscle DamageNCT07224867
The Effects of Liquid IV's Multi-Ingredient Recovery Supplement on Muscle Recovery Following Exercise
A study testing proprietary post workout recovery supplement in people with Muscle Damage and Strength Recovery, run by Applied Science & Performance Institute at 1 site in United States.
Trial summary
The purpose of this study is to evaluate the efficacy of a multi-ingredient recovery supplement . The research will focus on strength recovery and performance maintenance assessing reductions in soreness and inflammation, as well as muscle repair and damage mitigation. Read more about this study
- Who is it for?
- Ages 18 to 55 yearsHealthy volunteers can take part
- Conditions the study is for
- Muscle Damage, Strength Recovery, Perceived Recovery
- What is being tested?
- proprietary post workout recovery supplement
- Type of treatment
- Injection or infusion
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 0 participants
- Where
- 1 siteUnited States
- Study dates
- 1 Oct 2026to 31 Jan 2027
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 55 years
- All sexes
- Healthy volunteers accepted
- Adult
Who may be able to join
- Healthy males or females aged 18-55 years old.
- Must be recreationally trained or trained/developmental as previously defined (McKay et al., 2021).
- Able to read and write in English.
- Cessation of current use of tart cherry extract, protein powders, essential amino acids/BCAAs, calcium fructoborate, boron, or anabolic agents for a minimum of 1 week prior to, and throughout the duration of the study.
- Willing to maintain regular supplement routine otherwise during the trial, and will not introduce or discontinue a supplement, other than what was previously mentioned.
- Will not participate in any strenuous exercise for a minimum of 1-week prior to exercise performance assessments, as well as during the days of exercise testing. Strenuous exercise as defined for this study includes ½ and full marathons (or more), heavy (i.e.,>80% 1RM) lifting, and any high intensity, physical activity that could make your muscles sore.
- During the study, you agree not to take any NSAIDs or other anti-inflammatory medications.
Who cannot join
- Sedentary
- Highly trained/national level or professional athlete
- Known diagnosis of any cardiovascular, metabolic, musculoskeletal, endocrine, or renal disease/disorder.
- Recent musculoskeletal injury (<3-months)
- Recent orthopedic surgery (<12-months)
- History or current malignancy.
Show all 17Show fewer
- Current respiratory infection (i.e., cold) or high temperature (fever) within the past 4-weeks.
- Previous gastrointestinal surgery within the past 12 months.
- Regular smoker.
- Regular drinker (>14 drinks per week).
- Those regularly using anti-inflammatory drugs within the last 4-weeks.
- Current use of prescription medications that may influence adaptation to exercise (hormone therapies, peptides, etc.).
- Allergies to study-related materials.
- Any condition that impairs their ability to complete the required exercises.
- Female subjects must not be pregnant or lactating.
- Are unable to provide informed consent or are non-English speakers.
- Presently using GLP-1 medications.
Trial locations and contacts
1 site in 1 country.
United States1 site
Applied Science & Performance Institute
Tampa, Florida, 33634
About this study
Official title: The Effects of Liquid IV's Multi-Ingredient Recovery Supplement, on Muscle Recovery Following Exercise: A Randomized Crossover Study
The purpose of this study is to evaluate the efficacy of a multi-ingredient recovery supplement . The research will focus on strength recovery and performance maintenance assessing reductions in soreness and inflammation, as well as muscle repair and damage mitigation. By investigating these key recovery metrics, this study aims to investigate the efficacy of this supplement as a solution for active individuals seeking to optimize post-exercise recovery.
- Conditions
- Muscle Damage, Strength Recovery, Perceived Recovery, Perceived Muscle Soreness, Physical Performance, Muscle Recovery
- Being tested
- proprietary post workout recovery supplement, Control placebo
- Keywords
- muscle damage, muscle recovery, strength recovery, physical performance, perceived recovery, perceived muscle soreness
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
proprietary post workout recovery supplementDietary supplement
proprietary post workout recovery supplement
Control placeboDietary supplement
inactive/inert placebo mix
| Study group | Role | What they receive |
|---|---|---|
| proprietary post workout recovery supplement | Experimental | Liquid I.V. post workout recovery powder mixture Receives: Dietary Supplement: proprietary post workout recovery supplement |
| Control Treatment | Placebo comparator | inactive/inert placebo mixture Receives: Dietary Supplement: Control placebo |
What is being measured
The primary outcome is the main question the study is designed to answer.
Lower Body Power and Force Generation (Maximal Countermovement Jump (CMJ))
The force plate platform consists of two force plates (left \& right) containing four force sensors which measure ground reaction force. The sensors are connected to a laptop and analyzed using Mechanography GRFP Research Edition® software (version 4.2-b05.53-RES).
Subjects will perform three maximal vertical CMJs on a calibrated force plate. Subjects will be instructed to stand upright on the center of the force plate with feet shoulder-width apart and hands placed on the hips. Subjects will perform a maximal effort vertical jump with proper form and instructed to land with soft knees and remain still after each jump for data capture. A 30sec rest period will be provided between each trial. Data will be used to assess maximum force output (Fmax), rate of force development (RFD), jump height (inches), and maximum power (Pmax). Force curves will be reviewed for each trial, and results averaged. This will quantify full-body mechanical power under standardized, reproducible conditions.
Measured: Taken at baseline testing, 24 hour, 48 hour, and 72 hour.
Lower-Body Strength (Biodex Dynamometry)
Participants will complete isometric and isokinetic strength assessments, unilaterally on the dominant leg, of knee extension (ext) and flexion (flx) using a Biodex System 4 Pro dynamometer (Biodex Medical Systems, Shirley, NY). Proper positioning and stabilization will be ensured per manufacturer guidelines.
Isometric testing - knee joint will be fixed @60° of flexion. Participants will perform 3 maximal effort ext/flx contractions, each lasting 5sec, with 5sec of rest between efforts. Highest recorded torque value across the 3 trials will be analyzed.
Subjects will then complete isokinetic testing consisting of 5 maximal concentric knee ext and flx at an angular velocity of 60°/s. Full range of motion will be established prior to testing. Peak torque and average torque across all 5 repetitions will be recorded for analysis.
All torque values will be gravity-corrected by the system software, and same technician will conduct all tests for consistency.
Measured: Tested at baseline, 24 hour, 48 hour, and 72 hour.
Perceived Recovery Status (PRS) Likert Scale
The PRS is a composite scale directly proportional to recovery (Laurent, et al. 2011). Hence, the purpose of the scale will be to assess the participant's recovery from the previous exercise session. Subjects will be informed that having no soreness and a high feeling of recovery is a 10, while extreme soreness and low feelings of recovery, leading to limited ability to function, is defined by a range of 0-3, adequate recovery, with some ability to function, is defined by a range of 4-7, and good recovery, with the ability to function normally, is defined by a range of 8-10.
Measured: Taken at baseline testing, 24 hours, 48 hours, and 72 hours timepoints.
Perceived Body Soreness Likert Scale
A 7-point likert scale will be used for lower body muscle soreness, after performing 5 air squats. The Likert scale is a validated instrument designed to assess self reported muscle soreness, measured across a 7-point scale. The scale ranges from 0 (no soreness), to 6 (unbearable soreness), and has good validity and reliability (Impellizzeri, F., et al., 2007).
Measured: Taken at baseline testing, 24 hour, 48 hour, and 72 hour timepoints.
Numeric Pain Rating Scale (NPRS)
The NPRS is a subjective 11-point numeric pain rating scale, with 0 representing "no pain" and 10 representing "unbearable pain." The Likert scale is a validated instrument designed to assess self reported pain. For this study, the subjects will be asked to assign a number to their average lower extremity joint pain (hip and knee joint pain) (Hawker et al., 2011) at the designated time points.
Measured: NPRS will be taken at baseline, 24 hour, 48 hour and 72 hour time points
Secondary outcomes (1)
Body Hydration Status (InBody)
Body hydration status will be assessed using multi-frequency bioelectrical impedance analysis (BIA) (InBody 770, InBody Co., Ltd., Seoul, South Korea). The InBody device uses direct segmental multi-frequency impedance (5 kHz to 1 MHz) to estimate water distribution across body compartments. Measurements will include total body water (TBW), extracellular water (ECW), and intracellular water (ICW) to evaluate fluid compartment status. BIA assessments will be conducted under standardized conditions with participants barefoot, standing upright with arms slightly abducted, and heels positioned on the footplate electrodes. Light, non-conductive clothing will be required, and all metal (e.g., jewelry, watches) are removed before testing. To minimize fluid variability, participants will avoid food, drink, and caffeine for at least 8 hours prior to testing. Measurements will be collected pre-supplementation and post-exercise/post supplement as part of the hydration analysis at baseline only.
Measured: taken during baseline testing only - before exercise and again at ~10 minutes post treatment
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted5 Nov 2025
- Study start1 Oct 2026 (estimated)
- Primary completion1 Dec 2026 (estimated)
- Study completion31 Jan 2027 (estimated)
Why it stopped: sponsor decided not to move forward with study
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Publications (6)Papers the sponsor has linked to this study.
- Other trials for Muscle DamageStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Crossover
- Model description
- randomized, double-blind, crossover study
- Masking
- Double blind
- Who is masked
- Participant, Investigator
- Enrolment
- 0 participants (actual)
Sponsor and investigators
- Lead sponsor
- Applied Science & Performance Institute
- Sponsor type
- Industry (pharma, biotech, devices)
- Responsible party
- Sponsor
- Investigators
- Eric Sikorski, PhD, Principal investigator, Applied Science and Performance Institute
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07224867
- Sponsor's protocol ID
- Pro00089373
- Status last verified
- Jul 2026
- First submitted
- 3 Nov 2025
- Last updated
- 8 Jul 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Publications
Skurvydas A, Brazaitis M, Venckunas T, Kamandulis S. Predictive value of strength loss as an indicator of muscle damage across multiple drop jumps. Appl Physiol Nutr Metab. 2011 Jun;36(3):353-60. doi: 10.1139/h11-023. Epub 2011 May 16.
PubMed 21574783Shimomura Y, Yamamoto Y, Bajotto G, Sato J, Murakami T, Shimomura N, Kobayashi H, Mawatari K. Nutraceutical effects of branched-chain amino acids on skeletal muscle. J Nutr. 2006 Feb;136(2):529S-532S. doi: 10.1093/jn/136.2.529S.
PubMed 16424141Connolly DA, McHugh MP, Padilla-Zakour OI, Carlson L, Sayers SP. Efficacy of a tart cherry juice blend in preventing the symptoms of muscle damage. Br J Sports Med. 2006 Aug;40(8):679-83; discussion 683. doi: 10.1136/bjsm.2005.025429. Epub 2006 Jun 21.
PubMed 16790484Blomstrand E, Eliasson J, Karlsson HK, Kohnke R. Branched-chain amino acids activate key enzymes in protein synthesis after physical exercise. J Nutr. 2006 Jan;136(1 Suppl):269S-73S. doi: 10.1093/jn/136.1.269S.
PubMed 16365096Impellizzeri FM, Maffiuletti NA. Convergent evidence for construct validity of a 7-point likert scale of lower limb muscle soreness. Clin J Sport Med. 2007 Nov;17(6):494-6. doi: 10.1097/JSM.0b013e31815aed57.
PubMed 17993794Laurent CM, Green JM, Bishop PA, Sjokvist J, Schumacker RE, Richardson MT, Curtner-Smith M. A practical approach to monitoring recovery: development of a perceived recovery status scale. J Strength Cond Res. 2011 Mar;25(3):620-8. doi: 10.1519/JSC.0b013e3181c69ec6.
PubMed 20581704
Study at a glance
From the registry record, last updated by the sponsor on 8 Jul 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Effects of Liquid IV's Multi-Ingredient Recovery Supplement, on Muscle Recovery Following Exercise: A Randomized Crossover Study
- ClinicalTrials.gov ID
- NCT07224867
- Acronym / sponsor ID
- Pro00089373
- Conditions
- Muscle Damage, Strength Recovery, Perceived Recovery, Perceived Muscle Soreness, Physical Performance, Muscle Recovery
- Keywords
- muscle damage, muscle recovery, strength recovery, physical performance, perceived recovery, perceived muscle soreness
- Study type
- Interventional
- Recruitment status
- WithdrawnReason stopped: sponsor decided not to move forward with study
- Enrolment
- 0 participants (actual)
- Lead sponsor
- Applied Science & Performance InstituteIndustry (pharma, biotech, devices)
- Study start
- 1 Oct 2026 (estimated)
- Primary completion
- 1 Dec 2026 (estimated)
- Study completion
- 31 Jan 2027 (estimated)
- First posted
- 5 Nov 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The purpose of this study is to evaluate the efficacy of a multi-ingredient recovery supplement . The research will focus on strength recovery and performance maintenance assessing reductions in soreness and inflammation, as well as muscle repair and damage mitigation. By investigating these key recovery metrics, this study aims to investigate the efficacy of this supplement as a solution for active individuals seeking to optimize post-exercise recovery.
Study design
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Double blind (participant, investigator)
- Number of arms
- 2
- Model description
- randomized, double-blind, crossover study
| Arm | Type | Receives |
|---|---|---|
| proprietary post workout recovery supplement | Experimental | Dietary Supplement: proprietary post workout recovery supplementLiquid I.V. post workout recovery powder mixture |
| Control Treatment | Placebo comparator | Dietary Supplement: Control placeboinactive/inert placebo mixture |
Who may qualify
- Ages
- 18 to 55 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (7 listed)
- Healthy males or females aged 18-55 years old.
- Must be recreationally trained or trained/developmental as previously defined (McKay et al., 2021).
- Able to read and write in English.
- Cessation of current use of tart cherry extract, protein powders, essential amino acids/BCAAs, calcium fructoborate, boron, or anabolic agents for a minimum of 1 week prior to, and throughout the duration of the study.
- Willing to maintain regular supplement routine otherwise during the trial, and will not introduce or discontinue a supplement, other than what was previously mentioned.
Key exclusion criteria (17 listed)
- Sedentary
- Highly trained/national level or professional athlete
- Known diagnosis of any cardiovascular, metabolic, musculoskeletal, endocrine, or renal disease/disorder.
- Recent musculoskeletal injury (<3-months)
- Recent orthopedic surgery (<12-months)
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| proprietary post workout recovery supplementproprietary post workout recovery supplement | Dietary supplement | proprietary post workout recovery supplement |
| Control placeboinactive/inert placebo mix | Dietary supplement | Control Treatment |
Primary outcome
Lower Body Power and Force Generation (Maximal Countermovement Jump (CMJ))
Time frame: Taken at baseline testing, 24 hour, 48 hour, and 72 hour.
The force plate platform consists of two force plates (left \& right) containing four force sensors which measure ground reaction force. The sensors are connected to a laptop and analyzed using Mechanography GRFP Research Edition® software (version 4.2-b05.53-RES). Subjects will perform three maximal vertical CMJs on a calibrated force plate. Subjects will be instructed to stand upright on the center of the force plate with feet shoulder-width apart and hands placed on the hips. Subjects will perform a maximal effort vertical jump with proper form and instructed to land with soft knees and remain still after each jump for data capture. A 30sec rest period will be provided between each trial. Data will be used to assess maximum force output (Fmax), rate of force development (RFD), jump height (inches), and maximum power (Pmax). Force curves will be reviewed for each trial, and results averaged. This will quantify full-body mechanical power under standardized, reproducible conditions.
Lower-Body Strength (Biodex Dynamometry)
Time frame: Tested at baseline, 24 hour, 48 hour, and 72 hour.
Participants will complete isometric and isokinetic strength assessments, unilaterally on the dominant leg, of knee extension (ext) and flexion (flx) using a Biodex System 4 Pro dynamometer (Biodex Medical Systems, Shirley, NY). Proper positioning and stabilization will be ensured per manufacturer guidelines. Isometric testing - knee joint will be fixed @60° of flexion. Participants will perform 3 maximal effort ext/flx contractions, each lasting 5sec, with 5sec of rest between efforts. Highest recorded torque value across the 3 trials will be analyzed. Subjects will then complete isokinetic testing consisting of 5 maximal concentric knee ext and flx at an angular velocity of 60°/s. Full range of motion will be established prior to testing. Peak torque and average torque across all 5 repetitions will be recorded for analysis. All torque values will be gravity-corrected by the system software, and same technician will conduct all tests for consistency.
Perceived Recovery Status (PRS) Likert Scale
Time frame: Taken at baseline testing, 24 hours, 48 hours, and 72 hours timepoints.
The PRS is a composite scale directly proportional to recovery (Laurent, et al. 2011). Hence, the purpose of the scale will be to assess the participant's recovery from the previous exercise session. Subjects will be informed that having no soreness and a high feeling of recovery is a 10, while extreme soreness and low feelings of recovery, leading to limited ability to function, is defined by a range of 0-3, adequate recovery, with some ability to function, is defined by a range of 4-7, and good recovery, with the ability to function normally, is defined by a range of 8-10.
Perceived Body Soreness Likert Scale
Time frame: Taken at baseline testing, 24 hour, 48 hour, and 72 hour timepoints.
A 7-point likert scale will be used for lower body muscle soreness, after performing 5 air squats. The Likert scale is a validated instrument designed to assess self reported muscle soreness, measured across a 7-point scale. The scale ranges from 0 (no soreness), to 6 (unbearable soreness), and has good validity and reliability (Impellizzeri, F., et al., 2007).
Numeric Pain Rating Scale (NPRS)
Time frame: NPRS will be taken at baseline, 24 hour, 48 hour and 72 hour time points
The NPRS is a subjective 11-point numeric pain rating scale, with 0 representing "no pain" and 10 representing "unbearable pain." The Likert scale is a validated instrument designed to assess self reported pain. For this study, the subjects will be asked to assign a number to their average lower extremity joint pain (hip and knee joint pain) (Hawker et al., 2011) at the designated time points.
1 secondary outcome
Body Hydration Status (InBody)
Time frame: taken during baseline testing only - before exercise and again at ~10 minutes post treatment
Results and safety
The study was withdrawn before enrolling participants, so there are no results.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 8 Jul 2026.
- Background: PubMed 21574783Skurvydas A, Brazaitis M, Venckunas T, Kamandulis S. Predictive value of strength loss as an indicator of muscle damage across multiple drop jumps. Appl Physiol Nutr Metab. 2011 Jun;36(3):353-60. doi: 10.1139/h11-023. Epub 2011 May 16.
- Background: PubMed 16424141Shimomura Y, Yamamoto Y, Bajotto G, Sato J, Murakami T, Shimomura N, Kobayashi H, Mawatari K. Nutraceutical effects of branched-chain amino acids on skeletal muscle. J Nutr. 2006 Feb;136(2):529S-532S. doi: 10.1093/jn/136.2.529S.
- Background: PubMed 16790484Connolly DA, McHugh MP, Padilla-Zakour OI, Carlson L, Sayers SP. Efficacy of a tart cherry juice blend in preventing the symptoms of muscle damage. Br J Sports Med. 2006 Aug;40(8):679-83; discussion 683. doi: 10.1136/bjsm.2005.025429. Epub 2006 Jun 21.
- Background: PubMed 16365096Blomstrand E, Eliasson J, Karlsson HK, Kohnke R. Branched-chain amino acids activate key enzymes in protein synthesis after physical exercise. J Nutr. 2006 Jan;136(1 Suppl):269S-73S. doi: 10.1093/jn/136.1.269S.
- Background: PubMed 17993794Impellizzeri FM, Maffiuletti NA. Convergent evidence for construct validity of a 7-point likert scale of lower limb muscle soreness. Clin J Sport Med. 2007 Nov;17(6):494-6. doi: 10.1097/JSM.0b013e31815aed57.
- Background: PubMed 20581704Laurent CM, Green JM, Bishop PA, Sjokvist J, Schumacker RE, Richardson MT, Curtner-Smith M. A practical approach to monitoring recovery: development of a perceived recovery status scale. J Strength Cond Res. 2011 Mar;25(3):620-8. doi: 10.1519/JSC.0b013e3181c69ec6.
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Eric Sikorski, PhD principal investigator
Applied Science and Performance Institute
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing proprietary post workout recovery supplement in people with Muscle Damage and Strength Recovery, run by Applied Science & Performance Institute at 1 site in United States.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 7 inclusion and 17 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: withdrawn, as of the sponsor's last update on 8 Jul 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in United States. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study was withdrawn before enrolling participants, so there are no results.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Lower Body Power and Force Generation (Maximal Countermovement Jump (CMJ)) (time frame: Taken at baseline testing, 24 hour, 48 hour, and 72 hour.). The study lists 5 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
On the ClinicalTrials.gov record for NCT07224867, which also lists 6 linked publications.
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 8 Jul 2026.