Metabolic StressTEAMNCT07250555
Analysis of Bioenergetics and Fatigue Responses in Basketball (TEAM)
A study testing BASKETBALL GAME in people with Metabolic Stress, run by University of Thessaly at 1 site in Greece.
Trial summary
The aims of the study are: 1. To examine skeletal muscle and blood metabolites during an official basketball game and to relate those to skeletal muscle performance during and after the game. 2. To investigate whether skeletal muscle and blood metabolites are related to game-induced fatigue. 3. Read more about this study
- Who is it for?
- Ages 18 to 30 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Metabolic Stress
- What is being tested?
- BASKETBALL GAME
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 30 participants
- Where
- 1 siteGreece
- Study dates
- 30 May 2025to 28 Feb 2026
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 30 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Health status: were free of recent musculoskeletal injuries and/or illnesses (≥ 8 months before the study).
- Not consuming ergogenic supplements/medications (≥ 6 months before the study)
- Not smokers
Who cannot join
- Completion of the pre-determined participation time during the experimental game.
- Completion of all field measurements.
- Completion of all pre-determined sampling points for biological tissue.
Trial locations and contacts
1 site in 1 country. Contact the study team or a site near you to ask about taking part.
Greece1 site, 1 recruiting
- Recruiting
University of Thessaly, Department of Physical Education and Sports Science
Trikala, Thessaly, 42100
Ioannis G Fatouros, Professor, +30 24310 47047, ifatouros@uth.gr
Athanasios Chatzinikolaou, Professor, +30 25310 39632, achatzin@phyed.duth.gr
About this study
Official title: sTudy of the bioEnergetics and fAtigue Responses of a Basketball gaMe: The TEAM Trial
The aims of the study are:
- To examine skeletal muscle and blood metabolites during an official basketball game and to relate those to skeletal muscle performance during and after the game.
- To investigate whether skeletal muscle and blood metabolites are related to game-induced fatigue.
- To relate field activity during a basketball game with the responses of skeletal muscle and blood metabolites.
- To investigate how blood lactate reflects muscle lactate concentration during a basketball game.
- To determine whether temporary fatigue develops during a basketball game. game.
Read the detailed description
The basketball players will participate in three experimental games (N = 10/game) on an indoors basketball stadium. During a 2-week familiarization period, volunteers will be familiarized with the experimental procedures and participate in very light training (at local basketball facilities) aimed at developing match tactics and team cohesion. At the end of the familiarization period and before the experimental game, participants will undergo a 3-day baseline performance testing (resting conditions) at University (Department of Physical Education and Sport Sciences, University of Thessaly) facilities. Prior to the games, the players will refrain from strenuous exercise and intake of alcohol for 48 hours and from tobacco and caffeine for 12 h. Dietary intake will be monitored with daily diet recalls for 7 days before the experimental period and will be standardized for all participants by a registered dietitian. The dietitian will also prescribe the meals and snacks on the game-day. On the day of the game, participants will arrive at the stadium 1.5 h before for resting skeletal muscle and blood sampling. For the first muscle biopsy and in preparation for later obtainment of biopsies from vastus lateralis muscle. An incision will be made under local anesthesia (20 mg/ml xylocaine without adrenalin) and covered by sterile band-aid strips and a thigh and shoulder bandages. In addition, an inertial measurement unit (IMU) and a heart rate monitor will be placed on each subject. During the match, a number of physiological measurements will be performed at fixed times (before the game, at half-time and post-match) and after intense exercise periods within the four 10-min periods of the game. Volunteers will be randomly assigned, using the sealed envelope procedure, in six teams representing all field positions and each team will play in one of three experimental games (random allocation using standard procedures) against each other in a full 40-minute game according to official regulations. A total of 10 additional players (from various playing positions) will serve as substitute players that could enter the pitch for a few minutes when a player will have to leave the court (either participate in measurements or will have completed it's pre-determined playing time or in case of an injury). Fifteen players (pre/post game measurement group) will have muscle and blood samples taken at rest, at the end of the 2nd period (half-time) and at the end of the 4th period (end of game). A second group of eight players (intense periods measurement group 1) will have muscle and blood samples collected at rest and during the first and third period of the game (immediately after an intense period of game-play). A third group of seven players (intense periods measurement group 1) will have muscle and blood samples collected at rest and during the second and fourth period of the game (immediately after an intense period of game-play). An intense exercise period of game-play is identified as a period with a heart rate of > 90% of maximal [as assessed by the maximal heart rate obtained during a graded exercise test to measure the maximal oxygen consumption (VO2max)], a high number of high-intensity runs (speed of > 18 km/h), sprints (speed of > 25 km/h) and/or jumps evaluated by a professional licensed coach. All muscle biopsies will be sampled from the vastus lateralis. All players will have their field activity and heart rate measured throughout the game using IMU units and heart rate monitors. Games will start at 17.00 - 18 p.m. to avoid diurnal variations and will be held under normal environmental conditions. A standardized 30-min warm-up period will be performed before each game. All games will be videotaped.
- Conditions
- Metabolic Stress
- Being tested
- BASKETBALL GAME
- Keywords
- basketball, performance, blood metabolites, skeletal muscle metabolism
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
BASKETBALL GAMEOther
Participants will arrive 1.5 hours pre-game for initial vastus lateralis muscle biopsies and blood sampling under local anesthesia (20 mg/ml xylocaine without adrenalin). They will be fitted with IMU sensors and heart rate monitors. Volunteers are randomly assigned to teams(6 teams) for a full 40-minute game. During the match, physiological measurements will be performed at fixed times (pre, at half-time, and post-match) and after intense exercise periods (HR>90% max, high-speed runs>18km/h) within the four 10-min periods. Three groups provide samples: Group 1 (n=15): rest, half-time, end-game. Group 2 (n=8): rest, post-intense period in 1st/3rd periods. Group 3 (n=7): rest, post-intense period in 2nd/4th periods. Ten players serve as substitutes. Games will start at 17.00 - 18 p.m. to avoid diurnal variations and will be held under normal environmental conditions. A standardized 30-min warm-up period will be performed before each game.
| Study group | Role | What they receive |
|---|---|---|
| BASKETBALL GROUP | Experimental | Not described Receives: Other: BASKETBALL GAME |
What is being measured
The primary outcome is the main question the study is designed to answer.
Measurement of countermovement jump height
Will be measured using a force platform system
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Bilateral strength peak force and rate of force development on mid-thigh pull
Will be measured using a dynamometer.
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of maximal sprinting speed over 5 meters
5-m sprinting speed will be assessed on the basketball court using infrared photocells
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of maximal sprinting speed over 20 meters
20-m sprinting speed will be assessed on the basketball court using infrared photocells
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of the capacity to perform repeated sprints
5 x 20m repeated maximal sprints will be performed with 25 sec rest in between. Speed will be recorded using infrared photocells
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of field activity variables during the basketball game
Field activity will be continuously recorded during the basketball game using a microsensor inertial measurement unit (Kinexon Perform IMU).
Measured: Continuous monitoring during each 40-minute game.
Measurement of heart rate during the basketball game ad recording the average and maximal heart rate of the game.
Field activity will be continuously recorded during the basketball game using a Team Polar Pro heart rate monitor.
Measured: Continuous monitoring during each 40-minute game.
Measurement of skeletal muscle glycogen content
Will be assessed on muscle biopsy samples. Muscle glycogen content will be determined spectrophotometrically.
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of skeletal muscle fiber-type distribution and fiber-type- specific glycogen content.
Will be assessed on muscle biopsy samples by histochemical analysis
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of skeletal muscle phosphocreatine levels
Will be assessed on muscle biopsy samples by a flurometric assay.
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of skeletal muscle lactate
Will be assessed on muscle biopsy samples by a flurometric assay.
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of effort and fatigue
Will be measured by using the Borg Rating of Perceived Exertion (RPE) scale
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Secondary outcomes (24)
Body mass
Body mass will be measured using a beam balance
Measured: Change from baseline (pre-game) to 40 minutes of the game
Body height
Body height will be measured using a beam balance with a stadiometer
Measured: At baseline
Body mass index (BMI)
BMI will be calculated using the Quetelet's equation
Measured: At baseline
Body fat (BF)
Body fat (%) will be assessed by whole-body dual-energy X-ray absorptiometry (DXA)
Measured: At baseline
Fat mass (FM)
FM (kg) will be assessed by whole-body dual-energy X-ray absorptiometry (DXA)
Measured: At baseline
Fat-free mass (FFM)
FFM (kg) will be assessed by whole-body dual-energy X-ray absorptiometry (DXA)
Measured: At baseline
Bone mass density
Bone mass density will be measured by using Dual-emission X-ray absorptiometry
Measured: At baseline
Bone mass content
Bone mass content will be measured by using Dual-emission X-ray absorptiometry
Measured: At baseline
Dietary intake
Dietary intake will be assesed over a 7-day period using diet recalls
Measured: At baseline
Evaluation of physical activity (PA)
International Physical Activity Questionnaire - IPAQ
Measured: At baseline
Rest Heart Rate
The rest heart rate will be estimated using a heart rate monitor
Measured: At baseline
Peak Maximal oxygen consumption (Peak VO2)
Peak VO2 will be estimated by open circuit spirometry via breath by breath method
Measured: At baseline
Basketball-specific conditioning (endurance)
Yo-Yo intermittent endurance test 1
Measured: At baseline
Basketball-specific conditioning (recovery)
Yo-Yo intermittent recovery test 1
Measured: At baseline
Concentric and eccentric isokinetic peak torque of knee extensors and flexors of both dominant and non-dominant limbs
Will be measured at 60°/s using a three-repetition protocol via an isokinetic dynamometer
Measured: At baseline
Maximal heart rate
Will be measured using an automated online pulmonary gas exchange system via breath- by- breath analysis during a graded exercise testing on a treadmill
Measured: Change from baseline (pre-game) to that recorded during the 40-minute game
Measurement of blood lactate
Will be measured in capillary blood collected from the index finger using a handheld portable analyzer
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of plasma glucose concentration
Will be measured using a Clinical Chemistry Analyzer and a commercially available kit
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of blood Glycerol concentration
Will be measured using a commercially available kit using a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric/fluorometric product proportional to the glycerol present.
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of Plasma ammonia concentration
Will be quantitatively determined on an analyzer using a commercially available kit
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of Plasma potassium concentration
Will be measured using a flame photometer (Radiometer FLM3), with lithium as the internal standard
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of Plasma- free fatty acid
Will be measured spectophotometrically using an enzymatic kit
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of blood hemoglobin and hematocrit
Will be measured using an automated hematology analyzer
Measured: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of fluid loss and intake
Will be measured using the measures of body mass (using a digital weight scale) and water consumption during a game.
Measured: Change from baseline (pre-game) to 40 minutes of the game
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted26 Nov 2025
- Study start30 May 2025
- Primary completion30 Nov 2025 (estimated)
- Study completion28 Feb 2026 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Metabolic StressStudies of the same condition, open and under way.
- More trials run by University of ThessalyThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Enrolment
- 30 participants (estimated)
Sponsor and investigators
- Lead sponsor
- University of Thessaly
- Sponsor type
- University, hospital or other
- Collaborators
- University of Southern Denmark, Democritus University of Thrace
- Responsible party
- Principal investigator, Ioannis G. Fatouros, University of Thessaly
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07250555
- Sponsor's protocol ID
- The TEAM trial
- Acronym
- TEAM
- Status last verified
- Aug 2025
- First submitted
- 28 Aug 2025
- Last updated
- 26 Nov 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Study at a glance
From the registry record, last updated by the sponsor on 26 Nov 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- sTudy of the bioEnergetics and fAtigue Responses of a Basketball gaMe: The TEAM Trial
- ClinicalTrials.gov ID
- NCT07250555
- Acronym / sponsor ID
- TEAM / The TEAM trial
- Conditions
- Metabolic Stress
- Keywords
- basketball, performance, blood metabolites, skeletal muscle metabolism
- Study type
- Interventional
- Recruitment status
- Recruiting
- Enrolment
- 30 participants (estimated)
- Lead sponsor
- University of ThessalyUniversity, hospital or other
- Collaborators
- University of Southern Denmark, Democritus University of Thrace
- Study start
- 30 May 2025 (actual)
- Primary completion
- 30 Nov 2025 (estimated)
- Study completion
- 28 Feb 2026 (estimated)
- First posted
- 26 Nov 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The aims of the study are:
- To examine skeletal muscle and blood metabolites during an official basketball game and to relate those to skeletal muscle performance during and after the game.
- To investigate whether skeletal muscle and blood metabolites are related to game-induced fatigue.
- To relate field activity during a basketball game with the responses of skeletal muscle and blood metabolites.
- To investigate how blood lactate reflects muscle lactate concentration during a basketball game.
- To determine whether temporary fatigue develops during a basketball game. game.
Detailed description
The basketball players will participate in three experimental games (N = 10/game) on an indoors basketball stadium. During a 2-week familiarization period, volunteers will be familiarized with the experimental procedures and participate in very light training (at local basketball facilities) aimed at developing match tactics and team cohesion. At the end of the familiarization period and before the experimental game, participants will undergo a 3-day baseline performance testing (resting conditions) at University (Department of Physical Education and Sport Sciences, University of Thessaly) facilities. Prior to the games, the players will refrain from strenuous exercise and intake of alcohol for 48 hours and from tobacco and caffeine for 12 h. Dietary intake will be monitored with daily diet recalls for 7 days before the experimental period and will be standardized for all participants by a registered dietitian. The dietitian will also prescribe the meals and snacks on the game-day. On the day of the game, participants will arrive at the stadium 1.5 h before for resting skeletal muscle and blood sampling. For the first muscle biopsy and in preparation for later obtainment of biopsies from vastus lateralis muscle. An incision will be made under local anesthesia (20 mg/ml xylocaine without adrenalin) and covered by sterile band-aid strips and a thigh and shoulder bandages. In addition, an inertial measurement unit (IMU) and a heart rate monitor will be placed on each subject. During the match, a number of physiological measurements will be performed at fixed times (before the game, at half-time and post-match) and after intense exercise periods within the four 10-min periods of the game. Volunteers will be randomly assigned, using the sealed envelope procedure, in six teams representing all field positions and each team will play in one of three experimental games (random allocation using standard procedures) against each other in a full 40-minute game according to official regulations. A total of 10 additional players (from various playing positions) will serve as substitute players that could enter the pitch for a few minutes when a player will have to leave the court (either participate in measurements or will have completed it's pre-determined playing time or in case of an injury). Fifteen players (pre/post game measurement group) will have muscle and blood samples taken at rest, at the end of the 2nd period (half-time) and at the end of the 4th period (end of game). A second group of eight players (intense periods measurement group 1) will have muscle and blood samples collected at rest and during the first and third period of the game (immediately after an intense period of game-play). A third group of seven players (intense periods measurement group 1) will have muscle and blood samples collected at rest and during the second and fourth period of the game (immediately after an intense period of game-play). An intense exercise period of game-play is identified as a period with a heart rate of > 90% of maximal [as assessed by the maximal heart rate obtained during a graded exercise test to measure the maximal oxygen consumption (VO2max)], a high number of high-intensity runs (speed of > 18 km/h), sprints (speed of > 25 km/h) and/or jumps evaluated by a professional licensed coach. All muscle biopsies will be sampled from the vastus lateralis. All players will have their field activity and heart rate measured throughout the game using IMU units and heart rate monitors. Games will start at 17.00 - 18 p.m. to avoid diurnal variations and will be held under normal environmental conditions. A standardized 30-min warm-up period will be performed before each game. All games will be videotaped.
Study design
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Number of arms
- 1
| Arm | Type | Receives |
|---|---|---|
| BASKETBALL GROUP | Experimental | Other: BASKETBALL GAME |
Who may qualify
- Ages
- 18 to 30 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (3 listed)
- Health status: were free of recent musculoskeletal injuries and/or illnesses (≥ 8 months before the study).
- Not consuming ergogenic supplements/medications (≥ 6 months before the study)
- Not smokers
Key exclusion criteria (3 listed)
- Completion of the pre-determined participation time during the experimental game.
- Completion of all field measurements.
- Completion of all pre-determined sampling points for biological tissue.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| BASKETBALL GAMEParticipants will arrive 1.5 hours pre-game for initial vastus lateralis muscle biopsies and blood sampling under local anesthesia (20 mg/ml xylocaine without adrenalin). They will be fitted with IMU sensors and heart rate monitors. Volunteers are randomly assigned to teams(6 teams) for a full 40-minute game. During the match, physiological measurements will be performed at fixed times (pre, at half-time, and post-match) and after intense exercise periods (HR>90% max, high-speed runs>18km/h) within the four 10-min periods. Three groups provide samples: Group 1 (n=15): rest, half-time, end-game. Group 2 (n=8): rest, post-intense period in 1st/3rd periods. Group 3 (n=7): rest, post-intense period in 2nd/4th periods. Ten players serve as substitutes. Games will start at 17.00 - 18 p.m. to avoid diurnal variations and will be held under normal environmental conditions. A standardized 30-min warm-up period will be performed before each game. | Other | BASKETBALL GROUP |
Primary outcome
Measurement of countermovement jump height
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be measured using a force platform system
Bilateral strength peak force and rate of force development on mid-thigh pull
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be measured using a dynamometer.
Measurement of maximal sprinting speed over 5 meters
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
5-m sprinting speed will be assessed on the basketball court using infrared photocells
Measurement of maximal sprinting speed over 20 meters
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
20-m sprinting speed will be assessed on the basketball court using infrared photocells
Measurement of the capacity to perform repeated sprints
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
5 x 20m repeated maximal sprints will be performed with 25 sec rest in between. Speed will be recorded using infrared photocells
Measurement of field activity variables during the basketball game
Time frame: Continuous monitoring during each 40-minute game.
Field activity will be continuously recorded during the basketball game using a microsensor inertial measurement unit (Kinexon Perform IMU).
Measurement of heart rate during the basketball game ad recording the average and maximal heart rate of the game.
Time frame: Continuous monitoring during each 40-minute game.
Field activity will be continuously recorded during the basketball game using a Team Polar Pro heart rate monitor.
Measurement of skeletal muscle glycogen content
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be assessed on muscle biopsy samples. Muscle glycogen content will be determined spectrophotometrically.
Measurement of skeletal muscle fiber-type distribution and fiber-type- specific glycogen content.
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be assessed on muscle biopsy samples by histochemical analysis
Measurement of skeletal muscle phosphocreatine levels
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be assessed on muscle biopsy samples by a flurometric assay.
Measurement of skeletal muscle lactate
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be assessed on muscle biopsy samples by a flurometric assay.
Measurement of effort and fatigue
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Will be measured by using the Borg Rating of Perceived Exertion (RPE) scale
24 secondary outcomes
Body mass
Time frame: Change from baseline (pre-game) to 40 minutes of the game
Body height
Time frame: At baseline
Body mass index (BMI)
Time frame: At baseline
Body fat (BF)
Time frame: At baseline
Fat mass (FM)
Time frame: At baseline
Fat-free mass (FFM)
Time frame: At baseline
Bone mass density
Time frame: At baseline
Bone mass content
Time frame: At baseline
Dietary intake
Time frame: At baseline
Evaluation of physical activity (PA)
Time frame: At baseline
Rest Heart Rate
Time frame: At baseline
Peak Maximal oxygen consumption (Peak VO2)
Time frame: At baseline
Basketball-specific conditioning (endurance)
Time frame: At baseline
Basketball-specific conditioning (recovery)
Time frame: At baseline
Concentric and eccentric isokinetic peak torque of knee extensors and flexors of both dominant and non-dominant limbs
Time frame: At baseline
Maximal heart rate
Time frame: Change from baseline (pre-game) to that recorded during the 40-minute game
Measurement of blood lactate
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of plasma glucose concentration
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of blood Glycerol concentration
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of Plasma ammonia concentration
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of Plasma potassium concentration
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of Plasma- free fatty acid
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of blood hemoglobin and hematocrit
Time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).
Measurement of fluid loss and intake
Time frame: Change from baseline (pre-game) to 40 minutes of the game
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 26 Nov 2025.
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing BASKETBALL GAME in people with Metabolic Stress, run by University of Thessaly at 1 site in Greece.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 3 inclusion and 3 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: looking for participants now, as of the sponsor's last update on 26 Nov 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Greece. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
Contact Ioannis G Fatouros, Professor (+302431047047, ifatouros@uth.gr), or a site near the patient. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Measurement of countermovement jump height (time frame: Change from baseline to the: a. 20th minute (half-time) and 40th minute (end of the game) (N=10), b. 5th minute (mid-1st period) and 25th minute (mid-3rd period) (N=10) and c. 15th minute (mid-2nd period) and 35th minute (mid-4th period) (N=10).). The study lists 12 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 26 Nov 2025.