AUGMENTED RESPONSE OF VOLATILE BIOMARKERS IN THE ASSESSMENT OF OESOPHAGOGASTRIC CANCER (AROMA2)

Oesophagogastric Cancer Early Detection: A Clinical Challenge

The detection of oesophagogastric cancer at an early stage remains an unmet clinical need. It's incidence in the UK and many other western countries has risen dramatically in recent years. Challenges in diagnosis are attributable to the largely non-specific nature of its early symptoms, which overlap with benign gastrointestinal conditions, alongside the resource intense and invasive nature of the current diagnostic pathways such as endoscopy. The so called 'red flag' symptoms that prompt more urgent investigation are often associated with advanced disease where curative treatments are limited. As a result, the five-year survival rate for oesophagogastric cancer in the UK remains distressingly low at 15-20%, one of the poorest in Europe. Early detection offers the best chance for effective intervention, where minimally invasive treatments and surgical procedures can lead to survival rates of over 85%.

There is a growing body of evidence that volatile organic compounds (VOCs), which are small molecules exhaled in breath, hold promise as non-invasive biomarkers for cancer detection in the form of a breath test. Our work suggests that VOCs are produced by both cancer cells and the tumour-associated microbiome. Our understanding of the pathways leading to VOC production allow us to propose augmentation of the breath test using certain nutrients to increase the production of volatile markers and improve the tests accuracy.

We have developed a breath test for diagnosing oesophagogastric cancer, starting with volatile biomarker discovery and progressing to a multi-centre NIHR-funded clinical study which demonstrated a sensitivity of 80% and specificity of 81%. To explore the origin of VOCs in oesophagogastric cancer, we developed an in-vivo human model that allowed for the compartmental measurement of VOCs from the gastrointestinal tract, airways, and mixed breath during general anaesthesia. The comparable levels of volatile fatty acids in both mixed and bronchial breath from cancer patients suggest that the primary source of these VOCs is the lungs, likely entering the breath through the systemic circulation.

Alongside this we have developed standardised operating procedures for VOC analysis with a platform for high-throughput breath analysis adherent to ISO accreditation standards. Our validated methodology has a VOC quantitation limit of 1.25 ng/L with established quality assurance and control procedures.

The AROMA programme is a comprehensive initiative aimed at understanding the pathways underpinning cancer-derived VOC production. Instead of only focusing on improving the sensitivity of detection equipment, our proposed solution involves augmenting the production of VOCs by introducing simple nutrients. These nutrients are anticipated to be metabolised rapidly by cancer cells and the cancer-associated microbiome to augment cancer-specific VOC production. By exploiting these VOC-pathways we will develop a breath test for oesophagogastric cancer that has a clinically acceptable sensitivity and specificity. After conducting optimisation experiments in a proof of principal study (AMNED; REC Ref:18/LO/0078), we developed an oral stimulant drink (OSD) composed of glucose, glycerol, amino acids and iron. The breath of patients with oesophagogastric cancer(n=30) and controls (n=30) was collected for 2 hours at 30-minute intervals after consuming the OSD. The peak VOC response varied but occurred within an hour of consumption. We also showed that VOC production by oesophagogastric cancer-associated bacteria could be enhanced using the same nutrient substrates.

In the AROMA1 study (n=648), a cancer detection model based on a breath test, augmented by an OSD, was developed. The AROMA Bioresource (n=335) established a comprehensive bioresource of tissue from cancer and non-cancer patients to understand pathways leading to VOC origin.

Justification for AROMA2

The AROMA2 study is a pivotal next step in this programme. AROMA2 will test the breath test in a multi-centre triple-blinded clinical trial, evaluating its sensitivity and specificity in patients referred to the upper gastrointestinal (GI) cancer pathway. These patients present with symptoms suggestive of upper GI cancer but have an unknown diagnosis. It will provide essential validation of the breath test in a real-world clinical setting, to establish the test's diagnostic accuracy in a clinical context.

Methods

At least 5400 individuals with suspected upper GI cancer, referred from primary care along upper gastrointestinal cancer pathways, will be recruited from 20+ centres. The aim is to have 216 cancers and 5184 controls with complete dataset (reliable breath data and results of reference test). Breath samples, before and after an oral stimulant drink (OSD), will be collected on thermal desorption tubes which will be analysed using gas chromatography-mass spectrometry (GC-MS). The diagnostic accuracy of breath test to detect cancer will be tested.

Anticipated impact and dissemination: The non-invasive breath test will provide direct patient benefit through earlier and accurate detection of Upper GI cancers, along with higher patient acceptability. Ensuring timely referral will translate to curative treatment and improved long-term survival. Outcomes will be disseminated via conference presentations, publications, the local public advisory group (PAG), charities, the local research website and social media.