Evaluate the Fluorescence of Natural Tooth Following DehydrationNCT07295977
Effect of Dehydration on Dental Fluorescence
An observational study of Evaluate the Fluorescence of Natural Tooth Following Dehydration, run by Marmara University at 1 site in Turkey (Türkiye).
Trial summary
A cheek retractor will be placed to ensure consistent isolation throughout the procedure. Dehydration will be assessed at baseline, at the 5th minute, and at the 10th minute using three different evaluation techniques. Read more about this study
- Who is it for?
- Ages 18 to 35 yearsHealthy volunteers can take part
- Conditions the study is for
- Evaluate the Fluorescence of Natural Tooth Following Dehydration
- What is being tested?
- Digital photograph with Ashur plaque indicator, Digital photographs with the Led lights (365 nm) and The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
- How many people
- 10 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 7 Apr 2025to Dec 2025
- How long will I take part?
- 1 Day
- Phase
- Observational
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 35 years
- All sexes
- Healthy volunteers accepted
- Adult
Study population: Individuals with caries-free teeth, no enamel defects, no restorations on tooth surfaces, no visible plaque, and adequate cognitive ability to read and understand the informed consent form will be included in the study. Those with teeth exhibiting discoloration or with systemic conditions or medication use that may cause structural changes on the tooth surfaces will be excluded. Maxillary and mandibular anterior teeth will be evaluated.
Who may be able to join
- Individuals with caries-free teeth, no enamel defects, no restorations on tooth surfaces, no visible plaque, and adequate cognitive ability to read and understand the informed consent form.
Who cannot join
- Individuals with teeth exhibiting discoloration or with systemic conditions or medication use that may cause structural changes on the tooth surfaces.
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Marmara University Faculty of Dentistry
Istanbul
About this study
Official title: The Effect of Dehydration on Natural Dental Fluorescence: A Clinical Evaluation
A cheek retractor will be placed to ensure consistent isolation throughout the procedure. Dehydration will be assessed at baseline, at the 5th minute, and at the 10th minute using three different evaluation techniques. At each time point, digital photographs will be captured under two distinct fluorescence light sources to document changes visually. In addition, fluorescence images will be obtained using the QLF (Quantitative Light-Induced Fluorescence) device to provide objective, quantitative data. All images will be recorded and analyzed digitally to evaluate dehydration-related changes in fluorescence. The aim of our study is to clinically evaluate the changes in the fluorescence properties of natural tooth following dehydration.
Read the detailed description
According to the power analysis, the required number of teeth was 69, with 95% statistical power and a 5% alpha error level. The study will be conducted at the Clinic of the Department of Restorative Dentistry, Marmara University Faculty of Dentistry. Individuals with caries-free teeth, no enamel defects, no restorations on tooth surfaces, no visible plaque, and adequate cognitive ability to read and understand the informed consent form will be included in the study. Those with teeth exhibiting discoloration or with systemic conditions or medication use that may cause structural changes on the tooth surfaces will be excluded. Maxillary and mandibular anterior teeth will be evaluated.
A cheek retractor will be placed in the patients' mouths, and baseline, 5th-minute, and 10th-minute dehydration will be evaluated using three different techniques. Digital photographs will be taken at baseline and post-procedure under two different fluorescence light sources (Ashur plaque indicator (405 nm), and Led lights (365 nm)), and the images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. Analyses will be carried out digitally. Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000) and for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room. QLF images will be obtained with its own camera. The photographs and the QLF images will be analyzed using Adobe Photoshop software, and color changes will be determined based on ∆E values. For each method ∆E values will be calculated individually, there will be 3 different ∆E values (baseline and 5th-minute, baseline and 10th-minute, 5th-minute and 10th-minute). For ∆E calculations, the CIEDE2000 formula will be used, employing the measured L*, a*, b* and L*, c*, h* values obtained from baseline, 5th-minute and 10th-minute images. For the digital measurements, the intraobserver variability will be assessed, and after obtaining a high level of agreement, the study will be initiated. In statistical analyses, normality will be assessed using the Shapiro-Wilk test; repeated-measures ANOVA will be used if normal distribution is present, and the Friedman test will be applied if normality is not observed. A significance level of p<0.05 will be accepted.
The aim of this clinical study is to clinically evaluate the changes in the fluorescence properties of natural teeth following dehydration.
The hypotheses of the study (H1) are as follows:
- The change in the fluorescence properties of natural teeth after dehydration exceeds clinically acceptable thresholds.
- There is a significant difference among the various fluorescence evaluation methods used in clinical practice.
- Being tested
- Digital photograph with Ashur plaque indicator, Digital photographs with the Led lights (365 nm), The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
- Keywords
- fluorescence, dehydration, teeth
- Medical subject headings
- Dehydration
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Digital photograph with Ashur plaque indicatorDiagnostic test
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Digital photographs with the Led lights (365 nm)Diagnostic test
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
The QLF (Quantitative Light-Induced Fluorescence- 405 nm) imagesDiagnostic test
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera.
| Study group | Role | What they receive |
|---|---|---|
| group 1 | Dehydration of teeth will be assessed at baseline, at the 5th minute, and at the 10th minute using three different evaluation techniques. Receives: Diagnostic Test: Digital photograph with Ashur plaque indicator; Diagnostic Test: Digital photographs with the Led lights (365 nm); Diagnostic Test: The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images |
What is being measured
The primary outcome is the main question the study is designed to answer.
Digital photograph with Ashur plaque indicator
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Measured: baseline
Digital photographs with the Led lights (365 nm)
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
Measured: baseline
The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera. All images will be captured in a dark room.
Measured: baseline
Digital photograph with Ashur plaque indicator
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Measured: at 5th minute
Digital photographs with the Led lights (365 nm)
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
Measured: at 5th minute
The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera. All images will be captured in a dark room.
Measured: at 5th minute
Digital photograph with Ashur plaque indicator
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Measured: at 10th minute
Digital photographs with the Led lights (365 nm)
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
Measured: at 10th minute
The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera. All images will be captured in a dark room.
Measured: at 10th minute
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted22 Dec 2025
- Study start7 Apr 2025
- Primary completion2 Jun 2025
- Study completionDec 2025 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Evaluate the Fluorescence of Natural Tooth Following DehydrationStudies of the same condition, open and under way.
- More trials run by Marmara UniversityThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Observational
- Observational model
- Case only
- Time perspective
- Prospective
- Enrolment
- 10 participants (estimated)
- Target duration
- 1 Day
Sponsor and investigators
- Lead sponsor
- Marmara University
- Sponsor type
- University, hospital or other
- Responsible party
- Sponsor
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07295977
- Sponsor's protocol ID
- MUDHF-2025-83
- Status last verified
- Dec 2025
- First submitted
- 8 Dec 2025
- Last updated
- 22 Dec 2025
- Results posted
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- Yes: Data may be shared after the publication of the study
Study at a glance
From the registry record, last updated by the sponsor on 22 Dec 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Effect of Dehydration on Natural Dental Fluorescence: A Clinical Evaluation
- ClinicalTrials.gov ID
- NCT07295977
- Acronym / sponsor ID
- MUDHF-2025-83
- Keywords
- fluorescence, dehydration, teeth
- Study type
- Observational
- Recruitment status
- Active, not recruiting
- Enrolment
- 10 participants (estimated)
- Lead sponsor
- Marmara UniversityUniversity, hospital or other
- Study start
- 7 Apr 2025 (actual)
- Primary completion
- 2 Jun 2025 (actual)
- Study completion
- Dec 2025 (estimated)
- First posted
- 22 Dec 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
A cheek retractor will be placed to ensure consistent isolation throughout the procedure. Dehydration will be assessed at baseline, at the 5th minute, and at the 10th minute using three different evaluation techniques. At each time point, digital photographs will be captured under two distinct fluorescence light sources to document changes visually. In addition, fluorescence images will be obtained using the QLF (Quantitative Light-Induced Fluorescence) device to provide objective, quantitative data. All images will be recorded and analyzed digitally to evaluate dehydration-related changes in fluorescence. The aim of our study is to clinically evaluate the changes in the fluorescence properties of natural tooth following dehydration.
Detailed description
According to the power analysis, the required number of teeth was 69, with 95% statistical power and a 5% alpha error level. The study will be conducted at the Clinic of the Department of Restorative Dentistry, Marmara University Faculty of Dentistry. Individuals with caries-free teeth, no enamel defects, no restorations on tooth surfaces, no visible plaque, and adequate cognitive ability to read and understand the informed consent form will be included in the study. Those with teeth exhibiting discoloration or with systemic conditions or medication use that may cause structural changes on the tooth surfaces will be excluded. Maxillary and mandibular anterior teeth will be evaluated.
A cheek retractor will be placed in the patients' mouths, and baseline, 5th-minute, and 10th-minute dehydration will be evaluated using three different techniques. Digital photographs will be taken at baseline and post-procedure under two different fluorescence light sources (Ashur plaque indicator (405 nm), and Led lights (365 nm)), and the images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. Analyses will be carried out digitally. Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000) and for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room. QLF images will be obtained with its own camera. The photographs and the QLF images will be analyzed using Adobe Photoshop software, and color changes will be determined based on ∆E values. For each method ∆E values will be calculated individually, there will be 3 different ∆E values (baseline and 5th-minute, baseline and 10th-minute, 5th-minute and 10th-minute). For ∆E calculations, the CIEDE2000 formula will be used, employing the measured L*, a*, b* and L*, c*, h* values obtained from baseline, 5th-minute and 10th-minute images. For the digital measurements, the intraobserver variability will be assessed, and after obtaining a high level of agreement, the study will be initiated. In statistical analyses, normality will be assessed using the Shapiro-Wilk test; repeated-measures ANOVA will be used if normal distribution is present, and the Friedman test will be applied if normality is not observed. A significance level of p<0.05 will be accepted.
The aim of this clinical study is to clinically evaluate the changes in the fluorescence properties of natural teeth following dehydration.
The hypotheses of the study (H1) are as follows:
- The change in the fluorescence properties of natural teeth after dehydration exceeds clinically acceptable thresholds.
- There is a significant difference among the various fluorescence evaluation methods used in clinical practice.
Study design
- Observational model
- Case only
- Time perspective
- Prospective
- Target follow-up
- 1 Day
- Number of arms
- 1
| Arm | Type | Receives |
|---|---|---|
| group 1 | – | Diagnostic Test: Digital photograph with Ashur plaque indicator, Diagnostic Test: Digital photographs with the Led lights (365 nm), Diagnostic Test: The QLF (Quantitative Light-Induced Fluorescence- 405 nm) imagesDehydration of teeth will be assessed at baseline, at the 5th minute, and at the 10th minute using three different evaluation techniques. |
Who may qualify
- Ages
- 18 to 35 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult
- Study population
- Individuals with caries-free teeth, no enamel defects, no restorations on tooth surfaces, no visible plaque, and adequate cognitive ability to read and understand the informed consent form will be included in the study. Those with teeth exhibiting discoloration or with systemic conditions or medication use that may cause structural changes on the tooth surfaces will be excluded. Maxillary and mandibular anterior teeth will be evaluated.
Key inclusion criteria (1 listed)
- Individuals with caries-free teeth, no enamel defects, no restorations on tooth surfaces, no visible plaque, and adequate cognitive ability to read and understand the informed consent form.
Key exclusion criteria (1 listed)
- Individuals with teeth exhibiting discoloration or with systemic conditions or medication use that may cause structural changes on the tooth surfaces.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Digital photograph with Ashur plaque indicatorDigital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room. | Diagnostic test | group 1 |
| Digital photographs with the Led lights (365 nm)Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room. | Diagnostic test | group 1 |
| The QLF (Quantitative Light-Induced Fluorescence- 405 nm) imagesThe images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera. | Diagnostic test | group 1 |
Primary outcome
Digital photograph with Ashur plaque indicator
Time frame: baseline
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Digital photographs with the Led lights (365 nm)
Time frame: baseline
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
Time frame: baseline
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera. All images will be captured in a dark room.
Digital photograph with Ashur plaque indicator
Time frame: at 5th minute
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Digital photographs with the Led lights (365 nm)
Time frame: at 5th minute
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
Time frame: at 5th minute
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera. All images will be captured in a dark room.
Digital photograph with Ashur plaque indicator
Time frame: at 10th minute
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Ashur plaque indicator (405 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the Ashur device (1/125, f/22, ISO 25600). To ensure standardization all images will be captured in a dark room.
Digital photographs with the Led lights (365 nm)
Time frame: at 10th minute
Digital photographs will be taken at baseline and post-procedure under the fluorescence light source (Led lights (365 nm). Images will be captured with a Sony Alpha 7iii (Sony, Japan) digital camera equipped with a 90-mm macro lens. Camera settings will be adjusted for the LED lights (1/125, f/22, ISO 8000). To ensure standardization, the LED lights will be stabilized on the flash brackets at a 45° angle on both sides, and all images will be captured in a dark room.
The QLF (Quantitative Light-Induced Fluorescence- 405 nm) images
Time frame: at 10th minute
The images obtained using the QLF (Quantitative Light-Induced Fluorescence- 405 nm) device will be recorded. QLF images will be obtained with its own camera. All images will be captured in a dark room.
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 22 Dec 2025.
- Individual participant dataSharing planned: Yes. Data may be shared after the publication of the study
Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
An observational study of Evaluate the Fluorescence of Natural Tooth Following Dehydration, run by Marmara University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 1 inclusion and 1 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: under way, not looking for participants, as of the sponsor's last update on 22 Dec 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Digital photograph with Ashur plaque indicator (time frame: baseline). The study lists 9 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 22 Dec 2025; AzTrials last refreshed its copy on 6 Nov 2026.