ExerciseNCT07569627
Effects of BFR-training on Stem Cells and Immune Cells in Human Skeletal Muscle
A study testing BFR (180 mmHg), NMN-BFR exercise and Vitamin C-resistance exercise in people with Exercise and Blood Flow Restriction (BFR) Training Effects, run by University of Taipei at 1 site in Taiwan.
Trial summary
In this 3-year project, investigators will first measure stem cells (primary outcome) and immune cells (secondary outcome) in human skeletal muscle immediately after and 24 hour after the BFR-training. Read more about this study
- Who is it for?
- Ages 20 to 30 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Exercise, Blood Flow Restriction (BFR) Training Effects, Supplement
- What is being tested?
- BFR (180 mmHg), NMN-BFR exercise and Vitamin C-resistance exercise
- Type of treatment
- Lifestyle or therapy programme
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 60 participants
- Where
- 1 siteTaiwan
- Study dates
- 1 Aug 2022to 1 May 2025
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 20 to 30 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- healthy men
Who cannot join
- inflammatory or immune conditions
Trial locations and contacts
1 site in 1 country.
About this study
In this 3-year project, investigators will first measure stem cells (primary outcome) and immune cells (secondary outcome) in human skeletal muscle immediately after and 24 hour after the BFR-training. In the second and third year, the effect of β-NMN (vitamin B3 precursor) and antioxidant on the same outcomes after BFR-exercise will be assessed. The BFR-exercise in the study will consist of a 5 × 10-second sprinting with maximal voluntary efforts, 5 minutes after a BFR pre-conditioning to legs using pressurized cuffs. In Study 1 (1st year, N = 24, aged 20-30 year), a randomized crossover trial will be conducted to compare the efficacy of sprinting exercise using BFR pre-conditioning at 20 mmHg (control) or 180 mmHg (occlusion). Study 2 (2nd year, N = 24, aged 20-30 years) will be conducted to examine the effect of β-NMN supplementation on the aforementioned outcomes in human muscles using a randomized placebo-controlled crossover study design. Study 3 (third year, N = 24, aged 20-30 years) will be conducted to examine the effect of vitamin C on the same variables immediately after and 24 hours after resistance exercise intervention, using a randomized placebo-controlled crossover study design.
Read the detailed description
Primary outcome: Stem cells (Stro-1+ cells) assessed by immunofluorescence staining.
Secondary outcome: Immune cells (MPO+ cells) assessed by immunofluorescence staining.
Study 1: Two-arm intervention (counterbalanced crossover design): Sham-exercise, BFR-exercise Study 2: Two-arm intervention (counterbalanced crossover design): Placebo-BFR exercise, NMN-BFR exercise Study 3: Two-arm intervention (counterbalanced crossover design): Placebo-BFR exercise, Vitamin C-BFR exercise
- Being tested
- BFR (180 mmHg), NMN-BFR exercise, Vitamin C-resistance exercise
- Keywords
- NMN, vitamin C, exercise, BFR
- Medical subject headings
- Motor Activity
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
BFR (180 mmHg)Behavioural
Also called Sham (20 mmHg)
Sprinting exercise after BFR
NMN-BFR exerciseDietary supplement
Also called Placebo-BFR exercise
NMN ingested before and after BFR exercise
Vitamin C-resistance exerciseDietary supplement
Also called Placebo-resistance exercise
Vitamin C ingested before and after resistance exercise
| Study group | Role | What they receive |
|---|---|---|
| Sham (20 mmHg) | Sham comparator | Study 1:
Sham: blood flow restriction (20 mmHg on leg) for 3-5 min before exercise Receives: Behavioral: BFR (180 mmHg) |
| BFR (180 mmHg) | Experimental | Study 1:
BFR: blood flow restriction (180 mmHg on leg) for 3-5 min before exercise Receives: Behavioral: BFR (180 mmHg) |
| Placebo-BFR exercise | Placebo comparator | Study 2:
Placebo (cornstarch)-ingestion before and after BFR-exercise Receives: Dietary Supplement: NMN-BFR exercise |
| NMN-BFR exercise | Experimental | Study 2:
NMN-ingestion before and after BFR-exercise Receives: Dietary Supplement: NMN-BFR exercise |
| Placebo-resistance exercise | Placebo comparator | Study 3:
Cornstarch-ingestion before and after resistance exercise Receives: Dietary Supplement: Vitamin C-resistance exercise |
| Vitamin C-resistance exercise | Experimental | Study 3:
Vitamin C-ingestion before and after BFR-exercise Receives: Dietary Supplement: Vitamin C-resistance exercise |
What is being measured
The primary outcome is the main question the study is designed to answer.
Stem cells in muscle
Stem cells are identified by Stro-1 cell marker
Measured: Pre-exercise, immediately post-exercise, and 24-hour post-challenge (BFR-exercise) recovery
Secondary outcomes (1)
Immune cells in muscle
Immune cells are identified by myeloperoxidase (MPO) cell marker
Measured: At 24-hour post-challenge recovery
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted6 May 2026
- Study start1 Aug 2022
- Primary completion1 May 2025
- Study completion1 May 2025
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for ExerciseStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Crossover
- Model description
- Three studies will be conducted: Study 1: 2-arm Study 2: 2-arm Study 3: 2-arm
- Masking
- Triple blind
- Who is masked
- Participant, Care provider, Outcomes assessor
- Enrolment
- 60 participants (actual)
Sponsor and investigators
- Lead sponsor
- University of Taipei
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Chia-Hua Kuo, University of Taipei
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07569627
- Sponsor's protocol ID
- IRB-2021-082
- Other IDs
- MOST 111-2410-H-845 -005 -MY3
- Status last verified
- Jul 2026
- First submitted
- 7 Apr 2026
- Last updated
- 13 Jul 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Study at a glance
From the registry record, last updated by the sponsor on 13 Jul 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Effects of BFR-training on Stem Cells and Immune Cells in Human Skeletal Muscle
- ClinicalTrials.gov ID
- NCT07569627
- Acronym / sponsor ID
- IRB-2021-082
- Keywords
- NMN, vitamin C, exercise, BFR
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 60 participants (actual)
- Lead sponsor
- University of TaipeiUniversity, hospital or other
- Study start
- 1 Aug 2022 (actual)
- Primary completion
- 1 May 2025 (actual)
- Study completion
- 1 May 2025 (actual)
- First posted
- 6 May 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
In this 3-year project, investigators will first measure stem cells (primary outcome) and immune cells (secondary outcome) in human skeletal muscle immediately after and 24 hour after the BFR-training. In the second and third year, the effect of β-NMN (vitamin B3 precursor) and antioxidant on the same outcomes after BFR-exercise will be assessed. The BFR-exercise in the study will consist of a 5 × 10-second sprinting with maximal voluntary efforts, 5 minutes after a BFR pre-conditioning to legs using pressurized cuffs. In Study 1 (1st year, N = 24, aged 20-30 year), a randomized crossover trial will be conducted to compare the efficacy of sprinting exercise using BFR pre-conditioning at 20 mmHg (control) or 180 mmHg (occlusion). Study 2 (2nd year, N = 24, aged 20-30 years) will be conducted to examine the effect of β-NMN supplementation on the aforementioned outcomes in human muscles using a randomized placebo-controlled crossover study design. Study 3 (third year, N = 24, aged 20-30 years) will be conducted to examine the effect of vitamin C on the same variables immediately after and 24 hours after resistance exercise intervention, using a randomized placebo-controlled crossover study design.
Detailed description
Primary outcome: Stem cells (Stro-1+ cells) assessed by immunofluorescence staining.
Secondary outcome: Immune cells (MPO+ cells) assessed by immunofluorescence staining.
Study 1: Two-arm intervention (counterbalanced crossover design): Sham-exercise, BFR-exercise Study 2: Two-arm intervention (counterbalanced crossover design): Placebo-BFR exercise, NMN-BFR exercise Study 3: Two-arm intervention (counterbalanced crossover design): Placebo-BFR exercise, Vitamin C-BFR exercise
Study design
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Triple blind (participant, care provider, outcomes assessor)
- Number of arms
- 6
- Model description
- Three studies will be conducted: Study 1: 2-arm Study 2: 2-arm Study 3: 2-arm
| Arm | Type | Receives |
|---|---|---|
| Sham (20 mmHg) | Sham comparator | Behavioral: BFR (180 mmHg)Study 1: Sham: blood flow restriction (20 mmHg on leg) for 3-5 min before exercise |
| BFR (180 mmHg) | Experimental | Behavioral: BFR (180 mmHg)Study 1: BFR: blood flow restriction (180 mmHg on leg) for 3-5 min before exercise |
| Placebo-BFR exercise | Placebo comparator | Dietary Supplement: NMN-BFR exerciseStudy 2: Placebo (cornstarch)-ingestion before and after BFR-exercise |
| NMN-BFR exercise | Experimental | Dietary Supplement: NMN-BFR exerciseStudy 2: NMN-ingestion before and after BFR-exercise |
| Placebo-resistance exercise | Placebo comparator | Dietary Supplement: Vitamin C-resistance exerciseStudy 3: Cornstarch-ingestion before and after resistance exercise |
| Vitamin C-resistance exercise | Experimental | Dietary Supplement: Vitamin C-resistance exerciseStudy 3: Vitamin C-ingestion before and after BFR-exercise |
Who may qualify
- Ages
- 20 to 30 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (1 listed)
- healthy men
Key exclusion criteria (1 listed)
- inflammatory or immune conditions
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| BFR (180 mmHg)Also: Sham (20 mmHg)Sprinting exercise after BFR | Behavioural | BFR (180 mmHg), Sham (20 mmHg) |
| NMN-BFR exerciseAlso: Placebo-BFR exerciseNMN ingested before and after BFR exercise | Dietary supplement | NMN-BFR exercise, Placebo-BFR exercise |
| Vitamin C-resistance exerciseAlso: Placebo-resistance exerciseVitamin C ingested before and after resistance exercise | Dietary supplement | Placebo-resistance exercise, Vitamin C-resistance exercise |
Primary outcome
Stem cells in muscle
Time frame: Pre-exercise, immediately post-exercise, and 24-hour post-challenge (BFR-exercise) recovery
Stem cells are identified by Stro-1 cell marker
1 secondary outcome
Immune cells in muscle
Time frame: At 24-hour post-challenge recovery
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 13 Jul 2026.
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing BFR (180 mmHg), NMN-BFR exercise and Vitamin C-resistance exercise in people with Exercise and Blood Flow Restriction (BFR) Training Effects, run by University of Taipei at 1 site in Taiwan.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 1 inclusion and 1 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 13 Jul 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Taiwan. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Stem cells in muscle (time frame: Pre-exercise, immediately post-exercise, and 24-hour post-challenge (BFR-exercise) recovery). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 13 Jul 2026.