Muscle Strength DevelopmentAcute EffectsNCT07589712
Acute Effects of Proprioceptive Neuromuscular Facilitation on Hamstring-to-Quadriceps Strength Ratio
A study testing PNF Stretching and Static Stretching in people with Muscle Strength Development and Muscle Strength, run by Gümüşhane Universıty at 1 site in Turkey (Türkiye).
Trial summary
This study aimed to compare the acute effects of proprioceptive neuromuscular facilitation (PNF) and static stretching on hamstring-to-quadriceps (H/Q) strength ratios in healthy young men. A randomized crossover design was used in which participants completed both stretching protocols on separate days. Read more about this study
- Who is it for?
- Ages 18 to 25 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Muscle Strength Development, Muscle Strength, Neuromuscular Function
- What is being tested?
- PNF Stretching and Static Stretching
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 23 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 5 Mar 2025to 20 Apr 2025
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 25 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Age between 18 - 25 years, healthy male individuals
- Physically active (≥3 days/week, ≥30 min/day)
Who cannot join
- Musculoskeletal injury in the last 6 months
- Any neurological or orthopedic disorder
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Gumushane Univetsity
Gümüşhane, Trabzon, Ordu, Giresun, Rize, Artvin, Gümüşhane, 29600
About this study
Official title: Acute Effects of Proprioceptive Neuromuscular Facilitation and Static Stretching on Hamstring-to-Quadriceps Strength Ratio in Healthy Young Men
This study aimed to compare the acute effects of proprioceptive neuromuscular facilitation (PNF) and static stretching on hamstring-to-quadriceps (H/Q) strength ratios in healthy young men. A randomized crossover design was used in which participants completed both stretching protocols on separate days. Isokinetic muscle strength and H/Q ratios were assessed following each intervention. The findings provide insight into the immediate effects of different stretching modalities on muscle balance and performance.
Read the detailed description
The present study investigated the acute effects of two commonly used stretching modalities-proprioceptive neuromuscular facilitation (PNF) and static stretching-on isokinetic muscle strength and hamstring-to-quadriceps (H/Q) ratios in physically active healthy young males. The study employed a randomized crossover design with repeated measures, allowing each participant to complete both experimental conditions under standardized laboratory settings.
Participants attended three sessions: a familiarization session followed by two experimental sessions separated by at least 48 hours. Each experimental session included a standardized warm-up, followed by either PNF or static stretching intervention, and subsequent isokinetic strength testing. Peak torque values for quadriceps (concentric) and hamstrings (concentric and eccentric) were measured using an isokinetic dynamometer at 60°/s. Traditional and functional H/Q ratios were calculated.
The primary aim was to determine whether these stretching modalities produce acute alterations in muscle strength and H/Q ratios. The study also examined whether the type of ratio (traditional vs. functional) significantly influences outcomes. Findings contribute to understanding the neuromuscular implications of stretching in warm-up protocols.
- Being tested
- PNF Stretching, Static Stretching
- Keywords
- PNF stretching, Static stretching, Hamstring quadriceps ratio, Isokinetic strength, Acute effect
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
PNF StretchingOther
Participants performed four repetitions of PNF stretching consisting of a 6-second isometric hamstring contraction at approximately 60% of maximal voluntary contraction followed by a 30-second passive stretch. Rest intervals were standardized.
Static StretchingOther
Participants performed four repetitions of static hamstring stretching, each lasting 30 seconds, with 30-second rest intervals between repetitions.
| Study group | Role | What they receive |
|---|---|---|
| PNF Stretching | Experimental | Participants performed four repetitions of PNF stretching consisting of a 6-second isometric hamstring contraction at approximately 60% of maximal voluntary contraction followed by a 30-second passive stretch. Rest intervals were standardized. Receives: Other: PNF Stretching; Other: Static Stretching |
| Static Stretching | Experimental | Participants performed four repetitions of static hamstring stretching, each lasting 30 seconds, with 30-second rest intervals between repetitions Receives: Other: PNF Stretching; Other: Static Stretching |
What is being measured
The primary outcome is the main question the study is designed to answer.
Hamstring-to-Quadriceps (H/Q) Ratio
Both traditional (Hcon/Qcon) and functional (Hecc/Qcon) ratios were calculated using isokinetic peak torque values.
Measured: Immediately after intervention
Secondary outcomes (3)
Quadriceps Concentric Peak Torque
Quadriceps Concentric Peak Torque
Measured: Immediately post-intervention
Hamstring Concentric Peak Torque
Hamstring Concentric Peak Torque
Measured: Immediately post-intervention
Hamstring Eccentric Peak Torque
Hamstring Eccentric Peak Torque
Measured: Immediately post-intervention
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted15 May 2026
- Study start5 Mar 2025
- Primary completion15 Apr 2025
- Study completion20 Apr 2025
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Muscle Strength DevelopmentStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Model description
- Crossover Assignment
- Masking
- None (open label)
- Enrolment
- 23 participants (actual)
Sponsor and investigators
- Lead sponsor
- Gümüşhane Universıty
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Coşkun YILMAZ, Gümüşhane Universıty
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07589712
- Sponsor's protocol ID
- 2025/169
- Acronym
- Acute Effects
- Status last verified
- May 2026
- First submitted
- 6 May 2026
- Last updated
- 15 May 2026
- Results posted
- No
- Data monitoring committee
- Yes
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No: Individual participant data will not be shared.
Study at a glance
From the registry record, last updated by the sponsor on 15 May 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Acute Effects of Proprioceptive Neuromuscular Facilitation and Static Stretching on Hamstring-to-Quadriceps Strength Ratio in Healthy Young Men
- ClinicalTrials.gov ID
- NCT07589712
- Acronym / sponsor ID
- Acute Effects / 2025/169
- Keywords
- PNF stretching, Static stretching, Hamstring quadriceps ratio, Isokinetic strength, Acute effect
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 23 participants (actual)
- Lead sponsor
- Gümüşhane UniversıtyUniversity, hospital or other
- Study start
- 5 Mar 2025 (actual)
- Primary completion
- 15 Apr 2025 (actual)
- Study completion
- 20 Apr 2025 (actual)
- First posted
- 15 May 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This study aimed to compare the acute effects of proprioceptive neuromuscular facilitation (PNF) and static stretching on hamstring-to-quadriceps (H/Q) strength ratios in healthy young men. A randomized crossover design was used in which participants completed both stretching protocols on separate days. Isokinetic muscle strength and H/Q ratios were assessed following each intervention. The findings provide insight into the immediate effects of different stretching modalities on muscle balance and performance.
Detailed description
The present study investigated the acute effects of two commonly used stretching modalities-proprioceptive neuromuscular facilitation (PNF) and static stretching-on isokinetic muscle strength and hamstring-to-quadriceps (H/Q) ratios in physically active healthy young males. The study employed a randomized crossover design with repeated measures, allowing each participant to complete both experimental conditions under standardized laboratory settings.
Participants attended three sessions: a familiarization session followed by two experimental sessions separated by at least 48 hours. Each experimental session included a standardized warm-up, followed by either PNF or static stretching intervention, and subsequent isokinetic strength testing. Peak torque values for quadriceps (concentric) and hamstrings (concentric and eccentric) were measured using an isokinetic dynamometer at 60°/s. Traditional and functional H/Q ratios were calculated.
The primary aim was to determine whether these stretching modalities produce acute alterations in muscle strength and H/Q ratios. The study also examined whether the type of ratio (traditional vs. functional) significantly influences outcomes. Findings contribute to understanding the neuromuscular implications of stretching in warm-up protocols.
Study design
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- None (open label)
- Number of arms
- 2
- Model description
- Crossover Assignment
| Arm | Type | Receives |
|---|---|---|
| PNF Stretching | Experimental | Other: PNF Stretching, Other: Static StretchingParticipants performed four repetitions of PNF stretching consisting of a 6-second isometric hamstring contraction at approximately 60% of maximal voluntary contraction followed by a 30-second passive stretch. Rest intervals were standardized. |
| Static Stretching | Experimental | Other: PNF Stretching, Other: Static StretchingParticipants performed four repetitions of static hamstring stretching, each lasting 30 seconds, with 30-second rest intervals between repetitions |
Who may qualify
- Ages
- 18 to 25 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (2 listed)
- Age between 18 - 25 years, healthy male individuals
- Physically active (≥3 days/week, ≥30 min/day)
Key exclusion criteria (2 listed)
- Musculoskeletal injury in the last 6 months
- Any neurological or orthopedic disorder
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| PNF StretchingParticipants performed four repetitions of PNF stretching consisting of a 6-second isometric hamstring contraction at approximately 60% of maximal voluntary contraction followed by a 30-second passive stretch. Rest intervals were standardized. | Other | PNF Stretching, Static Stretching |
| Static StretchingParticipants performed four repetitions of static hamstring stretching, each lasting 30 seconds, with 30-second rest intervals between repetitions. | Other | PNF Stretching, Static Stretching |
Primary outcome
Hamstring-to-Quadriceps (H/Q) Ratio
Time frame: Immediately after intervention
Both traditional (Hcon/Qcon) and functional (Hecc/Qcon) ratios were calculated using isokinetic peak torque values.
3 secondary outcomes
Quadriceps Concentric Peak Torque
Time frame: Immediately post-intervention
Hamstring Concentric Peak Torque
Time frame: Immediately post-intervention
Hamstring Eccentric Peak Torque
Time frame: Immediately post-intervention
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 15 May 2026.
- Individual participant dataSharing planned: No. Individual participant data will not be shared.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing PNF Stretching and Static Stretching in people with Muscle Strength Development and Muscle Strength, run by Gümüşhane Universıty at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 2 inclusion and 2 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 15 May 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Hamstring-to-Quadriceps (H/Q) Ratio (time frame: Immediately after intervention). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 15 May 2026.