Individualized AML Treatment

The investigators will utilize high throughput drug screening (HTS) to assess sensitivity of patient's leukemic cells to drugs and drug combinations (~135). Bone marrow aspirate (BMA) or peripheral blood blasts, or if these are not involved, then bone marrow or tissue biopsy or involved fluids will be used for testing in the HTS and time to HTS report will be recorded. Genomics analysis (sequencing) will be utilized to additionally inform drug choice using the City of Hope HopeSeq Heme Comprehensive (HopeSeq) targeted next generation sequencing (NGS) and RNA seq assays. The turnaround time (TAT) of the above NGS assays is 8-10 business days (≈12-14 calendar days). The Cancer Drug Sensitivity Test Diagnostic (CDST) is a HTS assay with a faster TAT (5-7 calendar days) that is CLIA-approved for patient diagnostic purposes. CDST HTS will be performed in the Quellos High Throughput Core Facility at the University of Washington (UW) in Seattle, WA. These assays detect relevant genomic, and transcriptomic, and functional data events which the investigators hypothesize will provide an opportunity to rationally tailor drug selections and combinations for the treatment of patients with AML that have relapsed and/or refractory AML. HopeSeq, which includes most of the relevant genomic pathways in hematologic malignancies, will be performed on peripheral blood (PB) or bone marrow aspirate (BMA), or if insufficient cells, formalin fixed paraffin embedded (FFPE) tissues may also be analyzed. HopeSeq will be performed as conventional care ~14 days prior to, or at the time of study enrollment, or study will access the results through the Hematopoietic Tissue Biorepository (HTB) at City of Hope. Based on the molecular diagnosis report from HopeSeq and the CDST HTS report, the investigators A propose to convene a Functional Molecular Tumor Board (FMTB) will meet that will consider the data from the molecular and functional studies, as well as information from a literature review for clinical and laboratory findings for other patients with the specific clinical features and molecular/functional data that will offer patients and their physicians treatment recommendations possible treatment options based on multiomic data (HopeSeq and CDST HTS reports) as well as clinical features within 14-18 days from sample acquisition.

But ultimately, the patient's treating physician will decide which regimen is best considering individual patient factors such as performance status, prior regimens, insurance authorization, and patient preferences. The retrospective correlation with patient response will enable determination of the relative predictive contribution of each of the tests, both positive and negative predictive value.