HealthyNCT07641907
An Initial Investigation Into the Effects of β-Alanine on Performance and Physiological Responses During Simulated Wrestling Matches
A study testing Beta-Alanine (Beta-Alanine trial) in people with Healthy and Athletes, run by China Medical University Hospital at 1 site in Taiwan.
Trial summary
This research aims to investigate the effects of beta-alanine (BA) supplementation on simulated 2-day freestyle wrestling performance, as well as on exercise-induced muscle fatigue and oxidative stress in wrestlers. Read more about this study
- Who is it for?
- Ages 12 to 25 yearsHealthy volunteers can take part
- What is being tested?
- Beta-Alanine (Beta-Alanine trial)
- Type of treatment
- Pill or oral medicine
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 16 participants
- Where
- 1 siteTaiwan
- Study dates
- 11 Apr 2026to 1 Oct 2026
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 12 to 25 years
- All sexes
- Healthy volunteers accepted
- Child, Adult
Who may be able to join
- Wrestle athletes aged 12 to 25 years with at least 3 years of wrestle training experience.
Who cannot join
- All participants must be free from chronic conditions such as heart disease and hypertension, and must not have experienced any serious lower limb musculoskeletal injuries within the past six months.
Trial locations and contacts
1 site in 1 country.
About this study
This research aims to investigate the effects of beta-alanine (BA) supplementation on simulated 2-day freestyle wrestling performance, as well as on exercise-induced muscle fatigue and oxidative stress in wrestlers. In addition to the common issue of long-term insufficient energy intake due to weight control, high-intensity training can further exacerbate exercise-induced oxidative stress and muscle fatigue, thereby impairing athletic performance. Therefore, finding effective ergogenic aids to reduce these negative physiological responses is crucial for optimizing athletes' competitive condition. Based on a literature review, this study will implement the Specific Wrestling Performance Test (SWPT) to simulate two consecutive days of freestyle wrestling competitions according to Kraemer's model. Multiple performance assessments will be used to evaluate the impact of BA supplementation on athletes' physiological indicators and performance. It is expected that BA supplementation will enhance systemic antioxidant capacity, reduce muscle fatigue and oxidative stress induced by simulated wrestling matches, and improve wrestling-specific physical performance.
Read the detailed description
This experiment will employ the Specific Wrestling Performance Test (SWPT) to conduct the two-day simulated freestyle wrestling competition protocol developed by Kraemer (Kraemer et al., 2001), in order to evaluate the physiological responses of athletes during competition. A total of 20 wrestlers will participate in this study using a randomized single-blind crossover design. Participants will receive either beta-alanine (BA) supplementation or a placebo for four weeks (4.8 g/day), followed by a four-week washout period. On the official testing day, body composition measurements and records will be taken 12 hours before the first SWPT session. After the measurements, participants will be provided with a 300 kcal light meal. Following the completion of the third SWPT session, participants will perform approximately 20-30 minutes of cycling exercise to help maintain body weight for the following morning. Two hours before the fourth SWPT session, body weight will be measured again to ensure that weight variation does not exceed 2%, after which another 300 kcal light meal will be provided. The simulated two-day freestyle wrestling competition protocol will consist of five SWPT sessions: on Day 1, SWPT sessions will be conducted at 10:00 AM, 2:00 PM, and 6:00 PM; on Day 2, sessions will take place at 10:00 AM and 7:00 PM. In this crossover trial, all pre-competition assessments will be conducted before the matches. Pre-test assessments must be completed 10 minutes before the start of the first SWPT session, while post-test assessments will be conducted immediately after each SWPT session. The assessment items include the number of throws completed during the simulated SWPT match, reaction tests, grip strength tests, back strength tests, and sport-specific fitness tests. In addition, ratings of perceived exertion (RPE), heart rate, and blood samples will be recorded and collected. The sport-specific fitness tests include the Overhead Medicine Ball Throw (OMBT) and the Medicine Ball Chest Throw (MBCT).
- Being tested
- Placebo (Placebo trial), Beta-Alanine (Beta-Alanine trial)
- Keywords
- beta-alanine, wrestle, exercise performance
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Placebo (Placebo trial)Other
The Placebo capsule supplement was taken for 4 weeks (6 capsules per day).
Beta-Alanine (Beta-Alanine trial)Other
The Beta-Alanine capsule supplement was taken for 4 weeks, with a daily dosage of 4.8 g of Beta-Alanine (equivalent to 6 capsules per day, each containing 800 mg of Beta-Alanine).
| Study group | Role | What they receive |
|---|---|---|
| Placebo | Placebo comparator | Containing edible yellow No. 4, edible yellow No. 5, sucrose, silica, talc, oxidized starch, gelatin, magnesium stearate, and palm wax. Receives: Other: Placebo (Placebo trial); Other: Beta-Alanine (Beta-Alanine trial) |
| Beta-Alanine | Experimental | Each capsule containing 800 mg of Beta-Alanine. Receives: Other: Placebo (Placebo trial); Other: Beta-Alanine (Beta-Alanine trial) |
What is being measured
The primary outcome is the main question the study is designed to answer.
Specific Wrestling Performance Test (SWPT)
The test consists of two rounds with identical procedures. Each round lasts for 3 minutes, with a 30-second rest interval between rounds. During the first minute, participants perform one throw every 10 seconds for a total of 3 throws. This is followed by maximal repeated throws performed between 30 and 50 seconds, and then a rest period from 50 to 60 seconds. The second minute follows the same procedure as the first minute. In the third minute, participants again perform one throw every 10 seconds for a total of 3 throws, followed by 30 seconds of maximal repeated throws, during which the total number of throws is recorded. According to the experimental design of Marković, the dummy weight is adjusted based on the athlete's body weight: participants weighing less than 75 kg throw a 22 kg dummy, those weighing between 75-90 kg throw a 27 kg dummy, and those weighing more than 90 kg throw a 32 kg dummy (Marković et al., 2022).
Measured: Including for 4 days in both beta-alanine and placebo trials
Secondary outcomes (6)
Reaction test
Reaction time is a key factor influencing wrestling performance, as shorter reaction times enable athletes to execute more effective technical and tactical actions. In this study, the BlazePod reaction light system will be used to conduct the reaction test. After hearing the command "Ready," participants will assume a position with both hands and knees on the ground. Once the light is activated, participants must stand up as quickly as possible and tap the reaction light. The time interval between the "Ready" command and the light activation will vary randomly in order to prevent participants from anticipating the timing of the stimulus. The test will be performed three times with a 1-minute rest interval between trials, and the best performance result will be recorded.
Measured: Including for 4 days in both beta-alanine and placebo trials
Grip strength test
Grip strength will be assessed using an electronic handgrip dynamometer. Participants will stand in a natural upright position with their arms hanging naturally at their sides. The elbows and wrists will be maintained at 0° extension, and the grip width will be adjusted to fit the position of the second finger joint. During the test, participants will be instructed to squeeze the dynamometer handle as forcefully as possible for 5 seconds. Grip strength measurements will be performed three times for each hand, with a 1-minute rest interval between trials. The highest value obtained will be recorded as the test result.
Measured: Including for 4 days in both beta-alanine and placebo trials
The Borg Rating of Perceived Exertion (RPE) Scale
The Borg Rating of Perceived Exertion Scale measures how hard exercise feels. Participants rate effort from 6 (no exertion) to 20 (maximal exertion). A higher score means greater effort (worse fatigue).
Measured: Including for 4 days in both beta-alanine and placebo trials
Medicine Ball Chest Throw (MBCT) test
The sport-specific wrestling fitness test protocol developed by Dehnou in 2020 will be used in this study. Participants will sit on a chair with their feet shoulder-width apart and firmly placed on the floor, with the toes aligned with the starting line. The knees will be maintained at a 90° flexion angle while the upper body remains upright. Participants will hold a 5 kg medicine ball in front of the chest with both hands. During the test, participants will forcefully push the ball forward using a chest-pass motion similar to a basketball chest pass. Throughout the movement, the ischial tuberosities must remain in contact with the seat, and trunk rotation is not permitted. The throwing distance will be measured and recorded. The test will be performed three times with a 1-minute rest interval between trials, and the greatest distance achieved will be recorded as the final result.
Measured: Including for 4 days in both beta-alanine and placebo trials
Overhead Medicine Ball Throw (OMBT) test
The sport-specific wrestling fitness test protocol developed by Dehnou in 2020 will be adopted in this study. Participants will stand with their feet shoulder-width apart and their heels aligned with the starting line, while holding a 5 kg medicine ball naturally in front of the body with both hands. Participants will then squat down until the medicine ball reaches knee level, after which they will rapidly extend the body and forcefully throw the ball backward over the head. The throwing distance will be measured and recorded. The test will be performed three times with a 1-minute rest interval between trials, and the greatest distance achieved will be recorded as the final result.
Measured: Including for 4 days in both beta-alanine and placebo trials
Back strength test
Back strength will be measured using a back strength dynamometer. Participants will stand on the testing platform while gripping the handle with both hands using an overhand grip. The knees will remain fully extended, and the upper body will lean forward at approximately 30°. The length of the chain attached to the handle may be adjusted according to each participant's body size to ensure an appropriate testing position. During the test, participants will pull the handle upward with maximal effort using the strength of the back and upper body muscles, while avoiding excessive use of the arms and legs. The dynamometer pointer will display the measured back strength value. The test will be performed three times with a 1-minute rest interval between trials, and the highest value obtained will be recorded.
Measured: Including for 4 days in both beta-alanine and placebo trials
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted11 Jun 2026
- Study start11 Apr 2026
- Primary completion24 May 2026
- Study completion1 Oct 2026 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for HealthyStudies of the same condition, open and under way.
- More trials run by China Medical University HospitalThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Model description
- This study plans to recruit 20 healthy male and female participants aged 12-25 years who have at least three years of wrestling training experience (training at least three times per week for a minimum of three years). All participants must be free from chronic diseases such as heart disease and hypertension, and must not have experienced any severe lower-extremity skeletal or muscular injuries within the past six months. Throughout the entire experimental period, participants will be required to maintain their normal lifestyle habits and refrain from consuming any other nutritional supplements, alcohol, or medications. Students currently taught or advised by the principal investigator or members of the research team, as well as individuals who have consulting or supervisory relationships with them, will be excluded from participation in this study.
- Masking
- Quadruple blind
- Who is masked
- Participant, Care provider, Investigator, Outcomes assessor
- Enrolment
- 16 participants (actual)
Sponsor and investigators
- Lead sponsor
- China Medical University Hospital
- Sponsor type
- University, hospital or other
- Collaborators
- China Medical University, Taiwan
- Responsible party
- Sponsor
- Investigators
- Jung-Piao Tsao, Study chair, China Medical University, China
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07641907
- Sponsor's protocol ID
- CMUH114-REC3-170
- Status last verified
- Jun 2026
- First submitted
- 8 Jun 2026
- Last updated
- 11 Jun 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Study at a glance
From the registry record, last updated by the sponsor on 11 Jun 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- An Initial Investigation Into the Effects of β-Alanine on Performance and Physiological Responses During Simulated Wrestling Matches
- ClinicalTrials.gov ID
- NCT07641907
- Acronym / sponsor ID
- CMUH114-REC3-170
- Keywords
- beta-alanine, wrestle, exercise performance
- Study type
- Interventional
- Recruitment status
- Active, not recruiting
- Enrolment
- 16 participants (actual)
- Lead sponsor
- China Medical University HospitalUniversity, hospital or other
- Collaborators
- China Medical University, Taiwan
- Study start
- 11 Apr 2026 (actual)
- Primary completion
- 24 May 2026 (actual)
- Study completion
- 1 Oct 2026 (estimated)
- First posted
- 11 Jun 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This research aims to investigate the effects of beta-alanine (BA) supplementation on simulated 2-day freestyle wrestling performance, as well as on exercise-induced muscle fatigue and oxidative stress in wrestlers. In addition to the common issue of long-term insufficient energy intake due to weight control, high-intensity training can further exacerbate exercise-induced oxidative stress and muscle fatigue, thereby impairing athletic performance. Therefore, finding effective ergogenic aids to reduce these negative physiological responses is crucial for optimizing athletes' competitive condition. Based on a literature review, this study will implement the Specific Wrestling Performance Test (SWPT) to simulate two consecutive days of freestyle wrestling competitions according to Kraemer's model. Multiple performance assessments will be used to evaluate the impact of BA supplementation on athletes' physiological indicators and performance. It is expected that BA supplementation will enhance systemic antioxidant capacity, reduce muscle fatigue and oxidative stress induced by simulated wrestling matches, and improve wrestling-specific physical performance.
Detailed description
This experiment will employ the Specific Wrestling Performance Test (SWPT) to conduct the two-day simulated freestyle wrestling competition protocol developed by Kraemer (Kraemer et al., 2001), in order to evaluate the physiological responses of athletes during competition. A total of 20 wrestlers will participate in this study using a randomized single-blind crossover design. Participants will receive either beta-alanine (BA) supplementation or a placebo for four weeks (4.8 g/day), followed by a four-week washout period. On the official testing day, body composition measurements and records will be taken 12 hours before the first SWPT session. After the measurements, participants will be provided with a 300 kcal light meal. Following the completion of the third SWPT session, participants will perform approximately 20-30 minutes of cycling exercise to help maintain body weight for the following morning. Two hours before the fourth SWPT session, body weight will be measured again to ensure that weight variation does not exceed 2%, after which another 300 kcal light meal will be provided. The simulated two-day freestyle wrestling competition protocol will consist of five SWPT sessions: on Day 1, SWPT sessions will be conducted at 10:00 AM, 2:00 PM, and 6:00 PM; on Day 2, sessions will take place at 10:00 AM and 7:00 PM. In this crossover trial, all pre-competition assessments will be conducted before the matches. Pre-test assessments must be completed 10 minutes before the start of the first SWPT session, while post-test assessments will be conducted immediately after each SWPT session. The assessment items include the number of throws completed during the simulated SWPT match, reaction tests, grip strength tests, back strength tests, and sport-specific fitness tests. In addition, ratings of perceived exertion (RPE), heart rate, and blood samples will be recorded and collected. The sport-specific fitness tests include the Overhead Medicine Ball Throw (OMBT) and the Medicine Ball Chest Throw (MBCT).
Study design
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Quadruple blind (participant, care provider, investigator, outcomes assessor)
- Number of arms
- 2
- Model description
- This study plans to recruit 20 healthy male and female participants aged 12-25 years who have at least three years of wrestling training experience (training at least three times per week for a minimum of three years). All participants must be free from chronic diseases such as heart disease and hypertension, and must not have experienced any severe lower-extremity skeletal or muscular injuries within the past six months. Throughout the entire experimental period, participants will be required to maintain their normal lifestyle habits and refrain from consuming any other nutritional supplements, alcohol, or medications. Students currently taught or advised by the principal investigator or members of the research team, as well as individuals who have consulting or supervisory relationships with them, will be excluded from participation in this study.
| Arm | Type | Receives |
|---|---|---|
| Placebo | Placebo comparator | Other: Placebo (Placebo trial), Other: Beta-Alanine (Beta-Alanine trial)Containing edible yellow No. 4, edible yellow No. 5, sucrose, silica, talc, oxidized starch, gelatin, magnesium stearate, and palm wax. |
| Beta-Alanine | Experimental | Other: Placebo (Placebo trial), Other: Beta-Alanine (Beta-Alanine trial)Each capsule containing 800 mg of Beta-Alanine. |
Who may qualify
- Ages
- 12 to 25 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Child, Adult
Key inclusion criteria (1 listed)
- Wrestle athletes aged 12 to 25 years with at least 3 years of wrestle training experience.
Key exclusion criteria (1 listed)
- All participants must be free from chronic conditions such as heart disease and hypertension, and must not have experienced any serious lower limb musculoskeletal injuries within the past six months.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Placebo (Placebo trial)The Placebo capsule supplement was taken for 4 weeks (6 capsules per day). | Other | Beta-Alanine, Placebo |
| Beta-Alanine (Beta-Alanine trial)The Beta-Alanine capsule supplement was taken for 4 weeks, with a daily dosage of 4.8 g of Beta-Alanine (equivalent to 6 capsules per day, each containing 800 mg of Beta-Alanine). | Other | Beta-Alanine, Placebo |
Primary outcome
Specific Wrestling Performance Test (SWPT)
Time frame: Including for 4 days in both beta-alanine and placebo trials
The test consists of two rounds with identical procedures. Each round lasts for 3 minutes, with a 30-second rest interval between rounds. During the first minute, participants perform one throw every 10 seconds for a total of 3 throws. This is followed by maximal repeated throws performed between 30 and 50 seconds, and then a rest period from 50 to 60 seconds. The second minute follows the same procedure as the first minute. In the third minute, participants again perform one throw every 10 seconds for a total of 3 throws, followed by 30 seconds of maximal repeated throws, during which the total number of throws is recorded. According to the experimental design of Marković, the dummy weight is adjusted based on the athlete's body weight: participants weighing less than 75 kg throw a 22 kg dummy, those weighing between 75-90 kg throw a 27 kg dummy, and those weighing more than 90 kg throw a 32 kg dummy (Marković et al., 2022).
6 secondary outcomes
Reaction test
Time frame: Including for 4 days in both beta-alanine and placebo trials
Grip strength test
Time frame: Including for 4 days in both beta-alanine and placebo trials
The Borg Rating of Perceived Exertion (RPE) Scale
Time frame: Including for 4 days in both beta-alanine and placebo trials
Medicine Ball Chest Throw (MBCT) test
Time frame: Including for 4 days in both beta-alanine and placebo trials
Overhead Medicine Ball Throw (OMBT) test
Time frame: Including for 4 days in both beta-alanine and placebo trials
Back strength test
Time frame: Including for 4 days in both beta-alanine and placebo trials
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 11 Jun 2026.
- Individual participant dataSharing planned: No
Related trials for Healthy
Recruiting
THE EFFECTS OF GAME-BASED EXERCISE PROGRAMS
Physical Activity, Body Image
Not yet recruitingPhase 1
A Phase I Study of GLR2053 Tablets in Chinese Healthy Adult Participants
Atopic Dermatitis
Recruiting
Colon-delivered Riboflavin and Gut Microbiota Composition
Microbiome Dysbiosis, Gut Health
Not yet recruiting
Neuroergonomic Analysis of a Brain-Computer Interface Device (BCI)
Healthy Volunteers - Male and Female
RecruitingPhase 1
First-in-Human Trial to Assess REGN20423 in Healthy Adult Participants and Adult Participants With Atopic Dermatitis
Healthy Volunteer, Atopic Dermatitis
Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Jung-Piao Tsao study chair
China Medical University, China
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Beta-Alanine (Beta-Alanine trial) in people with Healthy and Athletes, run by China Medical University Hospital at 1 site in Taiwan.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 1 inclusion and 1 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: under way, not looking for participants, as of the sponsor's last update on 11 Jun 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Taiwan. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Specific Wrestling Performance Test (SWPT) (time frame: Including for 4 days in both beta-alanine and placebo trials). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 11 Jun 2026.