Electromyographic Response to Blood Flow Restriction Exercise in Healthy AdultsNCT07654608
Electromyographic Response to Blood Flow Restriction Exercise in Healthy Adults
A study testing Bilateral low-load knee extension exercise protocol and Pneumatic blood flow restriction cuff in people with Electromyographic Response to Blood Flow Restriction Exercise in Healthy Adults, run by Universidad de Extremadura at 1 site in Spain.
Trial summary
This pilot randomized contralateral within-participant study evaluated the immediate neuromuscular response to low-load knee extension exercise performed with and without blood flow restriction in healthy young adults. Read more about this study
- Who is it for?
- Ages 18 to 65 yearsHealthy volunteers can take part
- Conditions the study is for
- Electromyographic Response to Blood Flow Restriction Exercise in Healthy Adults
- What is being tested?
- Bilateral low-load knee extension exercise protocol and Pneumatic blood flow restriction cuff
- Type of treatment
- Medical device, Surgery or procedure
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 24 participants
- Where
- 1 siteSpain
- Study dates
- 3 Mar 2026to 15 Apr 2026
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 65 years
- All sexes
- Healthy volunteers accepted
- Adult, Older adult
Who may be able to join
- Age between 18 and 65 years.
- Healthy adults.
- Absence of active pain or musculoskeletal injury in the lower limbs at the time of assessment.
- Ability to understand the study procedures.
- Voluntary agreement to participate and provision of written informed consent.
Who cannot join
- History of neuromuscular disease.
- History of cardiovascular disease.
- History of peripheral vascular disease.
- History of thromboembolic events.
- Uncontrolled hypertension.
- Skin lesions or skin conditions preventing placement of the blood flow restriction cuff or surface electromyography electrodes.
Show all 9Show fewer
- Recent lower limb surgery.
- Pregnancy.
- Any other contraindication to blood flow restriction exercise.
Trial locations and contacts
1 site in 1 country.
About this study
Official title: Electromyographic Analysis of Immediate Neuromuscular Response After Low-Load Knee Extension Exercise With and Without Blood Flow Restriction in Healthy Adults
This pilot randomized contralateral within-participant study evaluated the immediate neuromuscular response to low-load knee extension exercise performed with and without blood flow restriction in healthy young adults.
Participants were randomized in a 1:1 ratio to one of two limb-allocation groups: blood flow restriction applied to the dominant limb or blood flow restriction applied to the non-dominant limb. In each participant, the contralateral limb completed the same exercise protocol without blood flow restriction. Therefore, all participants were assessed under both experimental conditions.
Read more
Both limb-specific conditions were completed during the same experimental session. The order of the blood flow restriction and non-blood flow restriction conditions was randomized for each participant, with a 10-minute rest period between conditions.
Blood flow restriction was applied using a pneumatic cuff positioned on the proximal thigh. Cuff pressure was individualized and set at 80% of the arterial occlusion pressure measured for the corresponding limb. Participants completed the same standardized low-load knee extension protocol under both conditions.
Surface electromyography was used to record vastus lateralis activity from each assessed limb during the exercise protocol. Muscle activity was primarily evaluated using root mean square amplitude normalized to the pre-exercise maximal voluntary isometric contraction recorded from the corresponding limb. Root mean square amplitude during maximal voluntary isometric contraction trials was also recorded before and immediately after each condition.
The study aimed to compare the electromyographic response between the blood flow restriction and non-blood flow restriction conditions and to determine whether this response differed according to whether blood flow restriction was applied to the dominant or non-dominant limb.
- Being tested
- Bilateral low-load knee extension exercise protocol, Pneumatic blood flow restriction cuff
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Bilateral low-load knee extension exercise protocolProcedure or surgery
Each participant completed the low-load knee extension exercise protocol separately with both lower limbs. Under each condition, the protocol comprised 75 repetitions distributed across four sets of 30, 15, 15, and 15 repetitions, with 30 seconds of rest between sets. One limb performed the protocol with blood flow restriction and the contralateral limb performed it without blood flow restriction. Condition order was randomized, and a 10-minute rest period was provided between the two limb-specific conditions. Vastus lateralis surface electromyography was recorded continuously during each protocol.
Pneumatic blood flow restriction cuffDevice
A pneumatic cuff was placed on the proximal thigh of the limb randomized to the blood flow restriction condition and inflated to 80% of the individualized arterial occlusion pressure during the low-load knee extension protocol. The cuff was not applied to the contralateral limb, which completed the same protocol under the non-blood flow restriction condition. The cuff was fully deflated and removed after completion of the blood flow restriction condition.
| Study group | Role | What they receive |
|---|---|---|
| Experimental: BFR allocated to the dominant limb | Experimental | Participants received blood flow restriction on the dominant limb, while the contralateral non-dominant limb completed the same low-load knee extension protocol without blood flow restriction. Both limb-specific conditions were performed during the same experimental session in randomized order, with a 10-minute rest period between conditions. Blood flow restriction pressure was individualized and set at 80% of the arterial occlusion pressure of the corresponding limb. Vastus lateralis surface electromyography was recorded under both conditions. Receives: Procedure: Bilateral low-load knee extension exercise protocol; Device: Pneumatic blood flow restriction cuff |
| Experimental: BFR allocated to the non-dominant limb | Experimental | Participants received blood flow restriction on the non-dominant limb, while the contralateral dominant limb completed the same low-load knee extension protocol without blood flow restriction. Both limb-specific conditions were performed during the same experimental session in randomized order, with a 10-minute rest period between conditions. Blood flow restriction pressure was individualized and set at 80% of the arterial occlusion pressure of the corresponding limb. Vastus lateralis surface electromyography was recorded under both conditions. Receives: Procedure: Bilateral low-load knee extension exercise protocol; Device: Pneumatic blood flow restriction cuff |
What is being measured
The primary outcome is the main question the study is designed to answer.
Vastus Lateralis Electromyographic Amplitude Normalized to Maximal Voluntary Isometric Contraction
Surface electromyographic activity of the vastus lateralis was assessed during the low-load knee extension exercise protocol. The root mean square amplitude was normalized to the maximal voluntary isometric contraction recorded before exercise and expressed as percentage of maximal voluntary isometric contraction (%MVIC). Measurements were extracted at predefined time points across the four exercise sets in both experimental conditions.
Measured: During the exercise protocol, within a single experimental session.
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted17 Jun 2026
- Study start3 Mar 2026
- Primary completion15 Apr 2026
- Study completion15 Apr 2026
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Electromyographic Response to Blood Flow Restriction Exercise in Healthy AdultsStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Single blind
- Who is masked
- Outcomes assessor
- Enrolment
- 24 participants (actual)
Sponsor and investigators
- Lead sponsor
- Universidad de Extremadura
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Carlos Fernández-Morales, Universidad de Extremadura
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07654608
- Sponsor's protocol ID
- 13
- Status last verified
- Jul 2026
- First submitted
- 12 Jun 2026
- Last updated
- 27 Jul 2026
- Results posted
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Study at a glance
From the registry record, last updated by the sponsor on 27 Jul 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Electromyographic Analysis of Immediate Neuromuscular Response After Low-Load Knee Extension Exercise With and Without Blood Flow Restriction in Healthy Adults
- ClinicalTrials.gov ID
- NCT07654608
- Acronym / sponsor ID
- 13
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 24 participants (actual)
- Lead sponsor
- Universidad de ExtremaduraUniversity, hospital or other
- Study start
- 3 Mar 2026 (actual)
- Primary completion
- 15 Apr 2026 (actual)
- Study completion
- 15 Apr 2026 (actual)
- First posted
- 17 Jun 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This pilot randomized contralateral within-participant study evaluated the immediate neuromuscular response to low-load knee extension exercise performed with and without blood flow restriction in healthy young adults.
Participants were randomized in a 1:1 ratio to one of two limb-allocation groups: blood flow restriction applied to the dominant limb or blood flow restriction applied to the non-dominant limb. In each participant, the contralateral limb completed the same exercise protocol without blood flow restriction. Therefore, all participants were assessed under both experimental conditions.
Both limb-specific conditions were completed during the same experimental session. The order of the blood flow restriction and non-blood flow restriction conditions was randomized for each participant, with a 10-minute rest period between conditions.
Blood flow restriction was applied using a pneumatic cuff positioned on the proximal thigh. Cuff pressure was individualized and set at 80% of the arterial occlusion pressure measured for the corresponding limb. Participants completed the same standardized low-load knee extension protocol under both conditions.
Surface electromyography was used to record vastus lateralis activity from each assessed limb during the exercise protocol. Muscle activity was primarily evaluated using root mean square amplitude normalized to the pre-exercise maximal voluntary isometric contraction recorded from the corresponding limb. Root mean square amplitude during maximal voluntary isometric contraction trials was also recorded before and immediately after each condition.
The study aimed to compare the electromyographic response between the blood flow restriction and non-blood flow restriction conditions and to determine whether this response differed according to whether blood flow restriction was applied to the dominant or non-dominant limb.
Study design
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Single blind (outcomes assessor)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| Experimental: BFR allocated to the dominant limb | Experimental | Procedure: Bilateral low-load knee extension exercise protocol, Device: Pneumatic blood flow restriction cuffParticipants received blood flow restriction on the dominant limb, while the contralateral non-dominant limb completed the same low-load knee extension protocol without blood flow restriction. Both limb-specific conditions were performed during the same experimental session in randomized order, with a 10-minute rest period between conditions. Blood flow restriction pressure was individualized and set at 80% of the arterial occlusion pressure of the corresponding limb. Vastus lateralis surface electromyography was recorded under both conditions. |
| Experimental: BFR allocated to the non-dominant limb | Experimental | Procedure: Bilateral low-load knee extension exercise protocol, Device: Pneumatic blood flow restriction cuffParticipants received blood flow restriction on the non-dominant limb, while the contralateral dominant limb completed the same low-load knee extension protocol without blood flow restriction. Both limb-specific conditions were performed during the same experimental session in randomized order, with a 10-minute rest period between conditions. Blood flow restriction pressure was individualized and set at 80% of the arterial occlusion pressure of the corresponding limb. Vastus lateralis surface electromyography was recorded under both conditions. |
Who may qualify
- Ages
- 18 to 65 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult, Older adult
Key inclusion criteria (5 listed)
- Age between 18 and 65 years.
- Healthy adults.
- Absence of active pain or musculoskeletal injury in the lower limbs at the time of assessment.
- Ability to understand the study procedures.
- Voluntary agreement to participate and provision of written informed consent.
Key exclusion criteria (9 listed)
- History of neuromuscular disease.
- History of cardiovascular disease.
- History of peripheral vascular disease.
- History of thromboembolic events.
- Uncontrolled hypertension.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Bilateral low-load knee extension exercise protocolEach participant completed the low-load knee extension exercise protocol separately with both lower limbs. Under each condition, the protocol comprised 75 repetitions distributed across four sets of 30, 15, 15, and 15 repetitions, with 30 seconds of rest between sets. One limb performed the protocol with blood flow restriction and the contralateral limb performed it without blood flow restriction. Condition order was randomized, and a 10-minute rest period was provided between the two limb-specific conditions. Vastus lateralis surface electromyography was recorded continuously during each protocol. | Procedure or surgery | Experimental: BFR allocated to the dominant limb, Experimental: BFR allocated to the non-dominant limb |
| Pneumatic blood flow restriction cuffA pneumatic cuff was placed on the proximal thigh of the limb randomized to the blood flow restriction condition and inflated to 80% of the individualized arterial occlusion pressure during the low-load knee extension protocol. The cuff was not applied to the contralateral limb, which completed the same protocol under the non-blood flow restriction condition. The cuff was fully deflated and removed after completion of the blood flow restriction condition. | Device | Experimental: BFR allocated to the dominant limb, Experimental: BFR allocated to the non-dominant limb |
Primary outcome
Vastus Lateralis Electromyographic Amplitude Normalized to Maximal Voluntary Isometric Contraction
Time frame: During the exercise protocol, within a single experimental session.
Surface electromyographic activity of the vastus lateralis was assessed during the low-load knee extension exercise protocol. The root mean square amplitude was normalized to the maximal voluntary isometric contraction recorded before exercise and expressed as percentage of maximal voluntary isometric contraction (%MVIC). Measurements were extracted at predefined time points across the four exercise sets in both experimental conditions.
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 27 Jul 2026.
Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Bilateral low-load knee extension exercise protocol and Pneumatic blood flow restriction cuff in people with Electromyographic Response to Blood Flow Restriction Exercise in Healthy Adults, run by Universidad de Extremadura at 1 site in Spain.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 5 inclusion and 9 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 27 Jul 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Spain. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Vastus Lateralis Electromyographic Amplitude Normalized to Maximal Voluntary Isometric Contraction (time frame: During the exercise protocol, within a single experimental session.). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 27 Jul 2026.