PeriodontitisNCT07712627
Hypochlorous Acid in Non-Surgical Periodontal Therapy
A study testing Hypochlorous Acid Application and Scaling and Root Planing (SRP) with Purified Water in people with Periodontitis and Periodontitis Stage I, run by Ataturk University at 1 site in Turkey (Türkiye).
Trial summary
Periodontitis is a chronic inflammatory disease associated with dental plaque and characterized by the progressive destruction of the tooth-supporting tissues. The principal features of periodontitis include clinical attachment loss (CAL), alveolar bone loss, periodontal pocket formation, gingival bleeding, and the… Read more about this study
- Who is it for?
- Ages 18 to 65 years
- Conditions the study is for
- Periodontitis, Periodontitis Stage I, Periodontitis Stage II
- What is being tested?
- Hypochlorous Acid Application and Scaling and Root Planing (SRP) with Purified Water
- Type of treatment
- Surgery or procedure
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 44 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 9 Oct 2025to 12 Jun 2026
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 65 years
- All sexes
- No healthy volunteers
- Adult, Older adult
Who may be able to join
- Individuals aged 18 to 65 years.
- Systemically healthy individuals.
- Diagnosis of Stage I or Stage II periodontitis according to the 2017 Classification of Periodontal Diseases.
- Probing pocket depth (PPD) ≤5 mm and clinical attachment loss (CAL) ≤4 mm.
- Presence of a sufficient number of teeth for periodontal evaluation.
- Willingness to participate and ability to provide written informed consent.
Who cannot join
- Patients with contraindications to the use of ultrasonic instrumentation.
- Pregnant or lactating women.
- Patients receiving anti-inflammatory or antibiotic therapy.
- Patients with systemic diseases or medical conditions.
- Patients receiving continuous medication for any medical condition.
- Patients who have undergone periodontal treatment within the previous 6 months.
Show all 9Show fewer
- Patients presenting with acute dental pain (e.g., pulpitis or apical/periodontal abscess).
- Individuals who smoke or consume alcohol.
- Presence of fewer than 15 teeth.
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Faculty of Dentistry, Atatürk University
Erzurum, Erzurum
About this study
Official title: The Effect of Hypochlorous Acid in the Non-Surgical Treatment of Stage I and Stage II Periodontitis
Periodontitis is a chronic inflammatory disease associated with dental plaque and characterized by the progressive destruction of the tooth-supporting tissues. The principal features of periodontitis include clinical attachment loss (CAL), alveolar bone loss, periodontal pocket formation, gingival bleeding, and the subsequent loss of periodontal tissue support.
The current gold standard for the management of periodontal disease comprises oral hygiene instruction (OHI), non-surgical periodontal therapy (NSPT), periodontal surgery when indicated, and supportive periodontal therapy (SPT). In addition, various antimicrobial agents are used as adjuncts to periodontal treatment. One such agent, hypochlorous acid (HOCl), is a potent antimicrobial with high affinity against Gram-negative bacteria within the subgingival biofilm. Hypochlorous acid is commercially available in mouthwash and spray formulations, which patients may use as adjunctive therapies following periodontal treatment.
Read more
In the present project, hypochlorous acid will be applied by the clinician using a dental device during periodontal treatment in a clinical setting. Changes in both clinical and biochemical outcomes, including high-sensitivity C-reactive protein (hs-CRP), C-reactive protein (CRP), antistreptolysin O (ASO), and oncostatin M (OSM), will be recorded and compared with those obtained following conventional periodontal treatment performed using purified water.
The novelty of the proposed study lies in the first-time use of hypochlorous acid as an irrigant in place of purified water during scaling and root planing (SRP), with the aim of evaluating its effects on both clinical and biochemical parameters. This project is expected to attract the attention of researchers investigating the treatment of periodontitis and may facilitate publication in high-impact international peer-reviewed journals as well as presentation at prestigious scientific congresses. Furthermore, it will contribute to the training of qualified researchers in periodontology and support the completion of a doctoral dissertation. The findings may also provide preliminary data for future studies aimed at developing hypochlorous acid-based adjunctive therapeutic approaches for the management of periodontitis.
Read the detailed description
Periodontitis is a chronic inflammatory disease that may ultimately lead to tooth loss as a consequence of bacterial-induced destruction of the periodontal tissues. It is characterized by an inflammatory response to dental plaque within the periodontal connective tissue and alveolar bone, and the nature and magnitude of this host response largely determine disease progression. Scaling and root planing (SRP) is considered the gold standard for the treatment of periodontitis. The cornerstone of non-surgical periodontal therapy is the mechanical removal of microbial biofilm and calculus through SRP. However, SRP alone may not be sufficient to eliminate pathogenic bacteria, as these microorganisms can persist within the gingival tissues and in anatomical areas that are difficult to access using conventional periodontal instrumentation, including deep periodontal pockets, furcation involvements, interproximal areas, and root concavities. Therefore, local or systemic antimicrobial agents and antiseptics have been recommended as adjunctive therapies to improve periodontal treatment outcomes.
Owing to its antimicrobial, anti-inflammatory, and wound-healing properties, hypochlorous acid is currently used in various dental applications, including biofilm eradication, mouth rinses, wound care, endodontic irrigation, root surface conditioning, and the treatment of peri-implantitis.
This study has been designed as a single-center, randomized, controlled, prospective clinical trial to be conducted at the Department of Periodontology, Faculty of Dentistry, Atatürk University. A total of 44 systemically healthy individuals aged 18-65 years diagnosed with Stage I or Stage II periodontitis will be enrolled in the study.
Participants will be randomly allocated into two groups using a coin-toss randomization method:
Test group: Scaling and root planing (SRP) performed using hypochlorous acid. Control group: Scaling and root planing (SRP) performed using purified water.
All participants will receive standardized oral hygiene instructions at baseline. SRP will be performed using an ultrasonic device, with hypochlorous acid as the irrigating solution in the test group and purified water in the control group.
Clinical periodontal parameters will be recorded at baseline and at the 1-month and 3-month follow-up visits. These parameters will include:
Plaque Index (PI) Probing Pocket Depth (PPD) Bleeding on Probing (BOP) Clinical Attachment Level (CAL)
At the same time points, blood and saliva samples will be collected for the analysis of C-reactive protein (CRP), high-sensitivity C-reactive protein (hs-CRP), oncostatin M (OSM), and antistreptolysin O (ASO) using enzyme-linked immunosorbent assay (ELISA).
The primary objective of this study is to evaluate the clinical efficacy of hypochlorous acid as an adjunct to scaling and root planing. The secondary objective is to investigate its effects on systemic inflammatory biomarkers.
The findings of this study are expected to contribute to the current understanding of adjunctive therapeutic strategies in periodontal treatment and to support the clinical application of hypochlorous acid as a practical and effective adjunctive approach in the management of periodontitis.
- Conditions
- Periodontitis, Periodontitis Stage I, Periodontitis Stage II
- Being tested
- Hypochlorous Acid Application, Scaling and Root Planing (SRP) with Purified Water
- Keywords
- PERİODONTİTİS, CRP, hs-CRP, ONCOSTATİN M, Anti-streptolysin O, Hypochlorous acid, Non-surgical Periodontal Therapy
- Medical subject headings
- Periodontitis, Dry Socket
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Hypochlorous Acid ApplicationProcedure or surgery
Scaling and root planing (SRP) will be performed using an ultrasonic device with a hypochlorous acid solution.
Scaling and Root Planing (SRP) with Purified WaterProcedure or surgery
Scaling and root planing (SRP) will be performed using an ultrasonic device with purified water.
| Study group | Role | What they receive |
|---|---|---|
| Scaling and Root Planing (SRP) with Hypochlorous Acid | Experimental | Participants will undergo scaling and root planing (SRP) using hypochlorous acid. Receives: Procedure: Hypochlorous Acid Application |
| Scaling and Root Planing (SRP) with Purified Water | Active comparator | Participants will undergo scaling and root planing (SRP) using an ultrasonic device with purified water. Receives: Procedure: Scaling and Root Planing (SRP) with Purified Water |
What is being measured
The primary outcome is the main question the study is designed to answer.
Clinical Attachment Level (CAL)
Assessment of clinical attachment level (CAL), measured in millimeters, as an indicator of periodontal tissue healing.
Measured: Baseline, 1 month and 3 months
Probing Pocket Depth (PPD)
Assessment of probing pocket depth (PPD), measured in millimeters, to evaluate clinical periodontal healing.
Measured: Baseline, 1 month and 3 months
Plaque Index (PI)
Assessment of dental plaque accumulation.
Measured: Baseline, 1 month and 3 months
Bleeding on Probing (BOP)
Assessment of gingival bleeding following periodontal probing.
Measured: Baseline, 1 month and 3 months
Secondary outcomes (4)
C-Reactive Protein (CRP)
Assessment of the systemic inflammatory response by measuring serum C-reactive protein (CRP) levels.
Measured: Baseline, 1 month, and 3 months
High-Sensitivity C-Reactive Protein (hs-CRP)
Assessment of low-grade systemic inflammation by measuring high-sensitivity C-reactive protein (hs-CRP) levels.
Measured: Baseline, 1 month and 3 months
Oncostatin M (OSM)
Assessment of inflammatory cytokine levels associated with periodontal disease.
Measured: Baseline, 1 month and 3 months
Antistreptolysin O (ASO)
Assessment of the systemic immune response associated with streptococcal exposure by measuring antistreptolysin O (ASO) levels.
Measured: Baseline, 1 month and 3 months
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted17 Jul 2026
- Study start9 Oct 2025
- Primary completion15 May 2026
- Study completion12 Jun 2026
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for PeriodontitisStudies of the same condition, open and under way.
- More trials run by Ataturk UniversityThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Model description
- Participants will be randomly allocated into two parallel groups: a test group receiving scaling and root planing (SRP) using hypochlorous acid and a control group receiving scaling and root planing (SRP) using purified water.
- Masking
- Single blind
- Who is masked
- Outcomes assessor
- Enrolment
- 44 participants (actual)
Sponsor and investigators
- Lead sponsor
- Ataturk University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Alparslan Dilsiz, Ataturk University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07712627
- Sponsor's protocol ID
- ATAUNI-PERIO-HOCL-2025-001
- Other IDs
- BAP-16024
- Status last verified
- Jul 2026
- First submitted
- 14 Jun 2026
- Last updated
- 17 Jul 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No: Individual participant data (IPD) will not be shared due to confidentiality and privacy considerations. As this is a single-center study involving a limited number of participants, no formal data-sharing plan has been established.
Study at a glance
From the registry record, last updated by the sponsor on 17 Jul 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Effect of Hypochlorous Acid in the Non-Surgical Treatment of Stage I and Stage II Periodontitis
- ClinicalTrials.gov ID
- NCT07712627
- Acronym / sponsor ID
- ATAUNI-PERIO-HOCL-2025-001
- Conditions
- Periodontitis, Periodontitis Stage I, Periodontitis Stage II
- Keywords
- PERİODONTİTİS, CRP, hs-CRP, ONCOSTATİN M, Anti-streptolysin O, Hypochlorous acid, Non-surgical Periodontal Therapy
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 44 participants (actual)
- Lead sponsor
- Ataturk UniversityUniversity, hospital or other
- Study start
- 9 Oct 2025 (actual)
- Primary completion
- 15 May 2026 (actual)
- Study completion
- 12 Jun 2026 (actual)
- First posted
- 17 Jul 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Periodontitis is a chronic inflammatory disease associated with dental plaque and characterized by the progressive destruction of the tooth-supporting tissues. The principal features of periodontitis include clinical attachment loss (CAL), alveolar bone loss, periodontal pocket formation, gingival bleeding, and the subsequent loss of periodontal tissue support.
The current gold standard for the management of periodontal disease comprises oral hygiene instruction (OHI), non-surgical periodontal therapy (NSPT), periodontal surgery when indicated, and supportive periodontal therapy (SPT). In addition, various antimicrobial agents are used as adjuncts to periodontal treatment. One such agent, hypochlorous acid (HOCl), is a potent antimicrobial with high affinity against Gram-negative bacteria within the subgingival biofilm. Hypochlorous acid is commercially available in mouthwash and spray formulations, which patients may use as adjunctive therapies following periodontal treatment.
In the present project, hypochlorous acid will be applied by the clinician using a dental device during periodontal treatment in a clinical setting. Changes in both clinical and biochemical outcomes, including high-sensitivity C-reactive protein (hs-CRP), C-reactive protein (CRP), antistreptolysin O (ASO), and oncostatin M (OSM), will be recorded and compared with those obtained following conventional periodontal treatment performed using purified water.
The novelty of the proposed study lies in the first-time use of hypochlorous acid as an irrigant in place of purified water during scaling and root planing (SRP), with the aim of evaluating its effects on both clinical and biochemical parameters. This project is expected to attract the attention of researchers investigating the treatment of periodontitis and may facilitate publication in high-impact international peer-reviewed journals as well as presentation at prestigious scientific congresses. Furthermore, it will contribute to the training of qualified researchers in periodontology and support the completion of a doctoral dissertation. The findings may also provide preliminary data for future studies aimed at developing hypochlorous acid-based adjunctive therapeutic approaches for the management of periodontitis.
Detailed description
Periodontitis is a chronic inflammatory disease that may ultimately lead to tooth loss as a consequence of bacterial-induced destruction of the periodontal tissues. It is characterized by an inflammatory response to dental plaque within the periodontal connective tissue and alveolar bone, and the nature and magnitude of this host response largely determine disease progression. Scaling and root planing (SRP) is considered the gold standard for the treatment of periodontitis. The cornerstone of non-surgical periodontal therapy is the mechanical removal of microbial biofilm and calculus through SRP. However, SRP alone may not be sufficient to eliminate pathogenic bacteria, as these microorganisms can persist within the gingival tissues and in anatomical areas that are difficult to access using conventional periodontal instrumentation, including deep periodontal pockets, furcation involvements, interproximal areas, and root concavities. Therefore, local or systemic antimicrobial agents and antiseptics have been recommended as adjunctive therapies to improve periodontal treatment outcomes.
Owing to its antimicrobial, anti-inflammatory, and wound-healing properties, hypochlorous acid is currently used in various dental applications, including biofilm eradication, mouth rinses, wound care, endodontic irrigation, root surface conditioning, and the treatment of peri-implantitis.
This study has been designed as a single-center, randomized, controlled, prospective clinical trial to be conducted at the Department of Periodontology, Faculty of Dentistry, Atatürk University. A total of 44 systemically healthy individuals aged 18-65 years diagnosed with Stage I or Stage II periodontitis will be enrolled in the study.
Participants will be randomly allocated into two groups using a coin-toss randomization method:
Test group: Scaling and root planing (SRP) performed using hypochlorous acid. Control group: Scaling and root planing (SRP) performed using purified water.
All participants will receive standardized oral hygiene instructions at baseline. SRP will be performed using an ultrasonic device, with hypochlorous acid as the irrigating solution in the test group and purified water in the control group.
Clinical periodontal parameters will be recorded at baseline and at the 1-month and 3-month follow-up visits. These parameters will include:
Plaque Index (PI) Probing Pocket Depth (PPD) Bleeding on Probing (BOP) Clinical Attachment Level (CAL)
At the same time points, blood and saliva samples will be collected for the analysis of C-reactive protein (CRP), high-sensitivity C-reactive protein (hs-CRP), oncostatin M (OSM), and antistreptolysin O (ASO) using enzyme-linked immunosorbent assay (ELISA).
The primary objective of this study is to evaluate the clinical efficacy of hypochlorous acid as an adjunct to scaling and root planing. The secondary objective is to investigate its effects on systemic inflammatory biomarkers.
The findings of this study are expected to contribute to the current understanding of adjunctive therapeutic strategies in periodontal treatment and to support the clinical application of hypochlorous acid as a practical and effective adjunctive approach in the management of periodontitis.
Study design
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Single blind (outcomes assessor)
- Number of arms
- 2
- Model description
- Participants will be randomly allocated into two parallel groups: a test group receiving scaling and root planing (SRP) using hypochlorous acid and a control group receiving scaling and root planing (SRP) using purified water.
| Arm | Type | Receives |
|---|---|---|
| Scaling and Root Planing (SRP) with Hypochlorous Acid | Experimental | Procedure: Hypochlorous Acid ApplicationParticipants will undergo scaling and root planing (SRP) using hypochlorous acid. |
| Scaling and Root Planing (SRP) with Purified Water | Active comparator | Procedure: Scaling and Root Planing (SRP) with Purified WaterParticipants will undergo scaling and root planing (SRP) using an ultrasonic device with purified water. |
Who may qualify
- Ages
- 18 to 65 years
- Sex
- All
- Healthy volunteers
- Not accepted
- Age groups
- Adult, Older adult
Key inclusion criteria (6 listed)
- Individuals aged 18 to 65 years.
- Systemically healthy individuals.
- Diagnosis of Stage I or Stage II periodontitis according to the 2017 Classification of Periodontal Diseases.
- Probing pocket depth (PPD) ≤5 mm and clinical attachment loss (CAL) ≤4 mm.
- Presence of a sufficient number of teeth for periodontal evaluation.
Key exclusion criteria (9 listed)
- Patients with contraindications to the use of ultrasonic instrumentation.
- Pregnant or lactating women.
- Patients receiving anti-inflammatory or antibiotic therapy.
- Patients with systemic diseases or medical conditions.
- Patients receiving continuous medication for any medical condition.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Hypochlorous Acid ApplicationScaling and root planing (SRP) will be performed using an ultrasonic device with a hypochlorous acid solution. | Procedure or surgery | Scaling and Root Planing (SRP) with Hypochlorous Acid |
| Scaling and Root Planing (SRP) with Purified WaterScaling and root planing (SRP) will be performed using an ultrasonic device with purified water. | Procedure or surgery | Scaling and Root Planing (SRP) with Purified Water |
Primary outcome
Clinical Attachment Level (CAL)
Time frame: Baseline, 1 month and 3 months
Assessment of clinical attachment level (CAL), measured in millimeters, as an indicator of periodontal tissue healing.
Probing Pocket Depth (PPD)
Time frame: Baseline, 1 month and 3 months
Assessment of probing pocket depth (PPD), measured in millimeters, to evaluate clinical periodontal healing.
Plaque Index (PI)
Time frame: Baseline, 1 month and 3 months
Assessment of dental plaque accumulation.
Bleeding on Probing (BOP)
Time frame: Baseline, 1 month and 3 months
Assessment of gingival bleeding following periodontal probing.
4 secondary outcomes
C-Reactive Protein (CRP)
Time frame: Baseline, 1 month, and 3 months
High-Sensitivity C-Reactive Protein (hs-CRP)
Time frame: Baseline, 1 month and 3 months
Oncostatin M (OSM)
Time frame: Baseline, 1 month and 3 months
Antistreptolysin O (ASO)
Time frame: Baseline, 1 month and 3 months
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 17 Jul 2026.
- Provides general information about the hypochlorous acid used in this study.Link provided by the sponsor.
- Provides general information about periodontitis.Link provided by the sponsor.
- Presents the current classification of periodontal disease used as the basis for participant eligibility in this study.Link provided by the sponsor.
- Individual participant dataSharing planned: No. Individual participant data (IPD) will not be shared due to confidentiality and privacy considerations. As this is a single-center study involving a limited number of participants, no formal data-sharing plan has been established.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Hypochlorous Acid Application and Scaling and Root Planing (SRP) with Purified Water in people with Periodontitis and Periodontitis Stage I, run by Ataturk University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 6 inclusion and 9 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 17 Jul 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Clinical Attachment Level (CAL) (time frame: Baseline, 1 month and 3 months). The study lists 4 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 17 Jul 2026.