ExerciseRWT-THYROIDNCT07765290
Thyroid Hormone Responses to Repeated Wingate Exercise Versus a Non-Exercising Comparison Group
A study testing Repeated Wingate Exercise in people with Exercise and Thyroid Function, run by Inonu University at 1 site in Turkey (Türkiye).
Trial summary
This two-group, non-randomized study investigated the acute effects of repeated Wingate exercise on free thyroid hormone concentrations in male amateur soccer players. Twenty healthy male amateur soccer players completed a standardized repeated Wingate exercise protocol, and a separate group of twenty male amateur… Read more about this study
- Who is it for?
- Ages 18 to 25 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Exercise, Thyroid Function
- What is being tested?
- Repeated Wingate Exercise
- Type of treatment
- Lifestyle or therapy programme
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 40 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 1 Aug 2024to 27 Aug 2024
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 25 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Male amateur soccer players.
- Age between 18 and 25 years.
- At least 6 years of licensed soccer experience.
- Actively participating in amateur soccer training.
- Apparently healthy and free from acute or chronic disease on medical history review.
- Willingness to participate and provision of written informed consent.
Who cannot join
- Acute illness or musculoskeletal injury at the time of testing.
- Neurological, auditory, visual or vestibular disorder.
- Reduced physical capacity due to injury, or a sedentary lifestyle.
- History of nutritional or pharmacological ergogenic aid use.
- Failure to complete the study procedures or all required blood sample collections.
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Exercise Physiology Laboratory, Faculty of Sport Sciences, Karabuk University
Karabük, Karabük Province, 78050
About this study
Official title: Free Thyroid Hormone Responses to Repeated Wingate Exercise Versus a Non-Exercising Comparison Group in Male Amateur Soccer Players
This two-group, non-randomized study investigated the acute effects of repeated Wingate exercise on free thyroid hormone concentrations in male amateur soccer players. Twenty healthy male amateur soccer players completed a standardized repeated Wingate exercise protocol, and a separate group of twenty male amateur soccer players provided two morning blood samples without exercising. Blood samples were collected immediately before and immediately after the exercise session or the resting interval to determine free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations. Including a non-exercising comparison group allows the observed changes to be evaluated against the change expected across a comparable interval in the absence of exercise.
Read the detailed description
High-intensity anaerobic exercise induces acute physiological and endocrine responses that contribute to metabolic regulation and athletic performance. Thyroid hormones, including free triiodothyronine (fT3) and free thyroxine (fT4), play essential roles in energy metabolism, oxygen consumption, and exercise adaptation. However, the immediate effects of repeated supramaximal exercise on thyroid hormone responses remain insufficiently understood, and most previous reports lack any non-exercising comparison condition, so observed changes cannot be separated from time of day, posture, repeated venipuncture, or routine analytical variation.
This two-group non-randomized study was designed to compare the acute effects of repeated Wingate exercise on free thyroid hormone concentrations against a non-exercising comparison group. Twenty healthy male amateur soccer players completed a standardized repeated Wingate exercise protocol under controlled laboratory conditions, consisting of six 30-second maximal bouts at 7.5% of body mass separated by 4 minutes of passive recovery. A comparison group of twenty male amateur soccer players provided two venous blood samples on one morning without performing any exercise between them. Venous blood samples were collected immediately before and immediately after the exercise session or the resting interval to determine free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations. Hormone analyses were performed using standardized laboratory procedures.
The two groups were recruited and sampled in different periods rather than concurrently, and participants were therefore not randomly allocated. The primary objective was to determine whether the change in circulating free thyroid hormone concentrations around a repeated Wingate session differs from the change observed across a comparable resting interval.
- Conditions
- Exercise, Thyroid Function
- Being tested
- Repeated Wingate Exercise
- Keywords
- free triiodothyronine, free thyroxine, thyroid-stimulating hormone, high-intensity interval exercise, Wingate anaerobic test, soccer, acute endocrine response
- Medical subject headings
- Motor Activity
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Repeated Wingate ExerciseBehavioural
Also called Repeated Wingate Test
Participants completed a standardized repeated Wingate exercise protocol under controlled laboratory conditions to investigate the acute effects of high-intensity anaerobic exercise on thyroid hormone responses. Venous blood samples were collected immediately before and immediately after the exercise session to measure serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations.
| Study group | Role | What they receive |
|---|---|---|
| Repeated Wingate Exercise | Experimental | Participants completed a standardized repeated Wingate exercise protocol under laboratory conditions. Blood samples were collected immediately before and immediately after the exercise session to assess acute changes in serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH). Receives: Behavioral: Repeated Wingate Exercise |
| Comparison group | No intervention | Participants provided two venous blood samples on one morning and performed no exercise between the two samples. Serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) were measured in both samples. |
What is being measured
The primary outcome is the main question the study is designed to answer.
Change in Serum Free Thyroid Hormone Concentrations (fT3, fT4, and TSH)
The primary outcome was the within-participant change in serum free thyroid hormone concentrations, including free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH), measured immediately before and immediately after the repeated Wingate exercise protocol in the exercise group and across a comparable resting interval in the comparison group. Change scores were compared between groups.
Measured: Immediately before and immediately after the repeated Wingate exercise session or the resting interval
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted14 Aug 2026
- Study start1 Aug 2024
- Primary completion27 Aug 2024
- Study completion27 Aug 2024
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for ExerciseStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Model description
- This study used a two-group, non-randomized, non-concurrent design. One group completed a repeated Wingate exercise protocol; a separate comparison group provided two morning blood samples without exercising. Serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations were measured immediately before and immediately after the exercise protocol or the resting interval. The two groups were recruited and sampled in different periods rather than concurrently, so participants were not randomly allocated to conditions.
- Masking
- None (open label)
- Enrolment
- 40 participants (actual)
Sponsor and investigators
- Lead sponsor
- Inonu University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Assoc. Prof. Dr. Özgür EKEN, Inonu University
- Investigators
- Özgür EKEN, PhD, Principal investigator, Inonu University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07765290
- Sponsor's protocol ID
- KBU-HIIT-2023-01
- Acronym
- RWT-THYROID
- Status last verified
- Oct 2026
- First submitted
- 11 Aug 2026
- Last updated
- 19 Oct 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- Yes: De-identified participant-level data and analysis code are available from the corresponding author on reasonable request, subject to applicable ethics, participant-consent, and institutional requirements.
Study at a glance
From the registry record, last updated by the sponsor on 19 Oct 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Free Thyroid Hormone Responses to Repeated Wingate Exercise Versus a Non-Exercising Comparison Group in Male Amateur Soccer Players
- ClinicalTrials.gov ID
- NCT07765290
- Acronym / sponsor ID
- RWT-THYROID / KBU-HIIT-2023-01
- Conditions
- Exercise, Thyroid Function
- Keywords
- free triiodothyronine, free thyroxine, thyroid-stimulating hormone, high-intensity interval exercise, Wingate anaerobic test, soccer, acute endocrine response
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 40 participants (actual)
- Lead sponsor
- Inonu UniversityUniversity, hospital or other
- Study start
- 1 Aug 2024 (actual)
- Primary completion
- 27 Aug 2024 (actual)
- Study completion
- 27 Aug 2024 (actual)
- First posted
- 14 Aug 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This two-group, non-randomized study investigated the acute effects of repeated Wingate exercise on free thyroid hormone concentrations in male amateur soccer players. Twenty healthy male amateur soccer players completed a standardized repeated Wingate exercise protocol, and a separate group of twenty male amateur soccer players provided two morning blood samples without exercising. Blood samples were collected immediately before and immediately after the exercise session or the resting interval to determine free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations. Including a non-exercising comparison group allows the observed changes to be evaluated against the change expected across a comparable interval in the absence of exercise.
Detailed description
High-intensity anaerobic exercise induces acute physiological and endocrine responses that contribute to metabolic regulation and athletic performance. Thyroid hormones, including free triiodothyronine (fT3) and free thyroxine (fT4), play essential roles in energy metabolism, oxygen consumption, and exercise adaptation. However, the immediate effects of repeated supramaximal exercise on thyroid hormone responses remain insufficiently understood, and most previous reports lack any non-exercising comparison condition, so observed changes cannot be separated from time of day, posture, repeated venipuncture, or routine analytical variation.
This two-group non-randomized study was designed to compare the acute effects of repeated Wingate exercise on free thyroid hormone concentrations against a non-exercising comparison group. Twenty healthy male amateur soccer players completed a standardized repeated Wingate exercise protocol under controlled laboratory conditions, consisting of six 30-second maximal bouts at 7.5% of body mass separated by 4 minutes of passive recovery. A comparison group of twenty male amateur soccer players provided two venous blood samples on one morning without performing any exercise between them. Venous blood samples were collected immediately before and immediately after the exercise session or the resting interval to determine free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations. Hormone analyses were performed using standardized laboratory procedures.
The two groups were recruited and sampled in different periods rather than concurrently, and participants were therefore not randomly allocated. The primary objective was to determine whether the change in circulating free thyroid hormone concentrations around a repeated Wingate session differs from the change observed across a comparable resting interval.
Study design
- Primary purpose
- Basic science
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Number of arms
- 2
- Model description
- This study used a two-group, non-randomized, non-concurrent design. One group completed a repeated Wingate exercise protocol; a separate comparison group provided two morning blood samples without exercising. Serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations were measured immediately before and immediately after the exercise protocol or the resting interval. The two groups were recruited and sampled in different periods rather than concurrently, so participants were not randomly allocated to conditions.
| Arm | Type | Receives |
|---|---|---|
| Repeated Wingate Exercise | Experimental | Behavioral: Repeated Wingate ExerciseParticipants completed a standardized repeated Wingate exercise protocol under laboratory conditions. Blood samples were collected immediately before and immediately after the exercise session to assess acute changes in serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH). |
| Comparison group | No intervention | –Participants provided two venous blood samples on one morning and performed no exercise between the two samples. Serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) were measured in both samples. |
Who may qualify
- Ages
- 18 to 25 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (6 listed)
- Male amateur soccer players.
- Age between 18 and 25 years.
- At least 6 years of licensed soccer experience.
- Actively participating in amateur soccer training.
- Apparently healthy and free from acute or chronic disease on medical history review.
Key exclusion criteria (5 listed)
- Acute illness or musculoskeletal injury at the time of testing.
- Neurological, auditory, visual or vestibular disorder.
- Reduced physical capacity due to injury, or a sedentary lifestyle.
- History of nutritional or pharmacological ergogenic aid use.
- Failure to complete the study procedures or all required blood sample collections.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Repeated Wingate ExerciseAlso: Repeated Wingate TestParticipants completed a standardized repeated Wingate exercise protocol under controlled laboratory conditions to investigate the acute effects of high-intensity anaerobic exercise on thyroid hormone responses. Venous blood samples were collected immediately before and immediately after the exercise session to measure serum free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH) concentrations. | Behavioural | Repeated Wingate Exercise |
Primary outcome
Change in Serum Free Thyroid Hormone Concentrations (fT3, fT4, and TSH)
Time frame: Immediately before and immediately after the repeated Wingate exercise session or the resting interval
The primary outcome was the within-participant change in serum free thyroid hormone concentrations, including free triiodothyronine (fT3), free thyroxine (fT4), and thyroid-stimulating hormone (TSH), measured immediately before and immediately after the repeated Wingate exercise protocol in the exercise group and across a comparable resting interval in the comparison group. Change scores were compared between groups.
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 19 Oct 2026.
- Individual participant dataSharing planned: Yes. De-identified participant-level data and analysis code are available from the corresponding author on reasonable request, subject to applicable ethics, participant-consent, and institutional requirements.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Özgür EKEN, PhD principal investigator
Inonu University
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Repeated Wingate Exercise in people with Exercise and Thyroid Function, run by Inonu University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 6 inclusion and 5 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 19 Oct 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Change in Serum Free Thyroid Hormone Concentrations (fT3, fT4, and TSH) (time frame: Immediately before and immediately after the repeated Wingate exercise session or the resting interval). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 19 Oct 2026.