Healthy VolunteersNCT07819422
Acute Effects of HIIT and MICT on Catestatin Dynamics in Young Males
A study testing High-Intensity Interval Exercise and Moderate-Intensity Continuous Exercise in people with Healthy Volunteers and Exercise Physiology, run by Firat University at 1 site in Turkey (Türkiye).
Trial summary
Physical exercise acts as a major physiological stressor that alters cardiovascular and autonomic homeostasis. Catestatin (CTS), a bioactive peptide derived from chromogranin A (CgA), acts as a potent inhibitor of catecholamine secretion and modulates autonomic cardiovascular tone. Read more about this study
- Who is it for?
- Ages 18 to 25 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Healthy Volunteers, Exercise Physiology, Autonomic Nervous System Regulation
- What is being tested?
- High-Intensity Interval Exercise and Moderate-Intensity Continuous Exercise
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 20 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 24 Oct 2025to 5 Mar 2026
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 25 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Healthy male individuals aged 18-25 years
- Body mass index (BMI) between 18.0 and 25.0 kg/m²
- Sedentary lifestyle (no regular exercise engagement within the last 3 months; <90 min/week moderate-intensity or <60 min/week vigorous-intensity exercise)
- Normal resting blood pressure (<140/90 mmHg)
- Fasting plasma glucose between 60 and 100 mg/dL
- Normal resting electrocardiogram (ECG) and pulmonary function tests (FEV1/FVC >80%)
- Signed written informed consent
Who cannot join
- Regular smoking or alcohol consumption
- Any current or regular medication use
- History or clinical signs of cardiovascular, respiratory, metabolic, or renal diseases
- Symptoms suggestive of cardiopulmonary/metabolic disease (chest pain, shortness of breath, dizziness, syncope, ankle edema, palpitations, intermittent claudication)
- Presence of known cardiovascular risk factors
- Iron deficiency / anemia
- Musculoskeletal limitations or contraindications to maximal cycle ergometer testing
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Firat University Medical Faculty, Department of Physiology
Elâzığ, Elâzığ, 23119
About this study
Official title: Effects of Acute HIIT and MICT on Catestatin Dynamics in Sedentary Young Males: Selective Association With Post-Exercise Autonomic Recovery
Physical exercise acts as a major physiological stressor that alters cardiovascular and autonomic homeostasis. Catestatin (CTS), a bioactive peptide derived from chromogranin A (CgA), acts as a potent inhibitor of catecholamine secretion and modulates autonomic cardiovascular tone. However, the acute effects of distinct exercise modalities on circulating CTS dynamics and their associations with post-exercise autonomic recovery remain unclarified.
The purpose of this study is to investigate the acute responses of serum catestatin and chromogranin A to high-intensity interval training (HIIT) versus moderate-intensity continuous training (MICT) in healthy sedentary young males, and to evaluate their potential relationship with post-exercise heart rate recovery (HRR). Participants completed an initial incremental performance test followed by either a single HIIT session or a MICT session on a cycle ergometer. Serial blood samples and continuous cardiovascular measurements were obtained before, immediately after, and throughout a 60-minute post-exercise recovery period.
Read the detailed description
Twenty healthy sedentary young adult males (aged 18-25 years) were assigned to either a high-intensity interval training group (HIIT, n=10) or a moderate-intensity continuous training group (MICT, n=10). Following an initial incremental maximal exercise test to determine individual exercise capacities, participants performed their assigned acute exercise protocol on a cycle ergometer separated by at least 48 hours.
The MICT session consisted of 30 minutes of continuous cycling at 60-70% of maximum heart rate (HRmax), whereas the HIIT protocol comprised four 4-minute intervals at 85-95% HRmax interleaved with 3-minute active recovery periods. Serial venous blood samples were collected at baseline (Pre), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise to analyze serum catestatin and chromogranin A concentrations via enzyme-linked immunosorbent assay (ELISA). Continuous heart rate and intermittent blood pressure were monitored throughout the exercise bouts and during the 60-minute recovery phase. Post-exercise autonomic regulation was assessed using 1-minute (HRR1) and 5-minute (HRR5) heart rate recovery indices.
- Being tested
- High-Intensity Interval Exercise, Moderate-Intensity Continuous Exercise
- Keywords
- Catestatin, Chromogranin A, High-intensity interval training, Moderate-intensity continuous training, Heart rate recovery, Parasympathetic reactivation
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
High-Intensity Interval ExerciseOther
Acute bout of 4x4 min intervals at 85-95% HRmax with 3 min active recovery on a cycle ergometer.
Moderate-Intensity Continuous ExerciseOther
Acute bout of continuous cycling for 30 minutes at 60-70% HRmax on a cycle ergometer.
| Study group | Role | What they receive |
|---|---|---|
| High-Intensity Interval Training (HIIT) | Experimental | Participants completed an acute HIIT session on a cycle ergometer consisting of a 5-minute warm-up, followed by four 4-minute bouts at 85-95% of maximum heart rate (HRmax) interleaved with 3-minute active recovery periods, followed by a 5-minute cool-down. Receives: Other: High-Intensity Interval Exercise |
| Moderate-Intensity Continuous Training (MICT) | Experimental | Participants completed an acute MICT session on a cycle ergometer consisting of a 5-minute warm-up, followed by 30 minutes of continuous cycling at 60-70% of maximum heart rate (HRmax), followed by a 5-minute cool-down. Receives: Other: Moderate-Intensity Continuous Exercise |
What is being measured
The primary outcome is the main question the study is designed to answer.
Serum Catestatin Concentration
Serum catestatin levels measured using enzyme-linked immunosorbent assay (ELISA) to evaluate acute exercise-induced dynamic responses.
Measured: Baseline (pre-exercise), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise.
Secondary outcomes (2)
Serum Chromogranin-A Concentration
Serum chromogranin-A (CgA) levels measured via ELISA to assess precursor dynamics and neuroendocrine response across exercise modalities.
Measured: Baseline (pre-exercise), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise.
Post-Exercise Heart Rate Recovery (HRR1 and HRR5)
Heart rate recovery calculated at 1 minute (HRR1 = peak heart rate minus heart rate at 1 min post-exercise) and 5 minutes (HRR5 = peak heart rate minus heart rate at 5 min post-exercise) to evaluate parasympathetic reactivation and sympathetic withdrawal.
Measured: At 1 minute and 5 minutes post-exercise.
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted15 Sep 2026
- Study start24 Oct 2025
- Primary completion10 Jan 2026
- Study completion5 Mar 2026
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Healthy VolunteersStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Model description
- A two-arm parallel-group design comparing acute high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) in sedentary young males.
- Masking
- None (open label)
- Enrolment
- 20 participants (actual)
Sponsor and investigators
- Lead sponsor
- Firat University
- Sponsor type
- University, hospital or other
- Collaborators
- The Scientific and Technological Research Council of Turkey
- Responsible party
- Principal investigator, Orhan Sayın, Firat University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07819422
- Sponsor's protocol ID
- 2025/09-13
- Other IDs
- 125S394
- Status last verified
- Jul 2025
- First submitted
- 9 Sep 2026
- Last updated
- 15 Sep 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- Yes: De-identified individual participant data that underlie the results reported in this article will be shared with researchers upon reasonable request directed to the corresponding author.
Study at a glance
From the registry record, last updated by the sponsor on 15 Sep 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Effects of Acute HIIT and MICT on Catestatin Dynamics in Sedentary Young Males: Selective Association With Post-Exercise Autonomic Recovery
- ClinicalTrials.gov ID
- NCT07819422
- Acronym / sponsor ID
- 2025/09-13
- Keywords
- Catestatin, Chromogranin A, High-intensity interval training, Moderate-intensity continuous training, Heart rate recovery, Parasympathetic reactivation
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 20 participants (actual)
- Lead sponsor
- Firat UniversityUniversity, hospital or other
- Collaborators
- The Scientific and Technological Research Council of Turkey
- Study start
- 24 Oct 2025 (actual)
- Primary completion
- 10 Jan 2026 (actual)
- Study completion
- 5 Mar 2026 (actual)
- First posted
- 15 Sep 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Physical exercise acts as a major physiological stressor that alters cardiovascular and autonomic homeostasis. Catestatin (CTS), a bioactive peptide derived from chromogranin A (CgA), acts as a potent inhibitor of catecholamine secretion and modulates autonomic cardiovascular tone. However, the acute effects of distinct exercise modalities on circulating CTS dynamics and their associations with post-exercise autonomic recovery remain unclarified.
The purpose of this study is to investigate the acute responses of serum catestatin and chromogranin A to high-intensity interval training (HIIT) versus moderate-intensity continuous training (MICT) in healthy sedentary young males, and to evaluate their potential relationship with post-exercise heart rate recovery (HRR). Participants completed an initial incremental performance test followed by either a single HIIT session or a MICT session on a cycle ergometer. Serial blood samples and continuous cardiovascular measurements were obtained before, immediately after, and throughout a 60-minute post-exercise recovery period.
Detailed description
Twenty healthy sedentary young adult males (aged 18-25 years) were assigned to either a high-intensity interval training group (HIIT, n=10) or a moderate-intensity continuous training group (MICT, n=10). Following an initial incremental maximal exercise test to determine individual exercise capacities, participants performed their assigned acute exercise protocol on a cycle ergometer separated by at least 48 hours.
The MICT session consisted of 30 minutes of continuous cycling at 60-70% of maximum heart rate (HRmax), whereas the HIIT protocol comprised four 4-minute intervals at 85-95% HRmax interleaved with 3-minute active recovery periods. Serial venous blood samples were collected at baseline (Pre), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise to analyze serum catestatin and chromogranin A concentrations via enzyme-linked immunosorbent assay (ELISA). Continuous heart rate and intermittent blood pressure were monitored throughout the exercise bouts and during the 60-minute recovery phase. Post-exercise autonomic regulation was assessed using 1-minute (HRR1) and 5-minute (HRR5) heart rate recovery indices.
Study design
- Primary purpose
- Basic science
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Number of arms
- 2
- Model description
- A two-arm parallel-group design comparing acute high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) in sedentary young males.
| Arm | Type | Receives |
|---|---|---|
| High-Intensity Interval Training (HIIT) | Experimental | Other: High-Intensity Interval ExerciseParticipants completed an acute HIIT session on a cycle ergometer consisting of a 5-minute warm-up, followed by four 4-minute bouts at 85-95% of maximum heart rate (HRmax) interleaved with 3-minute active recovery periods, followed by a 5-minute cool-down. |
| Moderate-Intensity Continuous Training (MICT) | Experimental | Other: Moderate-Intensity Continuous ExerciseParticipants completed an acute MICT session on a cycle ergometer consisting of a 5-minute warm-up, followed by 30 minutes of continuous cycling at 60-70% of maximum heart rate (HRmax), followed by a 5-minute cool-down. |
Who may qualify
- Ages
- 18 to 25 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (7 listed)
- Healthy male individuals aged 18-25 years
- Body mass index (BMI) between 18.0 and 25.0 kg/m²
- Sedentary lifestyle (no regular exercise engagement within the last 3 months; <90 min/week moderate-intensity or <60 min/week vigorous-intensity exercise)
- Normal resting blood pressure (<140/90 mmHg)
- Fasting plasma glucose between 60 and 100 mg/dL
Key exclusion criteria (7 listed)
- Regular smoking or alcohol consumption
- Any current or regular medication use
- History or clinical signs of cardiovascular, respiratory, metabolic, or renal diseases
- Symptoms suggestive of cardiopulmonary/metabolic disease (chest pain, shortness of breath, dizziness, syncope, ankle edema, palpitations, intermittent claudication)
- Presence of known cardiovascular risk factors
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| High-Intensity Interval ExerciseAcute bout of 4x4 min intervals at 85-95% HRmax with 3 min active recovery on a cycle ergometer. | Other | High-Intensity Interval Training (HIIT) |
| Moderate-Intensity Continuous ExerciseAcute bout of continuous cycling for 30 minutes at 60-70% HRmax on a cycle ergometer. | Other | Moderate-Intensity Continuous Training (MICT) |
Primary outcome
Serum Catestatin Concentration
Time frame: Baseline (pre-exercise), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise.
Serum catestatin levels measured using enzyme-linked immunosorbent assay (ELISA) to evaluate acute exercise-induced dynamic responses.
2 secondary outcomes
Serum Chromogranin-A Concentration
Time frame: Baseline (pre-exercise), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise.
Post-Exercise Heart Rate Recovery (HRR1 and HRR5)
Time frame: At 1 minute and 5 minutes post-exercise.
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 15 Sep 2026.
- Individual participant dataSharing planned: Yes. De-identified individual participant data that underlie the results reported in this article will be shared with researchers upon reasonable request directed to the corresponding author.
Related trials for Healthy Volunteers
Not yet recruiting
Neuroergonomic Analysis of a Brain-Computer Interface Device (BCI)
Healthy Volunteers - Male and Female
RecruitingPhase 1
First-in-Human Trial to Assess REGN20423 in Healthy Adult Participants and Adult Participants With Atopic Dermatitis
Healthy Volunteer, Atopic Dermatitis
Not yet recruitingObservational study
Establishment of Biobank of Nasal Epithelium Samples From Healthy Volunteers
Healthy
RecruitingObservational study
Improved Methods of Cell Selection for Bone Marrow Transplant Alternatives
Graft-Versus-Host Disease, Graft-versus-leukemia
Not yet recruitingObservational study
Screening Protocol
Pain
Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing High-Intensity Interval Exercise and Moderate-Intensity Continuous Exercise in people with Healthy Volunteers and Exercise Physiology, run by Firat University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 7 inclusion and 7 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 15 Sep 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Serum Catestatin Concentration (time frame: Baseline (pre-exercise), immediately post-exercise (0 min), and at 15, 30, 45, and 60 minutes post-exercise.). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 15 Sep 2026.