Immune Function Sedentary LifestyleCFIT-IMMUNENCT07824830
Effects of CrossFit Training on the Immune System
A study testing CrossFit Training Program in people with Immune Function Sedentary Lifestyle, run by Inonu University at 1 site in Turkey (Türkiye).
Trial summary
This interventional study aims to investigate the effects of both acute and eight-week CrossFit training on immune system responses in sedentary adults. Participants will undergo an acute CrossFit exercise session, followed by an eight-week supervised CrossFit training program. Read more about this study
- Who is it for?
- Ages 19 to 24 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Immune Function Sedentary Lifestyle
- What is being tested?
- CrossFit Training Program
- Type of treatment
- Lifestyle or therapy programme
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 30 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 5 May 2026to 30 Jun 2026
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 19 to 24 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Currently enrolled as a student at Yalova University
- Not regularly participating in exercise or sports training
- Medically eligible to participate in CrossFit training
- Willing and able to complete the eight-week CrossFit training program conducted three days per week
- Willing to provide venous blood samples at the specified assessment times
- Willing to provide written informed consent
Show all 13Show fewer
- Exclusion Criteria:- Regular participation in exercise or sports training
- History of a neurological disorder within the previous year
- History of an auditory, visual, or vestibular disorder within the previous year
- Serious upper- or lower-extremity injury within the previous six months
- Presence of a medical condition that could prevent safe participation in the exercise program
- Inability to complete the training or blood-sampling procedures
- Withdrawal of voluntary consent
Who cannot join
None listed.
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Exercise Physiology Laboratory, Faculty of Sport Sciences, Karabuk University
Karabük, Karabük Province
About this study
Official title: From Acute Response to Chronic Adaptation: Immunological Effects of an Eight-Week CrossFit Training Program in Sedentary Adults
This interventional study aims to investigate the effects of both acute and eight-week CrossFit training on immune system responses in sedentary adults. Participants will undergo an acute CrossFit exercise session, followed by an eight-week supervised CrossFit training program. Blood samples will be collected before and after the acute exercise session and again following the eight-week intervention to evaluate changes in selected immune system biomarkers. The primary objective is to determine whether acute and chronic CrossFit training induces significant alterations in immune function in previously sedentary individuals. The findings are expected to contribute to a better understanding of the immunological adaptations associated with high-intensity functional training and to provide evidence regarding the safety and potential health benefits of CrossFit exercise in sedentary populations.
Read the detailed description
CrossFit is a high-intensity functional training (HIFT) program that combines aerobic exercise, resistance training, weightlifting, gymnastics, and metabolic conditioning within a single exercise session. Owing to its increasing popularity among both athletes and the general population, there is growing interest in understanding the physiological and immunological adaptations associated with CrossFit training. Although regular physical activity is known to enhance immune function and improve overall health, high-intensity exercise may induce transient alterations in immune responses, particularly in previously sedentary individuals.
The immune system plays a critical role in protecting the body against infections and maintaining physiological homeostasis. Acute bouts of strenuous exercise may temporarily modify immune cell function and inflammatory responses, whereas long-term exercise training may promote beneficial adaptations and improve immune regulation. However, the effects of CrossFit training on immune system biomarkers in sedentary adults remain insufficiently investigated.
The purpose of this interventional study is to evaluate the effects of both an acute CrossFit exercise session and an eight-week supervised CrossFit training program on immune system responses in sedentary adults. Participants will complete baseline assessments followed by an acute CrossFit exercise session. Subsequently, they will participate in an eight-week supervised CrossFit training program. Blood samples will be collected at predetermined time points before and after the acute exercise session and following completion of the eight-week intervention to assess changes in immune system biomarkers.
The primary objective is to determine whether acute and chronic CrossFit training produce significant changes in immune function in sedentary individuals. The results of this study are expected to improve understanding of the immunological responses to high-intensity functional training and provide evidence regarding the safety and potential health benefits of CrossFit exercise for previously sedentary adults.
- Conditions
- Immune Function Sedentary Lifestyle
- Being tested
- CrossFit Training Program
- Keywords
- CrossFit, Immune System, Acute Exercise, Chronic Exercise
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
CrossFit Training ProgramBehavioural
Also called 8-Week CrossFit Training Program
articipants in the experimental group will participate in an eight-week CrossFit training program performed three days per week (Monday, Wednesday, and Friday). Each training session will last approximately 60 minutes and will include a dynamic warm-up, strength training exercises, a Workout of the Day (WOD) section, and a cool-down period.
The strength training component will include functional resistance exercises such as squats, deadlifts, and push presses, performed for 3-5 sets of 5-10 repetitions. Training intensity will progressively increase from approximately 60-70% of one-repetition maximum (1RM) during the first four weeks to 70-80% of 1RM during the final four weeks.
The WOD component will include high-intensity functional exercises such as burpees, kettlebell swings, box jumps, rowing, sit-ups, pull-ups, and short-distance running. WOD sessions will be performed using AMRAP (As Many Rounds As Possible) and For Time formats with durations of approximately 10-20 minutes. P
| Study group | Role | What they receive |
|---|---|---|
| CrossFit Training | Experimental | Participants assigned to the CrossFit Training Group will participate in a structured CrossFit exercise program for 8 weeks. Training sessions will be performed three times per week under the supervision of certified trainers. Each session will include warm-up, functional strength exercises, high-intensity metabolic conditioning exercises, and cool-down periods. Exercise intensity will be monitored throughout the intervention period. Receives: Behavioral: CrossFit Training Program |
What is being measured
The primary outcome is the main question the study is designed to answer.
Mean Change in Total Leukocyte Count From Baseline to Completion of the Eight-Week CrossFit Program
Total leukocyte count will be determined from venous blood samples collected before the first training session and immediately after completion of the eight-week CrossFit training program. For each participant, the change will be calculated as the post-program value minus the baseline value. The group-level outcome will be summarized using the mean change and standard deviation.
Measured: Baseline and immediately after completion of the eight-week CrossFit training program
Mean Change in Absolute Monocyte Count From Baseline to Completion of the Eight-Week CrossFit Program
Absolute monocyte count will be determined from venous blood samples collected before the first training session and immediately after completion of the eight-week CrossFit training program. For each participant, the change will be calculated as the post-program value minus the baseline value. The group-level outcome will be summarized using the mean change and standard deviation.
Measured: Baseline and immediately after completion of the eight-week CrossFit training program
Mean Change in Absolute Lymphocyte Count From Baseline to Completion of the Eight-Week CrossFit Program
Absolute lymphocyte count will be determined from venous blood samples collected before the first training session and immediately after completion of the eight-week CrossFit training program. For each participant, the change will be calculated as the post-program value minus the baseline value. The group-level outcome will be summarized using the mean change and standard deviation.
Measured: Baseline and immediately after completion of the eight-week CrossFit training program
Mean Change in Absolute Neutrophil Count From Baseline to Completion of the Eight-Week CrossFit Program
Absolute neutrophil count will be determined from venous blood samples collected before the first training session and immediately after completion of the eight-week CrossFit training program. For each participant, the change will be calculated as the post-program value minus the baseline value. The group-level outcome will be summarized using the mean change and standard deviation.
Measured: Baseline and immediately after completion of the eight-week CrossFit training program
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted17 Sep 2026
- Study start5 May 2026
- Primary completion30 Jun 2026
- Study completion30 Jun 2026
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Immune Function Sedentary LifestyleStudies of the same condition, open and under way.
- More trials run by Inonu UniversityThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Model description
- All participants were assigned to a single intervention group and completed an acute CrossFit exercise session followed by an eight-week supervised CrossFit training program. Immune system biomarkers were assessed at baseline, immediately after the acute exercise session, and after completion of the eight-week intervention to evaluate acute and chronic immunological responses.
- Masking
- None (open label)
- Enrolment
- 30 participants (actual)
Sponsor and investigators
- Lead sponsor
- Inonu University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Assoc. Prof. Dr. Özgür EKEN, Inonu University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07824830
- Sponsor's protocol ID
- KBU-FTB12-2026-002
- Acronym
- CFIT-IMMUNE
- Status last verified
- Sep 2026
- First submitted
- 28 Jul 2026
- Last updated
- 17 Sep 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Study at a glance
From the registry record, last updated by the sponsor on 17 Sep 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- From Acute Response to Chronic Adaptation: Immunological Effects of an Eight-Week CrossFit Training Program in Sedentary Adults
- ClinicalTrials.gov ID
- NCT07824830
- Acronym / sponsor ID
- CFIT-IMMUNE / KBU-FTB12-2026-002
- Conditions
- Immune Function Sedentary Lifestyle
- Keywords
- CrossFit, Immune System, Acute Exercise, Chronic Exercise
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 30 participants (actual)
- Lead sponsor
- Inonu UniversityUniversity, hospital or other
- Study start
- 5 May 2026 (actual)
- Primary completion
- 30 Jun 2026 (actual)
- Study completion
- 30 Jun 2026 (actual)
- First posted
- 17 Sep 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This interventional study aims to investigate the effects of both acute and eight-week CrossFit training on immune system responses in sedentary adults. Participants will undergo an acute CrossFit exercise session, followed by an eight-week supervised CrossFit training program. Blood samples will be collected before and after the acute exercise session and again following the eight-week intervention to evaluate changes in selected immune system biomarkers. The primary objective is to determine whether acute and chronic CrossFit training induces significant alterations in immune function in previously sedentary individuals. The findings are expected to contribute to a better understanding of the immunological adaptations associated with high-intensity functional training and to provide evidence regarding the safety and potential health benefits of CrossFit exercise in sedentary populations.
Detailed description
CrossFit is a high-intensity functional training (HIFT) program that combines aerobic exercise, resistance training, weightlifting, gymnastics, and metabolic conditioning within a single exercise session. Owing to its increasing popularity among both athletes and the general population, there is growing interest in understanding the physiological and immunological adaptations associated with CrossFit training. Although regular physical activity is known to enhance immune function and improve overall health, high-intensity exercise may induce transient alterations in immune responses, particularly in previously sedentary individuals.
The immune system plays a critical role in protecting the body against infections and maintaining physiological homeostasis. Acute bouts of strenuous exercise may temporarily modify immune cell function and inflammatory responses, whereas long-term exercise training may promote beneficial adaptations and improve immune regulation. However, the effects of CrossFit training on immune system biomarkers in sedentary adults remain insufficiently investigated.
The purpose of this interventional study is to evaluate the effects of both an acute CrossFit exercise session and an eight-week supervised CrossFit training program on immune system responses in sedentary adults. Participants will complete baseline assessments followed by an acute CrossFit exercise session. Subsequently, they will participate in an eight-week supervised CrossFit training program. Blood samples will be collected at predetermined time points before and after the acute exercise session and following completion of the eight-week intervention to assess changes in immune system biomarkers.
The primary objective is to determine whether acute and chronic CrossFit training produce significant changes in immune function in sedentary individuals. The results of this study are expected to improve understanding of the immunological responses to high-intensity functional training and provide evidence regarding the safety and potential health benefits of CrossFit exercise for previously sedentary adults.
Study design
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Number of arms
- 1
- Model description
- All participants were assigned to a single intervention group and completed an acute CrossFit exercise session followed by an eight-week supervised CrossFit training program. Immune system biomarkers were assessed at baseline, immediately after the acute exercise session, and after completion of the eight-week intervention to evaluate acute and chronic immunological responses.
| Arm | Type | Receives |
|---|---|---|
| CrossFit Training | Experimental | Behavioral: CrossFit Training ProgramParticipants assigned to the CrossFit Training Group will participate in a structured CrossFit exercise program for 8 weeks. Training sessions will be performed three times per week under the supervision of certified trainers. Each session will include warm-up, functional strength exercises, high-intensity metabolic conditioning exercises, and cool-down periods. Exercise intensity will be monitored throughout the intervention period. |
Who may qualify
- Ages
- 19 to 24 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (13 listed)
- Currently enrolled as a student at Yalova University
- Not regularly participating in exercise or sports training
- Medically eligible to participate in CrossFit training
- Willing and able to complete the eight-week CrossFit training program conducted three days per week
- Willing to provide venous blood samples at the specified assessment times
Key exclusion criteria (0 listed)
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| CrossFit Training ProgramAlso: 8-Week CrossFit Training Programarticipants in the experimental group will participate in an eight-week CrossFit training program performed three days per week (Monday, Wednesday, and Friday). Each training session will last approximately 60 minutes and will include a dynamic warm-up, strength training exercises, a Workout of the Day (WOD) section, and a cool-down period. The strength training component will include functional resistance exercises such as squats, deadlifts, and push presses, performed for 3-5 sets of 5-10 repetitions. Training intensity will progressively increase from approximately 60-70% of one-repetition maximum (1RM) during the first four weeks to 70-80% of 1RM during the final four weeks. The WOD component will include high-intensity functional exercises such as burpees, kettlebell swings, box jumps, rowing, sit-ups, pull-ups, and short-distance running. WOD sessions will be performed using AMRAP (As Many Rounds As Possible) and For Time formats with durations of approximately 10-20 minutes. P | Behavioural | CrossFit Training |
Primary outcome
Mean Change in Total Leukocyte Count From Baseline to Completion of the Eight-Week CrossFit Program
Time frame: Baseline and immediately after completion of the eight-week CrossFit training program
Total leukocyte count will be determined from venous blood samples collected before the first training session and immediately after completion of the eight-week CrossFit training program. For each participant, the change will be calculated as the post-program value minus the baseline value. The group-level outcome will be summarized using the mean change and standard deviation.
Mean Change in Absolute Monocyte Count From Baseline to Completion of the Eight-Week CrossFit Program
Time frame: Baseline and immediately after completion of the eight-week CrossFit training program
Absolute monocyte count will be determined from venous blood samples collected before the first training session and immediately after completion of the eight-week CrossFit training program. For each participant, the change will be calculated as the post-program value minus the baseline value. The group-level outcome will be summarized using the mean change and standard deviation.
Mean Change in Absolute Lymphocyte Count From Baseline to Completion of the Eight-Week CrossFit Program
Time frame: Baseline and immediately after completion of the eight-week CrossFit training program
Absolute lymphocyte count will be determined from venous blood samples collected before the first training session and immediately after completion of the eight-week CrossFit training program. For each participant, the change will be calculated as the post-program value minus the baseline value. The group-level outcome will be summarized using the mean change and standard deviation.
Mean Change in Absolute Neutrophil Count From Baseline to Completion of the Eight-Week CrossFit Program
Time frame: Baseline and immediately after completion of the eight-week CrossFit training program
Absolute neutrophil count will be determined from venous blood samples collected before the first training session and immediately after completion of the eight-week CrossFit training program. For each participant, the change will be calculated as the post-program value minus the baseline value. The group-level outcome will be summarized using the mean change and standard deviation.
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 17 Sep 2026.
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing CrossFit Training Program in people with Immune Function Sedentary Lifestyle, run by Inonu University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 13 inclusion and 0 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 17 Sep 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Mean Change in Total Leukocyte Count From Baseline to Completion of the Eight-Week CrossFit Program (time frame: Baseline and immediately after completion of the eight-week CrossFit training program). The study lists 4 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 17 Sep 2026.