Exercise PhysiologyABP-SoccerNCT07826598
Blood Parameter Changes Following Aerobic Exercise in Professional Soccer Players
A study testing 20-Meter Shuttle Run Test in people with Exercise Physiology and Blood Parameters, run by Inonu University at 1 site in Turkey (Türkiye).
Trial summary
This study aims to investigate acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) levels following aerobic exercise in professional soccer players. Twenty professional male soccer players will voluntarily participate in the study. Read more about this study
- Who is it for?
- Ages 18 to 25 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Exercise Physiology, Blood Parameters
- What is being tested?
- 20-Meter Shuttle Run Test
- Type of treatment
- Lifestyle or therapy programme
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 32 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 25 Oct 2026to 5 Nov 2026
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 25 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Willing and able to participate in the study procedures.
- Provision of informed consent.
- Exclusion Criteria:- Inability to complete the aerobic exercise protocol.
- Withdrawal from the study or unwillingness to continue participation.
Who cannot join
None listed.
Trial locations and contacts
1 site in 1 country. Contact the study team or a site near you to ask about taking part.
musa şahin, PhD contact
Turkey (Türkiye)1 site
Exercise Physiology Laboratory, Faculty of Sport Sciences, Karabuk University
Karabük
Musa ŞAHİN, PhD, +905549755058, musasahin@karabuk.edu.tr
About this study
Official title: Aerobic Exercise-Induced Changes in Ferritin, Folate, Vitamin B12, and Parathyroid Hormone Levels in Professional Soccer Players
This study aims to investigate acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) levels following aerobic exercise in professional soccer players. Twenty professional male soccer players will voluntarily participate in the study. Participants will complete a progressive 20-meter shuttle run test. Venous blood samples will be collected immediately before and after the exercise protocol. Pre- to post-exercise changes in each blood parameter will be analyzed to determine the acute physiological responses to aerobic exercise in professional soccer players.
Read the detailed description
This single-group interventional study is designed to examine acute changes in selected blood biomarkers following aerobic exercise in professional soccer players. A total of 20 professional male soccer players aged 18-25 years will voluntarily participate. Participants will be recruited from a professional soccer club in Karabuk, Türkiye. Before participation, all individuals will receive information about the study procedures, provide written informed consent, and undergo medical screening to determine their eligibility for maximal aerobic exercise.
Body height and body mass will be measured before exercise testing. Aerobic exercise will be performed using a progressive 20-meter shuttle run test. Participants will run continuously between two lines positioned 20 meters apart while following prerecorded audio signals. Running speed will progressively increase as the interval between the audio signals decreases. The test will end when a participant fails to reach the designated line before the audio signal on two consecutive occasions.
Venous blood samples of approximately 5 mL will be collected immediately before and immediately after the exercise protocol by qualified healthcare personnel. Blood samples will be collected into anticoagulant-free serum tubes. Following a clotting period of approximately 30 minutes, the samples will be centrifuged at 3,000 revolutions per minute to separate the serum. Serum samples will be stored at -70°C until laboratory analysis.
Serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) concentrations will be measured. Pre- to post-exercise changes in each biomarker will be analyzed separately to determine the acute physiological responses elicited by aerobic exercise in professional soccer players.
- Conditions
- Exercise Physiology, Blood Parameters
- Being tested
- 20-Meter Shuttle Run Test
- Keywords
- Aerobic Exercise, Professional Soccer Players, Blood Parameters, Exercise Physiology, Acute Exercise
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
20-Meter Shuttle Run TestBehavioural
Participants will perform a progressive 20-meter shuttle run test by running continuously between two lines positioned 20 meters apart in time with prerecorded audio signals. Running speed will progressively increase as the interval between the audio signals decreases. The test will end when the participant fails to reach the designated line before the signal on two consecutive occasions.
| Study group | Role | What they receive |
|---|---|---|
| Aerobic Exercise | Experimental | All participants will complete a progressive 20-meter shuttle run test. Venous blood samples will be collected immediately before and immediately after the exercise protocol to assess acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone concentrations. Receives: Behavioral: 20-Meter Shuttle Run Test |
What is being measured
The primary outcome is the main question the study is designed to answer.
Change in Serum Ferritin Concentration From Pre-Exercise to Immediately Post-Exercise
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum ferritin concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Measured: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Change in Serum Folate Concentration From Pre-Exercise to Immediately Post-Exercise
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum folate concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Measured: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Change in Serum Vitamin B12 Concentration From Pre-Exercise to Immediately Post-Exercise
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum vitamin B12 concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Measured: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Change in Serum Parathyroid Hormone Concentration From Pre-Exercise to Immediately Post-Exercise
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum parathyroid hormone (PTH) concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Measured: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted17 Sep 2026
- Study start25 Oct 2026 (estimated)
- Primary completion29 Oct 2026 (estimated)
- Study completion5 Nov 2026 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Exercise PhysiologyStudies of the same condition, open and under way.
- More trials run by Inonu UniversityThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Model description
- All participants will be assigned to a single intervention group and will complete the same aerobic exercise protocol. Blood parameters will be assessed in relation to the exercise intervention using a within-participant design.
- Masking
- None (open label)
- Enrolment
- 32 participants (estimated)
Sponsor and investigators
- Lead sponsor
- Inonu University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Assoc. Prof. Dr. Özgür EKEN, Inonu University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07826598
- Sponsor's protocol ID
- KBÜ-AEROBIC-BLOOD-2026-01
- Acronym
- ABP-Soccer
- Status last verified
- Oct 2026
- First submitted
- 29 Aug 2026
- Last updated
- 27 Oct 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No: Individual participant data (IPD) will not be shared.
Study at a glance
From the registry record, last updated by the sponsor on 27 Oct 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Aerobic Exercise-Induced Changes in Ferritin, Folate, Vitamin B12, and Parathyroid Hormone Levels in Professional Soccer Players
- ClinicalTrials.gov ID
- NCT07826598
- Acronym / sponsor ID
- ABP-Soccer / KBÜ-AEROBIC-BLOOD-2026-01
- Conditions
- Exercise Physiology, Blood Parameters
- Keywords
- Aerobic Exercise, Professional Soccer Players, Blood Parameters, Exercise Physiology, Acute Exercise
- Study type
- Interventional
- Recruitment status
- Not yet recruiting
- Enrolment
- 32 participants (estimated)
- Lead sponsor
- Inonu UniversityUniversity, hospital or other
- Study start
- 25 Oct 2026 (estimated)
- Primary completion
- 29 Oct 2026 (estimated)
- Study completion
- 5 Nov 2026 (estimated)
- First posted
- 17 Sep 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This study aims to investigate acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) levels following aerobic exercise in professional soccer players. Twenty professional male soccer players will voluntarily participate in the study. Participants will complete a progressive 20-meter shuttle run test. Venous blood samples will be collected immediately before and after the exercise protocol. Pre- to post-exercise changes in each blood parameter will be analyzed to determine the acute physiological responses to aerobic exercise in professional soccer players.
Detailed description
This single-group interventional study is designed to examine acute changes in selected blood biomarkers following aerobic exercise in professional soccer players. A total of 20 professional male soccer players aged 18-25 years will voluntarily participate. Participants will be recruited from a professional soccer club in Karabuk, Türkiye. Before participation, all individuals will receive information about the study procedures, provide written informed consent, and undergo medical screening to determine their eligibility for maximal aerobic exercise.
Body height and body mass will be measured before exercise testing. Aerobic exercise will be performed using a progressive 20-meter shuttle run test. Participants will run continuously between two lines positioned 20 meters apart while following prerecorded audio signals. Running speed will progressively increase as the interval between the audio signals decreases. The test will end when a participant fails to reach the designated line before the audio signal on two consecutive occasions.
Venous blood samples of approximately 5 mL will be collected immediately before and immediately after the exercise protocol by qualified healthcare personnel. Blood samples will be collected into anticoagulant-free serum tubes. Following a clotting period of approximately 30 minutes, the samples will be centrifuged at 3,000 revolutions per minute to separate the serum. Serum samples will be stored at -70°C until laboratory analysis.
Serum ferritin, folate, vitamin B12, and parathyroid hormone (PTH) concentrations will be measured. Pre- to post-exercise changes in each biomarker will be analyzed separately to determine the acute physiological responses elicited by aerobic exercise in professional soccer players.
Study design
- Primary purpose
- Basic science
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Number of arms
- 1
- Model description
- All participants will be assigned to a single intervention group and will complete the same aerobic exercise protocol. Blood parameters will be assessed in relation to the exercise intervention using a within-participant design.
| Arm | Type | Receives |
|---|---|---|
| Aerobic Exercise | Experimental | Behavioral: 20-Meter Shuttle Run TestAll participants will complete a progressive 20-meter shuttle run test. Venous blood samples will be collected immediately before and immediately after the exercise protocol to assess acute changes in serum ferritin, folate, vitamin B12, and parathyroid hormone concentrations. |
Who may qualify
- Ages
- 18 to 25 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (4 listed)
- Willing and able to participate in the study procedures.
- Provision of informed consent.
- Exclusion Criteria:- Inability to complete the aerobic exercise protocol.
- Withdrawal from the study or unwillingness to continue participation.
Key exclusion criteria (0 listed)
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| 20-Meter Shuttle Run TestParticipants will perform a progressive 20-meter shuttle run test by running continuously between two lines positioned 20 meters apart in time with prerecorded audio signals. Running speed will progressively increase as the interval between the audio signals decreases. The test will end when the participant fails to reach the designated line before the signal on two consecutive occasions. | Behavioural | Aerobic Exercise |
Primary outcome
Change in Serum Ferritin Concentration From Pre-Exercise to Immediately Post-Exercise
Time frame: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum ferritin concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Change in Serum Folate Concentration From Pre-Exercise to Immediately Post-Exercise
Time frame: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum folate concentration will be measured in ng/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Change in Serum Vitamin B12 Concentration From Pre-Exercise to Immediately Post-Exercise
Time frame: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum vitamin B12 concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Change in Serum Parathyroid Hormone Concentration From Pre-Exercise to Immediately Post-Exercise
Time frame: Immediately before exercise and immediately after completion of the 20-meter shuttle run test
Venous blood samples will be collected immediately before and immediately after the 20-meter shuttle run test. Serum parathyroid hormone (PTH) concentration will be measured in pg/mL. For each participant, the change will be calculated by subtracting the pre-exercise value from the post-exercise value. The group-level outcome will be summarized using the mean change and standard deviation.
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 27 Oct 2026.
- Individual participant dataSharing planned: No. Individual participant data (IPD) will not be shared.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part.
musa şahin, PhD contact
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing 20-Meter Shuttle Run Test in people with Exercise Physiology and Blood Parameters, run by Inonu University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 4 inclusion and 0 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: opening soon, as of the sponsor's last update on 27 Oct 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
Contact Özgür EKEN, PhD (+905532132795, ozgur.eken@inonu.edu.tr), or a site near the patient. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Change in Serum Ferritin Concentration From Pre-Exercise to Immediately Post-Exercise (time frame: Immediately before exercise and immediately after completion of the 20-meter shuttle run test). The study lists 4 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 27 Oct 2026.