AML (Acute Myeloid Leukemia)Phase 1/2Dual CARCIKNCT07877610
CARCIK CD123/33 for Refractory/Relapsed AML and BPDCN in Children and Adults
A Phase 1/2 study testing CARCIK CD123/33 infusion in people with AML (Acute Myeloid Leukemia) and BPDCN, run by Fondazione Matilde Tettamanti Menotti De Marchi Onlus at 2 sites in Italy.
Trial summary
The purpose of this phase I/II clinical trial is to test CARCIK-CD123/33 cells to treat patients with R/R AML/BPDCN, who have otherwise exhausted conventional treatment options. In particular, this study will assess the safety and feasibility of CARCIK-CD123/33 cells infusion in adults and children with R/R AML, ahead… Read more about this study
- Who is it for?
- Ages 6 months to 75 years
- Conditions the study is for
- AML (Acute Myeloid Leukemia), BPDCN
- What is being tested?
- CARCIK CD123/33 infusion
- Type of treatment
- Injection or infusion
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 18 participants
- Where
- 2 sitesItaly
- Study dates
- Oct 2026to Oct 2029
- Phase
- Phase 1/2
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 6 months to 75 years
- All sexes
- No healthy volunteers
- Child, Adult, Older adult
Who may be able to join
- Patients/ parents or legal representatives able and willing to provide written informed consent and to comply with the study protocol according to ICH and local regulations. Age-appropriate assent from children will also be requested.
- Age limits: children (6 months - 17 years old) and adults (≥18 years old - 75 years old)
- Subjects with active AML/BPDCN with evidence of tumor involvement in the bone marrow and/or peripheral blood as determined by morphology and flow cytometric analysis and for whom no curative treatment option exists using currently available therapies. Specifically:
- AML/ BPDCN that has not achieved a CR, CRh, CRi, MLFS ELN criteria (1); partial remission or refractory disease (including primary refractory) after two or more lines of therapy are eligible. Or:
- AML/ BPDCN relapsed following alloHCT (including AML evolved from myelodysplastic or myeloproliferative syndrome). Note: morphologic relapse is not required; persistent/recurrent disease-associated molecular phenotypic or cytogenetic abnormalities (measurable residual disease, MRD) with measurable disease in flow cytometry > 1%, at any time after alloHCT is eligible.
- Availability of an at least haploidentical (i.e. 4/8 HLA matched by allele typing) familial or unrelated donor willing to and eligible for blood or lymphocyte donation for the CARCIKCD123/33 cell production
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- Availability of a HCT donor, including a UCB, and patient eligible to a subsequent alloHCT
- Confirmed expression of CD123 and CD33 on leukemic blasts by flow cytometry.
- Prior treatment with anti-CD123- or anti-CD33-targeted therapy is permitted, provided a minimum washout period of 2 weeks has been completed before enrollment.
- Eastern Cooperative Oncology Group (ECOG) performance status equal to 2 or less for subject ≥ 16 years of age, ore Lansky >50 for subjects < 16 years of age
- Satisfactory organ functions:
- Adequate cardiac and pulmonary function: ejection fraction (EF) by ECHO or MUGA >40%
- Adequate liver function: total bilirubin ≤ 2.0x ULN (≤ 3x ULN in patients with Gilbert's syndrome or documented liver involvement) and AST (aspartate aminotransferase)/ALT (alanine aminotransferase) 5x ULN.
- Adequate renal function: creatinine ≤1.5x ULN or creatinine clearance (CrCl) calculated by Cockroft-Gault formula of ≥50 mL/min for adult patients in whom, in the Investigator's judgment, serum creatinine levels do not adequately reflect renal function. For children, (<18 years) adequate renal function will be calculated using bedside Schwartz eGFR formula (eGFR > 60 ml/min/1.73m2).
- Life expectancy (in the opinion of the Investigator) of > 12 weeks
- Females of childbearing potential (FCBP) subjects must:
- Have a negative pregnancy test as verified by the local investigator
- Either commit to true abstinence from heterosexual contact or agree to use, and be able to comply with, effective contraception without interruption from screening until at least 12 months following CARCIKCD123/33 infusion and until CAR positive viable T cells are no longer present by quantitative polymerase chain reaction (qPCR) on two consecutive tests.
- Contraception methods must include one highly effective method, like: surgical female sterilization, use of oral (estrogen and progesterone), injected, or implanted hormonal methods of contraception or placement of an intrauterine device (IUD) or intrauterine system (IUS), or other forms of hormonal contraception that have comparable efficacy (failure rate <1%)
- Agree to abstain from breastfeeding during study participation and for at least 12 months following V infusion and until CAR positive viable T cells are no longer present by quantitative polymerase chain reaction (qPCR) on two consecutive tests.
- Male subjects must:
- Practice true abstinence or agree to use a condom during sexual contact with a pregnant female or a female of childbearing potential for 12 months after following CARCIKCD123/33 infusion and until CAR positive viable T cells are no longer present by quantitative polymerase chain reaction (qPCR) on two consecutive tests.
Who cannot join
- Patients with clinically significant active viral, bacterial or fungal infection.
- Patients with an active infection with Hepatitis B. However, patients with a controlled (treated) hepatitis will be allowed if the all the following criteria are met:
- Anti-viral therapy for hepatitis B virus (HBV) must be given for at least 1 month prior to time of informed consent.
- HBV viral load must be <2000 IU/mL (104 copies/mL) prior to time of informed consent; and
- those on active HBV therapy with viral load <2000 IU/mL (104 copies/mL) should stay on the same anti-viral therapy throughout study treatment
- Patients with an active hepatitis C virus (HCV) infection. However, patients with successfully treated chronic HCV infection will be allowed if they show a sustained virologic response at 12 weeks (SVR12) or 24 weeks (SVR24), and if there is a 4-week period between achieving sustained viral response (SVR12 or SVR24) and time of informed consent.
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- HIV Positive test (serology or PCR)
- Rapidly progressive disease that in the estimation of the investigator and sponsor could affect compliance with the protocol or interpretation of results
- Uncontrollable CNS leukemia or neurological symptoms defined as CNS grade 3. A CNS evaluation should be performed as clinically appropriated to rule out CNS involvement.
- Presence of clinically meaningful donor specific anti-HLA antibodies (DSA) against CARCIK-CD123/33 donor haplotype HLA typing
- Patients with another active invasive malignancy. Only patients with completely resected carcinoma in situ not requiring treatment other than surgery can be considered for treatment.
- Class III/IV cardiovascular disability according to New York Heart Association (NYHA) classification, myocardial infarction or cerebrovascular accident within 30 days prior to Dual CARCIK infusion, unstable arrhythmias, or unstable angina)
- Evidence of significant, uncontrolled concomitant diseases that could affect compliance with the protocol or interpretation of results, including diabetes mellitus, history of relevant pulmonary disorders (bronchospasm, obstructive pulmonary disease) and known autoimmune diseases
- Treatment with any other standard anti-cancer radiotherapy/chemotherapy (except hydroxyurea,) including investigational therapy (defined as treatment for which there is currently no regulatory authority approved indication) within 2 weeks prior to Dual CARCIK infusion
- Treatment with investigational therapy (defined as treatment for which there is currently no regulatory authority approved indication) 14 days prior to lymphodepletion or five half-life duration of the drug, or whichever comes first
- Toxicities from prior anti-cancer therapy including immunotherapy that did not resolve to ≤ Grade 1 with the exception of alopecia, endocrinopathy managed with replacement therapy and stable vitiligo.
- Prior treatment with CAR-T cell therapy within 30 days before first study treatment administration (lymphodepletion)
- Patients with a prior history of Grade 4 or higher for CRS and ICANS after CAR-T cell therapy
- Patients with a prior history of Grade 4 or higher toxicity following anti-CD33- or anti-CD123 targeted therapy
- Allogeneic or autologous HCT within 3 months or Donor Lymphocytes (DLI) within 1 month prior to CARCIK-CD123/33 infusion.
- Active GvHD Grades II-IV or moderate/severe chronic GvHD requiring systemic immunosuppressive therapy (for patients who had previously been allotransplanted)
- Administration of a live, attenuated vaccine within 4 weeks prior to CARCIK-CD123/33 infusion.
Trial locations and contacts
2 sites in 1 country. Contact the study team or a site near you to ask about taking part.
Italy2 sites, 2 recruiting
- Not yet recruiting
ASST Papa Giovanni XXIII
Bergamo, 24107
Benedetta Rambaldi, Medicine, 039352678467, brambaldi@asst-pg23.it
Benedetta Rambaldi, Medicine
- Recruiting
Fondazione IRCCS San Gerardo dei Tintori
Monza, MB, 20900
Giorgio Ottaviano, Medicine, 039392336881, giorgioantonio.ottaviano@irccs-sangerardo.it
Giovanna Lucchini, Medicine, 039392334915, giovanna.lucchini@irccs-sangerardo.it
About this study
Official title: Phase I/II Trial to Determine Safety and Efficacy of Non Viral Allogeneic Dual CD123/33 Chimeric Antigen Receptor CIK (CARCIK CD123/33) Cells in Adult and Pediatric Patients With Relapse/Refractory Acute Myeloid Leukemia (AML) and Blastic Plasmacytoid Dendritic Cell Neoplasm (BPDCN)
The purpose of this phase I/II clinical trial is to test CARCIK-CD123/33 cells to treat patients with R/R AML/BPDCN, who have otherwise exhausted conventional treatment options. In particular, this study will assess the safety and feasibility of CARCIK-CD123/33 cells infusion in adults and children with R/R AML, ahead of scheduled alloHCT.
- Conditions
- AML (Acute Myeloid Leukemia), BPDCN
- Being tested
- CARCIK CD123/33 infusion
- Medical subject headings
- Leukemia, Myeloid, Acute, Blastic Plasmacytoid Dendritic Cell Neoplasm
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
CARCIK CD123/33 infusionDrug
split dose of CARCIK cells
| Study group | Role | What they receive |
|---|---|---|
| single arm of patients treated with CARCIK CD123/33 | Experimental | Not described Receives: Drug: CARCIK CD123/33 infusion |
What is being measured
The primary outcome is the main question the study is designed to answer.
Long term survival
Measured: 12 months from treatment
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
Oct 2026Oct 2029
- First posted16 Oct 2026
- Study startOct 2026 (estimated)
- Primary completionOct 2028 (estimated)
- Study completionOct 2029 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for AML (Acute Myeloid Leukemia)Studies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase 1/2
- Primary purpose
- Treatment
- Allocation
- Not applicable
- Intervention model
- Single group
- Masking
- None (open label)
- Enrolment
- 18 participants (estimated)
Sponsor and investigators
- Lead sponsor
- Fondazione Matilde Tettamanti Menotti De Marchi Onlus
- Sponsor type
- University, hospital or other
- Responsible party
- Sponsor
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07877610
- Sponsor's protocol ID
- FT05 CARCIK
- Other IDs
- 2025 524977 18 00, 2025
- Acronym
- Dual CARCIK
- Status last verified
- Oct 2026
- First submitted
- 8 Oct 2026
- Last updated
- 16 Oct 2026
- Results posted
- No
- Data monitoring committee
- Yes
- FDA-regulated drug
- No
- FDA-regulated device
- No