Reduce Immunosuppression With Atmp in NS ChildrEn

Background:

Nephrotic Syndrome (NS) is a rare disease characterized by nephrotic-range proteinuria and the need for steroid treatment. About 50% of children will become frequent relapsers (FRNS) or steroid dependent (SDNS), requiring higher doses of steroids or other immunosuppressive drugs for many years, sometimes up to adulthood. Strong evidence suggests that Idiopathic nephrotic syndrome (INS), at least in the steroid-sensitive forms, has an immune pathogenesis.

Mesenchymal Stromal Cells (MSC) are multipotent non-hematopoietic stem cells that produce an immunomodulatory activity in-vitro and in-vivo. For this reason, we postulated that SDNS patients could benefit with treatment with MSC.

Objectives:

The main goal of the present study is to assess whether CB-MSCs have the capacity to regulate the immunologic mechanisms involved in the pathogenesis of NS allowing for a reduction or suspension of chronic immunosuppressive treatment in SDNS children. The primary objective is to evaluate whether CB-MSC therapy is able to prevent NS recurrence for at least 6 months after complete withdrawal of immunosuppressive treatment in children with SDNS.

Primary endpoint: percentage of children without NS recurrence after complete withdrawal of immunosuppressive treatment for at least 6 months.

Population:

We plan to enroll 11 children (3 to 18 years of age) with SDNS, maintained by chronic immunosuppressive treatment and with stable remission for at least two months.

Study Design:

Open label single-arm, monocentric trial, with a rescue/second design

Phase: Phase II

Inclusion/Exclusion Criteria:

Inclusion criteria

1. Age between 3 and 18 years;
2. Clinical diagnosis of SDNS;
3. Disease remission maintained by chronic therapy (at least 6 months) with either:

• Use of a combination of 2 or more immunosuppressive drugs
• use of 1 of the calcineurin inhibitors (Cyclosporin or Tacrolimus);

1. Absence of proteinuria (urinary protein:urinary creatinine < 0.2 mg/mg) for at least 1 month;
2. Estimated glomerular filtration rate greater than or equal to 70 ml/min/1.73 m^2;
3. Written informed consent from parents or guardians and the child when possible.

Exclusion criteria

1. Age < 3 years or ≥ 19 years;
2. Resistant/refractory NS;
3. Presence of genetic mutations associated with NS;
4. eGFR less than 70 ml/min/1.73 m^2;
5. Thrombophilic condition;
6. Pregnancy or lactating;
7. Evidence of an uncooperative attitude;
8. Any evidence that the patient will be unable to complete the trial follow-up.

Description of the Intervention:

The trial will rely on a single advanced therapy medicinal product (ATMP), made by MSC from umbilical cord blood (CB) for allogeneic use, produced following a highly standardized process, developed and controlled at Cell Factory under the Good Manufacturing Practices guidelines.

After baseline clinical and laboratory evaluation, patients will receive 3 intravenous infusions of CB-MSCs at the dosage of 1.5 x 10^6/kg at a time interval of 1 to 2 weeks.

The immunosuppressive treatment will be gradually tapered off following the first CB-MSC administration: 25%, 50% and 100% reduction of the ongoing immunosuppressive treatment following the first, second and third administration, respectively.

At the end of this first part of the trial, a statistical analysis will be performed according to the primary end-point. In the case of failure to reach the primary end point an extra 11 children with SDNS will be enrolled in a single stage phase 2 study with a 30% incremented dose of CB-MSCs.

Statistical Evaluation:

A phase II design with a rescue plan B, is adopted to decide whether the proportion responding is less than or equal to 0.200 or greater than or equal to 0.600. A sample size of 10 children is required, considering a drop out of 10%, 11 children will be enrolled: if the number of responses is 5 or more, the hypothesis that P ≤ 0.200 is rejected with a target error rate of 5%. If the number of responses is 4 or less, the hypothesis that P ≥ 0.600 is rejected with a target error rate of 20% (power equal to 80%). The same statistical hypothesis will apply for the second part of the study.

Expected Results:

The results of this proposal will provide information regarding an innovative cell therapy for the treatment of INS. If the hypothesis of this study is confirmed, it will be possible to reduce or withdraw immunosuppressive treatment in this vulnerable population of children with SDNS, reducing the need for ambulatory visits and hospitalization and therapy-related complications. Furthermore, the reduction in the use of immunosuppressive agents as well as in the number of outpatient visits and hospital admissions will reduce the financial burden to the National Health Service. Above all, the quality of life of children with SDNS would clearly be improved, in terms of a reduction in long-term therapy, morbidity and the number of visits.