Skeletal Muscle PhysiologyNCT04136457
Proteome Remodelling
A study testing Endurance training, Resistance training and Sprint training in people with Skeletal Muscle Physiology, run by Morten Hostrup, PhD at 1 site in Denmark.
Trial summary
The purpose of the project is to investigate the proteome signature dynamics and remodelling in human skeletal muscle induced by exercise training Read more about this study
- Who is it for?
- Ages 18 to 45 yearsHealthy volunteers can take part
- Conditions the study is for
- Skeletal Muscle Physiology
- What is being tested?
- Endurance training, Resistance training and Sprint training
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 72 participants
- Where
- 1 siteDenmark
- Study dates
- 30 Oct 2019to 28 Nov 2023
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 45 years
- All sexes
- Healthy volunteers accepted
- Adult
Who may be able to join
- Healthy men and women
- VO2max > 55 mL/min/kgLM for men and > 50 for women (only applicable for endurance and sprint training interventions)
- BMI < 26
Who cannot join
- Chronic disease deemed by the medical doctor to pose a risk or to affect study outcomes
- smoking
- Use of prescription medicine deemed by the medical to affect study outcomes
- Pregnancy
Trial locations and contacts
1 site in 1 country.
About this study
Official title: Proteome Remodelling and Dynamics in Human Skeletal Muscle: Effect of Training
The purpose of the project is to investigate the proteome signature dynamics and remodelling in human skeletal muscle induced by exercise training
- Conditions
- Skeletal Muscle Physiology
- Being tested
- Endurance training, Resistance training, Sprint training
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Endurance trainingOther
Subjects perform a period of endurance training
Resistance trainingOther
Subjects perform a period of resistance training
Sprint trainingOther
Subjects perform a period of sprint training
| Study group | Role | What they receive |
|---|---|---|
| Endurance | Experimental | Endurance training Receives: Other: Endurance training |
| Resistance | Experimental | Resistance training Receives: Other: Resistance training |
| Sprint | Experimental | Sprint training Receives: Other: Sprint training |
What is being measured
The primary outcome is the main question the study is designed to answer.
Skeletal muscle proteome signature
Pathway enrichment analysis of the muscle proteome
Measured: Change from baseline at week 6 or 8 depending on the intervention
Anabolic signaling
Western blotting of proteins related to anabolic signaling
Measured: Change in anabolic signaling from rest to 1 hour following resistance exercise both at baseline and following 8 weeks of resistance training
Secondary outcomes (6)
Skeletal muscle mitochondrial respiration
High resolution respirometry
Measured: Change from baseline at week 6 following endurance and sprint training
Maximal oxygen consumption
VO2max during incremental exercise
Measured: Change from baseline at week 6 following endurance and sprint training
Body composition
Lean and fat mass during dual X-ray absorptiometry
Measured: Change from baseline at week 6 or 8 depending on the intervention
Myonuclei
Immunohistochemistry to determine number of myonuclei
Measured: Change from baseline at week 8 following resistance training
Satellite cells
Immunohistochemistry to determine number of satellite cells
Measured: Change from baseline at week 8 following resistance training
Fiber size
Immunohistochemistry to determine fiber size
Measured: Change from baseline at week 8 following resistance training
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted23 Oct 2019
- Study start30 Oct 2019
- Primary completion28 Nov 2023
- Study completion28 Nov 2023
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Publications (1)Papers the sponsor has linked to this study.
- Other trials for Skeletal Muscle PhysiologyStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Enrolment
- 72 participants (actual)
Sponsor and investigators
- Lead sponsor
- Morten Hostrup, PhD
- Sponsor type
- University, hospital or other
- Responsible party
- Sponsor-investigator, Morten Hostrup, PhD, University of Copenhagen
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT04136457
- Sponsor's protocol ID
- MiDy
- Status last verified
- Dec 2023
- First submitted
- 21 Oct 2019
- Last updated
- 27 Dec 2023
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- Undecided
Publications
Kissow J, Jacobsen KJ, Jessen S, Thomsen LB, Quesada JP, Bangsbo J, Deshmukh AS, Hostrup M. Global Proteomics Reveals Distinct Muscle Adaptations to Menstrual Cycle Phase-Based Sprint Interval Training in Endurance-Trained Females. Mol Cell Proteomics. 2025 Oct;24(10):101053. doi: 10.1016/j.mcpro.2025.101053. Epub 2025 Aug 14.
PubMed 40818533
Study at a glance
From the registry record, last updated by the sponsor on 27 Dec 2023. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Proteome Remodelling and Dynamics in Human Skeletal Muscle: Effect of Training
- ClinicalTrials.gov ID
- NCT04136457
- Acronym / sponsor ID
- MiDy
- Conditions
- Skeletal Muscle Physiology
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 72 participants (actual)
- Lead sponsor
- Morten Hostrup, PhDUniversity, hospital or other
- Study start
- 30 Oct 2019 (actual)
- Primary completion
- 28 Nov 2023 (actual)
- Study completion
- 28 Nov 2023 (actual)
- First posted
- 23 Oct 2019 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The purpose of the project is to investigate the proteome signature dynamics and remodelling in human skeletal muscle induced by exercise training
Study design
- Primary purpose
- Basic science
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Number of arms
- 3
| Arm | Type | Receives |
|---|---|---|
| Endurance | Experimental | Other: Endurance trainingEndurance training |
| Resistance | Experimental | Other: Resistance trainingResistance training |
| Sprint | Experimental | Other: Sprint trainingSprint training |
Who may qualify
- Ages
- 18 to 45 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (3 listed)
- Healthy men and women
- VO2max > 55 mL/min/kgLM for men and > 50 for women (only applicable for endurance and sprint training interventions)
- BMI < 26
Key exclusion criteria (4 listed)
- Chronic disease deemed by the medical doctor to pose a risk or to affect study outcomes
- smoking
- Use of prescription medicine deemed by the medical to affect study outcomes
- Pregnancy
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Endurance trainingSubjects perform a period of endurance training | Other | Endurance |
| Resistance trainingSubjects perform a period of resistance training | Other | Resistance |
| Sprint trainingSubjects perform a period of sprint training | Other | Sprint |
Primary outcome
Skeletal muscle proteome signature
Time frame: Change from baseline at week 6 or 8 depending on the intervention
Pathway enrichment analysis of the muscle proteome
Anabolic signaling
Time frame: Change in anabolic signaling from rest to 1 hour following resistance exercise both at baseline and following 8 weeks of resistance training
Western blotting of proteins related to anabolic signaling
6 secondary outcomes
Skeletal muscle mitochondrial respiration
Time frame: Change from baseline at week 6 following endurance and sprint training
Maximal oxygen consumption
Time frame: Change from baseline at week 6 following endurance and sprint training
Body composition
Time frame: Change from baseline at week 6 or 8 depending on the intervention
Myonuclei
Time frame: Change from baseline at week 8 following resistance training
Satellite cells
Time frame: Change from baseline at week 8 following resistance training
Fiber size
Time frame: Change from baseline at week 8 following resistance training
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 27 Dec 2023.
- Derived from this study: PubMed 40818533Kissow J, Jacobsen KJ, Jessen S, Thomsen LB, Quesada JP, Bangsbo J, Deshmukh AS, Hostrup M. Global Proteomics Reveals Distinct Muscle Adaptations to Menstrual Cycle Phase-Based Sprint Interval Training in Endurance-Trained Females. Mol Cell Proteomics. 2025 Oct;24(10):101053. doi: 10.1016/j.mcpro.2025.101053. Epub 2025 Aug 14.
- Individual participant dataSharing planned: Undecided
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Endurance training, Resistance training and Sprint training in people with Skeletal Muscle Physiology, run by Morten Hostrup, PhD at 1 site in Denmark.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 3 inclusion and 4 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 27 Dec 2023. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Denmark. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Skeletal muscle proteome signature (time frame: Change from baseline at week 6 or 8 depending on the intervention). The study lists 2 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
On the ClinicalTrials.gov record for NCT04136457, which also lists 1 linked publication.
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 27 Dec 2023.