tDCS and Motor Learning in Children With DCD

Transcranial direct-current stimulation (tDCS) is one of the most common non-invasive brain stimulation techniques but applications in pediatric populations are relatively unexplored. tDCS applies a weak electric current over the scalp to modulate cortical excitability. Anodal stimulation excites the stimulated brain region. Anodal tDCS applied to the motor cortex contralateral to the trained hand enhances motor learning across single or multiple day training sessions (Reis 2009). tDCS has shown promising effects in the developing brain including the potential to enhance motor skills that may then be transferred to other untrained skills in typically developing children (Ciechanski 2017). tDCS is safe and tolerable and a feasible technique with only mild short-term adverse effects (e.g., redness, tingling, itching, and burning sensation) in children. The adverse effects usually occur at electrode sites and disappear within a few minutes after stimulation starts (Krishnan 2015).

Combined with motor-based interventions, tDCS can enhance motor performance in adults (Reis 2009, 2011) and children (Gillick 2014; Grecco 2017; Kirton 2017; Moura 2016). It is currently being used in combination with other treatments (e.g., behavioural therapy and neurorehabilitation) in children and adolescents with neurodevelopmental disorders (Muszkat 2016), including Autism Spectrum Disorder (Amatachaya 2014) and Attention-Deficit/Hyperactivity Disorder (ADHD) (Bandeira 2016), as well as motor disorders such as Cerebral Palsy (Gillick 2014; Grecco 2017; Kirton 2017; Moura 2016). However, the effect of tDCS on skill motor learning in children with Developmental Coordination Disorder (DCD) is largely unexplored.

DCD is a chronic motor disorder of unknown etiology that affects 5-6% of school-aged children in Canada (APA, 2013). DCD interferes with children's academic achievement and limits their ability to participate in daily activities (e.g., printing, getting dressed, tying their shoes, riding a bike, using cutlery), as well as vocational activities, leisure, and play (APA, 2013).15 Subsequently, children may develop psychosocial difficulties, including low self-esteem, depression, anxiety, loneliness, problems with peers, and poor participation in physical and social activities (Zwicker 2013). DCD is a lifelong condition, and 75% of children with DCD will continue to experience motor difficulties as adults if they don't receive proper treatment (Kirby 2014). Up to half of children with DCD also have co-occurring ADHD (Piek 1999).

Several brain regions have been implicated in DCD, including the cerebellum, basal ganglia, parietal lobe, and parts of the frontal lobe (e.g., dorsolateral prefrontal cortex or DLPFC) (Biotteau 2016). The primary motor cortex (M1) is located in the dorsal part of the frontal lobe and is functionally connected to other motor areas. However, the functional connectivity between M1 and brain regions involved in motor functioning and sensorimotor processing, such as striatum and angular gyrus, may be decreased in children with DCD (McLeod 2014). Targeting such specific brain regions in rehabilitation might be effective in improving the motor outcomes of affected children.

Currently, the most beneficial interventions to improve the motor performance of children with DCD are task-oriented approaches that focus on learning a particular task rather than on the body functions required to perform a task (Smits-Engelsman 2013). A number of task-oriented approaches are commonly used to treat children with DCD (Niemeijer 2007; Polatajko 2001). The investigators believe that brain stimulation can enhance motor learning and the effect of task-oriented approaches in children with DCD. To better understand tDCS as a treatment for children with DCD, as the first step, it is critical to investigate whether tDCS can enhance motor skill learning in this population. Therefore, the investigators aim to conduct a randomized, blinded, sham-controlled interventional trial of anodal tDCS over M1 combined with a motor learning task to assess its effectiveness on motor skill learning in children with DCD. This is a pilot study to determine a sample size for a larger study.

AIMS AND HYPOTHESES

Aim 1: To determine if transcranial direct current stimulation (tDCS) enhances motor learning in children with DCD.

Hypothesis 1: Compared to children in sham group, children in stimulation group will show better functional outcomes faster motor learning in each session (online learning) and after 3 sessions.

Aim 2: To determine the longevity of tDCS effects on motor learning in children with DCD.

Hypothesis 2: Children in stimulation group will maintain their motor learning after 6 weeks compared to the sham group.

METHODOLOGY

Study Design: This study is a randomized, sham-controlled, double-blinded trial. Participants will be randomly assigned to active or sham stimulation.

Participants: Children will be recruited from established cohorts of children with DCD who were assessed at BC Children's Hospital or Sunny Hill Health Centre for Children (Vancouver, BC) and who meet DSM-5 diagnostic criteria (American Psychiatric Association, 2013).

Sample size: Sample size was calculated based on a randomized sham-control designed study in typically-developing children receiving M1 A-tDCS or sham tDCS over 3 consecutive days of Purdue Pegboard Test training.4 Sample size calculation suggested a total of 14 subjects, 7 subjects per group, would have a power of 95% to detect improvement in Purdue Pegboard Test (effect size = 2.58) with a type-1 error of 0.05.

Procedure: After screening and recruitment, parents will consent and children will assent to take part in the study. We will randomize children to active or sham stimulation groups; a statistician will randomize participants using computer-generated sequential blocks of 4 to 6. Randomization codes will be kept in sealed opaque envelopes until study enrollment. A research graduate student with training in occupational therapy will be blinded to group assignments and will assess children using Purdue Pegboard Test (PPT; Tiffin 1968), Bruininks-Oseretsky Test of Motor Proficiency-2 (BOT-2; Bruininks 2005), and Evaluation Tool of Children's Handwriting (ETCH; Amundson, 1995). Then, children will receive 3 days of tDCS for 30 minutes each day; during the first 10 minutes, children will complete Purdue Pegboard Test Training (to assess learning of a motor task), followed by 20 minutes of handwriting practice using "Printing Like a Pro!"(Montgomery 2017), to assess learning of a functional motor task). An occupational therapist will re-assess the children at the end of the last day of training and again 6 weeks later.

Interventions

tDCS: Direct current will be delivered using a transcranial electrical stimulator approved by Health Canada (Soterix Medical Inc., New York, USA) (Soterix Medical, 2016). Stimulation will be applied to the scalp through two 5×7 cm sponge saline-soaked electrodes: active and reference. A simple headgear system, including the EASYpads and EASYstraps, will hold the electrodes in place. In the active stimulation and sham groups, the anode (active electrode) will be positioned over the left primary motor cortex with the cathode (reference electrode) over right forehead in supraorbital area. The international 10/20 electroencephalography electrode system will be used to localize the M1 (Klem 1999). The dominant left motor cortex will be stimulated because the investigators aim to simultaneously train the dominant hand for a motor learning task and a functional task. The stimulation will be applied at 1 mA for 30 min. One mA of anodal stimulation may cause brain current densities in children on average comparable to densities seen in adults exposed to 2 mA current (Kessler 2013) and the subsequent excitability might last longer than one hour (Moliadze 2015). For active stimulation groups, the current will be ramped up to 1 mA over 45-60 s, held for 30 min, and ramped down to 0 mA over 45-60 s. For the sham groups, stimulation will be ramped up and held for only 60 s before it is slowly ramped down. This procedure, called the Fade-in-Short Stimulation-Fade out, has shown its reliability as an effective sham technique through making the same tolerability and transient scalp sensation as active stimulation in both adults (Ambrus 2012) and children (Ciechanski 2017). In case of any "Serious Adverse Events" (e.g., second-degree scalp burn at the site of electrode pad, or clinical seizure) occurring during the course of study, it will be stopped immediately.

Motor Learning Task: Over three consecutive days, each child will perform five blocks of Purdue Pegboard Test: one block before, three blocks during, and one block after tDCS. Each block consists of three repetitions of Purdue Pegboard Test with the right hand. The children have to place pins into a pegboard as fast as they can in 30 seconds. It will take up to 10 minutes of brain stimulation time.

Functional Motor Task: After the Purdue Pegboard Test, each child will receive cognitive-based intervention for printing skills for 20 minutes while receiving tDCS. "Printing Like a Pro!" (Montgomery 2017) -a cognitive approach to teaching printing to primary school-age children-will be used to teach letters which each child has the most difficulty printing legibly as identified on a formal assessment of handwriting-ETCH (manuscript) (Amundson 1995).

Data Analysis Plan:

Purdue Pegboard Test is the primary outcome measure of interest. To measure online learning within one session and off-line learning across sessions, we will apply Repeated Measure Analysis of Co-variance (ANCOVA), with an α level of 0.05. To measure effect of intervention and retention, we will apply a paired t-test and Repeated Measure ANCOVA to the primary and secondary outcomes. Two-way ANCOVA and independent t-test will also be used to compare groups (stimulation versus sham). MABC-2 scores and attention level as assessed by Conner's ADHD Index will be used as covariates to account for individual differences in attention and motor skills.

Significance:

This is the first study of its kind to both investigate the effect of brain stimulation in motor learning in children with DCD and integrate technology to improve functional motor learning for children with DCD. This study will contribute to planning more effective interventions for these children to improve both their motor skills and functional outcomes. Additionally, findings will be of interest to pediatric clinicians (e.g., occupational therapists) and parents seeking more efficient approaches for these children, as well as researchers, students, and policy makers in the field of neurorehabilitation.