RiLuzole to Reduce Atrial FIb Study Using Holter Monitoring

HYPOTHESIS: Riluzole will statistically reduce the number of AF episodes compared to placebo over a one-month follow-up period in patients with permanent AF (PAF).

A prospective, double-blind, randomized, placebo-controlled, two-arm study design in patients with PAF will be conducted to determine efficacy by:

1. Number of episodes tachycardia in 1 month between riluzole 50mg BID versus matching placebo;
2. Time to first episode of tachycardia between riluzole 50mg BID and placebo; and
3. Safety (pro-arrhythmia, neutropenia by CBC [baseline and 1-month end] and patient signs and symptoms [weekly phone call F/U]).

SPECIFIC AIMS:

AIM 1: To determine the impact of riluzole versus placebo based on quantification of number of episodes of tachycardia between riluzole and placebo over 1 month follow-up. A second analysis of the time to first episode of tachycardia between riluzole and placebo.

AIM 2: To determine the safety of riluzole versus placebo during a 1-month administration period. Safety will be determined by appearance of pro-arrhythmia, neutropenia by CBC [baseline and 1 month end] and patient signs and symptoms [weekly phone call F/U]) compared to placebo.

Dosing of Riluzole and Placebo: Riluzole 50mg BID (at awakening and early evening) with matching placebo tablets.

STUDY DESIGN:

Double-Blind Study Protocol

The study will be conducted in accordance with the Declaration of Helsinki and have received approval from the University of Utah Institutional Review Board.

Study Population Seventy-eight adult patients with a history of symptomatic AF documented electrocardiographically within > 48 hours to 12 months will be recruited to participate in this trial. An additional 12 patients will be recruited (90 total), if necessary, for patients that are unable to complete the 30-day follow-up period. Patients will be excluded if they have a Systolic BP > 180 mmHg or Diastolic BP > 100 mmHg; atrial Fibrillation due to electrolyte imbalance, hyperthyroidism, pericarditis, or other reversible illness; NYHA FC IV Heart Failure (No ADHF Decompensation with 1 month); unstable angina, AMI, coronary surgery within 3 or coronary angioplasty within 1 month of screening; Wolff-Parkinson-White syndrome unless treated with successful ablation; infiltrative heart disease; severe valvular heart disease; history of syncope or angina precipitated by an ventricular arrhythmia, torsade de pointes, polymorphic ventricular tachycardia, or sustained monomorphic ventricular tachycardia, or cardia arrest; are prescribed VW Class I or III antiarrhythmic agents, potent CYP1A2 inhibitors; who are of child-bearing age not practicing study-defined protective measures; or are active smokers.

At screening, each patient will be have the protocol explained to them through an informed consent process, be able to ask questions, have their primary care physician / electrophysiologist review the consent form, and then make an informed decision about their participation.

For each patient that consents to participate they will have their blood drawn for a comprehensive blood chemistry-30 panel and complete blood count. If a female who is either not of childbearing potential (defined as postmenopausal for at least 1 year or surgically sterile [bilateral tubal ligation, bilateral oophorectomy, or hysterectomy]) or is practicing 1 of the following medically acceptable methods of birth control for at least one full menstrual cycle prior to screening (listed under Exclusion Criteria #13), and agrees to continue with the regimen from the time of Screening, throughout the entire study, they will be have a pregnancy test prior to initiating drug testing.

The study investigators will determine eligibility of each participant prior to randomization. If the patient is eligible to participate, a random number generated table will select study treatment: riluzole or placebo.

After each patient starts the study medication, the patient will be scheduled to undergo a cardioversion from a clinical standpoint for atrial fibrillation management. The cardioversion can be scheduled any time after the patient has been on the medication for 3 days. At the time of the cardioversion, ECG will confirm conversion to normal sinus rhythm, before the patient starts taking study medication.

During the 30-day follow-up period, heart rhythm will be monitored by continuous heart rhythm monitoring to assess AF burden. The event recorder (monitored cardiac telemetry or event monitor or holter monitor) automatically recorded atrial fibrillation based on irregularity in the R-R interval, an established method for the detection of atrial fibrillation, 6 over a period of 30 beats at any rate. Recorders will be attached to a dry-electrode (nonadhesive) belt worn around the chest (Cardiac Bio-Systems) to enable better compliance by the patients with prolonged monitoring than has been typically observed with conventional adhesive skin-contact electrodes. Patients will be instructed to wear the monitor as much as possible for 30 days. Recorded ECG data will be transmitted transtelephonically for central interpretation. All the episodes of atrial fibrillation will be adjudicated by a cardiologist who is unaware of the patient's demographic and clinical characteristics.

Throughout the 30-day study period, the patient will have access to study investigators through a 24-hour phone number to contact the study site for any reason. At day seven each patient will receive a phone call from the study site to determine return of atrial fibrillation symptoms and any study medication adverse effects.

At the end of the 30-period treatment period, each participant will return to the clinic to have their blood drawn for a comprehensive blood chemistry-30 panel and complete blood count.

Open-Label Extension Protocol

At the end of the study, all patients will become unblinded to study treatment. If the patient has no return of atrial fibrillation during the 30-day study period and is tolerating riluzole, the patient will be offered to continue treatment for an additional 90-day without charge for riluzole. During this open-label extension phase each patient will have continuous 14-day phone call follow-up by the study investigators to determine any atrial fibrillation symptoms and continued tolerance of the riluzole medication. At the end of the 90-day open-label extension period the participant will be referred to their primary physician for continued atrial fibrillation care.

STATISTICAL ANALYSIS:

Across all the analyses for the baseline characteristics, efficacy outcome and safety outcomes, p-value less than 0.05 from statistical test will be considered significant.

Baseline Characteristics Baseline characteristics of all patients in enrolled in the study will be collected. (Gender, Age, Primary Indication for Implant, CVA/TIA history, concomitant diseases/disorders, medications). Patient characteristics will be compared between the Riluzole and Placebo groups using Chi-square test and Student t-test for categorical and continuous variables, respectively.

Outcome Analysis:

Primary Efficacy Outcome - Number of tachycardia episodes per week observation. The primary outcome is the number of tachycardia episodes over treatment periods by modified intention-to-treat (at least 14 days with tachycardia data obtained). To adjust for the potential difference in the observation period attributable to any incomplete follow-up, the number of tachycardia will be divided by the seven-day interval to calculate the event density, per-patient per-week (PPPW) number of tachycardia episodes. The PPPW will be compared between the Riluzole and Placebo using a Generalized Linear Model (GLM) with a Poisson distribution and log-link function.

Secondary outcome - Time to first tachycardia event The secondary outcome of this study will be time to first tachycardia event (nth day) after the administration of either Riluzole or placebo. The cumulative proportion of patients having tachycardia by follow-up period (# days) will be calculated using Kaplan-Meier product-limit estimate where patients will be censored on the outcome date, end of 30-day follow up period, discontinuation of the assigned treatment, or loss-of-follow up for any reason including patient death. Using a Cox-proportional hazard regression model, hazard ratio for the Riluzole vs. Placebo will be calculated as a measure of treatment effect

Adverse Events. The proportion of patients reporting any adverse experience will be compared between treatment groups using Chi-square test for the two binomial probabilities. This analysis will include all patients, not just those used in the primary efficacy analysis. Adverse experiences for each patient will be reviewed. Patients who discontinued because of a noncardiac adverse experience will be recorded. Patients who discontinued because of inadequate response or continued will be recorded separately.

Statistical power and sample size. The study is aimed to determine the superiority of the Riluzole compared to the Placebo with the target incidence density ratio (primary outcome) and hazard ratio (secondary outcome) of 0.5. (Edward et al.) with α = 0.05 and β=0.8.

The minimal sample size to detect the incidence density ratio of 0.5 from a Generalized Linear Model with Poisson distribution and log-link, this study will need a total of 78 subjects. To determine the hazard ratio of 0.5 with the overall event rate of 50% assuming that a third of the riluzole and two-third of the placebo group have at least one tachycardia during the follow-up, the total sample size must be greater than or equal to 104. Thus 55 patients randomly allocated to each group will be sufficiently powered and allows nominal attrition (<> 5%). Sample size estimation was performed using STATA 13.0 (College Station, TX: StataCorp LP).