Dietary FiberFiberPlayNCT05726435
Effects of Soluble Dietary Fiber on Sport Efficiency and Fatigue Delay in Top Basketball Players
A study testing Soluble dietary fiber with prebiotic properties in people with Dietary Fiber and Sports, run by University of Zagreb at 1 site in Croatia.
Trial summary
Athlete nutrition is becoming an increasingly important factor on the path to achieving top results. Nutrients such as soluble fiber can alter the intestinal microbiota, resulting in metabolites with a positive effect on metabolism. Read more about this study
- Who is it for?
- Ages 18 to 22 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Dietary Fiber, Sports, Sports Nutrition
- What is being tested?
- Soluble dietary fiber with prebiotic properties
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 20 participants
- Where
- 1 siteCroatia
- Study dates
- 1 Sep 2021to 20 Dec 2021
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 22 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Professional basketball players from the same team participating in the same monitored training process
Who cannot join
- Diabetes or other chronic disease, taking sports performance enhancing drugs, taking antibiotics 3 months prior or during the course of the study
Trial locations and contacts
1 site in 1 country.
About this study
Athlete nutrition is becoming an increasingly important factor on the path to achieving top results. Nutrients such as soluble fiber can alter the intestinal microbiota, resulting in metabolites with a positive effect on metabolism. Thus, the fibers become available, an additional source of energy to the host, have an anti-stress effect and delay the feeling of fatigue. The primary goal of the study is to determine the impact of soluble dietary fiber on increasing athletic efficiency, the secondary goal is to determine the impact of fiber on delaying fatigue. Subjects will be randomly divided into experimental and control group. The sample of variables will consist of genome microbiota genome sequencing data, measures to assess morphological characteristics, lower extremity explosive strength tests, and cardiovascular endurance. The statistical package SPSS will be used for data processing. The expected scientific contribution of this research is to expand the scientific knowledge about the importance of dietary fiber in the diet of athletes with the aim of improving sports performance.
Read the detailed description
Material, participants, methodology and research plan The material will be soluble fiber NUTRIOSE and placebo. Nutriose, derived from corn, is generally well tolerated and no allergic reactions are known. Participants will be made aware of this in informed consent. If, however, there will be any adverse reactions in participant, participant will withdraw from the study immediately.
The participants will be 14 adult, professional basketball players. The sample of subjects was determined by the size of the group in the same training process in order to exclude the influence of different training processes on the research results. Participants who have used antibiotics in the last 3 months, participants with type 1 and 2 diabetes, and also who have used other ergogenic aids in the last 30 days and still using them are excluded.
Participants will be randomly divided into two groups: the experimental group that will consume the tested fibers and the control group that will consume placebo. Design is a double-blind study where an independent statistician will be entrusted with the randomization of subjects into the two groups.
Sample variables:
Methods: dietary methods, anthropometric methods, fatigue assessment questionnaire and fecal samples analysis.
The sample of variables consists of: fecal sample for intestinal microbiota sequencing, measures to assess morphological characteristics (body height, body weight, triceps skinfold, subscapular skinfold, chest skinfold, abdomen skinfold, suprailiocristal skinfold, thigh skinfold, axillary skinfold, fat percentage calculated by the seven skinfolds algorithm).
Dietary methods will be used to control eating habits and fiber intake prior to testing and to control diet during testing. The methods will be a food diary (three-day) and as an unannounced control of daily food intake once a week will be a 24-hour dietary recalls. The three-day food diary will show diet and average fiber intake just before the start of testing and unannounced control of daily food intake as evidence of unchanged eating habits during the study. All collected dietary data will be processed using Croatian food tables in addition to American tables for all foods whose chemical composition is not available in national tables.
In assessing the athletic performance of basketball players, emphasis will be placed on the explosive power of the lower extremities (concentric and eccentric contractions). Anthropometric methods will use tests to assess vertical explosive power (squat jump, countermovement jump, one legged countermovement jump, maximal countermovement jump, continuous jumps with straight egs, continuous jump with bent legs) with Optojump system and explosive power - sprint (5m, 10m and 20m sprint) with the Powertimer Newtest system. Aerobic endurance tests (BEEP test with sound signal) and anaerobic endurance test (300 m (15x20)) with Powertimer Newtest system will be used to assess cardiovascular endurance. Laboratory measurements will be performed within the Sports Diagnostic Center of the Faculty of Kinesiology, University of Zagreb.
Fecal samples will be collected at the Faculty of Food Technology and Biotechnology, University of Zagreb. Analysis of the composition of the intestinal microbiota by DNA sequencing from fecal samples will be performed using an Illumina NGS device.
Participants will complete a questionnaire on a daily basis in subjective feeling of fatigue on a scale of 1 to 10 (rate of perceived exertion).
Participants will have a daily short questionnaire on: bloating, change in stool consistency, nausea and weakness.
Research plan:
The research lasts a total of 8 weeks. Initial measurements before the start of supplementation include anthropometric testing and taking the first microbiota sample. This is followed by taking another microbiota sample 2 weeks after the start of supplementation. Final measurements follow at the end of 4 weeks of supplementation that include anthropometric testing and taking a third microbiota sample. Finally, the fourth microbiota sample will be 4 weeks after the final and 8 weeks after the initial measurements, respectively. After getting acquainted with the basics of testing, participants will sign a consent to participate.
Three days before the initial testing, participants will be explained how collect feces and the collection containers will be distributed. Each participant will be subjected to a dietary method of measuring food intake, a three-day diet diary and unannounced controls of daily food intake, once a week. Initial measurements will begin by collecting fecal-sampled containers. After measuring the morphological characteristics, tests to assess explosive power and aerobic and anaerobic capacities will be approached. After the initial testing, during the regular competition season, the participants of both groups will continue the training process without the possibility of additional training or changes in the training process. The experimental group (n = 7) will consume 2x8.5 g/day of soluble dietary fiber over four weeks, while the control group (n = 7) will consume the same amount of placebo carbohydrates. The dose was determined by previous studies with a proven effect on the morphological characteristics of participants and without side effects.
Participants will be taken by the respondents after the training in the presence of the research leader, and on the day off they will send a picture of an empty bag in mobile phone application. This will resolve "compliance." The training process will be the same for both groups, and participants will be advised not to change their diet pattern for two weeks prior to the test as well as for the duration of the experiment. After each training session and basketball game, respondents will complete a questionnaire in subjective feeling of fatigue on a scale of 1 to 10 (rate of perceived exertion). The final measurements are equal to the initial ones.
Data processing methods:
The sample size in the study was estimated through a test power of 70 % and a significance level of 5 %. The statistical program SPSS (Statistical Package for the Social Science), version 17.0., SPSS, Inc. will be used for data processing. Descriptive statistical parameters of the monitored variables will be calculated: arithmetic mean (AS), standard deviation (SD), minimum (MIN), maximum (MAX) and range result (RAS) and curvature (KURT) and distribution asymmetry (SKEW), and normality distribution by the Kolmogorov-Smirnov test. Multivariate and univariate analysis of variance (MANOVA and ANOVA) will be used to analyze the effects of training between initial and final measurements within groups, as well as to analyze differences in effects between groups. In case of statistically significant differences between groups, Tukey post hoc analysis will be used to determine the difference between specific groups. All conclusions will be drawn at the level of statistical error p <0.05.
- Conditions
- Dietary Fiber, Sports, Sports Nutrition, Microbiota, Nutrition Surveys
- Being tested
- Soluble dietary fiber with prebiotic properties, Placebo
- Keywords
- Fiber, Gastrointestinal Microbiota, Nutrition, 16S rRNA sequencing, Performance
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Soluble dietary fiber with prebiotic propertiesDietary supplement
Also called Nutriose
14 adult professional baskeyball players will be divided randomly in two groups, each containing 7 players. One group shall receive Nutriose fiber, two times per day 10 grams per dose in total duration of 4 weeks. Second group shall receive same amount of Placebo in form of Maltodextrin. Each group will undergo a series of sports performance tests and questionnaires and shall donate their feces several times during the study in order to monitor gut microbiota changes. The Nutriose group in this particular intervention contains 7 randomy selected players.
PlaceboDietary supplement
Also called Maltodextrin
14 adult professional baskeyball players will be divided randomly in two groups, each containing 7 players. One group shall receive Nutriose fiber, two times per day 10 grams per dose in total duration of 4 weeks. Second group shall receive same amount of Placebo in form of Maltodextrin. Each group will undergo a series of sports performance tests and questionnaires and shall donate their feces several times during the study in order to monitor gut microbiota changes. This second (Placebo) group also consists of the 7 players and receives Maltodextrin supplementation. Since the study type is "double-blind", neither the participants (players) nor the person handing out the supplementation know the exact nature of fiber substance being handed during the entire duration of the trial. Independent statistician is handling the randomization of both interventions.
| Study group | Role | What they receive |
|---|---|---|
| Experimental group | Active comparator | Seven randomly selected active basketball players will be supplemented with defined prebiotic fibre (20 grams per day divided in two 10 gram doses - 85% total fiber content) for the total duration of 4 weeks, while being stationed in the training camp under constant coach and nutrition expert surveillance. Receives: Dietary Supplement: Soluble dietary fiber with prebiotic properties |
| Control group | Placebo comparator | Seven randomly selected active basketball players will be supplemented with placebo resembling prebiotic fibre in all visible and taste aspects (20 grams per day divided in two 10 gram doses) for the total duration of 4 weeks, while being stationed in the training camp under constant coach and nutrition expert surveillance. Receives: Dietary Supplement: Placebo |
What is being measured
The primary outcome is the main question the study is designed to answer.
Rapid microbiota change
The impact of fiber supplementation on gut microbiome shall assessed through monitoring of relative taxonomic abundance of bacteria that compose gut microbiota. DNA shall be extracted from collected faecal samples, stored at -80°C. DNA extraction shall be performed using the QIAamp® PowerFecal® DNA Kit. DNA concentration shall be quantified using Promega Quantus Flourometer before being sent to amplicon sequencing of variable regions 3 and 4 of the 16S rRNA gene with the primer set 341F (5'-CCTAYGGGRBGCASCAG -3') 806R (5'- GGACTACNNGGGTATCTAAT -3'). Obtained sequenceing raw data (reads) shall be analyzed using 'Quantitative Insights Into Microbial Ecology 2' (QIIME2) software in order to evaluate microbial composition of each sample. The comparison on intial sample (before intervention) and sample 1 (after 2 weeks) of supplementation shall provide a baseline insight into eventual rapid microbiota change as a response to supplementation/placebo.
Measured: 2 weeks
Altered microbiota composition
Prolonged supplementation after initial 2 weeks will support the established altered gut microbiota in participants receiving fiber supplement. The impact of fiber supplementation on gut microbiome shall assessed through monitoring of relative taxonomic abundance of bacteria that compose gut microbiota. Gut microbiota DNA shall be extracted from collected faecal samples, stored at -80°C. DNA shall be processed in the same manner as previously described (Outcome 1). Obtained sequenceing raw data (reads) shall be analyzed using 'Quantitative Insights Into Microbial Ecology 2' (QIIME2) software in order to evaluate microbial composition of each sample. The comparison on intial sample (before intervention) and sample 2 (after 4 weeks) of supplementation shall provide an insight into eventual microbiota change as a response to prolonged supplementation/placebo.
Measured: 4 weeks
Effect on the perception of fatigue
The major question is whether the moderately increasing fiber intake in athletes has the potential to support the intestinal microbiota and conversely positively impact training-induced perception of fatigue. In order to understand the impact of fiber consumption on the perception of fatigue, after each training session or match, the participants shall be asked to record and send a rating of perceived exertion through a mobile messaging application. The Borg's CR10 RPE scale shall be used for this purpose. This scale has a range from 0 to 10 (RPE - Rating of Perceived Exertion). The RPE scale is a tool used to monitor the perceptual response to training, as a method of determining physical exertion during exercise. The original RPE scale, developed more than 40 years ago, was primarily used to monitor aerobic load, but it still remains widely used in athlete tracking.
Measured: 4 weeks
Secondary outcomes (1)
Long-term microbiota impact
After total of 8 weeks fiber supplementation, one additional microbiota composition measurement (16S rRNA sequencing of donated fecal samples after 8 weeks of trial) will be taken after additional 4 weeks with no supplementation (intervention), in order to determine if the initial microbiota change caused by fiber supplementation can persist without additional fiber supplementation and to assess the potential for a long-term induced microbiota change. The comparison on intial sample (before intervention), sample 1 (after 2 weeks) of supplementation and sample 2 (after 4 weeks) with sample 3 (after 8 weeks) shall provide a baseline insight into microbiota change as a response to supplementation/placebo. DNA extraction and data processing shall be the same as in previous outcomes.
Measured: 8 weeks
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted14 Feb 2023
- Study start1 Sep 2021
- Primary completion31 Oct 2021
- Study completion20 Dec 2021
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Publications (11)Papers the sponsor has linked to this study.
- Other trials for Dietary FiberStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Model description
- Double-blind study of defined prebiotic supplementation for active basketball players investigating fiber effect on microbiota composition and delayed feeling of fatigue. There are two arms, active one receiving prebiotic fibre and control one receiving placebo. Each arm contains 7 randomly selected participants who signed informed consent and will receive supplementation during 4 weeks in total while being in the same training camp and under same training conditions.
- Masking
- Double blind
- Who is masked
- Investigator, Outcomes assessor
- Enrolment
- 20 participants (actual)
Sponsor and investigators
- Lead sponsor
- University of Zagreb
- Sponsor type
- University, hospital or other
- Collaborators
- Croatian Science Foundation
- Responsible party
- Principal investigator, Antonio Starcevic, University of Zagreb
- Investigators
- Antonio Starcevic, PhD, Principal investigator, University of Zagreb, Faculty of Food Technology and Biotechnology
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT05726435
- Sponsor's protocol ID
- IP-2016-06-3509
- Acronym
- FiberPlay
- Status last verified
- Feb 2023
- First submitted
- 13 Jul 2021
- Last updated
- 14 Feb 2023
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Publications
Wosinska L, Cotter PD, O'Sullivan O, Guinane C. The Potential Impact of Probiotics on the Gut Microbiome of Athletes. Nutrients. 2019 Sep 21;11(10):2270. doi: 10.3390/nu11102270.
PubMed 31546638Marttinen M, Ala-Jaakkola R, Laitila A, Lehtinen MJ. Gut Microbiota, Probiotics and Physical Performance in Athletes and Physically Active Individuals. Nutrients. 2020 Sep 25;12(10):2936. doi: 10.3390/nu12102936.
PubMed 32992765Clark A, Mach N. Exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes. J Int Soc Sports Nutr. 2016 Nov 24;13:43. doi: 10.1186/s12970-016-0155-6. eCollection 2016.
PubMed 27924137Mohr AE, Jager R, Carpenter KC, Kerksick CM, Purpura M, Townsend JR, West NP, Black K, Gleeson M, Pyne DB, Wells SD, Arent SM, Kreider RB, Campbell BI, Bannock L, Scheiman J, Wissent CJ, Pane M, Kalman DS, Pugh JN, Ortega-Santos CP, Ter Haar JA, Arciero PJ, Antonio J. The athletic gut microbiota. J Int Soc Sports Nutr. 2020 May 12;17(1):24. doi: 10.1186/s12970-020-00353-w.
PubMed 32398103Erdogan Z, Erdogan S, Aslantas O, Celik S. Effects of dietary supplementation of synbiotics and phytobiotics on performance, caecal coliform population and some oxidant/antioxidant parameters of broilers. J Anim Physiol Anim Nutr (Berl). 2010 Oct;94(5):e40-8. doi: 10.1111/j.1439-0396.2009.00973.x.
PubMed 20487099Lefranc-Millot C, Guerin-Deremaux L, Wils D, Neut C, Miller LE, Saniez-Degrave MH. Impact of a resistant dextrin on intestinal ecology: how altering the digestive ecosystem with NUTRIOSE(R), a soluble fibre with prebiotic properties, may be beneficial for health. J Int Med Res. 2012;40(1):211-24. doi: 10.1177/147323001204000122.
PubMed 22429361Farhangi MA, Javid AZ, Sarmadi B, Karimi P, Dehghan P. A randomized controlled trial on the efficacy of resistant dextrin, as functional food, in women with type 2 diabetes: Targeting the hypothalamic-pituitary-adrenal axis and immune system. Clin Nutr. 2018 Aug;37(4):1216-1223. doi: 10.1016/j.clnu.2017.06.005. Epub 2017 Jun 10.
PubMed 28669666Aliasgharzadeh A, Dehghan P, Gargari BP, Asghari-Jafarabadi M. Resistant dextrin, as a prebiotic, improves insulin resistance and inflammation in women with type 2 diabetes: a randomised controlled clinical trial. Br J Nutr. 2015 Jan 28;113(2):321-30. doi: 10.1017/S0007114514003675.
PubMed 27028002Li S, Guerin-Deremaux L, Pochat M, Wils D, Reifer C, Miller LE. NUTRIOSE dietary fiber supplementation improves insulin resistance and determinants of metabolic syndrome in overweight men: a double-blind, randomized, placebo-controlled study. Appl Physiol Nutr Metab. 2010 Dec;35(6):773-82. doi: 10.1139/H10-074.
PubMed 21164548Guerin-Deremaux L, Pochat M, Reifer C, Wils D, Cho S, Miller LE. The soluble fiber NUTRIOSE induces a dose-dependent beneficial impact on satiety over time in humans. Nutr Res. 2011 Sep;31(9):665-72. doi: 10.1016/j.nutres.2011.09.004.
PubMed 22024490Nazare JA, Sauvinet V, Normand S, Guerin-Deremaux L, Gabert L, Desage M, Wils D, Laville M. Impact of a resistant dextrin with a prolonged oxidation pattern on day-long ghrelin profile. J Am Coll Nutr. 2011 Feb;30(1):63-72. doi: 10.1080/07315724.2011.10719945.
PubMed 21697540
Study at a glance
From the registry record, last updated by the sponsor on 14 Feb 2023. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Effects of Soluble Dietary Fiber on Sport Efficiency and Fatigue Delay in Top Basketball Players
- ClinicalTrials.gov ID
- NCT05726435
- Acronym / sponsor ID
- FiberPlay / IP-2016-06-3509
- Conditions
- Dietary Fiber, Sports, Sports Nutrition, Microbiota, Nutrition Surveys
- Keywords
- Fiber, Gastrointestinal Microbiota, Nutrition, 16S rRNA sequencing, Performance
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 20 participants (actual)
- Lead sponsor
- University of ZagrebUniversity, hospital or other
- Collaborators
- Croatian Science Foundation
- Study start
- 1 Sep 2021 (actual)
- Primary completion
- 31 Oct 2021 (actual)
- Study completion
- 20 Dec 2021 (actual)
- First posted
- 14 Feb 2023 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Athlete nutrition is becoming an increasingly important factor on the path to achieving top results. Nutrients such as soluble fiber can alter the intestinal microbiota, resulting in metabolites with a positive effect on metabolism. Thus, the fibers become available, an additional source of energy to the host, have an anti-stress effect and delay the feeling of fatigue. The primary goal of the study is to determine the impact of soluble dietary fiber on increasing athletic efficiency, the secondary goal is to determine the impact of fiber on delaying fatigue. Subjects will be randomly divided into experimental and control group. The sample of variables will consist of genome microbiota genome sequencing data, measures to assess morphological characteristics, lower extremity explosive strength tests, and cardiovascular endurance. The statistical package SPSS will be used for data processing. The expected scientific contribution of this research is to expand the scientific knowledge about the importance of dietary fiber in the diet of athletes with the aim of improving sports performance.
Detailed description
Material, participants, methodology and research plan The material will be soluble fiber NUTRIOSE and placebo. Nutriose, derived from corn, is generally well tolerated and no allergic reactions are known. Participants will be made aware of this in informed consent. If, however, there will be any adverse reactions in participant, participant will withdraw from the study immediately.
The participants will be 14 adult, professional basketball players. The sample of subjects was determined by the size of the group in the same training process in order to exclude the influence of different training processes on the research results. Participants who have used antibiotics in the last 3 months, participants with type 1 and 2 diabetes, and also who have used other ergogenic aids in the last 30 days and still using them are excluded.
Participants will be randomly divided into two groups: the experimental group that will consume the tested fibers and the control group that will consume placebo. Design is a double-blind study where an independent statistician will be entrusted with the randomization of subjects into the two groups.
Sample variables:
Methods: dietary methods, anthropometric methods, fatigue assessment questionnaire and fecal samples analysis.
The sample of variables consists of: fecal sample for intestinal microbiota sequencing, measures to assess morphological characteristics (body height, body weight, triceps skinfold, subscapular skinfold, chest skinfold, abdomen skinfold, suprailiocristal skinfold, thigh skinfold, axillary skinfold, fat percentage calculated by the seven skinfolds algorithm).
Dietary methods will be used to control eating habits and fiber intake prior to testing and to control diet during testing. The methods will be a food diary (three-day) and as an unannounced control of daily food intake once a week will be a 24-hour dietary recalls. The three-day food diary will show diet and average fiber intake just before the start of testing and unannounced control of daily food intake as evidence of unchanged eating habits during the study. All collected dietary data will be processed using Croatian food tables in addition to American tables for all foods whose chemical composition is not available in national tables.
In assessing the athletic performance of basketball players, emphasis will be placed on the explosive power of the lower extremities (concentric and eccentric contractions). Anthropometric methods will use tests to assess vertical explosive power (squat jump, countermovement jump, one legged countermovement jump, maximal countermovement jump, continuous jumps with straight egs, continuous jump with bent legs) with Optojump system and explosive power - sprint (5m, 10m and 20m sprint) with the Powertimer Newtest system. Aerobic endurance tests (BEEP test with sound signal) and anaerobic endurance test (300 m (15x20)) with Powertimer Newtest system will be used to assess cardiovascular endurance. Laboratory measurements will be performed within the Sports Diagnostic Center of the Faculty of Kinesiology, University of Zagreb.
Fecal samples will be collected at the Faculty of Food Technology and Biotechnology, University of Zagreb. Analysis of the composition of the intestinal microbiota by DNA sequencing from fecal samples will be performed using an Illumina NGS device.
Participants will complete a questionnaire on a daily basis in subjective feeling of fatigue on a scale of 1 to 10 (rate of perceived exertion).
Participants will have a daily short questionnaire on: bloating, change in stool consistency, nausea and weakness.
Research plan:
The research lasts a total of 8 weeks. Initial measurements before the start of supplementation include anthropometric testing and taking the first microbiota sample. This is followed by taking another microbiota sample 2 weeks after the start of supplementation. Final measurements follow at the end of 4 weeks of supplementation that include anthropometric testing and taking a third microbiota sample. Finally, the fourth microbiota sample will be 4 weeks after the final and 8 weeks after the initial measurements, respectively. After getting acquainted with the basics of testing, participants will sign a consent to participate.
Three days before the initial testing, participants will be explained how collect feces and the collection containers will be distributed. Each participant will be subjected to a dietary method of measuring food intake, a three-day diet diary and unannounced controls of daily food intake, once a week. Initial measurements will begin by collecting fecal-sampled containers. After measuring the morphological characteristics, tests to assess explosive power and aerobic and anaerobic capacities will be approached. After the initial testing, during the regular competition season, the participants of both groups will continue the training process without the possibility of additional training or changes in the training process. The experimental group (n = 7) will consume 2x8.5 g/day of soluble dietary fiber over four weeks, while the control group (n = 7) will consume the same amount of placebo carbohydrates. The dose was determined by previous studies with a proven effect on the morphological characteristics of participants and without side effects.
Participants will be taken by the respondents after the training in the presence of the research leader, and on the day off they will send a picture of an empty bag in mobile phone application. This will resolve "compliance." The training process will be the same for both groups, and participants will be advised not to change their diet pattern for two weeks prior to the test as well as for the duration of the experiment. After each training session and basketball game, respondents will complete a questionnaire in subjective feeling of fatigue on a scale of 1 to 10 (rate of perceived exertion). The final measurements are equal to the initial ones.
Data processing methods:
The sample size in the study was estimated through a test power of 70 % and a significance level of 5 %. The statistical program SPSS (Statistical Package for the Social Science), version 17.0., SPSS, Inc. will be used for data processing. Descriptive statistical parameters of the monitored variables will be calculated: arithmetic mean (AS), standard deviation (SD), minimum (MIN), maximum (MAX) and range result (RAS) and curvature (KURT) and distribution asymmetry (SKEW), and normality distribution by the Kolmogorov-Smirnov test. Multivariate and univariate analysis of variance (MANOVA and ANOVA) will be used to analyze the effects of training between initial and final measurements within groups, as well as to analyze differences in effects between groups. In case of statistically significant differences between groups, Tukey post hoc analysis will be used to determine the difference between specific groups. All conclusions will be drawn at the level of statistical error p <0.05.
Study design
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Double blind (investigator, outcomes assessor)
- Number of arms
- 2
- Model description
- Double-blind study of defined prebiotic supplementation for active basketball players investigating fiber effect on microbiota composition and delayed feeling of fatigue. There are two arms, active one receiving prebiotic fibre and control one receiving placebo. Each arm contains 7 randomly selected participants who signed informed consent and will receive supplementation during 4 weeks in total while being in the same training camp and under same training conditions.
| Arm | Type | Receives |
|---|---|---|
| Experimental group | Active comparator | Dietary Supplement: Soluble dietary fiber with prebiotic propertiesSeven randomly selected active basketball players will be supplemented with defined prebiotic fibre (20 grams per day divided in two 10 gram doses - 85% total fiber content) for the total duration of 4 weeks, while being stationed in the training camp under constant coach and nutrition expert surveillance. |
| Control group | Placebo comparator | Dietary Supplement: PlaceboSeven randomly selected active basketball players will be supplemented with placebo resembling prebiotic fibre in all visible and taste aspects (20 grams per day divided in two 10 gram doses) for the total duration of 4 weeks, while being stationed in the training camp under constant coach and nutrition expert surveillance. |
Who may qualify
- Ages
- 18 to 22 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (1 listed)
- Professional basketball players from the same team participating in the same monitored training process
Key exclusion criteria (1 listed)
- Diabetes or other chronic disease, taking sports performance enhancing drugs, taking antibiotics 3 months prior or during the course of the study
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Soluble dietary fiber with prebiotic propertiesAlso: Nutriose14 adult professional baskeyball players will be divided randomly in two groups, each containing 7 players. One group shall receive Nutriose fiber, two times per day 10 grams per dose in total duration of 4 weeks. Second group shall receive same amount of Placebo in form of Maltodextrin. Each group will undergo a series of sports performance tests and questionnaires and shall donate their feces several times during the study in order to monitor gut microbiota changes. The Nutriose group in this particular intervention contains 7 randomy selected players. | Dietary supplement | Experimental group |
| PlaceboAlso: Maltodextrin14 adult professional baskeyball players will be divided randomly in two groups, each containing 7 players. One group shall receive Nutriose fiber, two times per day 10 grams per dose in total duration of 4 weeks. Second group shall receive same amount of Placebo in form of Maltodextrin. Each group will undergo a series of sports performance tests and questionnaires and shall donate their feces several times during the study in order to monitor gut microbiota changes. This second (Placebo) group also consists of the 7 players and receives Maltodextrin supplementation. Since the study type is "double-blind", neither the participants (players) nor the person handing out the supplementation know the exact nature of fiber substance being handed during the entire duration of the trial. Independent statistician is handling the randomization of both interventions. | Dietary supplement | Control group |
Primary outcome
Rapid microbiota change
Time frame: 2 weeks
The impact of fiber supplementation on gut microbiome shall assessed through monitoring of relative taxonomic abundance of bacteria that compose gut microbiota. DNA shall be extracted from collected faecal samples, stored at -80°C. DNA extraction shall be performed using the QIAamp® PowerFecal® DNA Kit. DNA concentration shall be quantified using Promega Quantus Flourometer before being sent to amplicon sequencing of variable regions 3 and 4 of the 16S rRNA gene with the primer set 341F (5'-CCTAYGGGRBGCASCAG -3') 806R (5'- GGACTACNNGGGTATCTAAT -3'). Obtained sequenceing raw data (reads) shall be analyzed using 'Quantitative Insights Into Microbial Ecology 2' (QIIME2) software in order to evaluate microbial composition of each sample. The comparison on intial sample (before intervention) and sample 1 (after 2 weeks) of supplementation shall provide a baseline insight into eventual rapid microbiota change as a response to supplementation/placebo.
Altered microbiota composition
Time frame: 4 weeks
Prolonged supplementation after initial 2 weeks will support the established altered gut microbiota in participants receiving fiber supplement. The impact of fiber supplementation on gut microbiome shall assessed through monitoring of relative taxonomic abundance of bacteria that compose gut microbiota. Gut microbiota DNA shall be extracted from collected faecal samples, stored at -80°C. DNA shall be processed in the same manner as previously described (Outcome 1). Obtained sequenceing raw data (reads) shall be analyzed using 'Quantitative Insights Into Microbial Ecology 2' (QIIME2) software in order to evaluate microbial composition of each sample. The comparison on intial sample (before intervention) and sample 2 (after 4 weeks) of supplementation shall provide an insight into eventual microbiota change as a response to prolonged supplementation/placebo.
Effect on the perception of fatigue
Time frame: 4 weeks
The major question is whether the moderately increasing fiber intake in athletes has the potential to support the intestinal microbiota and conversely positively impact training-induced perception of fatigue. In order to understand the impact of fiber consumption on the perception of fatigue, after each training session or match, the participants shall be asked to record and send a rating of perceived exertion through a mobile messaging application. The Borg's CR10 RPE scale shall be used for this purpose. This scale has a range from 0 to 10 (RPE - Rating of Perceived Exertion). The RPE scale is a tool used to monitor the perceptual response to training, as a method of determining physical exertion during exercise. The original RPE scale, developed more than 40 years ago, was primarily used to monitor aerobic load, but it still remains widely used in athlete tracking.
1 secondary outcome
Long-term microbiota impact
Time frame: 8 weeks
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 14 Feb 2023.
- Background: PubMed 31546638Wosinska L, Cotter PD, O'Sullivan O, Guinane C. The Potential Impact of Probiotics on the Gut Microbiome of Athletes. Nutrients. 2019 Sep 21;11(10):2270. doi: 10.3390/nu11102270.
- Background: PubMed 32992765Marttinen M, Ala-Jaakkola R, Laitila A, Lehtinen MJ. Gut Microbiota, Probiotics and Physical Performance in Athletes and Physically Active Individuals. Nutrients. 2020 Sep 25;12(10):2936. doi: 10.3390/nu12102936.
- Background: PubMed 27924137Clark A, Mach N. Exercise-induced stress behavior, gut-microbiota-brain axis and diet: a systematic review for athletes. J Int Soc Sports Nutr. 2016 Nov 24;13:43. doi: 10.1186/s12970-016-0155-6. eCollection 2016.
- Background: PubMed 32398103Mohr AE, Jager R, Carpenter KC, Kerksick CM, Purpura M, Townsend JR, West NP, Black K, Gleeson M, Pyne DB, Wells SD, Arent SM, Kreider RB, Campbell BI, Bannock L, Scheiman J, Wissent CJ, Pane M, Kalman DS, Pugh JN, Ortega-Santos CP, Ter Haar JA, Arciero PJ, Antonio J. The athletic gut microbiota. J Int Soc Sports Nutr. 2020 May 12;17(1):24. doi: 10.1186/s12970-020-00353-w.
- Background: PubMed 20487099Erdogan Z, Erdogan S, Aslantas O, Celik S. Effects of dietary supplementation of synbiotics and phytobiotics on performance, caecal coliform population and some oxidant/antioxidant parameters of broilers. J Anim Physiol Anim Nutr (Berl). 2010 Oct;94(5):e40-8. doi: 10.1111/j.1439-0396.2009.00973.x.
- Background: PubMed 22429361Lefranc-Millot C, Guerin-Deremaux L, Wils D, Neut C, Miller LE, Saniez-Degrave MH. Impact of a resistant dextrin on intestinal ecology: how altering the digestive ecosystem with NUTRIOSE(R), a soluble fibre with prebiotic properties, may be beneficial for health. J Int Med Res. 2012;40(1):211-24. doi: 10.1177/147323001204000122.
- Background: PubMed 28669666Farhangi MA, Javid AZ, Sarmadi B, Karimi P, Dehghan P. A randomized controlled trial on the efficacy of resistant dextrin, as functional food, in women with type 2 diabetes: Targeting the hypothalamic-pituitary-adrenal axis and immune system. Clin Nutr. 2018 Aug;37(4):1216-1223. doi: 10.1016/j.clnu.2017.06.005. Epub 2017 Jun 10.
- Background: PubMed 27028002Aliasgharzadeh A, Dehghan P, Gargari BP, Asghari-Jafarabadi M. Resistant dextrin, as a prebiotic, improves insulin resistance and inflammation in women with type 2 diabetes: a randomised controlled clinical trial. Br J Nutr. 2015 Jan 28;113(2):321-30. doi: 10.1017/S0007114514003675.
- Background: PubMed 21164548Li S, Guerin-Deremaux L, Pochat M, Wils D, Reifer C, Miller LE. NUTRIOSE dietary fiber supplementation improves insulin resistance and determinants of metabolic syndrome in overweight men: a double-blind, randomized, placebo-controlled study. Appl Physiol Nutr Metab. 2010 Dec;35(6):773-82. doi: 10.1139/H10-074.
- Background: PubMed 22024490Guerin-Deremaux L, Pochat M, Reifer C, Wils D, Cho S, Miller LE. The soluble fiber NUTRIOSE induces a dose-dependent beneficial impact on satiety over time in humans. Nutr Res. 2011 Sep;31(9):665-72. doi: 10.1016/j.nutres.2011.09.004.
- Background: PubMed 21697540Nazare JA, Sauvinet V, Normand S, Guerin-Deremaux L, Gabert L, Desage M, Wils D, Laville M. Impact of a resistant dextrin with a prolonged oxidation pattern on day-long ghrelin profile. J Am Coll Nutr. 2011 Feb;30(1):63-72. doi: 10.1080/07315724.2011.10719945.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Antonio Starcevic, PhD principal investigator
University of Zagreb, Faculty of Food Technology and Biotechnology
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Soluble dietary fiber with prebiotic properties in people with Dietary Fiber and Sports, run by University of Zagreb at 1 site in Croatia.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 1 inclusion and 1 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 14 Feb 2023. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Croatia. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Rapid microbiota change (time frame: 2 weeks). The study lists 3 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
On the ClinicalTrials.gov record for NCT05726435, which also lists 11 linked publications.
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 14 Feb 2023.