CreatineNCT05981820
High Intensity Interval Training, Creatine Intake and Anaerobic Power
A study testing Creatine and HIIT training in people with Creatine and High Intensity Interval Training, run by Ankara University at 1 site in Turkey (Türkiye).
Trial summary
Creatine is an ergogenic aid with positive effects on muscle and performance. The aim of the study was to determine whether there is a difference between the effects of creatine supplementation taken together with Wingate-based HIIT on anaerobic power, leg strength, heart rate variability and body composition. Read more about this study
- Who is it for?
- Ages 18 to 30 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Creatine, High Intensity Interval Training
- What is being tested?
- Creatine and HIIT training
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 15 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 10 Mar 2022to 15 Jan 2023
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 30 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- having no health and communication problem
- using no drugs or ergogenic aids
- being physically active adult men aged 18-30 years
Who cannot join
- using any drugs or ergogenic aids
- having any musculoskeletal problem
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Ankara University Spor Bilimleri Fakultesi
Gölbaşı, Ankara, 06830
About this study
Official title: Effect of High Intensity Interval Training and Creatine Intake on Anaerobic Power and Muscle Strength
Creatine is an ergogenic aid with positive effects on muscle and performance. The aim of the study was to determine whether there is a difference between the effects of creatine supplementation taken together with Wingate-based HIIT on anaerobic power, leg strength, heart rate variability and body composition. In the study, Wingate-based HIIT program was applied three days a week for six weeks. Anaerobic power (Wingate anaerobic power test), heart rate variability (Polar H10 chest band), leg strength (back and leg dynamometer) and body composition (PlusAvis 333) measurements of the participants were made 1 week before the training program started and the week after the training program ended. Twenty physically active adult men aged 18-30 were voluntarily included for the study. Participants were divided into two groups: Wingate-based HIIT with creatine supplementation (K-HIIT) and Wingate-based HIIT only (HIIT). As a result of the analysis, it was seen that creatine supplementation taken together with wingate-based HIIT had an effect and increased on the anaerobic power parameters, peak power, average power and minimum power values, and leg strength. In addition, it was determined that heart rate variability parameters improved heart rate (HR, beat/min) and low frequency (LF, ms2) values, but did not cause any change in body composition. Comparing and investigating the effects of different HIIT methods on anaerobic power, leg strength, heart rate variability and body composition will increase the generalizability of the study.
Read the detailed description
Despite the time-efficiency of HIIT in inducing chronic physiological adaptation, it depleted phosphocreatine stores in the muscle and decrease power output. It has been observed that high-intensity exercise performance increases with the increase of intramuscular phosphocreatine. It is thought that with creatine supplementation, the rate of phosphocreatine resynthesis and mitochondrial transfer of ATP to the cytoplasm may increase. This suggests that performing HIIT with creatine supplementation will provide further improvement. Therefore, the purpose of this study to compare the effectiveness of HIIT with creatine supplement compared to HIIT only. We hypothesized that creatine supplementation intake with wingate-based HIIT will positively affect power capacity and performance-related parameters.
The research will be carried out using experimental research design, one of the quantitative research methods, to measure the variables and determine the relationship between dependent and independent variables. Anaerobic power, heart rate variability, leg strength, and body composition measurements of the participants will be made 1 week before the training program started and the week after the training program ended.
The G*power software was utilized in order to determine the appropriate sample size. It has been reported that a minimum of 12 participants, 6 in each group, should be included in the study (alpha value = 0.05, 1-beta value = 0.80, and effect size = 1.6). Measurement of tests and the Wingate-based HIIT program will be conducted in the Performance Laboratory of the Faculty of Sports Sciences of Ankara University. Twenty physically active adult men aged 18-30 years, who had no history of medical and did not use drugs or ergogenic aids, will be recruited voluntarily. The ethics committee approval for the study methodology was obtained from the Ethics Committee of Ankara University Rectorate (Number: E-56786525-050.04.04/378028 Decision Number: 2022/02) and it will be carried out in compliance with the Declaration of Helsinki's principles. Written inform consents which explain the study to the participant and any right and risk will be obtained prior to data collection. Permission for the research to be carried out in the Performance Laboratory of the Faculty of Sport Sciences of Ankara University will be obtained from the Deanery of the Faculty of Sport Sciences of Ankara University. Participants will be divided into two groups as those who will perform Wingate-based HIIT with creatine (C-HIIT) (age: 21,78 ± 1,79 years; height: 179.56 ± 8.26 cm; weight: 76.86 ± 10.66 kg) and those who will perform only Wingate-based HIIT (HIIT) (age: 20.167 ± 0,98 years; height: 172.33 ± 9,88 cm ; weight: 66.53 ± 9.51kg ). Out of twenty potential participants, only 15 participants agreed to participate in the study (HIIT=6, C-HIIT=9) and 5 dropped out because of sport injuries that occured before the study process.
IBM SPSS 24.0 program (SPSS Inc., Chicago, IL, USA) will be used to statistically analyze the data obtained in the study. First of all, the distribution of the data will be evaluated to determine the test used to compare the data as parametric or non-parametric. This normality distribution assessment will be tested with Shapiro Wilk, as both groups had less than 50 participants. Determination of the mean difference in within-group statistics will be performed with Paired Sample t-Test in data with normal distribution and with Wilcoxon test in data where it is not normal. Groups will be expressed as mean ± standard error. American Psychological Association (APA) 6.0 style will be used to report statistical differences. The alpha value will be accepted as 0.05 in all statistical analysis.
This study aimed to determine whether there was a difference in the effects of taking creatine supplementation with Wingate-based HIIT on anaerobic power, leg strength, heart rate variability and body composition.
The limitation of the current research is that the study will be carried out with a single gender. In future studies on the subject, the number of participants can be increased, female participants can be included in the research and it can be examined whether there is a difference between genders and to increase the generalizability of the study, the effects of different HIIT methods on anaerobic power, leg strength, heart rate variability and body composition can be compared by investigating. In addition, the trial period may be extended as longer periods are needed to see clearer changes in HRV.
- Conditions
- Creatine, High Intensity Interval Training
- Being tested
- Creatine, HIIT training
- Keywords
- ergogenic aids, HIIT, creatine, performance
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
CreatineDietary supplement
To observe the effects of creatine in the study, 10 g of creatine supplementation was taken only on training days during the study period. Participants used 10 g of creatine monohydrate by dissolved in 200 ml of water. Creatine supplementation was taken in two doses of five grams, 30 minutes before and after training.
HIIT trainingOther
In the first three weeks:
Warm-up: Warm-up at 70 rpm for 4 minutes, including 2-3 seconds of maximal sprint at 1.30 and 2.30 minutes Wingate-based HIIT: HIIT 4 sets of 12x15 seconds of maximal repetitions with 15 seconds of passive rest between reps in each set Cool-down: 4 minutes of passive rest between sets
In the last three weeks:
Warm-up: Warm-up at 70 rpm for 4 minutes, including 2-3 seconds of maximal sprint at 1.30 and 2.30 minutes Wingate-based HIIT: HIIT 5 sets of 20x15 seconds of maximal repetitions with 15 seconds of passive rest between reps in each set Cool-down: 4 minutes of passive rest between sets
| Study group | Role | What they receive |
|---|---|---|
| HIIT group | Experimental | This group will only participate Wingate-based HIIT training.
In the first three weeks:
* Warm-up: Warm-up at 70 rpm for 4 minutes, including 2-3 seconds of maximal sprint at 1.30 and 2.30 minutes
* Wingate-based HIIT: HIIT 4 sets of 12x15 seconds of maximal repetitions with 15 seconds of passive rest between reps in each set
* Cool-down: 4 minutes of passive rest between sets
In the last three weeks:
* Warm-up: Warm-up at 70 rpm for 4 minutes, including 2-3 seconds of maximal sprint at 1.30 and 2.30 minutes
* Wingate-based HIIT: HIIT 5 sets of 20x15 seconds of maximal repetitions with 15 seconds of passive rest between reps in each set Cool-down: 4 minutes of passive rest between sets Receives: Other: HIIT training |
| HIIT + creatine group | Experimental | The HIIT + creatine group will participate the same Wingate-based HIIT training in addition to consume 10 g of creatine supplementation. Participants in this group will use 10 g of creatine monohydrate by dissolved in 200 ml of water. Creatine supplementation will be taken in two doses of five grams, 30 minutes before and after training. Receives: Dietary Supplement: Creatine; Other: HIIT training |
What is being measured
The primary outcome is the main question the study is designed to answer.
Wingate anaerobic power test
The Wingate anaerobic power test will be performed using a bicycle ergometer.First, a four-minute warm-up protocol with a bicycle ergometer at 60-80 rpm pedal speed and 50 W workload will be applied. Weights of 7.5% kilograms of their body weight for each participant will be placed on the pan of the bicycle ergometer and anaerobic power testing will be initiated. When they reach the pedal speed to 150 rpm, the test will start. Their maximal effort during 30-seconds will be collected. The Wingate software will be used in which the program set up with the ergometers. Information about the relevant power parameters during the test will be transmitted to the software program on the computer. All power parameters such as both absolute and relative peak power, minimum power, average power etc. will be calculated by the software program automatically.
Measured: two months
Leg strength
Back and leg dynamometer (Takei-Back \& Lift, Japan) will be used to measure leg strength. Participants will be placed their feet on the dynamometer stand. The legs will be bent at 15 angles, the body slightly forward, and the back straight. The participants will pull the dynamometer bar, which they grasped with their hands, using only their legs, without changing their current position, until the knees will be extended. Participants will repeat this trial twice, and the best value for each participant will be recorded.
Measured: two months
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted8 Aug 2023
- Study start10 Mar 2022
- Primary completion1 Aug 2022
- Study completion15 Jan 2023
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for CreatineStudies of the same condition, open and under way.
- More trials run by Ankara UniversityThe sponsor's other registered studies.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Single blind
- Who is masked
- Participant
- Enrolment
- 15 participants (actual)
Sponsor and investigators
- Lead sponsor
- Ankara University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Dicle Aras, Ankara University
- Investigators
- Dicle Aras, Study director, Ankara University Faculty of Sport Sciences
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT05981820
- Sponsor's protocol ID
- AnkaraUSport
- Status last verified
- Jul 2023
- First submitted
- 26 Apr 2023
- Last updated
- 8 Aug 2023
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Study at a glance
From the registry record, last updated by the sponsor on 8 Aug 2023. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Effect of High Intensity Interval Training and Creatine Intake on Anaerobic Power and Muscle Strength
- ClinicalTrials.gov ID
- NCT05981820
- Acronym / sponsor ID
- AnkaraUSport
- Conditions
- Creatine, High Intensity Interval Training
- Keywords
- ergogenic aids, HIIT, creatine, performance
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 15 participants (actual)
- Lead sponsor
- Ankara UniversityUniversity, hospital or other
- Study start
- 10 Mar 2022 (actual)
- Primary completion
- 1 Aug 2022 (actual)
- Study completion
- 15 Jan 2023 (actual)
- First posted
- 8 Aug 2023 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
Creatine is an ergogenic aid with positive effects on muscle and performance. The aim of the study was to determine whether there is a difference between the effects of creatine supplementation taken together with Wingate-based HIIT on anaerobic power, leg strength, heart rate variability and body composition. In the study, Wingate-based HIIT program was applied three days a week for six weeks. Anaerobic power (Wingate anaerobic power test), heart rate variability (Polar H10 chest band), leg strength (back and leg dynamometer) and body composition (PlusAvis 333) measurements of the participants were made 1 week before the training program started and the week after the training program ended. Twenty physically active adult men aged 18-30 were voluntarily included for the study. Participants were divided into two groups: Wingate-based HIIT with creatine supplementation (K-HIIT) and Wingate-based HIIT only (HIIT). As a result of the analysis, it was seen that creatine supplementation taken together with wingate-based HIIT had an effect and increased on the anaerobic power parameters, peak power, average power and minimum power values, and leg strength. In addition, it was determined that heart rate variability parameters improved heart rate (HR, beat/min) and low frequency (LF, ms2) values, but did not cause any change in body composition. Comparing and investigating the effects of different HIIT methods on anaerobic power, leg strength, heart rate variability and body composition will increase the generalizability of the study.
Detailed description
Despite the time-efficiency of HIIT in inducing chronic physiological adaptation, it depleted phosphocreatine stores in the muscle and decrease power output. It has been observed that high-intensity exercise performance increases with the increase of intramuscular phosphocreatine. It is thought that with creatine supplementation, the rate of phosphocreatine resynthesis and mitochondrial transfer of ATP to the cytoplasm may increase. This suggests that performing HIIT with creatine supplementation will provide further improvement. Therefore, the purpose of this study to compare the effectiveness of HIIT with creatine supplement compared to HIIT only. We hypothesized that creatine supplementation intake with wingate-based HIIT will positively affect power capacity and performance-related parameters.
The research will be carried out using experimental research design, one of the quantitative research methods, to measure the variables and determine the relationship between dependent and independent variables. Anaerobic power, heart rate variability, leg strength, and body composition measurements of the participants will be made 1 week before the training program started and the week after the training program ended.
The G*power software was utilized in order to determine the appropriate sample size. It has been reported that a minimum of 12 participants, 6 in each group, should be included in the study (alpha value = 0.05, 1-beta value = 0.80, and effect size = 1.6). Measurement of tests and the Wingate-based HIIT program will be conducted in the Performance Laboratory of the Faculty of Sports Sciences of Ankara University. Twenty physically active adult men aged 18-30 years, who had no history of medical and did not use drugs or ergogenic aids, will be recruited voluntarily. The ethics committee approval for the study methodology was obtained from the Ethics Committee of Ankara University Rectorate (Number: E-56786525-050.04.04/378028 Decision Number: 2022/02) and it will be carried out in compliance with the Declaration of Helsinki's principles. Written inform consents which explain the study to the participant and any right and risk will be obtained prior to data collection. Permission for the research to be carried out in the Performance Laboratory of the Faculty of Sport Sciences of Ankara University will be obtained from the Deanery of the Faculty of Sport Sciences of Ankara University. Participants will be divided into two groups as those who will perform Wingate-based HIIT with creatine (C-HIIT) (age: 21,78 ± 1,79 years; height: 179.56 ± 8.26 cm; weight: 76.86 ± 10.66 kg) and those who will perform only Wingate-based HIIT (HIIT) (age: 20.167 ± 0,98 years; height: 172.33 ± 9,88 cm ; weight: 66.53 ± 9.51kg ). Out of twenty potential participants, only 15 participants agreed to participate in the study (HIIT=6, C-HIIT=9) and 5 dropped out because of sport injuries that occured before the study process.
IBM SPSS 24.0 program (SPSS Inc., Chicago, IL, USA) will be used to statistically analyze the data obtained in the study. First of all, the distribution of the data will be evaluated to determine the test used to compare the data as parametric or non-parametric. This normality distribution assessment will be tested with Shapiro Wilk, as both groups had less than 50 participants. Determination of the mean difference in within-group statistics will be performed with Paired Sample t-Test in data with normal distribution and with Wilcoxon test in data where it is not normal. Groups will be expressed as mean ± standard error. American Psychological Association (APA) 6.0 style will be used to report statistical differences. The alpha value will be accepted as 0.05 in all statistical analysis.
This study aimed to determine whether there was a difference in the effects of taking creatine supplementation with Wingate-based HIIT on anaerobic power, leg strength, heart rate variability and body composition.
The limitation of the current research is that the study will be carried out with a single gender. In future studies on the subject, the number of participants can be increased, female participants can be included in the research and it can be examined whether there is a difference between genders and to increase the generalizability of the study, the effects of different HIIT methods on anaerobic power, leg strength, heart rate variability and body composition can be compared by investigating. In addition, the trial period may be extended as longer periods are needed to see clearer changes in HRV.
Study design
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Single blind (participant)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| HIIT group | Experimental | Other: HIIT trainingThis group will only participate Wingate-based HIIT training. In the first three weeks: * Warm-up: Warm-up at 70 rpm for 4 minutes, including 2-3 seconds of maximal sprint at 1.30 and 2.30 minutes * Wingate-based HIIT: HIIT 4 sets of 12x15 seconds of maximal repetitions with 15 seconds of passive rest between reps in each set * Cool-down: 4 minutes of passive rest between sets In the last three weeks: * Warm-up: Warm-up at 70 rpm for 4 minutes, including 2-3 seconds of maximal sprint at 1.30 and 2.30 minutes * Wingate-based HIIT: HIIT 5 sets of 20x15 seconds of maximal repetitions with 15 seconds of passive rest between reps in each set Cool-down: 4 minutes of passive rest between sets |
| HIIT + creatine group | Experimental | Dietary Supplement: Creatine, Other: HIIT trainingThe HIIT + creatine group will participate the same Wingate-based HIIT training in addition to consume 10 g of creatine supplementation. Participants in this group will use 10 g of creatine monohydrate by dissolved in 200 ml of water. Creatine supplementation will be taken in two doses of five grams, 30 minutes before and after training. |
Who may qualify
- Ages
- 18 to 30 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (3 listed)
- having no health and communication problem
- using no drugs or ergogenic aids
- being physically active adult men aged 18-30 years
Key exclusion criteria (2 listed)
- using any drugs or ergogenic aids
- having any musculoskeletal problem
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| CreatineTo observe the effects of creatine in the study, 10 g of creatine supplementation was taken only on training days during the study period. Participants used 10 g of creatine monohydrate by dissolved in 200 ml of water. Creatine supplementation was taken in two doses of five grams, 30 minutes before and after training. | Dietary supplement | HIIT + creatine group |
| HIIT trainingIn the first three weeks: Warm-up: Warm-up at 70 rpm for 4 minutes, including 2-3 seconds of maximal sprint at 1.30 and 2.30 minutes Wingate-based HIIT: HIIT 4 sets of 12x15 seconds of maximal repetitions with 15 seconds of passive rest between reps in each set Cool-down: 4 minutes of passive rest between sets In the last three weeks: Warm-up: Warm-up at 70 rpm for 4 minutes, including 2-3 seconds of maximal sprint at 1.30 and 2.30 minutes Wingate-based HIIT: HIIT 5 sets of 20x15 seconds of maximal repetitions with 15 seconds of passive rest between reps in each set Cool-down: 4 minutes of passive rest between sets | Other | HIIT + creatine group, HIIT group |
Primary outcome
Wingate anaerobic power test
Time frame: two months
The Wingate anaerobic power test will be performed using a bicycle ergometer.First, a four-minute warm-up protocol with a bicycle ergometer at 60-80 rpm pedal speed and 50 W workload will be applied. Weights of 7.5% kilograms of their body weight for each participant will be placed on the pan of the bicycle ergometer and anaerobic power testing will be initiated. When they reach the pedal speed to 150 rpm, the test will start. Their maximal effort during 30-seconds will be collected. The Wingate software will be used in which the program set up with the ergometers. Information about the relevant power parameters during the test will be transmitted to the software program on the computer. All power parameters such as both absolute and relative peak power, minimum power, average power etc. will be calculated by the software program automatically.
Leg strength
Time frame: two months
Back and leg dynamometer (Takei-Back \& Lift, Japan) will be used to measure leg strength. Participants will be placed their feet on the dynamometer stand. The legs will be bent at 15 angles, the body slightly forward, and the back straight. The participants will pull the dynamometer bar, which they grasped with their hands, using only their legs, without changing their current position, until the knees will be extended. Participants will repeat this trial twice, and the best value for each participant will be recorded.
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 8 Aug 2023.
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Dicle Aras study director
Ankara University Faculty of Sport Sciences
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Creatine and HIIT training in people with Creatine and High Intensity Interval Training, run by Ankara University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 3 inclusion and 2 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 8 Aug 2023. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Wingate anaerobic power test (time frame: two months). The study lists 2 primary outcomes in all. It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 8 Aug 2023.