Beetroot JuiceNCT06758323
Acute Effects of Beetroot Juice and Caffeine Co-ingestion on Lower-limb Strength and Muscular Endurance in Females
A study testing Beetroot juice combined with caffeine (COM) in people with Beetroot Juice and Caffeine, run by Jose Manuel Jurado Castro at 1 site in Spain.
Trial summary
The aim of this study is to evaluate the acute effects of beetroot juice and caffeine, consumed individually or in combination, on lower-limb strength and muscular endurance in physically active women. Read more about this study
- Who is it for?
- Ages 18 to 35 years, women onlyHealthy volunteers can take part
- Conditions the study is for
- Beetroot Juice, Caffeine, Placebo
- What is being tested?
- Beetroot juice combined with caffeine (COM)
- Type of treatment
- Pill or oral medicine
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 15 participants
- Where
- 1 siteSpain
- Study dates
- 1 Aug 2024to 26 Dec 2024
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 35 years
- Women only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Minimal experience of six months of strength training
- Be familiarized with the exercise test: counter movement jump (CMJ), barbell back squats, leg press and knee extension.
Who cannot join
- Supplementation consumption
- Sports injuries
Trial locations and contacts
1 site in 1 country.
About this study
Official title: Effect of Beetroot Juice Supplements with Caffeine Co-ingestion on Lower-limb Strength in Females
The aim of this study is to evaluate the acute effects of beetroot juice and caffeine, consumed individually or in combination, on lower-limb strength and muscular endurance in physically active women. The main questions it aims to answer are:
- Does the co-ingestion of beetroot juice and caffeine enhance lower-limb strength and muscular endurance more than either substance alone or a placebo?
- How do the acute effects of beetroot juice interact with caffeine on physical performance?
Read more
The study was conducted using a randomized, crossover design to evaluate the following four conditions:
Beetroot juice with placebo Caffeine with placebo Beetroot juice with caffeine Placebo only
Each participant completed all conditions, with a sufficient washout period between sessions to eliminate potential carryover effects. This design allowed for a direct comparison of the acute effects of each supplementation strategy on lower-limb strength and muscular endurance.
Participant Protocol
Participants consumed their assigned supplement on a single testing day for each condition. After supplementation, they performed standardized tests to measure lower-limb strength and muscular endurance. Progress was monitored, and data from all conditions were collected to assess the acute effects of each intervention.
- Being tested
- Beetroot juice combined with caffeine (COM), Beetroot juice placebo with caffeine (CAF), Beetroot juice with caffeine placebo (BRJ), Beetroot placebo with caffeine placebo (PLA)
- Keywords
- beetroot juice, nitrate, caffeine, resistance training, dietary supplement, female
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Beetroot juice combined with caffeine (COM)Dietary supplement
- 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session
- 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session
Beetroot juice placebo with caffeine (CAF)Dietary supplement
- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session
Beetroot juice with caffeine placebo (BRJ)Dietary supplement
- 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session
Beetroot placebo with caffeine placebo (PLA)Other
- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session
| Study group | Role | What they receive |
|---|---|---|
| COM | Experimental | Beetroot juice combined with caffeine Receives: Dietary Supplement: Beetroot juice combined with caffeine (COM) |
| CAF | Active comparator | Beetroot juice placebo with caffeine Receives: Dietary Supplement: Beetroot juice placebo with caffeine (CAF) |
| BRJ | Active comparator | Beetroot juice with caffeine placebo Receives: Dietary Supplement: Beetroot juice with caffeine placebo (BRJ) |
| PLA | Placebo comparator | Beetroot juice placebo with caffeine placebo Receives: Other: Beetroot placebo with caffeine placebo (PLA) |
What is being measured
The primary outcome is the main question the study is designed to answer.
Change of height reached in jump
Evaluated through Countermovement jump test with a recovery period of 45 seconds between jumps. The height reached was recorded in centimeters.
Measured: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Secondary outcomes (3)
Movement velocity
The velocity was recorded in meters per second (m/s) during two repetitions of the back squat, evaluated at 50% and 75% of the repetition maximum (RM).
Measured: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Muscular endurance test
The test consists of three exercises: back squat, leg press and leg extension. Three series will be performed until concentric failure at 75% RM, three minutes of rest between each series (3 series). The number of repetitions was recorded.
Measured: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Rating of perceived exertion (RPE).
Immediately at the end of the muscular endurance test, participants will be instructed to report their RPE using a scale 1-10 (worse to better outcome).
Measured: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted3 Jan 2025
- Study start1 Aug 2024
- Primary completion26 Dec 2024
- Study completion26 Dec 2024
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Beetroot JuiceStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Model description
- Double-Blind Crossover Randomized Trial
- Masking
- Double blind
- Who is masked
- Participant, Investigator
- Enrolment
- 15 participants (actual)
Sponsor and investigators
- Lead sponsor
- Jose Manuel Jurado Castro
- Sponsor type
- University, hospital or other
- Responsible party
- Sponsor-investigator, Jose Manuel Jurado Castro, Maimónides Biomedical Research Institute of Córdoba
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT06758323
- Sponsor's protocol ID
- IMIBIC_BRJ_CAF
- Status last verified
- Jan 2025
- First submitted
- 26 Dec 2024
- Last updated
- 6 Jan 2025
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Study at a glance
From the registry record, last updated by the sponsor on 6 Jan 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Effect of Beetroot Juice Supplements with Caffeine Co-ingestion on Lower-limb Strength in Females
- ClinicalTrials.gov ID
- NCT06758323
- Acronym / sponsor ID
- IMIBIC_BRJ_CAF
- Keywords
- beetroot juice, nitrate, caffeine, resistance training, dietary supplement, female
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 15 participants (actual)
- Lead sponsor
- Jose Manuel Jurado CastroUniversity, hospital or other
- Study start
- 1 Aug 2024 (actual)
- Primary completion
- 26 Dec 2024 (actual)
- Study completion
- 26 Dec 2024 (actual)
- First posted
- 3 Jan 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The aim of this study is to evaluate the acute effects of beetroot juice and caffeine, consumed individually or in combination, on lower-limb strength and muscular endurance in physically active women. The main questions it aims to answer are:
- Does the co-ingestion of beetroot juice and caffeine enhance lower-limb strength and muscular endurance more than either substance alone or a placebo?
- How do the acute effects of beetroot juice interact with caffeine on physical performance?
The study was conducted using a randomized, crossover design to evaluate the following four conditions:
Beetroot juice with placebo Caffeine with placebo Beetroot juice with caffeine Placebo only
Each participant completed all conditions, with a sufficient washout period between sessions to eliminate potential carryover effects. This design allowed for a direct comparison of the acute effects of each supplementation strategy on lower-limb strength and muscular endurance.
Participant Protocol
Participants consumed their assigned supplement on a single testing day for each condition. After supplementation, they performed standardized tests to measure lower-limb strength and muscular endurance. Progress was monitored, and data from all conditions were collected to assess the acute effects of each intervention.
Study design
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Double blind (participant, investigator)
- Number of arms
- 4
- Model description
- Double-Blind Crossover Randomized Trial
| Arm | Type | Receives |
|---|---|---|
| COM | Experimental | Dietary Supplement: Beetroot juice combined with caffeine (COM)Beetroot juice combined with caffeine |
| CAF | Active comparator | Dietary Supplement: Beetroot juice placebo with caffeine (CAF)Beetroot juice placebo with caffeine |
| BRJ | Active comparator | Dietary Supplement: Beetroot juice with caffeine placebo (BRJ)Beetroot juice with caffeine placebo |
| PLA | Placebo comparator | Other: Beetroot placebo with caffeine placebo (PLA)Beetroot juice placebo with caffeine placebo |
Who may qualify
- Ages
- 18 to 35 years
- Sex
- Female only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (2 listed)
- Minimal experience of six months of strength training
- Be familiarized with the exercise test: counter movement jump (CMJ), barbell back squats, leg press and knee extension.
Key exclusion criteria (2 listed)
- Supplementation consumption
- Sports injuries
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Beetroot juice combined with caffeine (COM)* 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session * 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session | Dietary supplement | COM |
| Beetroot juice placebo with caffeine (CAF)- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - 200 mg caffeine capsule from the IO.GENIX Nutrition brand (Toledo, Spain) 60 minutes before experimental session | Dietary supplement | CAF |
| Beetroot juice with caffeine placebo (BRJ)- 70 mL of beetroot juice (400 mg NO3-, BEET It Sport; James White Drinks Ltd) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session | Dietary supplement | BRJ |
| Beetroot placebo with caffeine placebo (PLA)- 70 mL of a blackcurrant beverage, which depletes NO3- (Capri-Sun) 120 minutes before experimental session - Multivitamin capsule from NaturrTierra (Leganés, Spain) 60 minutes before experimental session | Other | PLA |
Primary outcome
Change of height reached in jump
Time frame: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Evaluated through Countermovement jump test with a recovery period of 45 seconds between jumps. The height reached was recorded in centimeters.
3 secondary outcomes
Movement velocity
Time frame: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Muscular endurance test
Time frame: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Rating of perceived exertion (RPE).
Time frame: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 6 Jan 2025.
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Beetroot juice combined with caffeine (COM) in people with Beetroot Juice and Caffeine, run by Jose Manuel Jurado Castro at 1 site in Spain.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 2 inclusion and 2 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 6 Jan 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Spain. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Change of height reached in jump (time frame: Visit 1 (Day 1), Visit 2 (Day 5), Visit 3 (Day 9), Visit 4 (Day 13)). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 6 Jan 2025.