KetosisNCT06769100
Exogenous Ketosis and Muscle Protein Synthesis During Exercise Recovery
A study testing Ketone Monoester (KET), Ketone Monoester + Whey Protein (KET+PRO) and Whey Protein (PRO) in people with Ketosis and Muscle Protein Synthesis, run by McGill University at 1 site in Canada.
Trial summary
An acute bout of resistance exercise stimulates muscle protein synthesis (MPS) rates for up to 24-48 hours, supporting muscle growth and repair. To optimize the anabolic effects of resistance exercise, the provision of dietary amino acids (i.e., proteins) is essential. Read more about this study
- Who is it for?
- Ages 18 to 40 yearsHealthy volunteers can take part
- Conditions the study is for
- Ketosis, Muscle Protein Synthesis, Dietary Proteins
- What is being tested?
- Ketone Monoester (KET), Ketone Monoester + Whey Protein (KET+PRO) and Whey Protein (PRO)
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 48 participants
- Where
- 1 siteCanada
- Study dates
- 10 Jan 2025to 2 Mar 2026
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 40 years
- All sexes
- Healthy volunteers accepted
- Adult
Who may be able to join
- Healthy adult female or male participants who are 18-40 years of age (inclusive) BMI >18.5 and <30.0 kg/m2
- Moderately active (i.e., ≥ 1 session of lower-body weightlifting /week for the previous 2 months).
- Has maintained stable use of medication and supplements (which are not limited by the exclusion criteria), stable dietary and lifestyle habits, and stable body weight, for the last 3 months prior to screening and agree to maintain them throughout the study.
- Be willing to entirely avoid alcohol consumption 48hr prior to the experimental test day.
- Willing and able to agree to the requirements and restrictions of this study, be willing to give voluntary consent, be able to understand and read the questionnaires, and carry out all study-related procedures.
Who cannot join
- Females who are lactating or pregnant
- Females using third-generation oral contraceptives (including: Desogen®, Ortho-Cept, Ortho-Cyclen, Ortho Tri-Cyclen) as these are known to affect protein metabolism in females.
- Individuals with metabolic disorders including: Type I or Type II diabetes
- Individuals with a history of thrombosis / cardiovascular disease
- Individuals who use of anticoagulants
- Individuals with musculoskeletal / orthopedic disorders
Show all 17Show fewer
- Individuals with knee injuries (i.e., ACL injuries).
- Individuals who have used tobacco products within the last 6 months
- Individuals with a history of neuromuscular problems
- Chronic usage of medications known to modulate skeletal muscle metabolism (i.e. corticosteroids, hormone replacement therapy (HRT), and over-the-counter supplements including creatine monohydrate) in the last 6 months.
- Individuals with allergies to milk proteins (whey or casein).
- Individuals with lactose intolerance
- Individuals with Phenylketonuria (PKU)
- Previous participation in amino acid tracer studies.
- Adherence to a vegetarian or vegan diet
- Current use of ketone supplements or adherence to a ketogenic diet
- Individuals who train more than ≥ 5 sessions of lower-body weightlifting /week for the previous 4 months
Trial locations and contacts
1 site in 1 country.
About this study
Official title: The Effects of Ketone Monoester Intake on Postprandial Myofibrillar Protein Synthesis Rates in Young Adults During Recovery Following an Acute Bout of Resistance Exercise
An acute bout of resistance exercise stimulates muscle protein synthesis (MPS) rates for up to 24-48 hours, supporting muscle growth and repair. To optimize the anabolic effects of resistance exercise, the provision of dietary amino acids (i.e., proteins) is essential. Dietary protein intake provides the body with necessary amounts of essential and non-essential amino acids, which represent the building blocks for muscle proteins, enhancing anabolic muscle growth. The ingestion of dietary protein, such as whey protein, is well established to stimulate an increase in the rate of protein synthesis in skeletal muscle following resistance exercise. Research has demonstrated a dose-dependent relationship between protein intake and MPS rate, with 25 grams being the optimal dose to maximally stimulate MPS rates in younger adults with excess protein oxidized as a fuel source.
Determining whether this maximally stimulated MPS response can be further heightened during post-exercise recovery using non-protein dietary factors is yet to be explored. Recently, it has been shown that novel orally ingested ketone body supplements can stimulate MPS rates in younger adults at rest.
Read more
Ketone bodies (β-OHB) are lipid- derived molecules normally produced under conditions of glucose deprivation (i.e., fasting/starvation, or a low carbohydrate 'ketogenic' diet). However, these orally ingested ketone supplements rapidly increase blood ketone levels without the need for dietary restriction6. In vitro research showed that the combination of leucine and ketone bodies stimulated a 2-fold increase in MPS, compared to the leucine group alone, indicating synergistic effects of protein and ketone bodies on MPS. However, the effect of ketone supplementation, with and without dietary protein co-ingestion, on MPS rate during post-exercise recovery is yet to be investigated. If ketone bodies can amplify the anabolic response to dietary protein, they may provide a novel approach to maximizing muscle adaptation during post-exercise recovery.
Therefore, the purpose of this study is to evaluate the effects of ketone monoester intake on postprandial muscle protein synthesis rates when consumed alone and when co-ingested with an optimal dose (25 g) of whey protein during recovery after resistance exercise compared to 1) an optimal dose of whey protein (25 g), and 2) a control flavored water. It is hypothesized that muscle protein synthesis rates will be stimulated following the ingestion of the ketone body beverage. Further, muscle protein synthesis rates will be further enhanced when the ketone-containing beverage and an optimal dose are taken together.
Read the detailed description
A parallel group design will be used for this randomized double-blind, placebo-controlled study in healthy adults to investigate the effects of ketone monoester on myofibrillar protein synthesis rates during post-exercise recovery. There are 4 groups in this trial, including one ketone group (0.36g/kg body weight) (KET), one protein group (25 g whey protein) (PRO), a combination of ketone and protein (KET+PRO), and a placebo group (flavoured water) (CON). A total of 48 participants will be enrolled in this trial (n=12 per group).
The study will include a screening visit (visit 1), 10-repetition maximum (10-RM) testing (visit 2), where participants' 10-RM will be determined for the exercise protocol, and the experimental trial (visit 3).
During the experimental trials, participants will arrive to the laboratory in a fasted state, and a prime dose of the L-[ring-2H5]-phenylalanine will be administered followed by a continuous infusion at a rate 0.05 μmol/kg of body weight/min. Then, participants will perform a unilateral lower-body resistance exercise, consisting of 8 sets of 10 reps of unilateral leg extension at 90% of their 10-RM with 90 seconds rest in between sets. Following exercise, the nutritional treatment will be administered.
Arterialized blood will be collected at baseline and 13 postprandial timepoints across 9 hours for plasma amino acid, glucose, and insulin quantitation.
Additionally, changes in capillary blood β-HB concentration will be assessed throughout the trial by collecting capillary blood samples at baseline and 10 postprandial timepoints. Finally, muscle biopsy samples from both the exercised leg and the rested leg will be collected prior to beverage intake and at the 5-hour mark of the postprandial post-recovery period to assess myofibrillar protein synthesis rates.
- Being tested
- Ketone Monoester (KET), Ketone Monoester + Whey Protein (KET+PRO), Whey Protein (PRO), Flavour matched placebo (CON), Resistance exercise
- Keywords
- Ketosis, Regulation of Muscle Protein Synthesis, Resistance Exercise, Post-Exercise Recovery, Whey Protein
- Medical subject headings
- Ketosis
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Ketone Monoester (KET)Dietary supplement
- Ketone monoester supplement (R)-3-hydroxybutyl (R)-3-hydroxybutyrate based on participants' body weight (0.36g/kg body weight). The ketone brand name: delta G Oxford Ketone Ester
Ketone Monoester + Whey Protein (KET+PRO)Dietary supplement
- Ketone monoester supplement (R)-3-hydroxybutyl (R)-3-hydroxybutyrate based on participants' body weight (0.36g/kg body weight)
- 25g Whey Protein
- L-[ring-2H5]-phenylalanine tracer (enriched to 4%)
Whey Protein (PRO)Dietary supplement
- 25g Whey Protein
- L-[ring-2H5]-phenylalanine tracer (enriched to 4%)
Flavour matched placebo (CON)Dietary supplement
- Flavoured water (non-caloric bitter + citrus flavours)
Resistance exerciseOther
- 8 sets of 10 reps at 90% of 10- repetition maximum (10-RM) of unilateral leg extension with 90 seconds rest in between sets.
| Study group | Role | What they receive |
|---|---|---|
| Ketone Monoester (KET) | Experimental | Ketone monoester supplement (R)-3-hydroxybutyl (R)-3-hydroxybutyrate based on participants' body weight (0.36g/kg body weight). Receives: Dietary Supplement: Ketone Monoester (KET); Other: Resistance exercise |
| Ketone Monoester + Whey Protein (KET+PRO) | Experimental | Ketone monoester supplement (R)-3-hydroxybutyl (R)-3-hydroxybutyrate based on participants' body weight (0.36g/kg body weight) and 25g of whey protein. Receives: Dietary Supplement: Ketone Monoester + Whey Protein (KET+PRO); Other: Resistance exercise |
| Whey Protein (PRO) | Experimental | 25g of whey protein. Receives: Dietary Supplement: Whey Protein (PRO); Other: Resistance exercise |
| Placebo drink (CON) | Placebo comparator | Flavoured water. Receives: Dietary Supplement: Flavour matched placebo (CON); Other: Resistance exercise |
What is being measured
The primary outcome is the main question the study is designed to answer.
Myofibrillar fractional synthesis rate
Quantification of changes in basal myofibrillar fractional synthetic rate (%/hour) in the rested and exercised limbs.
Measured: 0 - 5 hours in the postprandial period.
Secondary outcomes (14)
Time-course data for plasma enrichments (in moles percent excess) of L-[ring-2H5]-phenylalanine
Changes in plasma-free L-[ring-2H5]-phenylalanine enrichment measured at 14 time points (t = -180, -120, -60, 0, 15, 30, 45, 60, 90, 120, 150, 180, 240, 300 minute).
Measured: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for total amino acid concentration
Incremental area under the curve (iAUC) for plasma concentration of total amino acids (μmol/L) including: free leucine, isoleucine, valine, histidine, lysine, methionine, phenylalanine, threonine, tryptophan, arginine, glutamine, glycine, alanine, serine, glutamic acid, aspartic acid, asparagine, tyrosine, cysteine, proline (combined), measured at baseline and across the 3-hour pre-prandial period and 5-hour postprandial period.
Measured: 3 hours pre-prandial to 5 hours postprandial
Time-course data for total amino acid concentration
Changes in plasma concentration of total amino acid concentration (μmol/L) including: free leucine, isoleucine, valine, histidine, lysine, methionine, phenylalanine, threonine, tryptophan, arginine, glutamine, glycine, alanine, serine, glutamic acid, aspartic acid, asparagine, tyrosine, cysteine, proline (combined), measured at 14 time points (t = -180, -120, -60, 0, 15, 30, 45, 60, 90, 120, 150, 180, 240, 300 minute).
Measured: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for essential amino acid concentration
Incremental area under the curve (iAUC) for plasma essential amino acid concentration (μmol/L) including free leucine, isoleucine, valine, histidine, lysine, methionine, phenylalanine, threonine, tryptophan (combined), measured at baseline and across the 3-hour pre-prandial period and 5-hour postprandial period.
Measured: 3 hours pre-prandial to 5 hours postprandial
Time-course data for essential amino acid concentration
Changes in plasma concentration of essential amino acid (μmol/L) including: free leucine, isoleucine, valine, histidine, lysine, methionine, phenylalanine, threonine, tryptophan (combined), measured at 14 time points (t = -180, -120, -60, 0, 15, 30, 45, 60, 90, 120, 150, 180, 240, 300 minute).
Measured: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for leucine concentration
Incremental area under the curve (iAUC) for plasma leucine concentration (μmol/L) measured at baseline and across the 3-hour pre-prandial period and 5-hour postprandial period.
Measured: 3 hours pre-prandial to 5 hours postprandial
Time-course data for leucine concentration
Changes in plasma concentration of leucine (μmol/L) measured at 14 time points (t = -180, -120, -60, 0, 15, 30, 45, 60, 90, 120, 150, 180, 240, 300 minute).
Measured: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for glucose concentration
Plasma glucose concentration (mmol/L) and its corresponding incremental area under the curve (iAUC), measured at baseline and across the 3-hour pre-prandial period and 5-hour postprandial period.
Measured: 3 hours pre-prandial to 5 hours post-prandial
Time-course data for glucose concentration
Changes in plasma concentration of glucose (mmol/L) measured at 14 time points (t = -180, -120, -60, 0, 15, 30, 45, 60, 90, 120, 150, 180, 240, 300 minute).
Measured: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for insulin concentration
Incremental area under the curve (iAUC) for plasma concentration of insulin (pmol/L), measured at baseline and across the 3-hour pre-prandial period and 5-hour postprandial period.
Measured: 3 hours pre-prandial to 5 hours post-prandial
Time-course data for insulin concentration
Changes in plasma concentration of insulin (pmol/L) measured at 14 time points (t = -180, -120, -60, 0, 15, 30, 45, 60, 90, 120, 150, 180, 240, 300 minute).
Measured: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for β-HB concentrations
Capillary blood β-OHB concentration (mmol/L) and its corresponding incremental area under the curve (iAUC) measured during the pre-prandial period and postprandial period.
Measured: 1 hour pre-prandial to 5 hours postprandial
Time-course data for β-HB concentrations
Changes in capillary blood β-OHB concentration (mmol/L) measured at 10 time points (t = -60, 0, 30, 60, 90, 120, 150, 180, 240, 300 minute).
Measured: 1 hour pre-prandial to 5 hours in the post-prandial period
Changes in the phosphorylation status of anabolic signaling molecules
Western blot analysis will be used to measure the changes in the basal phosphorylation status of anabolic signaling molecules, including p-mTORC1 (Ser2448), p-p70S6K (Thr389), p-Akt (Ser473), p-4E-BP1 (Thr37/46), and p-rpS6 (Ser240/244), p-ERK1(Thr202/Tyr204), and p-ERK2 (Thr185/Tyr187), in the exercised and the rested limbs.
Measured: 0 and 5 hours of the postprandial period.
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted10 Jan 2025
- Study start10 Jan 2025
- Primary completion2 Mar 2026
- Study completion2 Mar 2026
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for KetosisStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Triple blind
- Who is masked
- Participant, Investigator, Outcomes assessor
- Enrolment
- 48 participants (actual)
Sponsor and investigators
- Lead sponsor
- McGill University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Tyler Churchward-Venne, McGill University
- Investigators
- Tyler Churchward-Venne, PhD, Principal investigator, Department of Kinesiology and Physical Education, McGill University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT06769100
- Sponsor's protocol ID
- A00-M15-24A
- Status last verified
- Mar 2026
- First submitted
- 6 Jan 2025
- Last updated
- 23 Mar 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
Study at a glance
From the registry record, last updated by the sponsor on 23 Mar 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Effects of Ketone Monoester Intake on Postprandial Myofibrillar Protein Synthesis Rates in Young Adults During Recovery Following an Acute Bout of Resistance Exercise
- ClinicalTrials.gov ID
- NCT06769100
- Acronym / sponsor ID
- A00-M15-24A
- Keywords
- Ketosis, Regulation of Muscle Protein Synthesis, Resistance Exercise, Post-Exercise Recovery, Whey Protein
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 48 participants (actual)
- Lead sponsor
- McGill UniversityUniversity, hospital or other
- Study start
- 10 Jan 2025 (actual)
- Primary completion
- 2 Mar 2026 (actual)
- Study completion
- 2 Mar 2026 (actual)
- First posted
- 10 Jan 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
An acute bout of resistance exercise stimulates muscle protein synthesis (MPS) rates for up to 24-48 hours, supporting muscle growth and repair. To optimize the anabolic effects of resistance exercise, the provision of dietary amino acids (i.e., proteins) is essential. Dietary protein intake provides the body with necessary amounts of essential and non-essential amino acids, which represent the building blocks for muscle proteins, enhancing anabolic muscle growth. The ingestion of dietary protein, such as whey protein, is well established to stimulate an increase in the rate of protein synthesis in skeletal muscle following resistance exercise. Research has demonstrated a dose-dependent relationship between protein intake and MPS rate, with 25 grams being the optimal dose to maximally stimulate MPS rates in younger adults with excess protein oxidized as a fuel source.
Determining whether this maximally stimulated MPS response can be further heightened during post-exercise recovery using non-protein dietary factors is yet to be explored. Recently, it has been shown that novel orally ingested ketone body supplements can stimulate MPS rates in younger adults at rest.
Ketone bodies (β-OHB) are lipid- derived molecules normally produced under conditions of glucose deprivation (i.e., fasting/starvation, or a low carbohydrate 'ketogenic' diet). However, these orally ingested ketone supplements rapidly increase blood ketone levels without the need for dietary restriction6. In vitro research showed that the combination of leucine and ketone bodies stimulated a 2-fold increase in MPS, compared to the leucine group alone, indicating synergistic effects of protein and ketone bodies on MPS. However, the effect of ketone supplementation, with and without dietary protein co-ingestion, on MPS rate during post-exercise recovery is yet to be investigated. If ketone bodies can amplify the anabolic response to dietary protein, they may provide a novel approach to maximizing muscle adaptation during post-exercise recovery.
Therefore, the purpose of this study is to evaluate the effects of ketone monoester intake on postprandial muscle protein synthesis rates when consumed alone and when co-ingested with an optimal dose (25 g) of whey protein during recovery after resistance exercise compared to 1) an optimal dose of whey protein (25 g), and 2) a control flavored water. It is hypothesized that muscle protein synthesis rates will be stimulated following the ingestion of the ketone body beverage. Further, muscle protein synthesis rates will be further enhanced when the ketone-containing beverage and an optimal dose are taken together.
Detailed description
A parallel group design will be used for this randomized double-blind, placebo-controlled study in healthy adults to investigate the effects of ketone monoester on myofibrillar protein synthesis rates during post-exercise recovery. There are 4 groups in this trial, including one ketone group (0.36g/kg body weight) (KET), one protein group (25 g whey protein) (PRO), a combination of ketone and protein (KET+PRO), and a placebo group (flavoured water) (CON). A total of 48 participants will be enrolled in this trial (n=12 per group).
The study will include a screening visit (visit 1), 10-repetition maximum (10-RM) testing (visit 2), where participants' 10-RM will be determined for the exercise protocol, and the experimental trial (visit 3).
During the experimental trials, participants will arrive to the laboratory in a fasted state, and a prime dose of the L-[ring-2H5]-phenylalanine will be administered followed by a continuous infusion at a rate 0.05 μmol/kg of body weight/min. Then, participants will perform a unilateral lower-body resistance exercise, consisting of 8 sets of 10 reps of unilateral leg extension at 90% of their 10-RM with 90 seconds rest in between sets. Following exercise, the nutritional treatment will be administered.
Arterialized blood will be collected at baseline and 13 postprandial timepoints across 9 hours for plasma amino acid, glucose, and insulin quantitation.
Additionally, changes in capillary blood β-HB concentration will be assessed throughout the trial by collecting capillary blood samples at baseline and 10 postprandial timepoints. Finally, muscle biopsy samples from both the exercised leg and the rested leg will be collected prior to beverage intake and at the 5-hour mark of the postprandial post-recovery period to assess myofibrillar protein synthesis rates.
Study design
- Primary purpose
- Basic science
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Triple blind (participant, investigator, outcomes assessor)
- Number of arms
- 4
| Arm | Type | Receives |
|---|---|---|
| Ketone Monoester (KET) | Experimental | Dietary Supplement: Ketone Monoester (KET), Other: Resistance exerciseKetone monoester supplement (R)-3-hydroxybutyl (R)-3-hydroxybutyrate based on participants' body weight (0.36g/kg body weight). |
| Ketone Monoester + Whey Protein (KET+PRO) | Experimental | Dietary Supplement: Ketone Monoester + Whey Protein (KET+PRO), Other: Resistance exerciseKetone monoester supplement (R)-3-hydroxybutyl (R)-3-hydroxybutyrate based on participants' body weight (0.36g/kg body weight) and 25g of whey protein. |
| Whey Protein (PRO) | Experimental | Dietary Supplement: Whey Protein (PRO), Other: Resistance exercise25g of whey protein. |
| Placebo drink (CON) | Placebo comparator | Dietary Supplement: Flavour matched placebo (CON), Other: Resistance exerciseFlavoured water. |
Who may qualify
- Ages
- 18 to 40 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (5 listed)
- Healthy adult female or male participants who are 18-40 years of age (inclusive) BMI >18.5 and <30.0 kg/m2
- Moderately active (i.e., ≥ 1 session of lower-body weightlifting /week for the previous 2 months).
- Has maintained stable use of medication and supplements (which are not limited by the exclusion criteria), stable dietary and lifestyle habits, and stable body weight, for the last 3 months prior to screening and agree to maintain them throughout the study.
- Be willing to entirely avoid alcohol consumption 48hr prior to the experimental test day.
- Willing and able to agree to the requirements and restrictions of this study, be willing to give voluntary consent, be able to understand and read the questionnaires, and carry out all study-related procedures.
Key exclusion criteria (17 listed)
- Females who are lactating or pregnant
- Females using third-generation oral contraceptives (including: Desogen®, Ortho-Cept, Ortho-Cyclen, Ortho Tri-Cyclen) as these are known to affect protein metabolism in females.
- Individuals with metabolic disorders including: Type I or Type II diabetes
- Individuals with a history of thrombosis / cardiovascular disease
- Individuals who use of anticoagulants
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Ketone Monoester (KET)- Ketone monoester supplement (R)-3-hydroxybutyl (R)-3-hydroxybutyrate based on participants' body weight (0.36g/kg body weight). The ketone brand name: delta G Oxford Ketone Ester | Dietary supplement | Ketone Monoester (KET) |
| Ketone Monoester + Whey Protein (KET+PRO)* Ketone monoester supplement (R)-3-hydroxybutyl (R)-3-hydroxybutyrate based on participants' body weight (0.36g/kg body weight) * 25g Whey Protein * L-[ring-2H5]-phenylalanine tracer (enriched to 4%) | Dietary supplement | Ketone Monoester + Whey Protein (KET+PRO) |
| Whey Protein (PRO)* 25g Whey Protein * L-[ring-2H5]-phenylalanine tracer (enriched to 4%) | Dietary supplement | Whey Protein (PRO) |
| Flavour matched placebo (CON)- Flavoured water (non-caloric bitter + citrus flavours) | Dietary supplement | Placebo drink (CON) |
| Resistance exercise- 8 sets of 10 reps at 90% of 10- repetition maximum (10-RM) of unilateral leg extension with 90 seconds rest in between sets. | Other | Ketone Monoester (KET), Ketone Monoester + Whey Protein (KET+PRO), Placebo drink (CON), Whey Protein (PRO) |
Primary outcome
Myofibrillar fractional synthesis rate
Time frame: 0 - 5 hours in the postprandial period.
Quantification of changes in basal myofibrillar fractional synthetic rate (%/hour) in the rested and exercised limbs.
14 secondary outcomes
Time-course data for plasma enrichments (in moles percent excess) of L-[ring-2H5]-phenylalanine
Time frame: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for total amino acid concentration
Time frame: 3 hours pre-prandial to 5 hours postprandial
Time-course data for total amino acid concentration
Time frame: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for essential amino acid concentration
Time frame: 3 hours pre-prandial to 5 hours postprandial
Time-course data for essential amino acid concentration
Time frame: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for leucine concentration
Time frame: 3 hours pre-prandial to 5 hours postprandial
Time-course data for leucine concentration
Time frame: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for glucose concentration
Time frame: 3 hours pre-prandial to 5 hours post-prandial
Time-course data for glucose concentration
Time frame: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for insulin concentration
Time frame: 3 hours pre-prandial to 5 hours post-prandial
Time-course data for insulin concentration
Time frame: Baseline, 3 hours pre-prandial, and 5 hours into the postprandial period.
Incremental area under the curve for β-HB concentrations
Time frame: 1 hour pre-prandial to 5 hours postprandial
Time-course data for β-HB concentrations
Time frame: 1 hour pre-prandial to 5 hours in the post-prandial period
Changes in the phosphorylation status of anabolic signaling molecules
Time frame: 0 and 5 hours of the postprandial period.
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 23 Mar 2026.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Tyler Churchward-Venne, PhD principal investigator
Department of Kinesiology and Physical Education, McGill University
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Ketone Monoester (KET), Ketone Monoester + Whey Protein (KET+PRO) and Whey Protein (PRO) in people with Ketosis and Muscle Protein Synthesis, run by McGill University at 1 site in Canada.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 5 inclusion and 17 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 23 Mar 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Canada. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Myofibrillar fractional synthesis rate (time frame: 0 - 5 hours in the postprandial period.). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 23 Mar 2026.