Impact of Massage on Daytime Napping

The current study will assess the impact of two different massage styles in aspects related to relaxation and wakefulness using a polysomnography system and questionnaire measurements.

The volunteers of the current study will participate in 3 different scenarios, each 5 days apart: Scenario 1 is the control session when participants will lay down on the prone position on a professional massage bed for 35 min without any interventions, Scenario 2 will be the experimental session 1 where participants will lay down on the prone position on a professional massage bed for 35 min receiving a deep relaxation massage session, and Scenario 3 will be the experimental session 2, where participants will lay down on the prone position on a professional massage bed for 35 min receiving a Activation/Stimulation type of massage session. During all three scenarios participants' brain signal and subjective perceived feeling of relaxation will be recorded. Scenario 2 and 3 are taking place in a random order in every participant.

Materials and Methods Subjects Fifteen (N=15) participants will be included in the current study. Inclusion criteria include subjects of both sexes, aged >18 years, and Pittsburgh Quality Sleep Index score >5. Exclusion criteria include a history of mental illness, dermatological diseases, allergies to massage oil, history of epilepsy and any acute or chronic condition that would limit the ability of the patient to participate in the study.

Study Design Subjects will randomly be assigned into 3 conditions each one week apart: 1) the control condition (CON) where subjects will not receive any intervention, while they are rested for 35 min on a massage bed; 2) the activation condition (ACT) where subjects will receive an activation (Sports) massage session lasting for 30 min and rest for 5 min without any interference; and 3) the relaxation condition (REL) where subjects receive a relaxation massage session lasting for 30 min and rest for 5 min without any interference (Figure 2). All conditions will be separated one week apart and take place at the exact time of the day, while the order of the various conditions is randomly assigned. All massage sessions were delivered by a professional masseur, while the researchers analyzing the data will be blind to the assigned group. All measurements will be conducted at the sleep laboratory under controlled conditions, with a constant room temperature of 22°C. The study is approved by the Human Research and Ethics Committee of the University of Thessaly (2094-2/08-02-2023). All Subjects will give their written informed consent prior to study participation.

Procedure All measurements will be performed in the Lifestyle Medicine Laboratory, at the Department of Physical Education and Sports Science of Trikala, in Thessaly, Greece.

Before the initiation of the study, subjects will complete a series of questionnaires and measurements related to overall health. Subjects will be connected to a portable polysomnographic EEG/EOG system (HST-mit-tablet, SOMNOmedics AG, Randersacker, Germany) for the recording of brain activity. Before and after each condition, subjects will complete a relaxation sensation questionnaire (RSQ) for assessing the state of relaxation and perform 3 maximum handgrip attempts to assess the level of muscle strength. Measurements of vital signs such as resting heart rate and blood pressure also will be recorded. The participants will be free to either sleep or stay awake during the whole intervention period.

Measuring Instruments Body Composition Body composition will be assessed using anthropometric measurements including BMI, and bioimpedance (Tanita DC-360 S, Serinth) under standard methodology.

Questionnaires The following questionnaires will be administered using the interview method by experienced researchers.

• The Pittsburgh Sleep Quality Index (PSQI) will be used to assess sleep quality and the existence of any sleep abnormalities.
• The Short Form survey 36-item 36 quality of life questionnaire (SF-36) will be used to assess the quality of life.
• The Beck Depression Inventory (BDI) questionnaire will be used to assess depressive symptoms and signs.
• The Perceived Stress Scale (PSS) will be used to assess the level of stress.
• The Relaxation State Questionnaire (RSQ) will be used to assess the state of relaxation.

Brain Activity / Arousal Assessment The Home Sleep Test (HST) (www.somnomedics.de), will be used to assess brain activity during napping periods. This is a portable sleep monitoring system that enables the recording of various sleep parameters in a natural setting, as the patient's bedroom. EEG signals will be analyzed in 30 sec epochs using the SOMNOmedics PSG analysis software (Domino panel ver. 3.0.0.8) and manual editing. The setup for the polysomnography (PSG) involved the use of the Home-Sleep-Test REM+ device, which records various sleep parameters, including EEG, EOG, and EMG. EEG measurements will be obtained using silver/silver chloride electrode sets, placed in four specific locations that allow for the monitoring of key brain regions associated with sleep cycles. These locations will be selected based on the standardized 10/20 system to ensure accurate representation of the frontal, central, and occipital regions, facilitating a comprehensive analysis of the sleep architecture. The use of these electrodes provides high-fidelity recordings essential for evaluating both the depth and quality of sleep stages.

For EEG, the electrode will be positioned in the frontopolar region, specifically at Fp1 (left side) and M1, allowing for the monitoring of brain activity. The EOG electrodes will be placed near the left and right eyes to record eye movements, which is crucial for analyzing sleep stages, including REM sleep. The system is designed for ease of use: a tablet guides the patient through the sensor application process. Once the test begins, the tablet records data that the sensors transfer via Bluetooth. After the sleep study is completed, all data is automatically transferred from the tablet to a cloud platform for comprehensive analysis, ensuring seamless and accurate evaluation of sleep parameters such as brain activity, eye movements, and muscle tone. EEG analysis will be reported as follows: Total Sleep Time (total amount of sleep time scored during the total recording time); Sleep Efficiency (Total sleep time/Time in bed); Sustained Sleep Efficiency (Total sleep time/Time in bed - Sleep latency stage 2); Sleep Latency (the period of time it takes for a person to fall asleep after they have gone to bed and tried to initiate sleep); Sleep Latency N1 (the period of time between wakefulness and when sleep begins); Sleep Latency N2 (the period of time between time in bed and sleep onset stage 2); REM Latency (the amount of time elapsed between the onset of sleep to the first REM stage).

Handgrip strength assessment The handgrip test will be used for the assessment of maximum isometric strength of the hand and arm muscles (Marsden MG-4800 Hand Dynamometer) and used as a measurement of muscle tone alertness. Reduction of muscle strength after the intervention, was assessed as reduction of muscle tone.

Massage Sessions

Activation (Sports) massage The activation (sports) type of massage will be used as one of the massage interventions. This technique will be performed on a full body with more attention given to specific leg muscle groups such as the feet, the gastrocnemius, the femoral biceps and quadriceps and in upper body muscle groups such as the triceps, the rhomboid, the deltoid, the trapezius and head areas. Briefly, this type of massage involves a wide range of techniques that include effleurage, palmar sliding, pounding and hacking. The total duration of the activation message will be 30 minutes.

Relaxation massage The Relaxation type of massage will be used as the second type of intervention in this study. This technique is designed to promote deep relaxation, reduce stress and enhance overall well-being. It is characterized by the use of four primary techniques performed on the full body, including the palms and feet. Specifically, these techniques consist of effleurage (long, smooth, gliding strokes), petrissage (light pressure, rhythmic movements) and vibration gentle shaking movements. In addition to these, the technique also incorporates rhythmic movements and deep light pressure to enhance relaxation and alleviate tension. The total duration of the Relaxation massage will be 30 minutes.

Statistical Analysis The statistical analysis will be performed using IBM SPSS Statistics version 29 (SPSS Inc., Chicago, U.S.A.) An independent samples T-test will be used to examine differences in baseline characteristics and questionnaires between male and female subjects. A General Linear Model (GLM) Repeated Measures ANOVA will be used to assess changes in all parameters among the 3 different scenarios. A Bonferroni post-hoc test will be performed to assess individual differences. To assess normality, the Shapiro-Wilk test will be used alongside graphical representations, including the Normal Q-Q plot, Detrended Normal Q-Q plot, and Box Plot. The significance level is set at 5%. Beyond significance testing (p-value), effect size will also be considered to evaluate the magnitude of the effect.

Power Analysis Sample size calculations has been conducted using G*Power 3.1. The post-hoc "GLM" - Repeated measures, within factors" method was used to calculate the power analysis. The resulting minimum required sample size to achieve 85% power was 14 for 2-sided group-1 and group-2 errors 5% [(Effect size 0.60, Critical F 4.10, Ndf 2, Ddf 10, Power (1-β err pob) = 0.86 (86% power)].