CoffeeNCT07024940
Short-term Effects of Caffeine and a Multi-ingredient Pre-workout on Exercise Performance
A study testing Ethiopian Coffee and Supplement in people with Coffee and Anthocyanins, run by St. Louis University at 1 site in United States.
Trial summary
The investigators are conducting a study to see how a commercial pre-workout supplement, coffee, and water affect how well people perform. The study is conducted across four visits. The first visit is to assess the participants, and the next three visits are for testing. Read more about this study
- Who is it for?
- Ages 18 to 35 yearsHealthy volunteers can take part
- Conditions the study is for
- Coffee, Anthocyanins, Exercise Performance
- What is being tested?
- Ethiopian Coffee and Supplement
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 12 participants
- Where
- 1 siteUnited States
- Study dates
- 23 Apr 2025to 30 Jul 2026
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 35 years
- All sexes
- Healthy volunteers accepted
- Adult
Who may be able to join
- Are a healthy male or female adult aged 18-35 years old
- Regularly engaged in resistance training or aerobic exercise at least 3 times per week, at least 30 minutes a week for the past 6 months
- Able to provide written informed consent
Who cannot join
- Have a history of or currently have a cardiovascular or metabolic disease (e.g., diabetes, prediabetes, hypertension, arrhythmia, heart attack, stroke, non-alcoholic fatty liver disease, chronic kidney disease)
- History of seizure disorder
- Currently taking prescription medications (e.g., beta-blockers, antidepressants, anti-anxiety, pain killers)
- Currently pregnant, breastfeeding, pregnant within the past year, or a chance of being pregnant
- Recent participation in another clinical trial
- Any sensitivities to caffeine, coffee, berries, maltodextrin, monk fruit extract, or any known food allergies
- Currently undertaking serious exercise training (6 or more days of the week)
- Recently started taking creatine or beta-alanine daily within the past 3 months. (If greater than 3 months taken consistently is okay)
Trial locations and contacts
1 site in 1 country.
About this study
Official title: Acute Effects of Coffee, Anthocyanin-Caffeine Supplement and Placebo on Exercise Performance Markers
The investigators are conducting a study to see how a commercial pre-workout supplement, coffee, and water affect how well people perform. The study is conducted across four visits. The first visit is to assess the participants, and the next three visits are for testing. Each participant will drink a randomly assigned beverage 45 minutes before testing. Throughout the study, the participants will complete three testing days-each at least three days apart-so they can try all three drinks. The first test is the Wingate test, where the participants pedal as fast as they can for 30 seconds against a set resistance. The second test is a 1-mile treadmill run/walk on an incline, where the participant tries to complete the distance as fast as possible. The third test measures reaction time using light-up buttons to see how quickly a participant responds by touching them. These tests will help determine if any of the drinks improve performance. The results can help people choose the best drink for their workouts or races.
Read the detailed description
This study is being conducted to evaluate how a commercial pre-workout supplement with multiple ingredients and coffee affects how people perform during exercise testing. The study takes place over 4 separate lab visits. The first lab visit is an assessment for each participant to help define the population to be tested. Then, three testing visits will be identical in design but will differ in the intervention given to the participant. Each participant will consume the pre-workout, coffee, and a placebo in a randomized order. At the beginning of each visit, the participant will consume their assigned beverage. The participants will then start a warm-up after 30 minutes of consuming the assigned beverage. After the 10-minute warm-up, the participant will rest for five minutes before conducting the testing. In the testing visits, participants will conduct a 1-mile treadmill run, in which the participant will determine their speed using a 1% incline. This allows the participant to control the speed, allowing them to try as hard as they can. The next test will be a Wingate cycle ergometer test. The test contains a 20-second warm-up followed by a three-second period where the participant will pedal as fast as they can until the test begins, then they will do an all-out effort for 30 seconds. The participant will then conduct an agility reaction time test to measure any benefits the interventions may have on cognitive function.
- Conditions
- Coffee, Anthocyanins, Exercise Performance
- Being tested
- Ethiopian Coffee, Supplement, Water (Placebo)
- Keywords
- Coffee, Caffeine, Wingate, Anthocyanin-Caffeine, 1-mile, VO2 max, pre-workout, supplement
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Ethiopian CoffeeOther
Ethiopian Coffee is used as a real-world example of one of the most common drinks in the world.
SupplementDietary supplement
A pre-workout supplement that contains anthocyanins and caffeine as ergogenic aids
Water (Placebo)Other
Bottled water acting as the placebo
| Study group | Role | What they receive |
|---|---|---|
| Ethiopian Coffee | Experimental | A light roast Ethiopian Coffee is used based on previous research using the same technique for brewing. The coffee is brewed using the Aeropress coffee maker and the Timemore C3Pro hand grinder to grind the coffee. The recipe uses 15 grams of ground coffee set at the same grind setting (16 clicks) and 200 grams of water to have a target cup size of 175 mL plus 50 grams of ice to rapidly cool down the drink for consumption. Receives: Other: Ethiopian Coffee |
| Anthocyanin-Caffeine | Experimental | An anthocyanin-caffeine pre-workout supplement containing 120 mg of anthocyanins from New Zealand Blackcurrant berries, along with 120 mg of caffeine. This single-serve packet is stirred into 8 ounces of water and served. Receives: Dietary Supplement: Supplement |
| Water | Placebo comparator | Water used as a constant water intake across all three arms Receives: Other: Water (Placebo) |
What is being measured
The primary outcome is the main question the study is designed to answer.
Change in anaerobic power measured by a 30-second all-out effort on a cycle ergometer
The primary outcome measured is the change in anaerobic power measured by a 30-second all-out effort on a cycle ergometer using the Wingate testing. This test will determine whether the interventions confer any performance benefit compared to the placebo.
Measured: At 45 minutes post-dose for each intervention condition
Secondary outcomes (2)
1-mile Treadmill time trial
The 1-mile treadmill time trial will help determine if either of the interventions allows for a faster time, translating to an increase in physical performance.
Measured: At 45 minutes post-dose for each intervention condition
Agility Reaction Time
Agility reaction time will be measured with light-up buttons, where participants will need to touch the light to measure time. This test will identify if either intervention can reduce reaction time, which may reflect improvements in neuromotor performance.
Measured: At 45 minutes post-dose for each intervention condition
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted17 Jun 2025
- Study start23 Apr 2025
- Primary completion30 Jul 2026
- Study completion30 Jul 2026
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Publications (7)Papers the sponsor has linked to this study.
- Other trials for CoffeeStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- None (open label)
- Enrolment
- 12 participants (actual)
Sponsor and investigators
- Lead sponsor
- St. Louis University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Hossein Rafiei, St. Louis University
- Investigators
- Hossein Rafiei, PhD, Principal investigator, St. Louis University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07024940
- Sponsor's protocol ID
- 34611
- Status last verified
- Jul 2026
- First submitted
- 2 Jun 2025
- Last updated
- 28 Sep 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Publications
Castañeda-Babarro A. The Wingate Anaerobic Test, a Narrative Review of the Protocol Variables That Affect the Results Obtained. Applied Sciences. 2021; 11(16):7417. https://doi.org/10.3390/app11167417
Lindsey ZR, Williams JR, Burgess JS, Moore NT, Splichal PM. Caffeine content in filter coffee brews as a function of degree of roast and extraction yield. Sci Rep. 2024 Nov 25;14(1):29126. doi: 10.1038/s41598-024-80385-3.
PubMed 39582028Jardine WT, Aisbett B, Kelly MK, Burke LM, Ross ML, Condo D, Periard JD, Carr AJ. The Effect of Pre-Exercise Hyperhydration on Exercise Performance, Physiological Outcomes and Gastrointestinal Symptoms: A Systematic Review. Sports Med. 2023 Nov;53(11):2111-2134. doi: 10.1007/s40279-023-01885-2. Epub 2023 Jul 25.
PubMed 37490269Peacock OJ, Thompson D, Stokes KA. Voluntary drinking behaviour, fluid balance and psychological affect when ingesting water or a carbohydrate-electrolyte solution during exercise. Appetite. 2012 Feb;58(1):56-63. doi: 10.1016/j.appet.2011.08.023. Epub 2011 Sep 29.
PubMed 21986184Lee JD, Sterrett LE, Guth LM, Konopka AR, Mahon AD. The effect of pre-exercise carbohydrate supplementation on anaerobic exercise performance in adolescent males. Pediatr Exerc Sci. 2011 Aug;23(3):344-54. doi: 10.1123/pes.23.3.344.
PubMed 21881155Kaczka P, Batra A, Kubicka K, Maciejczyk M, Rzeszutko-Belzowska A, Pezdan-Sliz I, Michalowska-Sawczyn M, Przydzial M, Plonka A, Cieszczyk P, Huminska-Lisowska K, Zajac T. Effects of Pre-Workout Multi-Ingredient Supplement on Anaerobic Performance: Randomized Double-Blind Crossover Study. Int J Environ Res Public Health. 2020 Nov 9;17(21):8262. doi: 10.3390/ijerph17218262.
PubMed 33182295Krzysztof, Wioleta Dorobek, Katarzyna Stankiewicz, Janusz Świeczkowski-Feiz. The Role of Caffeine in Enhancing Physical Performance: From Metabolism to Muscle Function. Journal of Education, Health and Sport. 2024;59. doi:10.12775/JEHS.2024.59.010
Study at a glance
From the registry record, last updated by the sponsor on 28 Sep 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Acute Effects of Coffee, Anthocyanin-Caffeine Supplement and Placebo on Exercise Performance Markers
- ClinicalTrials.gov ID
- NCT07024940
- Acronym / sponsor ID
- 34611
- Conditions
- Coffee, Anthocyanins, Exercise Performance
- Keywords
- Coffee, Caffeine, Wingate, Anthocyanin-Caffeine, 1-mile, VO2 max, pre-workout, supplement
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 12 participants (actual)
- Lead sponsor
- St. Louis UniversityUniversity, hospital or other
- Study start
- 23 Apr 2025 (actual)
- Primary completion
- 30 Jul 2026 (actual)
- Study completion
- 30 Jul 2026 (actual)
- First posted
- 17 Jun 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The investigators are conducting a study to see how a commercial pre-workout supplement, coffee, and water affect how well people perform. The study is conducted across four visits. The first visit is to assess the participants, and the next three visits are for testing. Each participant will drink a randomly assigned beverage 45 minutes before testing. Throughout the study, the participants will complete three testing days-each at least three days apart-so they can try all three drinks. The first test is the Wingate test, where the participants pedal as fast as they can for 30 seconds against a set resistance. The second test is a 1-mile treadmill run/walk on an incline, where the participant tries to complete the distance as fast as possible. The third test measures reaction time using light-up buttons to see how quickly a participant responds by touching them. These tests will help determine if any of the drinks improve performance. The results can help people choose the best drink for their workouts or races.
Detailed description
This study is being conducted to evaluate how a commercial pre-workout supplement with multiple ingredients and coffee affects how people perform during exercise testing. The study takes place over 4 separate lab visits. The first lab visit is an assessment for each participant to help define the population to be tested. Then, three testing visits will be identical in design but will differ in the intervention given to the participant. Each participant will consume the pre-workout, coffee, and a placebo in a randomized order. At the beginning of each visit, the participant will consume their assigned beverage. The participants will then start a warm-up after 30 minutes of consuming the assigned beverage. After the 10-minute warm-up, the participant will rest for five minutes before conducting the testing. In the testing visits, participants will conduct a 1-mile treadmill run, in which the participant will determine their speed using a 1% incline. This allows the participant to control the speed, allowing them to try as hard as they can. The next test will be a Wingate cycle ergometer test. The test contains a 20-second warm-up followed by a three-second period where the participant will pedal as fast as they can until the test begins, then they will do an all-out effort for 30 seconds. The participant will then conduct an agility reaction time test to measure any benefits the interventions may have on cognitive function.
Study design
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- None (open label)
- Number of arms
- 3
| Arm | Type | Receives |
|---|---|---|
| Ethiopian Coffee | Experimental | Other: Ethiopian CoffeeA light roast Ethiopian Coffee is used based on previous research using the same technique for brewing. The coffee is brewed using the Aeropress coffee maker and the Timemore C3Pro hand grinder to grind the coffee. The recipe uses 15 grams of ground coffee set at the same grind setting (16 clicks) and 200 grams of water to have a target cup size of 175 mL plus 50 grams of ice to rapidly cool down the drink for consumption. |
| Anthocyanin-Caffeine | Experimental | Dietary Supplement: SupplementAn anthocyanin-caffeine pre-workout supplement containing 120 mg of anthocyanins from New Zealand Blackcurrant berries, along with 120 mg of caffeine. This single-serve packet is stirred into 8 ounces of water and served. |
| Water | Placebo comparator | Other: Water (Placebo)Water used as a constant water intake across all three arms |
Who may qualify
- Ages
- 18 to 35 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (3 listed)
- Are a healthy male or female adult aged 18-35 years old
- Regularly engaged in resistance training or aerobic exercise at least 3 times per week, at least 30 minutes a week for the past 6 months
- Able to provide written informed consent
Key exclusion criteria (8 listed)
- Have a history of or currently have a cardiovascular or metabolic disease (e.g., diabetes, prediabetes, hypertension, arrhythmia, heart attack, stroke, non-alcoholic fatty liver disease, chronic kidney disease)
- History of seizure disorder
- Currently taking prescription medications (e.g., beta-blockers, antidepressants, anti-anxiety, pain killers)
- Currently pregnant, breastfeeding, pregnant within the past year, or a chance of being pregnant
- Recent participation in another clinical trial
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Ethiopian CoffeeEthiopian Coffee is used as a real-world example of one of the most common drinks in the world. | Other | Ethiopian Coffee |
| SupplementA pre-workout supplement that contains anthocyanins and caffeine as ergogenic aids | Dietary supplement | Anthocyanin-Caffeine |
| Water (Placebo)Bottled water acting as the placebo | Other | Water |
Primary outcome
Change in anaerobic power measured by a 30-second all-out effort on a cycle ergometer
Time frame: At 45 minutes post-dose for each intervention condition
The primary outcome measured is the change in anaerobic power measured by a 30-second all-out effort on a cycle ergometer using the Wingate testing. This test will determine whether the interventions confer any performance benefit compared to the placebo.
2 secondary outcomes
1-mile Treadmill time trial
Time frame: At 45 minutes post-dose for each intervention condition
Agility Reaction Time
Time frame: At 45 minutes post-dose for each intervention condition
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 28 Sep 2026.
- BackgroundCastañeda-Babarro A. The Wingate Anaerobic Test, a Narrative Review of the Protocol Variables That Affect the Results Obtained. Applied Sciences. 2021; 11(16):7417. https://doi.org/10.3390/app11167417
- Background: PubMed 39582028Lindsey ZR, Williams JR, Burgess JS, Moore NT, Splichal PM. Caffeine content in filter coffee brews as a function of degree of roast and extraction yield. Sci Rep. 2024 Nov 25;14(1):29126. doi: 10.1038/s41598-024-80385-3.
- Background: PubMed 37490269Jardine WT, Aisbett B, Kelly MK, Burke LM, Ross ML, Condo D, Periard JD, Carr AJ. The Effect of Pre-Exercise Hyperhydration on Exercise Performance, Physiological Outcomes and Gastrointestinal Symptoms: A Systematic Review. Sports Med. 2023 Nov;53(11):2111-2134. doi: 10.1007/s40279-023-01885-2. Epub 2023 Jul 25.
- Background: PubMed 21986184Peacock OJ, Thompson D, Stokes KA. Voluntary drinking behaviour, fluid balance and psychological affect when ingesting water or a carbohydrate-electrolyte solution during exercise. Appetite. 2012 Feb;58(1):56-63. doi: 10.1016/j.appet.2011.08.023. Epub 2011 Sep 29.
- Background: PubMed 21881155Lee JD, Sterrett LE, Guth LM, Konopka AR, Mahon AD. The effect of pre-exercise carbohydrate supplementation on anaerobic exercise performance in adolescent males. Pediatr Exerc Sci. 2011 Aug;23(3):344-54. doi: 10.1123/pes.23.3.344.
- Background: PubMed 33182295Kaczka P, Batra A, Kubicka K, Maciejczyk M, Rzeszutko-Belzowska A, Pezdan-Sliz I, Michalowska-Sawczyn M, Przydzial M, Plonka A, Cieszczyk P, Huminska-Lisowska K, Zajac T. Effects of Pre-Workout Multi-Ingredient Supplement on Anaerobic Performance: Randomized Double-Blind Crossover Study. Int J Environ Res Public Health. 2020 Nov 9;17(21):8262. doi: 10.3390/ijerph17218262.
- BackgroundKrzysztof, Wioleta Dorobek, Katarzyna Stankiewicz, Janusz Świeczkowski-Feiz. The Role of Caffeine in Enhancing Physical Performance: From Metabolism to Muscle Function. Journal of Education, Health and Sport. 2024;59. doi:10.12775/JEHS.2024.59.010
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
Hossein Rafiei, PhD principal investigator
St. Louis University
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Ethiopian Coffee and Supplement in people with Coffee and Anthocyanins, run by St. Louis University at 1 site in United States.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 3 inclusion and 8 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 28 Sep 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in United States. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Change in anaerobic power measured by a 30-second all-out effort on a cycle ergometer (time frame: At 45 minutes post-dose for each intervention condition). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
On the ClinicalTrials.gov record for NCT07024940, which also lists 7 linked publications.
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 28 Sep 2026; AzTrials last refreshed its copy on 6 Nov 2026.