Mouth BreathingPM26NCT07755111
Effect of Pranayamic Breathing and Tongue Exercises on Oral Microbiota in Children
A study testing Pranayamic Breathing and Tongue Exercises in people with Mouth Breathing and Oral Microbiota, run by Gulcin Ozsen at 1 site in Turkey (Türkiye).
Trial summary
This study aims to evaluate the effects of pranayamic breathing techniques and tongue exercises on oral microbiota in children aged 7-12 years. Children will be grouped according to their breathing patterns (mouth or nasal breathing), and a structured breathing and tongue exercise program will be applied. Read more about this study
- Who is it for?
- Ages 7 to 12 yearsHealthy volunteers can take part
- Conditions the study is for
- Mouth Breathing, Oral Microbiota
- What is being tested?
- Pranayamic Breathing and Tongue Exercises
- Type of treatment
- Pill or oral medicine, Lifestyle or therapy programme
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 80 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 28 Mar 2026to 15 Dec 2026
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 7 to 12 years
- All sexes
- Healthy volunteers accepted
- Child
Who may be able to join
- Children aged 7-12 years with either mouth breathing or nasal breathing patterns
- Willingness of both the child and their legal guardian to participate in the study
- Children who are cooperative and able to comply with the exercise protocol
Who cannot join
- Refusal of the child or legal guardian to participate in the study
- Presence of active dental caries or periodontal disease
- Presence of any systemic disease or regular medication use
- Use of orthodontic appliances
- Use of antibiotics or probiotics within the last 4 weeks
Trial locations and contacts
1 site in 1 country. Contact the study team or a site near you to ask about taking part.
Turkey (Türkiye)1 site, 1 recruiting
- Recruiting
Dentistun Oral and Dental Health Center, Istanbul Health and Technology University
Istanbul, Bahcelievler, 34180
Gulcin Ozsen, DDS, +90 555 711 99 39, gulcinozsen@gmail.com
About this study
Official title: The Effects of Pranayamic Breathing Techniques and Tongue Exercises on Oral Microbiota in Children Aged 7-12 Years
This study aims to evaluate the effects of pranayamic breathing techniques and tongue exercises on oral microbiota in children aged 7-12 years. Children will be grouped according to their breathing patterns (mouth or nasal breathing), and a structured breathing and tongue exercise program will be applied. Saliva samples will be collected before and after the intervention to analyze changes in oral microbiota. The study will include approximately 80 participants and is expected to last for 6 months. There are no anticipated risks for participants. The findings of this study may contribute to a better understanding of the relationship between breathing patterns, oral exercises, and oral health in children.
Read the detailed description
This study is designed to investigate the effects of pranayamic breathing techniques, tongue exercises, and tongue cleaning on oral microbiota in children aged 7-12 years. Participants will be classified based on their breathing patterns as mouth breathers or nasal breathers. Each group will undergo a structured intervention program that includes specific breathing techniques, tongue exercises, and, in selected subgroups, tongue cleaning over a defined period.
Saliva samples will be collected from all participants before and after the intervention. These samples will be analyzed using microbiological methods to evaluate changes in oral microbiota composition. The study aims to compare the microbiological outcomes between different breathing patterns and to assess the potential benefits of the applied interventions.
Informed consent will be obtained from all participants and their legal guardians prior to participation. All collected samples and data will be anonymized to ensure participant confidentiality.
This research is expected to provide insights into the relationship between breathing habits, oral functions, tongue hygiene practices, and oral microbial balance in children. The findings may contribute to preventive and therapeutic approaches in pediatric dentistry, particularly in managing the effects of mouth breathing on oral health.
- Conditions
- Mouth Breathing, Oral Microbiota
- Being tested
- Pranayamic Breathing and Tongue Exercises
- Keywords
- Oral Microbiota, Pranayamic Breathing, humming exercise, Mouth Breathing, Tongue Exercises
- Medical subject headings
- Mouth Breathing
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Pranayamic Breathing and Tongue ExercisesBehavioural
Participants will perform a structured program including pranayamic breathing techniques and tongue exercises, and, in selected subgroups, tongue cleaning. The program will be applied regularly over the study period, and saliva samples will be collected before and after the intervention to assess changes in oral microbiota.
| Study group | Role | What they receive |
|---|---|---|
| Mouth Breathing Group | Experimental | Participants with mouth breathing habits will be included in this group. A structured program consisting of pranayamic breathing techniques, tongue exercises, and, in selected subgroups, tongue cleaning will be applied. Saliva samples will be collected before and after the intervention to evaluate changes in oral microbiota. Receives: Behavioral: Pranayamic Breathing and Tongue Exercises |
| Nasal Breathing Group | Experimental | Participants with nasal breathing habits will be included in this group. A structured program consisting of pranayamic breathing techniques, tongue exercises, and, in selected subgroups, tongue cleaning will be applied. Saliva samples will be collected before and after the intervention to evaluate changes in oral microbiota. Receives: Behavioral: Pranayamic Breathing and Tongue Exercises |
What is being measured
The primary outcome is the main question the study is designed to answer.
Change in oral microbiota alpha diversity as measured by the Shannon diversity index from 16S rRNA gene sequencing of saliva samples
This outcome evaluates the effect of the intervention (pranayamic breathing exercises, tongue exercises, and tongue-cleaning practice) on the diversity of the oral microbiota. Alpha diversity will be assessed from saliva samples using 16S rRNA gene sequencing and quantified using the Shannon diversity index. The mean Shannon index at baseline will be compared with the value after the 8-week intervention, and the change from baseline will be reported.
Measured: 8 weeks
Secondary outcomes (1)
Salivary Nitric Oxide Levels
Salivary nitric oxide levels will be measured using test strips before and after the intervention.
Measured: 8 weeks
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted10 Aug 2026
- Study start28 Mar 2026
- Primary completion15 Sep 2026 (estimated)
- Study completion15 Dec 2026 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Mouth BreathingStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Treatment
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Masking
- Single blind
- Who is masked
- Outcomes assessor
- Enrolment
- 80 participants (estimated)
Sponsor and investigators
- Lead sponsor
- Gulcin Ozsen
- Sponsor type
- University, hospital or other
- Responsible party
- Sponsor-investigator, Gulcin Ozsen, Istanbul Health and Technology University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07755111
- Sponsor's protocol ID
- PRANAYAMIC-MICROBIOTA-26
- Acronym
- PM26
- Status last verified
- Jun 2026
- First submitted
- 19 Jun 2026
- Last updated
- 10 Aug 2026
- Results posted
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No
Study at a glance
From the registry record, last updated by the sponsor on 10 Aug 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Effects of Pranayamic Breathing Techniques and Tongue Exercises on Oral Microbiota in Children Aged 7-12 Years
- ClinicalTrials.gov ID
- NCT07755111
- Acronym / sponsor ID
- PM26 / PRANAYAMIC-MICROBIOTA-26
- Conditions
- Mouth Breathing, Oral Microbiota
- Keywords
- Oral Microbiota, Pranayamic Breathing, humming exercise, Mouth Breathing, Tongue Exercises
- Study type
- Interventional
- Recruitment status
- Recruiting
- Enrolment
- 80 participants (estimated)
- Lead sponsor
- Gulcin OzsenUniversity, hospital or other
- Study start
- 28 Mar 2026 (actual)
- Primary completion
- 15 Sep 2026 (estimated)
- Study completion
- 15 Dec 2026 (estimated)
- First posted
- 10 Aug 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This study aims to evaluate the effects of pranayamic breathing techniques and tongue exercises on oral microbiota in children aged 7-12 years. Children will be grouped according to their breathing patterns (mouth or nasal breathing), and a structured breathing and tongue exercise program will be applied. Saliva samples will be collected before and after the intervention to analyze changes in oral microbiota. The study will include approximately 80 participants and is expected to last for 6 months. There are no anticipated risks for participants. The findings of this study may contribute to a better understanding of the relationship between breathing patterns, oral exercises, and oral health in children.
Detailed description
This study is designed to investigate the effects of pranayamic breathing techniques, tongue exercises, and tongue cleaning on oral microbiota in children aged 7-12 years. Participants will be classified based on their breathing patterns as mouth breathers or nasal breathers. Each group will undergo a structured intervention program that includes specific breathing techniques, tongue exercises, and, in selected subgroups, tongue cleaning over a defined period.
Saliva samples will be collected from all participants before and after the intervention. These samples will be analyzed using microbiological methods to evaluate changes in oral microbiota composition. The study aims to compare the microbiological outcomes between different breathing patterns and to assess the potential benefits of the applied interventions.
Informed consent will be obtained from all participants and their legal guardians prior to participation. All collected samples and data will be anonymized to ensure participant confidentiality.
This research is expected to provide insights into the relationship between breathing habits, oral functions, tongue hygiene practices, and oral microbial balance in children. The findings may contribute to preventive and therapeutic approaches in pediatric dentistry, particularly in managing the effects of mouth breathing on oral health.
Study design
- Primary purpose
- Treatment
- Allocation
- Non-randomised
- Intervention model
- Parallel groups
- Masking
- Single blind (outcomes assessor)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| Mouth Breathing Group | Experimental | Behavioral: Pranayamic Breathing and Tongue ExercisesParticipants with mouth breathing habits will be included in this group. A structured program consisting of pranayamic breathing techniques, tongue exercises, and, in selected subgroups, tongue cleaning will be applied. Saliva samples will be collected before and after the intervention to evaluate changes in oral microbiota. |
| Nasal Breathing Group | Experimental | Behavioral: Pranayamic Breathing and Tongue ExercisesParticipants with nasal breathing habits will be included in this group. A structured program consisting of pranayamic breathing techniques, tongue exercises, and, in selected subgroups, tongue cleaning will be applied. Saliva samples will be collected before and after the intervention to evaluate changes in oral microbiota. |
Who may qualify
- Ages
- 7 to 12 years
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Child
Key inclusion criteria (3 listed)
- Children aged 7-12 years with either mouth breathing or nasal breathing patterns
- Willingness of both the child and their legal guardian to participate in the study
- Children who are cooperative and able to comply with the exercise protocol
Key exclusion criteria (5 listed)
- Refusal of the child or legal guardian to participate in the study
- Presence of active dental caries or periodontal disease
- Presence of any systemic disease or regular medication use
- Use of orthodontic appliances
- Use of antibiotics or probiotics within the last 4 weeks
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Pranayamic Breathing and Tongue ExercisesParticipants will perform a structured program including pranayamic breathing techniques and tongue exercises, and, in selected subgroups, tongue cleaning. The program will be applied regularly over the study period, and saliva samples will be collected before and after the intervention to assess changes in oral microbiota. | Behavioural | Mouth Breathing Group, Nasal Breathing Group |
Primary outcome
Change in oral microbiota alpha diversity as measured by the Shannon diversity index from 16S rRNA gene sequencing of saliva samples
Time frame: 8 weeks
This outcome evaluates the effect of the intervention (pranayamic breathing exercises, tongue exercises, and tongue-cleaning practice) on the diversity of the oral microbiota. Alpha diversity will be assessed from saliva samples using 16S rRNA gene sequencing and quantified using the Shannon diversity index. The mean Shannon index at baseline will be compared with the value after the 8-week intervention, and the change from baseline will be reported.
1 secondary outcome
Salivary Nitric Oxide Levels
Time frame: 8 weeks
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 10 Aug 2026.
- Individual participant dataSharing planned: No
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Pranayamic Breathing and Tongue Exercises in people with Mouth Breathing and Oral Microbiota, run by Gulcin Ozsen at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 3 inclusion and 5 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: looking for participants now, as of the sponsor's last update on 10 Aug 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
Contact Gulcin Ozsen, DDS (+90 555 711 99 39, gulcinozsen@gmail.com), or a site near the patient. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Change in oral microbiota alpha diversity as measured by the Shannon diversity index from 16S rRNA gene sequencing of saliva samples (time frame: 8 weeks). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 10 Aug 2026.