Exercise-Induced Muscle StressMuscreenNCT07774221
EIMD and Microgreens
A study testing Mixed Microgreens and Wheatgrass Juice and Lettuce in people with Exercise-Induced Muscle Stress, run by Çanakkale Onsekiz Mart University at 1 site in Turkey (Türkiye).
Trial summary
This study investigated whether a mixed microgreens intervention could modulate biochemical responses to strenuous eccentric exercise. The findings suggest that microgreens consumption was associated with lower post-exercise levels of several muscle damage and inflammatory biomarkers, particularly CK, LDH, AST, TNF-α… Read more about this study
- Who is it for?
- Ages 20 to 25 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Exercise-Induced Muscle Stress
- What is being tested?
- Mixed Microgreens and Wheatgrass Juice and Lettuce
- Could I receive a placebo?
- No, everyone receives an active treatment
- How many people
- 16 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 5 Dec 2025to 14 Dec 2026
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 20 to 25 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Male football players aged 20-25 years. Active participation in an amateur football team in Çanakkale, Türkiye. Physically active according to the physical activity criteria referenced by the American College of Sports Medicine and the World Health Organization.
- No health condition that would prevent participation in the study procedures. Willingness to comply with the study protocol and scheduled assessments. Provision of written informed consent.
Who cannot join
- Musculoskeletal or joint injury, particularly involving the lower extremities, within the previous 6 months.
- Regular use of medication. History of chronic disease, including cardiovascular disease, diabetes, or other metabolic disorders.
- No regular participation in a structured training program during the preceding year.
- History of alcohol or tobacco use. Inability or unwillingness to comply with the study procedures.
Trial locations and contacts
1 site in 1 country.
About this study
Official title: Sporcularda Yüksek Şiddetli Egzersiz Sonrası Mikro Yeşillerin Kullanımının Toparlanma Üzerine Etkilerinin İncelenmesi
This study investigated whether a mixed microgreens intervention could modulate biochemical responses to strenuous eccentric exercise. The findings suggest that microgreens consumption was associated with lower post-exercise levels of several muscle damage and inflammatory biomarkers, particularly CK, LDH, AST, TNF-α, and CRP, while effects on myoglobin and IL-6 were limited. Further controlled studies are needed to confirm these findings and clarify their biological significance.
- Conditions
- Exercise-Induced Muscle Stress
- Being tested
- Mixed Microgreens and Wheatgrass Juice, Lettuce
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Mixed Microgreens and Wheatgrass JuiceDietary supplement
Participants received a mixed preparation of black radish, red radish, broccoli, and kale microgreens at a total daily dose of 60 g, divided into three 20-g administrations consumed in the morning, at noon, and in the evening. Participants also consumed 30 mL of wheatgrass juice each morning during the intervention period. The intervention was administered over four cycles, with each cycle consisting of 5 consecutive days followed by a 2-day interval. The final intervention cycle coincided with the exercise period.
LettuceDietary supplement
Participants assigned to the control group received lettuce as the control green food according to the study protocol and underwent the same exercise and assessment procedures as the intervention group.
| Study group | Role | What they receive |
|---|---|---|
| Control group | Experimental | Participants assigned to the control group received lettuce as the control green food and underwent the same exercise protocol, blood sampling schedule, and study procedures as the intervention group. Blood samples were collected at baseline and 24, 48, and 72 h after exercise. Receives: Dietary Supplement: Lettuce |
| Microgreens Intervention Group | Experimental | Participants received a mixed microgreens intervention consisting of black radish, red radish, broccoli, and kale microgreens at a total daily dose of 60 g, divided into three 20-g administrations consumed in the morning, at noon, and in the evening. Participants also consumed 30 mL of wheatgrass juice each morning. The intervention was administered over four cycles, with each cycle consisting of 5 consecutive days followed by a 2-day interval. The final intervention cycle coincided with the exercise period. Participants underwent the same standardized eccentric plyometric exercise protocol and blood sampling schedule as the control group. Receives: Dietary Supplement: Mixed Microgreens and Wheatgrass Juice |
What is being measured
The primary outcome is the main question the study is designed to answer.
Serum biochemical responses following eccentric plyometric exercise
Between-group differences in serum biochemical responses following the standardized high-intensity eccentric plyometric exercise protocol. Muscle-related biochemical markers included creatine kinase, lactate dehydrogenase, aspartate aminotransferase, and myoglobin. Inflammatory markers included interleukin-6, tumor necrosis factor-alpha, and C-reactive protein. Measurements were obtained at baseline and 24, 48, and 72 hours after exercise, and the temporal responses were compared between the control and plant-based intervention groups.
Measured: Baseline, 24, 48, and 72 hours after exercise
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted19 Aug 2026
- Study start5 Dec 2025
- Primary completion6 Jan 2026
- Study completion14 Dec 2026 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Exercise-Induced Muscle StressStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Enrolment
- 16 participants (actual)
Sponsor and investigators
- Lead sponsor
- Çanakkale Onsekiz Mart University
- Sponsor type
- University, hospital or other
- Responsible party
- Principal investigator, Mahmut Açak, Çanakkale Onsekiz Mart University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07774221
- Sponsor's protocol ID
- TDK-2025-5220
- Other IDs
- TDK-2025-5220
- Acronym
- Muscreen
- Status last verified
- Jan 2026
- First submitted
- 14 Aug 2026
- Last updated
- 19 Aug 2026
- Results posted
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- Yes: The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
Study at a glance
From the registry record, last updated by the sponsor on 19 Aug 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- Sporcularda Yüksek Şiddetli Egzersiz Sonrası Mikro Yeşillerin Kullanımının Toparlanma Üzerine Etkilerinin İncelenmesi
- ClinicalTrials.gov ID
- NCT07774221
- Acronym / sponsor ID
- Muscreen / TDK-2025-5220
- Conditions
- Exercise-Induced Muscle Stress
- Study type
- Interventional
- Recruitment status
- Active, not recruiting
- Enrolment
- 16 participants (actual)
- Lead sponsor
- Çanakkale Onsekiz Mart UniversityUniversity, hospital or other
- Study start
- 5 Dec 2025 (actual)
- Primary completion
- 6 Jan 2026 (actual)
- Study completion
- 14 Dec 2026 (estimated)
- First posted
- 19 Aug 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
This study investigated whether a mixed microgreens intervention could modulate biochemical responses to strenuous eccentric exercise. The findings suggest that microgreens consumption was associated with lower post-exercise levels of several muscle damage and inflammatory biomarkers, particularly CK, LDH, AST, TNF-α, and CRP, while effects on myoglobin and IL-6 were limited. Further controlled studies are needed to confirm these findings and clarify their biological significance.
Study design
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- None (open label)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| Control group | Experimental | Dietary Supplement: LettuceParticipants assigned to the control group received lettuce as the control green food and underwent the same exercise protocol, blood sampling schedule, and study procedures as the intervention group. Blood samples were collected at baseline and 24, 48, and 72 h after exercise. |
| Microgreens Intervention Group | Experimental | Dietary Supplement: Mixed Microgreens and Wheatgrass JuiceParticipants received a mixed microgreens intervention consisting of black radish, red radish, broccoli, and kale microgreens at a total daily dose of 60 g, divided into three 20-g administrations consumed in the morning, at noon, and in the evening. Participants also consumed 30 mL of wheatgrass juice each morning. The intervention was administered over four cycles, with each cycle consisting of 5 consecutive days followed by a 2-day interval. The final intervention cycle coincided with the exercise period. Participants underwent the same standardized eccentric plyometric exercise protocol and blood sampling schedule as the control group. |
Who may qualify
- Ages
- 20 to 25 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (2 listed)
- Male football players aged 20-25 years. Active participation in an amateur football team in Çanakkale, Türkiye. Physically active according to the physical activity criteria referenced by the American College of Sports Medicine and the World Health Organization.
- No health condition that would prevent participation in the study procedures. Willingness to comply with the study protocol and scheduled assessments. Provision of written informed consent.
Key exclusion criteria (4 listed)
- Musculoskeletal or joint injury, particularly involving the lower extremities, within the previous 6 months.
- Regular use of medication. History of chronic disease, including cardiovascular disease, diabetes, or other metabolic disorders.
- No regular participation in a structured training program during the preceding year.
- History of alcohol or tobacco use. Inability or unwillingness to comply with the study procedures.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Mixed Microgreens and Wheatgrass JuiceParticipants received a mixed preparation of black radish, red radish, broccoli, and kale microgreens at a total daily dose of 60 g, divided into three 20-g administrations consumed in the morning, at noon, and in the evening. Participants also consumed 30 mL of wheatgrass juice each morning during the intervention period. The intervention was administered over four cycles, with each cycle consisting of 5 consecutive days followed by a 2-day interval. The final intervention cycle coincided with the exercise period. | Dietary supplement | Microgreens Intervention Group |
| LettuceParticipants assigned to the control group received lettuce as the control green food according to the study protocol and underwent the same exercise and assessment procedures as the intervention group. | Dietary supplement | Control group |
Primary outcome
Serum biochemical responses following eccentric plyometric exercise
Time frame: Baseline, 24, 48, and 72 hours after exercise
Between-group differences in serum biochemical responses following the standardized high-intensity eccentric plyometric exercise protocol. Muscle-related biochemical markers included creatine kinase, lactate dehydrogenase, aspartate aminotransferase, and myoglobin. Inflammatory markers included interleukin-6, tumor necrosis factor-alpha, and C-reactive protein. Measurements were obtained at baseline and 24, 48, and 72 hours after exercise, and the temporal responses were compared between the control and plant-based intervention groups.
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 19 Aug 2026.
- Individual participant dataSharing planned: Yes. The datasets generated and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Mixed Microgreens and Wheatgrass Juice and Lettuce in people with Exercise-Induced Muscle Stress, run by Çanakkale Onsekiz Mart University at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 2 inclusion and 4 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: under way, not looking for participants, as of the sponsor's last update on 19 Aug 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Serum biochemical responses following eccentric plyometric exercise (time frame: Baseline, 24, 48, and 72 hours after exercise). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 19 Aug 2026.