Exercise PerformanceNCT07048912
The Effect of Acute Beetroot Juice Supplementation on Anaerobic Performance in Football Players
A study testing Beetroot Juice (BJ) in people with Exercise Performance and Supplementation, run by Melike Nur Eroğlu at 1 site in Turkey (Türkiye).
Trial summary
The aim of this clinical trial is to examine the effects of two different natural fruit-vegetable blend beverages on short-duration, high-intensity exercise performance and muscle oxygenation in young male football players. The study also investigates how these beverages influence certain metabolic responses. Read more about this study
- Who is it for?
- Ages 18 to 19 years, men onlyHealthy volunteers can take part
- Conditions the study is for
- Exercise Performance, Supplementation
- What is being tested?
- Beetroot Juice (BJ)
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 16 participants
- Where
- 1 siteTurkey (Türkiye)
- Study dates
- 1 Jul 2024to 25 Jul 2024
- Phase
- Interventional
- Are there results?
- None posted in the registry
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 to 19 years
- Men only
- Healthy volunteers accepted
- Adult
Who may be able to join
- Healthy males over 18 years old
- Performing resistance exercise at least three times per week
- At least 5 years of football training experience
Who cannot join
- Contraindications to Wingate exercise
- Presence of cardiometabolic disease
- Current use of ergogenic supplementation
- Smokers
Trial locations and contacts
1 site in 1 country.
Turkey (Türkiye)1 site
Sakarya University of Applied Sciences, Faculty of Sport Sciences, Exercise and Sports Sciences Education, Application and Research Centre
Sakarya
About this study
The aim of this clinical trial is to examine the effects of two different natural fruit-vegetable blend beverages on short-duration, high-intensity exercise performance and muscle oxygenation in young male football players. The study also investigates how these beverages influence certain metabolic responses. The main questions it seeks to answer are:
- Does one of the beverages improve peak and mean power output during the Wingate test?
- How do these beverages affect muscle oxygenation and post-exercise recovery?
- Researchers will compare the effects of the two beverages on performance and metabolic markers.
Read more
Participants:
- Consume one of the two natural beverages 2.5 hours before testing (assigned at random)
- Perform a 30-second high-intensity Wingate cycling test
- Undergo measurements of muscle oxygenation, heart rate, blood pressure, blood lactate, and blood glucose during and after exercise
- Maintain consistent dietary and physical activity routines and avoid certain foods prior to testing
- Conditions
- Exercise Performance, Supplementation
- Being tested
- Beetroot Juice (BJ), Blackcurrant Juice (Placebo)
- Keywords
- beetroot juice, muscle oxygenation, nitrate, football, high intensity efforts
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Beetroot Juice (BJ)Dietary supplement
This intervention involves a single 140 mL dose of a commercially available concentrated beetroot juice supplement (Beet-It Pro Elite Shot, James White Drinks Ltd., UK), containing approximately 12.8 mmol (800 mg) of dietary nitrate. Participants consume the juice 2.5 hours prior to performing a 30-second Wingate anaerobic test. The purpose of this intervention is to examine its acute effects on anaerobic performance, muscle oxygenation, and metabolic responses.
Blackcurrant Juice (Placebo)Dietary supplement
This intervention involves a single 140 mL dose of a low-calorie blackcurrant juice (Pfanner, Austria) with negligible nitrate content (<0.1 mmol). The placebo is matched to beetroot juice in appearance, taste, and packaging to ensure double-blind conditions. It is consumed 2.5 hours before the Wingate test and serves as the control condition.
| Study group | Role | What they receive |
|---|---|---|
| Beetroot Juice (BJ) | Experimental | Participants in this arm consumed 140 mL of nitrate-rich beetroot juice (~12.8 mmol nitrate) as a single acute dose, approximately 2.5 hours before the performance assessments. The supplementation was administered in the morning under supervision, and participants were instructed to maintain their usual diet but avoid additional nitrate-rich foods 24 hours prior to testing. Receives: Dietary Supplement: Beetroot Juice (BJ); Dietary Supplement: Blackcurrant Juice (Placebo) |
| Placebo | Experimental | Participants in this arm consumed 140 mL of blackcurrant juice containing negligible nitrate (<0.1 mmol) as a single acute dose, approximately 2.5 hours before the performance assessments. The supplementation was administered in the morning under supervision, and participants followed the same dietary instructions as the experimental group, avoiding nitrate-rich foods 24 hours prior to testing. Receives: Dietary Supplement: Beetroot Juice (BJ); Dietary Supplement: Blackcurrant Juice (Placebo) |
What is being measured
The primary outcome is the main question the study is designed to answer.
Mean power output during the 30-second Wingate anaerobic test
Average power (in watts) generated during the 30-second Wingate cycling test will be compared between beetroot juice and placebo conditions to assess anaerobic performance.
Measured: Day 1 (2.5 hours after supplementation, during Wingate test)
Secondary outcomes (1)
Muscle oxygen saturation (SmO₂)
SmO₂ will be assessed using Near-Infrared Spectroscopy (NIRS) on the vastus lateralis muscle. Measurements will be taken continuously before, during, and after the 30-second Wingate anaerobic test to evaluate changes in muscle oxygenation in response to the intervention.
Measured: Day 1 (pre-, during, and post-exercise on the day of Wingate test)
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted3 Jul 2025
- Study start1 Jul 2024
- Primary completion21 Jul 2024
- Study completion25 Jul 2024
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Exercise PerformanceStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Triple blind
- Who is masked
- Participant, Investigator, Outcomes assessor
- Enrolment
- 16 participants (actual)
Sponsor and investigators
- Lead sponsor
- Melike Nur Eroğlu
- Sponsor type
- University, hospital or other
- Responsible party
- Sponsor-investigator, Melike Nur Eroğlu, Sakarya Applied Sciences University
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07048912
- Sponsor's protocol ID
- SUBU-EROGLU-2025-01
- Status last verified
- Jun 2025
- First submitted
- 11 Jun 2025
- Last updated
- 3 Jul 2025
- Results posted
- No
- Data monitoring committee
- Yes
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- No: We prefer not to share the Individual Participant Data (IPD) to protect participant privacy and comply with data protection and ethical guidelines.
Study at a glance
From the registry record, last updated by the sponsor on 3 Jul 2025. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Effect of Acute Beetroot Juice Supplementation on Anaerobic Performance in Football Players
- ClinicalTrials.gov ID
- NCT07048912
- Acronym / sponsor ID
- SUBU-EROGLU-2025-01
- Conditions
- Exercise Performance, Supplementation
- Keywords
- beetroot juice, muscle oxygenation, nitrate, football, high intensity efforts
- Study type
- Interventional
- Recruitment status
- Completed
- Enrolment
- 16 participants (actual)
- Lead sponsor
- Melike Nur EroğluUniversity, hospital or other
- Study start
- 1 Jul 2024 (actual)
- Primary completion
- 21 Jul 2024 (actual)
- Study completion
- 25 Jul 2024 (actual)
- First posted
- 3 Jul 2025 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The aim of this clinical trial is to examine the effects of two different natural fruit-vegetable blend beverages on short-duration, high-intensity exercise performance and muscle oxygenation in young male football players. The study also investigates how these beverages influence certain metabolic responses. The main questions it seeks to answer are:
- Does one of the beverages improve peak and mean power output during the Wingate test?
- How do these beverages affect muscle oxygenation and post-exercise recovery?
- Researchers will compare the effects of the two beverages on performance and metabolic markers.
Participants:
- Consume one of the two natural beverages 2.5 hours before testing (assigned at random)
- Perform a 30-second high-intensity Wingate cycling test
- Undergo measurements of muscle oxygenation, heart rate, blood pressure, blood lactate, and blood glucose during and after exercise
- Maintain consistent dietary and physical activity routines and avoid certain foods prior to testing
Study design
- Primary purpose
- Other
- Allocation
- Randomised
- Intervention model
- Crossover
- Masking
- Triple blind (participant, investigator, outcomes assessor)
- Number of arms
- 2
| Arm | Type | Receives |
|---|---|---|
| Beetroot Juice (BJ) | Experimental | Dietary Supplement: Beetroot Juice (BJ), Dietary Supplement: Blackcurrant Juice (Placebo)Participants in this arm consumed 140 mL of nitrate-rich beetroot juice (~12.8 mmol nitrate) as a single acute dose, approximately 2.5 hours before the performance assessments. The supplementation was administered in the morning under supervision, and participants were instructed to maintain their usual diet but avoid additional nitrate-rich foods 24 hours prior to testing. |
| Placebo | Experimental | Dietary Supplement: Beetroot Juice (BJ), Dietary Supplement: Blackcurrant Juice (Placebo)Participants in this arm consumed 140 mL of blackcurrant juice containing negligible nitrate (<0.1 mmol) as a single acute dose, approximately 2.5 hours before the performance assessments. The supplementation was administered in the morning under supervision, and participants followed the same dietary instructions as the experimental group, avoiding nitrate-rich foods 24 hours prior to testing. |
Who may qualify
- Ages
- 18 to 19 years
- Sex
- Male only
- Healthy volunteers
- Accepted
- Age groups
- Adult
Key inclusion criteria (3 listed)
- Healthy males over 18 years old
- Performing resistance exercise at least three times per week
- At least 5 years of football training experience
Key exclusion criteria (4 listed)
- Contraindications to Wingate exercise
- Presence of cardiometabolic disease
- Current use of ergogenic supplementation
- Smokers
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Beetroot Juice (BJ)This intervention involves a single 140 mL dose of a commercially available concentrated beetroot juice supplement (Beet-It Pro Elite Shot, James White Drinks Ltd., UK), containing approximately 12.8 mmol (800 mg) of dietary nitrate. Participants consume the juice 2.5 hours prior to performing a 30-second Wingate anaerobic test. The purpose of this intervention is to examine its acute effects on anaerobic performance, muscle oxygenation, and metabolic responses. | Dietary supplement | Beetroot Juice (BJ), Placebo |
| Blackcurrant Juice (Placebo)This intervention involves a single 140 mL dose of a low-calorie blackcurrant juice (Pfanner, Austria) with negligible nitrate content (<0.1 mmol). The placebo is matched to beetroot juice in appearance, taste, and packaging to ensure double-blind conditions. It is consumed 2.5 hours before the Wingate test and serves as the control condition. | Dietary supplement | Beetroot Juice (BJ), Placebo |
Primary outcome
Mean power output during the 30-second Wingate anaerobic test
Time frame: Day 1 (2.5 hours after supplementation, during Wingate test)
Average power (in watts) generated during the 30-second Wingate cycling test will be compared between beetroot juice and placebo conditions to assess anaerobic performance.
1 secondary outcome
Muscle oxygen saturation (SmO₂)
Time frame: Day 1 (pre-, during, and post-exercise on the day of Wingate test)
Results and safety
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 3 Jul 2025.
- Individual participant dataSharing planned: No. We prefer not to share the Individual Participant Data (IPD) to protect participant privacy and comply with data protection and ethical guidelines.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part. This study is not recruiting at the moment.
The record lists no contact. Use the official record to reach the sponsor.
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Beetroot Juice (BJ) in people with Exercise Performance and Supplementation, run by Melike Nur Eroğlu at 1 site in Turkey (Türkiye).
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 3 inclusion and 4 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: finished, as of the sponsor's last update on 3 Jul 2025. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Turkey (Türkiye). Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
The study is completed, but results are not available in the source record. That does not mean the treatment did or did not work.
How can a doctor refer a patient or contact the research team?
The record lists no contact. The official record names the sponsor, who can be asked directly. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Mean power output during the 30-second Wingate anaerobic test (time frame: Day 1 (2.5 hours after supplementation, during Wingate test)). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 3 Jul 2025.