Dental PlaqueNCT07364565
The Antiplaque and Antigingivitis Effect of Pure Green Tea Mouth Wash Compared to Placebo and Essential Oils Mouth Washes
A study testing Pure Green Tea Mouthwash, Listerine Total Care Mouthwash and Listerine Green Tea Mouthwash in people with Dental Plaque and Patient Reported Outcome, run by King Abdullah University Hospital at 1 site in Jordan.
Trial summary
The goal of this clinical trial is to to evaluate the antiplaque and antigingivitis effect of pure green tea mouthwash compared to placebo and essential oils mouthwashes in healthy patients. Read more about this study
- Who is it for?
- Ages 18 years and olderHealthy volunteers can take part
- Conditions the study is for
- Dental Plaque, Patient Reported Outcome, Gingival Inflammation and Bleeding
- What is being tested?
- Pure Green Tea Mouthwash, Listerine Total Care Mouthwash and Listerine Green Tea Mouthwash
- Type of treatment
- Pill or oral medicine
- Could I receive a placebo?
- Yes, some participants may receive a placebo
- How many people
- 44 participants
- Where
- 1 siteJordan
- Study dates
- 1 Dec 2025to Dec 2026
- Phase
- Interventional
- Are there results?
- Not yet: the study is under way
Key requirements
Who can take part, as listed by the sponsor. Only the study team can confirm whether you are eligible, so take this list to your doctor.
- Ages 18 years and older
- All sexes
- Healthy volunteers accepted
- Adult, Older adult
Who may be able to join
- Non-smokers
- Systemically healthy
- At least 24 teeth in the functional dentition, excluding third molars
- Clinical diagnosis of periodontal health (no periodontal pockets > 3 mm, bleeding on probing percentage < 10%, and no attachment loss).
Who cannot join
- Smokers.
- Medically compromised patients.
- Pregnant woman.
- Allergies to green tea or EO.
- Any condition that might affect the periodontal tissues.
- Patients with any carious lesions (initial or active).
- Patients who have overhang restorations, poorly designed crowns or bridges, and ortho retainers.
- Use of systemic antibiotics or anti-inflammatory medications within 3 months prior to enrolment.
Trial locations and contacts
1 site in 1 country. Contact the study team or a site near you to ask about taking part.
Jordan1 site, 1 recruiting
- Recruiting
Jordan University of Science and Technology, Faculty of Dentistry
Irbid, Irbid Governorate, 22110
Lana Bader, DClinDent Perio, +962791200956, lmbader4@just.edu.jo
About this study
Official title: The Antiplaque and Antigingivitis Effect of Pure Green Tea Mouth Wash Compared to Placebo and Essential Oils Mouth Washes: A Double Blinded Randomized Controlled Clinical Trial.
The goal of this clinical trial is to to evaluate the antiplaque and antigingivitis effect of pure green tea mouthwash compared to placebo and essential oils mouthwashes in healthy patients.
The main questions it aims to answer are:
Read more
- Does pure green tea mouthwash effectively reduce dental plaque?
- Does pure green tea mouthwash reduce gingival inflammation?
Researchers will compare pure green tea mouthwash to Listerine Total Care, Listerine Green Tea, and placebo to see if there is a difference in plaque accumulation and gum inflammation reduction.
Participants will be prohibited from using any sort of oral hygiene parctice for the whole study period (3 weeks). Instead, they will rinse with 10 ml of their assigned mouthwash twice daily for 30 seconds. Plaque index, gingival index, and staining index will be examined at multiple intervals and participants will rate their sense of oral freshness and good breath.
Read the detailed description
- Study Design
This study will be a double-blinded, randomized, controlled, clinical trial with a parallel group design. Participants will be randomly allocated into four groups:
Group P: placebo (pure water with a flavored additive) solution. Group T1: pure green tea mouthwash. Group T2: Listerine Total Care mouthwash. Group T3: Listerine Green Tea mouthwash.
All participants will be instructed to rinse with 10 ml of the assigned mouthwash for 30 seconds twice daily over a three-week experimental period. The study will follow the experimental gingivitis model, during which participants will refrain from all forms of mechanical plaque control.
- Sample Size A total of 44 dental participants, aged between 18-50 years, will be recruited. Each group will consist of 11 participants, providing a power of 80% to detect a difference of 0.2 in mean plaque index values between groups, with a p-value < 0.05 and a β-error of 20%.
- Ethical Considerations All participants will be informed about the study objectives, methodology, and possible risks. Written informed consent will be obtained before participation. All procedures will follow ethical standards and the principles of the Declaration of Helsinki.
- Data Collection and Clinical Assessments Clinical examinations will be performed at baseline (T0), day 7 (T1), day 14 (T2), and day 21 (T3). The following parameters will be recorded: plaque index, gingival index, staining index, and patients reported outcome in regards to their perciption of freshness and good breath.
After the experimental period (T3), participants will receive a professional cleaning and fluoride application, and will be followed for two additional weeks to ensure the restoration of oral health.
- Randomization and Blinding Randomization will be performed using a computer-generated sequence by an independent researcher. The mouthwash samples will be provided in coded and unlabeled containers to ensure blinding of both participants and the examiner. A third party will be responsible about desposing the assigned mouth wash to the patients.
- Adverse Events Monitoring Participants will be closely monitored throughout the study for any possible adverse effects such as gingival irritation, sensitivity, or allergic reactions. Any incident will be documented and managed according to the study protocol. Any participant who experience an adverse event will be excluded from the study.
- Conditions
- Dental Plaque, Patient Reported Outcome, Gingival Inflammation and Bleeding, Gingival Inflammation, Gingival Bleeding, Gingivitis
- Being tested
- Pure Green Tea Mouthwash, Listerine Total Care Mouthwash, Listerine Green Tea Mouthwash, Placebo Mouthwash
- Keywords
- Green tea, Gingival inflammation, Plaque index, Essential oils, Mouthwash, Bleeding on probing, Camellia sinensis, Herbal medicine, Periodontal health
- Medical subject headings
- Dental Plaque, Gingivitis, Hemorrhage, Gingival Hemorrhage
What participants receive
What participants receive. In a randomised study each person is assigned to one group by chance.
Pure Green Tea MouthwashDrug
Participants in this group will use a mouthwash prepared from pure green tea extract. The solution will be brewed by infusing 1 g of green tea leaves in 100 ml of boiling deionized water for 20 minutes, cooled, filtered, and stored in brown glass bottles. Participants will rinse with 10 ml of the mouthwash twice daily for 30 seconds over 3 weeks. They should not use any form of oral hygiene during the study period.
Listerine Total Care MouthwashDrug
Participants in this group will use Listerine Total Care essential oils mouthwash, containing eucalyptol, menthol, methyl salicylate, and thymol. All participants will rinse with 10 ml twice daily for 30 seconds for 3 weeks. They should not use any form of oral hygiene during the study period. This will serve as an active control to compare the efficacy of essential oils with the pure green tea formulation and placebo.
Listerine Green Tea MouthwashDrug
Participants will use Listerine Green Tea mouthwash, an essential oil-based product enhanced with green tea extract and fluoride. All participants will rinse with 10 ml twice daily for 30 seconds over 3 weeks. They should not use any form of oral hygiene during the study period. This will allow comparison between a commercial green tea-containing mouthwash and the pure green tea solution.
Placebo MouthwashOther
Participants will rinse with 10 ml of a placebo solution consisting of pure water with a flavored additive to mimic taste and color. They should not use any form of oral hygiene during the study period. The solution contains no active anti-inflammatory ingredients. The placebo serves to determine baseline changes in plaque and gingivitis without active intervention.
| Study group | Role | What they receive |
|---|---|---|
| Pure Green Tea Mouthwash | Experimental | Participants in this group will use a mouthwash prepared from pure green tea extract. The solution will be brewed by infusing 1 g of green tea leaves in 100 ml of boiling deionized water for 20 minutes, cooled, filtered, and stored in brown glass bottles. Receives: Drug: Pure Green Tea Mouthwash |
| Listerine Total Care Mouthwash | Active comparator | Participants in this group will use Listerine Total Care essential oils mouthwash, containing eucalyptol, menthol, methyl salicylate, and thymol. Receives: Drug: Listerine Total Care Mouthwash |
| Listerine Green Tea Mouthwash | Active comparator | Participants will use Listerine Green Tea mouthwash, an essential oil-based product enhanced with green tea extract. Receives: Drug: Listerine Green Tea Mouthwash |
| Placebo Mouthwash | Placebo comparator | The solution contains only water and additive flavor with no active anti-inflammatory ingredients. Receives: Other: Placebo Mouthwash |
What is being measured
The primary outcome is the main question the study is designed to answer.
Turesky modification of the Quigley-Hein plaque index (TM-QHPI)
will be recorded at six aspects on each tooth (mesialbuccal, midbuccal, distobuccal, mesiolingual, midlingual, and distolingual).
0: No plaque.
- Separate flecks of plaque at the gumline (cervical margin).
- A thin, continuous band (up to 1 mm) at the gumline.
- A band wider than 1 mm but covering less than one-third of the tooth crown.
- Plaque covering one-third to two-thirds of the crown.
- Plaque covering two-thirds or more of the crown.
Measured: On days 0, 7, 14, and 21
Secondary outcomes (3)
Gingival Index (GI)
Gingival inflammation will be evaluated using the Löe \& Silness Gingival Index (GI). It will be recorded at six aspects on each tooth (mesialbuccal, midbuccal, distobuccal, mesiolingual, midlingual, and distolingual).
Gingival index 0: Healthy gums. Gingival index 1: Mild discolouration and oedematous gingiva. No bleeding on probing.
Gingival index 2: Red, oedematous and shiny gingiva. There is bleeding on probing.
Gingival index 3: Red, oedematous and ulcerated gingiva. There is spontaneous bleeding.
Measured: On days 0, 7, 14, and 21
The Discoloration Index (DI)
will be recorded at six aspects on each tooth (mesialbuccal, midbuccal, distobuccal, mesiolingual, midlingual, and distolingual).
Score 0: No discoloration, clean and polished tooth surface, natural appearance in color.
Score1: Slight yellowing discoloration, yellowish film over the entire extent of the clinical crown, slight brownish discoloration along the gingival margin.
Score 2: Moderate brownish discoloration on the interproximal surfaces and in the apical third of the clinical crown.
Score 3: Heavy brown and black discoloration over the entire extent of the tooth surface, black discoloration predominantly on the interproximal surfaces
Measured: On days 0, 7, 14, and 21
Patient-reported outcome
A self-reported outcome assessing participants' perceived feeling of oral freshness, good breath and cleanliness after using the assigned mouthwash. Each participant will rate their experience on a Visual Analogue Scale (VAS) from 0 ("not fresh or clean at all") to 10 ("very fresh and clean").
Measured: On days 7, 14, and 21
Timeline
From the start of the study to its completion; the mark is today. Estimated dates are the sponsor’s plan.
- First posted23 Jan 2026
- Study start1 Dec 2025
- Primary completionDec 2026 (estimated)
- Study completionDec 2026 (estimated)
Resources
- Official record on ClinicalTrials.govThe registry entry this page is built from, with its full change history.
- Other trials for Dental PlaqueStudies of the same condition, open and under way.
- What the trial phases meanHow a treatment moves from first tests in people to approval.
More details
For doctors and researchers: how the study is designed, who runs it, and its registry IDs.
Study design
- Study type
- Interventional
- Phase
- Phase N/A
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Model description
- This is a double-blinded, parallel group, randomised, controlled, clinical trial with four arms: pure green tea mouthwash, Listerine Total Care, Listerine Green Tea, and placebo. Participants will rinse twice daily for three weeks. They will be prohibited from all forms of mechanical plaque control during the study period.
- Masking
- Quadruple blind
- Who is masked
- Participant, Care provider, Investigator, Outcomes assessor
- Enrolment
- 44 participants (estimated)
Sponsor and investigators
- Lead sponsor
- King Abdullah University Hospital
- Sponsor type
- University, hospital or other
- Collaborators
- Jordan University of Science and Technology
- Responsible party
- Principal investigator, Lana Bader, Jordan University of Science and Technology
- Investigators
- Lana Bader, DClinDent Perio, Study director, Jordan University of Science and TechnologyDana Abdullah, MClinDent Perio, Principal investigator, Jordan University of Science and Technology
IDs, dates and publications
- ClinicalTrials.gov ID
- NCT07364565
- Sponsor's protocol ID
- 425/2025
- Other IDs
- 2025/185-1
- Status last verified
- Jan 2026
- First submitted
- 29 Dec 2025
- Last updated
- 23 Jan 2026
- Results posted
- No
- Data monitoring committee
- No
- FDA-regulated drug
- No
- FDA-regulated device
- No
- Individual data sharing
- Yes: Individual participant data for plaque index, gingival index, discolouration index, and patient-reported outcomes will be shared. Data will be stripped of all identifiers to protect participant privacy.
Study at a glance
From the registry record, last updated by the sponsor on 23 Jan 2026. Recruitment status is as the sponsor reported it: confirm with the study team.
- Official title
- The Antiplaque and Antigingivitis Effect of Pure Green Tea Mouth Wash Compared to Placebo and Essential Oils Mouth Washes: A Double Blinded Randomized Controlled Clinical Trial.
- ClinicalTrials.gov ID
- NCT07364565
- Acronym / sponsor ID
- 425/2025
- Conditions
- Dental Plaque, Patient Reported Outcome, Gingival Inflammation and Bleeding, Gingival Inflammation, Gingival Bleeding, Gingivitis
- Keywords
- Green tea, Gingival inflammation, Plaque index, Essential oils, Mouthwash, Bleeding on probing, Camellia sinensis, Herbal medicine
- Study type
- Interventional
- Recruitment status
- Recruiting
- Enrolment
- 44 participants (estimated)
- Lead sponsor
- King Abdullah University HospitalUniversity, hospital or other
- Collaborators
- Jordan University of Science and Technology
- Study start
- 1 Dec 2025 (actual)
- Primary completion
- Dec 2026 (estimated)
- Study completion
- Dec 2026 (estimated)
- First posted
- 23 Jan 2026 (actual)
- Results
- Not posted in the source record
Clinical summary
The research question as the sponsor registered it. This describes what the study plans to test, not what it found.
The goal of this clinical trial is to to evaluate the antiplaque and antigingivitis effect of pure green tea mouthwash compared to placebo and essential oils mouthwashes in healthy patients.
The main questions it aims to answer are:
- Does pure green tea mouthwash effectively reduce dental plaque?
- Does pure green tea mouthwash reduce gingival inflammation?
Researchers will compare pure green tea mouthwash to Listerine Total Care, Listerine Green Tea, and placebo to see if there is a difference in plaque accumulation and gum inflammation reduction.
Participants will be prohibited from using any sort of oral hygiene parctice for the whole study period (3 weeks). Instead, they will rinse with 10 ml of their assigned mouthwash twice daily for 30 seconds. Plaque index, gingival index, and staining index will be examined at multiple intervals and participants will rate their sense of oral freshness and good breath.
Detailed description
- Study Design
This study will be a double-blinded, randomized, controlled, clinical trial with a parallel group design. Participants will be randomly allocated into four groups:
Group P: placebo (pure water with a flavored additive) solution. Group T1: pure green tea mouthwash. Group T2: Listerine Total Care mouthwash. Group T3: Listerine Green Tea mouthwash.
All participants will be instructed to rinse with 10 ml of the assigned mouthwash for 30 seconds twice daily over a three-week experimental period. The study will follow the experimental gingivitis model, during which participants will refrain from all forms of mechanical plaque control.
- Sample Size A total of 44 dental participants, aged between 18-50 years, will be recruited. Each group will consist of 11 participants, providing a power of 80% to detect a difference of 0.2 in mean plaque index values between groups, with a p-value < 0.05 and a β-error of 20%.
- Ethical Considerations All participants will be informed about the study objectives, methodology, and possible risks. Written informed consent will be obtained before participation. All procedures will follow ethical standards and the principles of the Declaration of Helsinki.
- Data Collection and Clinical Assessments Clinical examinations will be performed at baseline (T0), day 7 (T1), day 14 (T2), and day 21 (T3). The following parameters will be recorded: plaque index, gingival index, staining index, and patients reported outcome in regards to their perciption of freshness and good breath.
After the experimental period (T3), participants will receive a professional cleaning and fluoride application, and will be followed for two additional weeks to ensure the restoration of oral health.
- Randomization and Blinding Randomization will be performed using a computer-generated sequence by an independent researcher. The mouthwash samples will be provided in coded and unlabeled containers to ensure blinding of both participants and the examiner. A third party will be responsible about desposing the assigned mouth wash to the patients.
- Adverse Events Monitoring Participants will be closely monitored throughout the study for any possible adverse effects such as gingival irritation, sensitivity, or allergic reactions. Any incident will be documented and managed according to the study protocol. Any participant who experience an adverse event will be excluded from the study.
Study design
- Primary purpose
- Treatment
- Allocation
- Randomised
- Intervention model
- Parallel groups
- Masking
- Quadruple blind (participant, care provider, investigator, outcomes assessor)
- Number of arms
- 4
- Model description
- This is a double-blinded, parallel group, randomised, controlled, clinical trial with four arms: pure green tea mouthwash, Listerine Total Care, Listerine Green Tea, and placebo. Participants will rinse twice daily for three weeks. They will be prohibited from all forms of mechanical plaque control during the study period.
| Arm | Type | Receives |
|---|---|---|
| Pure Green Tea Mouthwash | Experimental | Drug: Pure Green Tea MouthwashParticipants in this group will use a mouthwash prepared from pure green tea extract. The solution will be brewed by infusing 1 g of green tea leaves in 100 ml of boiling deionized water for 20 minutes, cooled, filtered, and stored in brown glass bottles. |
| Listerine Total Care Mouthwash | Active comparator | Drug: Listerine Total Care MouthwashParticipants in this group will use Listerine Total Care essential oils mouthwash, containing eucalyptol, menthol, methyl salicylate, and thymol. |
| Listerine Green Tea Mouthwash | Active comparator | Drug: Listerine Green Tea MouthwashParticipants will use Listerine Green Tea mouthwash, an essential oil-based product enhanced with green tea extract. |
| Placebo Mouthwash | Placebo comparator | Other: Placebo MouthwashThe solution contains only water and additive flavor with no active anti-inflammatory ingredients. |
Who may qualify
- Ages
- 18 years and older
- Sex
- All
- Healthy volunteers
- Accepted
- Age groups
- Adult, Older adult
Key inclusion criteria (4 listed)
- Non-smokers
- Systemically healthy
- At least 24 teeth in the functional dentition, excluding third molars
- Clinical diagnosis of periodontal health (no periodontal pockets > 3 mm, bleeding on probing percentage < 10%, and no attachment loss).
Key exclusion criteria (8 listed)
- Smokers.
- Medically compromised patients.
- Pregnant woman.
- Allergies to green tea or EO.
- Any condition that might affect the periodontal tissues.
Eligibility is decided by the study team after screening; this overview does not establish that a patient qualifies. Full inclusion and exclusion criteria.
Interventions
| Intervention | Type | Arms |
|---|---|---|
| Pure Green Tea MouthwashParticipants in this group will use a mouthwash prepared from pure green tea extract. The solution will be brewed by infusing 1 g of green tea leaves in 100 ml of boiling deionized water for 20 minutes, cooled, filtered, and stored in brown glass bottles. Participants will rinse with 10 ml of the mouthwash twice daily for 30 seconds over 3 weeks. They should not use any form of oral hygiene during the study period. | Drug | Pure Green Tea Mouthwash |
| Listerine Total Care MouthwashParticipants in this group will use Listerine Total Care essential oils mouthwash, containing eucalyptol, menthol, methyl salicylate, and thymol. All participants will rinse with 10 ml twice daily for 30 seconds for 3 weeks. They should not use any form of oral hygiene during the study period. This will serve as an active control to compare the efficacy of essential oils with the pure green tea formulation and placebo. | Drug | Listerine Total Care Mouthwash |
| Listerine Green Tea MouthwashParticipants will use Listerine Green Tea mouthwash, an essential oil-based product enhanced with green tea extract and fluoride. All participants will rinse with 10 ml twice daily for 30 seconds over 3 weeks. They should not use any form of oral hygiene during the study period. This will allow comparison between a commercial green tea-containing mouthwash and the pure green tea solution. | Drug | Listerine Green Tea Mouthwash |
| Placebo MouthwashParticipants will rinse with 10 ml of a placebo solution consisting of pure water with a flavored additive to mimic taste and color. They should not use any form of oral hygiene during the study period. The solution contains no active anti-inflammatory ingredients. The placebo serves to determine baseline changes in plaque and gingivitis without active intervention. | Other | Placebo Mouthwash |
Primary outcome
Turesky modification of the Quigley-Hein plaque index (TM-QHPI)
Time frame: On days 0, 7, 14, and 21
will be recorded at six aspects on each tooth (mesialbuccal, midbuccal, distobuccal, mesiolingual, midlingual, and distolingual). 0: No plaque. 1. Separate flecks of plaque at the gumline (cervical margin). 2. A thin, continuous band (up to 1 mm) at the gumline. 3. A band wider than 1 mm but covering less than one-third of the tooth crown. 4. Plaque covering one-third to two-thirds of the crown. 5. Plaque covering two-thirds or more of the crown.
3 secondary outcomes
Gingival Index (GI)
Time frame: On days 0, 7, 14, and 21
The Discoloration Index (DI)
Time frame: On days 0, 7, 14, and 21
Patient-reported outcome
Time frame: On days 7, 14, and 21
Results and safety
This study is still under way. Results are not available in the source record yet.
Research links
- Official record on ClinicalTrials.govLast updated by the sponsor 23 Jan 2026.
- Individual participant dataSharing planned: Yes. Individual participant data for plaque index, gingival index, discolouration index, and patient-reported outcomes will be shared. Data will be stripped of all identifiers to protect participant privacy.
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Referral and contact
AzTrials does not enrol participants or take referrals. Contact the study team or a site listed below; they decide who can take part.
Lana Bader, DClinDent Perio study director
Jordan University of Science and Technology
Dana Abdullah, MClinDent Perio principal investigator
Jordan University of Science and Technology
1 site in 1 country: see every site and its status.
Questions about this trial
What is this clinical trial studying?
A study testing Pure Green Tea Mouthwash, Listerine Total Care Mouthwash and Listerine Green Tea Mouthwash in people with Dental Plaque and Patient Reported Outcome, run by King Abdullah University Hospital at 1 site in Jordan.
The full registered description is in the About this study section and the official record.
Who may be eligible to take part?
The sponsor lists 4 inclusion and 8 exclusion criteria. Eligibility is decided by the study team after screening, so read the full criteria and contact a listed site.
Is this clinical trial currently recruiting?
The record says: looking for participants now, as of the sponsor's last update on 23 Jan 2026. Status can change between updates, so confirm with the study contact before making plans.
Where is the study taking place?
The record lists 1 site in Jordan. Whether a site is taking part right now should be confirmed with that site. See every site.
What are the possible benefits and risks?
They depend on the study and the treatment, and no study can promise a benefit. Some participants in this study may receive a placebo. The study team explains the known risks during informed consent, before anyone joins; the record itself does not list them.
Are results available for this study?
This study is still under way. Results are not available in the source record yet.
How can a doctor refer a patient or contact the research team?
Contact Lana Bader, DClinDent Perio (+962791200956, lmbader4@just.edu.jo), or a site near the patient. AzTrials does not enrol participants or take referrals.
What does the primary outcome measure?
Turesky modification of the Quigley-Hein plaque index (TM-QHPI) (time frame: On days 0, 7, 14, and 21). It is what the study was designed to measure, not a finding.
Where can I read the original study information?
Does a listing mean the treatment is approved or proven to work?
No. A listing means the study is registered. It does not show that the treatment is safe, works, or is approved by a regulator.
How often is this information updated?
AzTrials copies changed records from ClinicalTrials.gov every night and reloads the whole registry every week. The sponsor last updated this record on 23 Jan 2026.